Origin IGS: cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgattatttatcttttgctctatttaaaagacaaaaatacaaacttttcaggatatcctctcaccatcgcaacaaaacgtacaagacctgccggtcatacctgatcgcacaagccgacgatgcccctctctcctccgccttccgcctatccacaaccggattttcatttaccctgatacaattctgaaaattatcacgtatctttgcatccctaaacaaaagcaaaaagacg Mask Tandem Repeat Region ================================================ cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Find is-nt database================================================ Query_seq: TF3148:TF3149|TF3148:TF3149:hypoxanthine-guanine phosphoribosyltransferase:conserved hypothetical protein:->->:3384332..3384563 232 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF3148:TF3149|TF3148:TF3149:hypoxanthine-guanine phosphoribosyltransferase:conserved hypothetical protein:->->:3384332..3384563 232 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF3148:TF3149|TF3148:TF3149:hypoxanthine-guanine phosphoribosyltransferase:conserved hypothetical protein:->->:3384332..3384563 232 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 8 End: 169 ....... M L L F R D A K I R D N F Q N C I R V N E N P V V D R R K A E E R G A S S A C A I R Y D R Q V L Y V L L R W ............................................................... Protein_Len: 39 Strand: + Start: 66 End: 182 ................................................................. M K I R L W I G G R R R R E G H R R L V R S G M T G R S C T F C C D G E R I S .................................................. Protein_Len: 33 Strand: - Start: 132 End: 230 ................................................................................................................................... T H G A P R T R K T A I T L P Y G S F N T N K D K L Y L L L Y I M .. Protein_Len: 44 Strand: - Start: 49 End: 180 ................................................ F Q I L T F S F G T T S L R F A S S L P A D D A Q A I L Y S R C T K Y T K N R H H S S M .................................................... Protein_Len: 60 Strand: - Start: 2 End: 196 . K P I C L Y T I I K L I T D P Y I F I R N H I P P L R L L S P C R R S T R D P I V P L D Q V N Q Q S P S L I D Q F T Q M .................................... cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF3148:TF3149|TF3148:TF3149:hypoxanthine-guanine phosphoribosyltransferase:conserved hypothetical protein:->->:3384332..3384563 232 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Intra-Species Hit: Count: 1 Min: 1 Max: 232 Len: 232 Subject: tfor_TF3148_TF3149|hypoxanthine-guanine phosphoribosyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[3384332,3384563]|232 HSP 1 e-value: 1.0E-128 bit: 460.0 Len: 232 Query Start:1 Query End:232 Subject Strand: POSITIVE Subject Start: 1 Subject End: 232 cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca cgtctttttgcttttgtttagggatgcaaagatacgtgataattttcagaattgtatcagggtaaatgaaaatccggttgtggataggcggaaggcggaggagagaggggcatcgtcggcttgtgcgatcaggtatgaccggcaggtcttgtacgttttgttgcgatggtgagaggatatcctgaaaagtttgtatttttgtcttttaaatagagcaaaagataaataatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUCUUUUUGCUUUUGUUUAGGGAUGCAAAGAUACGUGAUAAUUUUCAGAAUUGUAUCAGGGUAAAUGAAAAUCCGGUUGUGGAUAGGCGGAAGGCGGAGGAGAGAGGGGCAUCGUCGGCUUGUGCGAUCAGGUAUGACCGGCAGGUCUUGUACGUUUUGUUGCGAUGGUGAGAGGAUAUCCUGAAAAGUUUGUAUUUUUGUCUUUUAAAUAGAGCAAAAGAUAAAUAAUCA .(((((((.(((((.((((((((..(((((((((((.((((.((((((..((((((.(((((((...((....(((((..(((((..(((..((((.((.((..........)).)).)))).))).)))...))..)))))....))...)))..)))).))))))..))))))..))))((....))..))))))))))).))))))))))))))))))))......... (-65.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUAUUUAUCUUUUGCUCUAUUUAAAAGACAAAAAUACAAACUUUUCAGGAUAUCCUCUCACCAUCGCAACAAAACGUACAAGACCUGCCGGUCAUACCUGAUCGCACAAGCCGACGAUGCCCCUCUCUCCUCCGCCUUCCGCCUAUCCACAACCGGAUUUUCAUUUACCCUGAUACAAUUCUGAAAAUUAUCACGUAUCUUUGCAUCCCUAAACAAAAGCAAAAAGACG ..........(((((((((...((((.....((((.((((....((((((((((..........((((((..............((((....))))..........