Origin IGS: taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcttacgaatcaagaagaagataagtaaattttctaacgcatgcaagcatttggacaactcactgttccccaaatgcttgctttttttataaagttaaaagtctgctaatttatgaaattacacaaatagcagtcaagtttta Mask Tandem Repeat Region ================================================ taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Find is-nt database================================================ Query_seq: TF3037:TF3038|TF3037:TF3038:galactokinase:conserved hypothetical protein:->->:3264327..3264470 144 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF3037:TF3038|TF3037:TF3038:galactokinase:conserved hypothetical protein:->->:3264327..3264470 144 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF3037:TF3038|TF3037:TF3038:galactokinase:conserved hypothetical protein:->->:3264327..3264470 144 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 3 End: 95 .. F K V A I Q T I E Y I L L S K V K Y F F C A N P S C H T T W M ................................................. taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 87 HP_score: -12.0 Tail_Score: -2.53392 Start: 62 End: 102 Full_Region: TAACTTTATAAAAAA AGCAAGCATTTGGGGAAC AGTGA GTTGTCCAAATGCTTGCA TGCGTTAGAAAATTT .............................................................AGCAAGCATTTGGGGAACAGTGAGTTGTCCAAATGCTTGCA.......................................... TransTerm Strand: - Conf: 100 HP_score: -13.3 Tail_Score: -5.63504 Start: 63 End: 101 Full_Region: aattttctaacgcat gcaagcatttggacaac tcact gttccccaaatgcttgc tttttttataaagtt ..............................................................gcaagcatttggacaactcactgttccccaaatgcttgc........................................... Find igs database================================================ Query_seq: TF3037:TF3038|TF3037:TF3038:galactokinase:conserved hypothetical protein:->->:3264327..3264470 144 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Intra-Species Hit: Count: 1 Min: 1 Max: 144 Len: 144 Subject: tfor_TF3037_TF3038|galactokinase:conserved hypothetical protein|POSITIVE:POSITIVE|[3264327,3264470]|144 HSP 1 e-value: 7.0E-64 bit: 244.0 Len: 144 Query Start:1 Query End:144 Subject Strand: POSITIVE Subject Start: 1 Subject End: 144 taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttatnnnnnnngcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag taaaacttgactgctatttgtgtaatttcataaattagcagacttttaactttataaaaaaagcaagcatttggggaacagtgagttgtccaaatgcttgcatgcgttagaaaatttacttatcttcttcttgattcgtaagag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACUUGACUGCUAUUUGUGUAAUUUCAUAAAUUAGCAGACUUUUAACUUUAUAAAAAAAGCAAGCAUUUGGGGAACAGUGAGUUGUCCAAAUGCUUGCAUGCGUUAGAAAAUUUACUUAUCUUCUUCUUGAUUCGUAAGAG ..........((((((((((((......)))))).))))))..(((((((.((....))...(((((((((((((.(((.....))).)))))))))))))....)))))))..(((((..(((........)))..))))).. (-42.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUACGAAUCAAGAAGAAGAUAAGUAAAUUUUCUAACGCAUGCAAGCAUUUGGACAACUCACUGUUCCCCAAAUGCUUGCUUUUUUUAUAAAGUUAAAAGUCUGCUAAUUUAUGAAAUUACACAAAUAGCAGUCAAGUUUUA ................(((((((......))))))).......((((((((((((..(((.....)))..))))))))))))...................(.((((((.(((.((......)).))))))))).)........ (-34.