Origin IGS: ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcagtccggcatattagggcgtctaataagcacgtattaggatgcctaataaacacgtattagggcggcgcatacgtacctgtcaaattatgtcaggctttcagcccttgtgcccatgcgagctaaccccttcggggcgttgaggggtttaggtgttgaattgttggctgattgctgacagctgtaagagccctgaaagggcgtaataattcagcccaatgcgtagcgaagcgaagcgttgggaaaacagggcgaccccctcggcggagggctgaaagcctgacataattcaatggttaatggttaattgtcaattgttatgtgcccgaacgcagagggacatccccccctcccccccttcaaagggggaaagagcgaagcgcagcgcacgaagccttgacctttgggttacttttgggtcaagccaaaagtaacggaaagaacgctctgaacaataaaagaaacgaataacaaatagtgaataatgaaaa Mask Tandem Repeat Region ================================================ ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgccggactgatta Find is-nt database================================================ Query_seq: TF2998:TF2999|TF2998:TF2999:surface antigen BspA:conserved hypothetical protein:->->:3215212..3215705 494 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2998:TF2999|TF2998:TF2999:surface antigen BspA:conserved hypothetical protein:->->:3215212..3215705 494 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2998:TF2999|TF2998:TF2999:surface antigen BspA:conserved hypothetical protein:->->:3215212..3215705 494 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 23 End: 178 ...................... M R F F Y C S E R S F R Y F W L D P K V T Q R S R L R A L R F A L S P F E G G E G G M S L C V R A H N N ............................................................................................................................................................................................................................................................................................................................ Protein_Len: 46 Strand: + Start: 203 End: 340 .......................................................................................................................................................................................................... M M S G F Q P S A E G V A L F S Q R F A S L R I G L N Y Y A L S G L L Q L S A I S Q Q F N T .......................................................................................................................................................... Protein_Len: 56 Strand: + Start: 199 End: 366 ...................................................................................................................................................................................................... M N Y V R L S A L R R G G R P V F P T L R F A T H W A E L L R P F R A L T A V S N Q P T I Q H L N P S T P R R G ................................................................................................................................ Protein_Len: 40 Strand: + Start: 282 End: 401 ......................................................................................................................................................................................................................................................................................... M I T P F Q G S Y S C Q Q S A N N S T P K P L N A P K G L A R M G T R A E S L T ............................................................................................. Protein_Len: 35 Strand: - Start: 352 End: 456 ............................................................................................................................................................................................................................................................................................................................................................... R G S P T L E C P C L P Q F G S M I Q C T R I R R G L V H K N P M R M ...................................... Protein_Len: 39 Strand: - Start: 317 End: 433 ............................................................................................................................................................................................................................................................................................................................ C D A L L E V G L G R L A G F P N A R M P V L A S L R V Y N S L Y T H A A R M ............................................................. Protein_Len: 38 Strand: - Start: 207 End: 320 .............................................................................................................................................................................................................. T L S E A R R R P P R G T K G V S R K A V C Q A S N N R G K L A R V A T L M .............................................................................................................................................................................. Protein_Len: 30 Strand: - Start: 95 End: 184 .............................................................................................. P K T R Q A E S K G G K F P P L P P H G E A N P C M V I S M ...................................................................................................................................................................................................................................................................................................................... Protein_Len: 49 Strand: - Start: 61 End: 207 ............................................................ K Q S S G F T V W L D L S R A S R K A R E G K S P P S P P I D R Q T R A C L L Q C N V M L W Q I M ............................................................................................................................................................................................................................................................................................... Protein_Len: 54 Strand: - Start: 24 End: 185 ....................... E N R K N N L A N K G N S K A Q G L L L G F T L A E H A A S R E K G R Q L P P P P S T G R R E P V Y C N V M ..................................................................................................................................................................................................................................................................................................................... ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2998:TF2999|TF2998:TF2999:surface antigen BspA:conserved hypothetical protein:->->:3215212..3215705 494 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaagggggggagggggggatgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta Intra-Species Hit: Count: 16 Min: 1 Max: 494 Len: 494 Subject: tfor_TF2998_TF2999|surface antigen BspA:conserved hypothetical protein|POSITIVE:POSITIVE|[3215212,3215705]|494 HSP 1 e-value: 0.0 bit: 686.0 Len: 346 Query Start:149 Query End:494 Subject Strand: POSITIVE Subject Start: 149 Subject End: 494 ....................................................................................................................................................atgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta ....................................................................................................................................................atgtccctctgcgttcgggcacataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatgggcacaagggctgaaagcctgacataatttgacaggtacgtatgcgccgccctaatacgtgtttattaggcatcctaatacgtgcttattagacgccctaatatgccggactgatta HSP 2 e-value: 3.0E-69 bit: 264.0 Len: 133 Query Start:1 Query End:133 Subject Strand: POSITIVE Subject Start: 1 Subject End: 133 ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaa......................................................................................................................................................................................................................................................................................................................................................................... ttttcattattcactatttgttattcgtttcttttattgttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgctgcgcttcgctctttccccctttgaa......................