Origin IGS: tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatttcttttacaaataaatcatactttatggaaagagcgcctttgtgaaaaggcgctctttttaccattttcttcttacatccatcacacagaaaatggcctcctgccgctccgttcgccggatacgatactacgcattgcgtctca Mask Tandem Repeat Region ================================================ tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Find is-nt database================================================ Query_seq: TF3064:TF3065|TF3064:TF3065:oxidoreductase, aldo/keto reductase family:enolase:->->:3291045..3291194 150 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF3064:TF3065|TF3064:TF3065:oxidoreductase, aldo/keto reductase family:enolase:->->:3291045..3291194 150 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF3064:TF3065|TF3064:TF3065:oxidoreductase, aldo/keto reductase family:enolase:->->:3291045..3291194 150 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 56 End: 148 ....................................................... M C D G C K K K M V K R A P F H K G A L S I K Y D L F V K E I .. Protein_Len: 44 Strand: - Start: 3 End: 134 .. S A I R L I T D P S R L P L L G N E T H H I Y S S F P L F L A K E C L R E K W L T H N M ................ Protein_Len: 43 Strand: - Start: 2 End: 130 . L R L A Y Y R I R R V S R C S A M K Q T I S T L L F H Y F S R R K V F A S K G Y L I M .................... tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -10.3 Tail_Score: -4.91084 Start: 81 End: 122 Full_Region: caaataaatcatact ttatggaaagagcgccttt gtga aaaggcgctctttttacca ttttcttcttacatc ................................................................................ttatggaaagagcgcctttgtgaaaaggcgctctttttacca............................ TransTerm Strand: + Conf: 100 HP_score: -15.4 Tail_Score: -3.66378 Start: 90 End: 113 Full_Region: AGAAAATGGTAAAAA GAGCGCCTTT TCAC AAAGGCGCTC TTTCCATAAAGTATG .........................................................................................GAGCGCCTTTTCACAAAGGCGCTC..................................... TransTerm Strand: - Conf: 100 HP_score: -15.4 Tail_Score: -4.75063 Start: 90 End: 113 Full_Region: catactttatggaaa gagcgccttt gtga aaaggcgctc tttttaccattttct .........................................................................................gagcgcctttgtgaaaaggcgctc..................................... Find igs database================================================ Query_seq: TF3064:TF3065|TF3064:TF3065:oxidoreductase, aldo/keto reductase family:enolase:->->:3291045..3291194 150 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: tfor_TF3064_TF3065|oxidoreductase, aldo/keto reductase family:enolase|POSITIVE:POSITIVE|[3291045,3291194]|150 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta tgagacgcaatgcgtagtatcgtatccggcgaacggagcggcaggaggccattttctgtgtgatggatgtaagaagaaaatggtaaaaagagcgccttttcacaaaggcgctctttccataaagtatgatttatttgtaaaagaaattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGACGCAAUGCGUAGUAUCGUAUCCGGCGAACGGAGCGGCAGGAGGCCAUUUUCUGUGUGAUGGAUGUAAGAAGAAAAUGGUAAAAAGAGCGCCUUUUCACAAAGGCGCUCUUUCCAUAAAGUAUGAUUUAUUUGUAAAAGAAAUUUA ....((((...))))....((((.((((.....))))))))..(((.((((((((((.....((....))....))))))))))...((((((((((((....)))))))))))))))...(((((.....))))).............. (-43.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUUCUUUUACAAAUAAAUCAUACUUUAUGGAAAGAGCGCCUUUGUGAAAAGGCGCUCUUUUUACCAUUUUCUUCUUACAUCCAUCACACAGAAAAUGGCCUCCUGCCGCUCCGUUCGCCGGAUACGAUACUACGCAUUGCGUCUCA ....................(((........((((((((((((((((....))))))))))))))))(((((((((.................)))))))))...........((((.....))))...)))...