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 120 73 61 1 tfor:TF1009|5end_conserved_hypothetical_protein;_posible_rod-shape_determining_protein,_MreD_1064290..1064520_POSITIVE 320 110 75 187 148 tfor:TF1492|5end_ribonuclease_H-related_protein_1592017..1592247_POSITIVE 318 108 62 217 169 tfor:TF1524|5end_tryptophanyl-tRNA_synthetase_1630946..1631176_POSITIVE 312 108 67 141 88 tfor:TF0826|5end_primosomal_protein_N'_883751..883981_POSITIVE 307 108 67 181 144 tfor:TF1466|5end_DNA_gyrase_modulator_1555469..1555699_POSITIVE 289 108 61 199 146 tfor:TF0529|5end_transposase_554776..555006_POSITIVE 279 106 61 50 6 tfor:TF1521|5end_GTP-binding_protein_1628910..1629140_POSITIVE 278 106 62 74 11 tfor:TF1234|5end_rhamnulose-1-phosphate_aldolase_1318875..1319105_POSITIVE 277 99 65 111 78 tfor:TF2398|5end_magnesium_chelatase,_D/I_family_2569549..2569779_POSITIVE 275 102 62 151 108 tfor:TF1619|5end_riboflavin_synthase,_beta_subunit_1733948..1734178_POSITIVE 273 105 63 229 191 tfor:TF1293|5end_ATPase,_possible_AAA_superfamily_1374579..1374809_POSITIVE 273 108 61 197 146 tfor:TF0488|5end_xylose_isomerase_510137..510367_POSITIVE 272 108 63 186 143 tfor:TF2377|5end_orotidine_5'_phosphate_decarboxylase_2541799..2542029_POSITIVE 272 105 61 158 122 tfor:TF1045|5end_conserved_hypothetical_protein_1107665..1107895_POSITIVE 267 110 61 137 84 tfor:TF1808|5end_DNA_processing_protein_subunit_A_1949356..1949586_POSITIVE 265 110 63 215 166 tfor:TF2255|5end_transfer_region-related_protein,_TraH_2427504..2427734_POSITIVE 263 110 61 167 113 tfor:TF0729|5end_DNA_primase_780107..780337_POSITIVE 262 107 62 55 3 tfor:TF0919|5end_conserved_hypothetical_protein_957541..957771_POSITIVE 259 106 64 89 41 tfor:TF1891|5end_hypothetical_protein_2043389..2043619_POSITIVE 259 106 63 225 179 tfor:TF0730|5end_conserved_hypothetical_protein_782249..782479_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 321 120 73 65 5 tfor:TF1008|3end_rod_shape-determining_protein_MreC_1064374..1064524_POSITIVE 320 107 61 142 97 tfor:TF3141|3end_NADH:_ubiquinone_oxidoreductase,_subunit_A_3376267..3376417_POSITIVE 289 108 61 122 69 tfor:TF0528|3end_two-component_system_sensor_histidine_kinase_554779..554929_POSITIVE 286 106 67 144 107 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 284 107 63 128 86 tfor:TF1292|3end_sensor_histidine_protein_kinase_1374554..1374704_POSITIVE 277 99 65 51 18 tfor:TF2397|3end_magnesium_and_cobalt_transport_protein_2569569..2569719_POSITIVE 276 105 67 43 3 tfor:TF0692|3end_conserved_hypothetical_protein_736992..737142_POSITIVE 267 110 61 126 73 tfor:TF1807|3end_thioesterase_family_protein_1949425..1949575_POSITIVE 264 109 67 126 83 tfor:TF2346|3end_hypothetical_protein_2509973..2510123_POSITIVE 263 107 63 151 109 tfor:TF2375|3end_conserved_hypothetical_protein_2541845..2541995_POSITIVE 260 89 51 131 99 tfor:TF1507|3end_outer_membrane_receptor,_TonB-dependent_1616268..1616418_POSITIVE 254 106 62 95 53 tfor:TF1579|3end_hypothetical_protein_1687274..1687424_POSITIVE 253 108 63 70 17 tfor:TF1687|3end_conserved_hypothetical_protein;_possible_periplasmic_protein_1803459..1803609_POSITIVE 253 106 61 90 31 tfor:TF1167|3end_hypothetical_protein_1248932..1249082_POSITIVE 252 109 63 107 65 tfor:TF3026|3end_conserved_hypothetical_protein_3250254..3250404_POSITIVE 250 106 63 71 13 tfor:TF0002|3end_hypothetical_protein_647..797_POSITIVE 249 107 67 135 93 tfor:TF2693|3end_possible_aminotransferase_2869720..2869870_POSITIVE 249 100 58 149 106 tfor:TF1511|3end_outer_membrane_protein,_TonB_dependent_receptor_1624457..1624607_POSITIVE 248 108 66 65 6 tfor:TF2642|3end_acetylornithine_deacetylase_2811827..2811977_POSITIVE 247 109 70 90 55 tfor:TF2789|3end_enoyl-[acyl-carrier-protein]_reductase_2973365..2973515_POSITIVE