((....)).).))))).....................(...((((......))))...)...............)))))))))).......)))).))))......))))....))).)))))).. (-31.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 506 119 74 91 49 tfor:TF0230|5end_hydrolase,_haloacid_dehalogenase-like_family_256982..257212_POSITIVE 489 157 111 51 4 tfor:TF1684|5end_zinc_protease_1797396..1797626_POSITIVE 488 126 89 157 119 tfor:TF1344|5end_conserved_hypothetical_protein_1425251..1425481_POSITIVE 482 120 74 127 69 tfor:TF3145|5end_conserved_hypothetical_protein;_possible_GTP_cyclohydrolase_I_family_3381254..3381484_POSITIVE 480 150 104 69 11 tfor:TF0301|5end_outer_membrane_protein,_TonB_dependent_receptor_325457..325687_POSITIVE 472 152 111 73 23 tfor:TF0810|5end_possible_outer_membrane_efflux_protein_868438..868668_POSITIVE 454 120 72 219 170 tfor:TF1995|5end_exonuclease_2147148..2147378_POSITIVE 447 148 101 186 118 tfor:TF0776|5end_ABC_transporter,_permease_component_824855..825085_POSITIVE 442 118 73 197 142 tfor:TF0712|5end_TPR-repeat-containing_protein_761348..761578_POSITIVE 439 120 72 195 133 tfor:TF1910|5end_nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase_2064001..2064231_POSITIVE 438 126 81 88 37 tfor:TF2911|5end_hypothetical_protein_3105871..3106101_POSITIVE 438 128 83 87 38 tfor:TF0610|5end_conserved_hypothetical_protein;_possible_phosphatidylglycerophosphate_synthase_643460..643690_POSITIVE 431 148 104 226 187 tfor:TF1037|5end_iron_(III)_ABC_transporter,_ATP-binding_protein_1098194..1098424_POSITIVE 430 120 73 61 3 tfor:TF2809|5end_conserved_hypothetical_protein;_possible_translation_factor_(SUA5)_2999832..3000062_POSITIVE 427 120 75 155 101 tfor:TF1919|5end_hypothetical_protein_2070037..2070267_POSITIVE 427 120 75 115 61 tfor:TF1917|5end_hypothetical_protein_2069997..2070227_POSITIVE 425 119 75 67 16 tfor:TF2895|5end_hypothetical_protein_3083936..3084166_POSITIVE 423 127 89 199 142 tfor:TF3029|5end_membrane_peptidase_3252803..3253033_POSITIVE 423 119 71 154 93 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 422 180 131 67 15 tfor:TF0094|5end_alpha-glucosidase_103561..103791_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 480 150 104 73 15 tfor:TF0300|3end_hypothetical_protein_325541..325691_POSITIVE 472 152 111 73 23 tfor:TF0809|3end_cation_efflux_protein_868518..868668_POSITIVE 441 124 89 150 114 tfor:TF2461|3end_outer_membrane_protein,_TonB-related_receptor_2641792..2641942_POSITIVE 438 126 81 93 42 tfor:TF2909|3end_GTP-binding_protein,_possible_GTP1/OBG_family_3105956..3106106_POSITIVE 427 120 75 143 89 tfor:TF1918|3end_hypothetical_protein_2070105..2070255_POSITIVE 416 126 86 139 91 tfor:TF1730|3end_diaminopimelate_decarboxylase_1855907..1856057_POSITIVE 414 120 74 58 1 tfor:TF2808|3end_bacterial_sugar_transferase_2999909..3000059_POSITIVE 413 119 73 91 42 tfor:TF0746|3end_possible_DNA_polymerase_III_792910..793060_POSITIVE 405 127 82 54 8 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 405 120 71 128 73 tfor:TF1204|3end_possible_metal-dependent_membrane_protease_1287461..1287611_POSITIVE 403 119 73 137 80 tfor:TF1663|3end_ABC_transporter,_permease_component_1778755..1778905_POSITIVE 401 128 88 81 43 tfor:TF1247|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_1330928..1331078_POSITIVE 396 120 75 123 71 tfor:TF2876|3end_cytosine_deaminase_3065664..3065814_POSITIVE 394 120 71 146 95 tfor:TF2991|3end_conserved_hypothetical_protein_3209107..3209257_POSITIVE 392 134 100 143 103 tfor:TF1323|3end_metallo-beta-lactamase_superfamily_protein_1403533..1403683_POSITIVE 390 120 72 92 47 tfor:TF0991|3end_probable_NAD(FAD)-dependent_dehydrogenase_1048334..1048484_POSITIVE 389 150 109 143 106 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 389 113 73 150 99 tfor:TF0710|3end_two_component_system_histidine_kinase_761385..761535_POSITIVE 388 137 93 57 5 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 387 150 105 54 10 tfor:TF2805|3end_zinc_protease_2996665..2996815_POSITIVE