((((...)))).... (-37.13) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 433 46 1 120 72 tfor:TF2122|5end_NAD-specific_glutamate_dehydrogenase_2292734..2292964_POSITIVE 430 50 3 179 132 tfor:TF0969|5end_oxidoreductase_1019550..1019780_POSITIVE 420 67 25 227 179 tfor:TF0130|5end_conserved_hypothetical_protein_141835..142065_POSITIVE 414 69 21 225 180 tfor:TF0299|5end_hypothetical_protein_324821..325051_POSITIVE 414 47 3 76 27 tfor:TF0248|5end_hypothetical_protein_278249..278479_POSITIVE 402 70 21 177 111 tfor:TF2848|5end_cobalamin_biosynthesis_protein_3036368..3036598_POSITIVE 401 49 2 98 36 tfor:TF2218|5end_two-component_system_response_regulator_2390162..2390392_POSITIVE 398 50 6 215 166 tfor:TF1888|5end_RNA_polymerase_ECF-type_sigma_factor_2040940..2041170_POSITIVE 398 50 2 166 112 tfor:TF1586|5end_hypothetical_protein_1690971..1691201_POSITIVE 397 67 26 64 24 tfor:TF2744|5end_methionyl-tRNA_formyltransferase_2926297..2926527_POSITIVE 392 62 21 229 187 tfor:TF2819|5end_N-acetyl-gamma-glutamylphosphate_reductase_3008905..3009135_POSITIVE 391 60 11 189 147 tfor:TF2081|5end_phosphopantetheine_adenylyltransferase_2248475..2248705_POSITIVE 389 49 3 222 173 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 388 66 25 151 104 tfor:TF2705|5end_conserved_hypothetical_protein;_possible_SufE_Fe/S-cluster-related_protein_2876850..2877080_POSITIVE 384 51 12 47 1 tfor:TF0433|5end_60_kDa_inner_membrane_protein_453357..453587_POSITIVE 384 49 4 83 6 tfor:TF0244|5end_DNA_recombination_protein_274683..274913_POSITIVE 383 80 35 65 16 tfor:TF2244|5end_possible_mobilization_protein_2419425..2419655_POSITIVE 381 49 6 81 29 tfor:TF2791|5end_GTP-binding_protein_TypA_2975298..2975528_POSITIVE 379 50 3 217 172 tfor:TF0015|5end_outer_membrane_protein_(possible_immunogenic_lipoprotein)_9555..9785_POSITIVE 378 50 2 146 99 tfor:TF2887|5end_phosphomannomutase/phosphoglucomutase_3076327..3076557_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 433 46 1 66 18 tfor:TF2120|3end_holliday_junction_endonuclease_2292760..2292910_POSITIVE 414 47 3 95 46 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 402 49 4 50 5 tfor:TF3104|3end_outer_membrane_protein,_TonB_dependent_receptor_3337450..3337600_POSITIVE 388 66 25 142 95 tfor:TF2704|3end_ribonuclease_HII_2876921..2877071_POSITIVE 384 49 4 78 1 tfor:TF0243|3end_conserved_hypothetical_protein_274758..274908_POSITIVE 382 50 2 111 61 tfor:TF1848|3end_conserved_hypothetical_protein_1997944..1998094_POSITIVE 377 50 6 145 89 tfor:TF2774|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_2959719..2959869_POSITIVE 375 47 10 49 14 tfor:TF2793|3end_possible_L-lysine_2,3-aminomutase_2979888..2980038_POSITIVE 371 50 17 39 2 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 371 67 27 47 4 tfor:TF0445|3end_hypothetical_protein_467296..467446_POSITIVE 363 69 21 75 2 tfor:TF0248|3end_hypothetical_protein_278895..279045_POSITIVE 361 49 1 143 92 tfor:TF1553|3end_copper_homeostasis_protein_1659681..1659831_POSITIVE 360 49 18 73 37 tfor:TF2188|3end_conserved_hypothetical_protein_2363535..2363685_POSITIVE 360 68 27 102 41 tfor:TF0993|3end_hypothetical_protein_1048782..1048932_POSITIVE 356 46 2 56 16 tfor:TF3113|3end_hypothetical_protein_3349706..3349856_POSITIVE 356 45 4 52 10 tfor:TF0326|3end_hypothetical_protein_354317..354467_POSITIVE 355 67 21 61 16 tfor:TF0093|3end_outer_membrane_protein,_TonB_dependent_receptor_103618..103768_POSITIVE 353 66 22 140 92 tfor:TF1487|3end_orotate_phosphoribosyltransferase_1588522..1588672_POSITIVE 352 66 22 72 7 tfor:TF2642|3end_acetylornithine_deacetylase_2811827..2811977_POSITIVE 352 44 6 76 37 tfor:TF1218|3end_hypothetical_protein_1304383..1304533_POSITIVE