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2438_TF2439|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2616218,2616519]|302 HSP 1 e-value: 2.0E-42 bit: 174.0 Len: 132 Query Start:186 Query End:317 Subject Strand: POSITIVE Subject Start: 95 Subject End: 225 .........................................................................................................................................................................................taaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcag................................................................................................................................................................................. .........................................................................................................................................................................................taacaattaacaattaaattatgtcaggctttcagccctccgctgagggggtcgcc-tgttttcccaacgcttcgcttcgctacacattgggctgaattaatgcgccctttcagggcttttacagcagtcag................................................................................................................................................................................. Subject: tfor_TF0018_TF0019|hypothetical protein:heat shock protein ClpB|POSITIVE:POSITIVE|[12152,12589]|438 HSP 1 e-value: 5.0E-40 bit: 167.0 Len: 166 Query Start:188 Query End:353 Subject Strand: POSITIVE Subject Start: 17 Subject End: 184 ...........................................................................................................................................................................................accattaaccattgaattatgtcaggctttcagccctccgccgagggggt-cgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagc-caacaattcaacacctaaacccctcaac............................................................................................................................................. ...........................................................................................................................................................................................accattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaac............................................................................................................................................. Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 1.0E-34 bit: 149.0 Len: 151 Query Start:170 Query End:320 Subject Strand: POSITIVE Subject Start: 731 Subject End: 881 .........................................................................................................................................................................cataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaa.............................................................................................................................................................................. .........................................................................................................................................................................cataacaattgacaattaaccattaacaattgaattacgtcaggcttacagccctccgccgagatgcgatgccttttttcccaacgcttcgctgcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaa.............................................................................................................................................................................. HSP 2 e-value: 9.0E-17 bit: 89.7 Len: 49 Query Start:60 Query End:108 Subject Strand: POSITIVE Subject Start: 216 Subject End: 264 ...........................................................acttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. ...........................................................acttttggcttgaccaaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 2.0E-33 bit: 145.0 Len: 112 Query Start:209 Query End:320 Subject Strand: POSITIVE Subject Start: 830 Subject End: 944 ................................................................................................................................................................................................................tcaggctttcagccctccgccgagggggtcgccctgttttc---ccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaa.............................................................................................................................................................................. ................................................................................................................................................................................................................tcaggctttcagccctccgctgagggggttgccctgttttttttccaacgcttcgcttcgctgcgcattgggctgaattaatacgccctttcagggctcttacaacggtcagcaa.............................................................................................................................................................................. HSP 2 e-value: 3.0E-29 bit: 131.0 Len: 102 Query Start:209 Query End:310 Subject Strand: POSITIVE Subject Start: 511 Subject End: 611 ................................................................................................................................................................................................................tcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacag........................................................................................................................................................................................ ................................................................................................................................................................................................................tcaggctttcagccctccgccgagggggtcgccctg-tttcccaacgcttcgcttcgctgcacattggactgaattaacgcgccctttcagggctgttacag........................................................................................................................................................................................ HSP 3 e-value: 2.0E-26 bit: 121.0 Len: 69 Query Start:40 Query End:108 Subject Strand: POSITIVE Subject Start: 195 Subject End: 263 .......................................ttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. .......................................ttcagagcgctctttctgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. HSP 4 e-value: 2.0E-17 bit: 91.7 Len: 46 Query Start:384 Query End:429 Subject Strand: POSITIVE Subject Start: 1097 Subject End: 1142 ...............................................................................................................................................................................................................................................................................................................................................................................................agggctgaaagcctgacataatttgacaggtacgtatgcgccgccc................................................................. ...............................................................................................................................................................................................................................................................................................................................................................................................agggctgaaagcctgacataatttgacaggtacgtatgcgccgccc................................................................. HSP 5 e-value: 2.0E-11 bit: 71.9 Len: 71 Query Start:326 Query End:396 Subject Strand: POSITIVE Subject Start: 967 Subject End: 1038 .....................................................................................................................................................................................................................................................................................................................................caacaattcaacacctaaacccctcaacgccccgaaggggttagctcgcatg-ggcacaagggctgaaagcc.................................................................................................. .....................................................................................................................................................................................................................................................................................................................................caacaatcgaacacctcaacacctcaacgccccgaaggggttagctcgcccgaggcccaagggctgaaagcc.................................................................................................. Subject: tfor_TF2500_TF2501|ABC transporter, ATP-binding protein:DNA mismatch repair protein|POSITIVE:POSITIVE|[2675363,2676419]|1057 HSP 1 e-value: 7.0E-33 bit: 143.0 Len: 140 Query Start:181 Query End:320 Subject Strand: NEGATIVE Subject Start: 195 Subject End: 332 ....................................................................................................................................................................................acaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaa.............................................................................................................................................................................. ....................................................................................................................................................................................acaattgacaattaacaactgaattacgtcaggctttcatccctccgccgaggggg-cgcc-tgttttcccaacgcttcgcttcgctctgcattgggctgaattaatgcgccctttcagggcttttacaacggtcagcaa.............................................................................................................................................................................. HSP 2 e-value: 9.0E-17 bit: 89.7 Len: 49 Query Start:60 Query End:108 Subject Strand: NEGATIVE Subject Start: 663 Subject End: 711 ...........................................................acttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. ...........................................................acttttggcttgacccaaaagtaaccaaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2914_TF2915|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3109704,3110561]|858 HSP 1 e-value: 6.0E-27 bit: 123.0 Len: 204 Query Start:170 Query End:373 Subject Strand: POSITIVE Subject Start: 319 Subject End: 509 .........................................................................................................................................................................cataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcagccaacaattcaacacctaaacccctcaacgccccgaaggggttagctcg......................................................................................................................... .........................................................................................................................................................................cataaccattaaccattgacaattaacaactgaattacgtcaggctttcagccctccgccgaggggggtgcc-tgttttcccaacgcttcgctgcac-----attgggctgaattaacgtgccctttcagggcttttacagcggtcagca-------aacacctcaacacctcaacacctcaacgccccgaaggggttagctcg......................................................................................................................... HSP 2 e-value: 1.0E-12 bit: 75.8 Len: 46 Query Start:60 Query End:105 Subject Strand: POSITIVE Subject Start: 21 Subject End: 66 ...........................................................acttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgc..................................................................................................................................................................................................................................................................................................................................................................................................... ...........................................................acttttggcttgacccaaaagtaaccaaaaagtcaaggcttcgtgc..................................................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF1848_TF1850|conserved hypothetical protein:Na+/H+-exchanging protein|POSITIVE:POSITIVE|[1998005,1998681]|677 HSP 1 e-value: 6.0E-27 bit: 123.0 Len: 137 Query Start:170 Query End:306 Subject Strand: NEGATIVE Subject Start: 75 Subject End: 209 .........................................................................................................................................................................cataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctctt............................................................................................................................................................................................ .........................................................................................................................................................................cataacaattaaccattgacaattaacaattgaattacgtcaggctttcagccctccgctgagggggtcg--ctctttccccaacacttcgctgcgctccacattgggctgaattaacgcgccctttcagggctctt............................................................................................................................................................................................ Subject: tfor_TF2922_TF2923|surface antigen BspA:hypothetical protein|POSITIVE:POSITIVE|[3121599,3122307]|709 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 69 Query Start:40 Query End:108 Subject Strand: POSITIVE Subject Start: 139 Subject End: 207 .......................................ttcagagcgttctttccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. .......................................ttcagagcgctctttccgttacttttggcttgacccaaaagtaaccaaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-16 bit: 87.7 Len: 56 Query Start:209 Query End:264 Subject Strand: POSITIVE Subject Start: 20 Subject End: 75 ................................................................................................................................................................................................................tcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttc...................................................................................................................................................................................................................................... ................................................................................................................................................................................................................tcaggctttcagccctccgccgaggggggcgccctttttccccaacgcttcgcttc...................................................................................................................................................................................................................................... Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 2.0E-24 bit: 115.0 Len: 113 Query Start:199 Query End:311 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 ......................................................................................................................................................................................................ttgaattatgtcaggctttcagccctccgccgagggggtcgc---cctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagc....................................................................................................................................................................................... ......................................................................................................................................................................................................ttgaattacgtcaggctttcagccctccgccgagggggagggacgccttttttcccaacgcttcgcttcgctgcacattgggctgaattaacgcgccctttcagggcttttacagc....................................................................................................................................................................................... HSP 2 e-value: 8.0E-14 bit: 79.8 Len: 71 Query Start:333 Query End:403 Subject Strand: POSITIVE Subject Start: 180 Subject End: 251 ............................................................................................................................................................................................................................................................................................................................................tcaacacctaaacccctcaacgccccgaaggggttagctcgcatg-ggcacaagggctgaaagcctgacata........................................................................................... ............................................................................................................................................................................................................................................................................................................................................tcaacacctcaacacctcaacgccccgaaggggttagctcgcccgaggcccaagggctgaaagcccgacata........................................................................................... Subject: tfor_TF2815_TF2816|meso-diaminopimelate D-dehydrogenase:hypothetical protein|POSITIVE:POSITIVE|[3006481,3007064]|584 HSP 1 e-value: 9.0E-20 bit: 99.6 Len: 78 Query Start:241 Query End:318 Subject Strand: POSITIVE Subject Start: 228 Subject End: 305 ................................................................................................................................................................................................................................................cctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagc................................................................................................................................................................................ ................................................................................................................................................................................................................................................cctgttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggcttttaaagcagtcagc................................................................................................................................................................................ HSP 2 e-value: 3.0E-16 bit: 87.7 Len: 56 Query Start:170 Query End:225 Subject Strand: NEGATIVE Subject Start: 464 Subject End: 519 .........................................................................................................................................................................cataacaattgacaattaaccattaaccattgaattatgtcaggctttcagccctc............................................................................................................................................................................................................................................................................. .........................................................................................................................................................................cataacaattaacaattgaccattaacaattgaattatgtcaggctttcagccctc............................................................................................................................................................................................................................................................................. Subject: tfor_TF2477_TF2478|phosphatase:hypothetical protein|POSITIVE:POSITIVE|[2655026,2655514]|489 HSP 1 e-value: 6.0E-18 bit: 93.7 Len: 102 Query Start:202 Query End:303 Subject Strand: NEGATIVE Subject Start: 362 Subject End: 456 .........................................................................................................................................................................................................aattatgtcaggctttcagccctccgccgagggggtcgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggct............................................................................................................................................................................................... .........................................................................................................................................................................................................aattatgtcgggctttcagccctccgccgaggagattgcc--tttttcccaa-----cgcttcgctacgcattgggctgaattaatacgccctttcagggct............................................................................................................................................................................................... Subject: tfor_TF0598_TF0599|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[629561,629974]|414 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 55 Query Start:54 Query End:108 Subject Strand: NEGATIVE Subject Start: 20 Subject End: 74 .....................................................tccgttacttttggcttgacccaaaagtaacccaaaggtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. .....................................................tccgtcacttttggcttgacccaaaagtaaccaaaaagtcaaggcttcgtgcgct.................................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 8.0E-8 bit: 60.0 Len: 30 Query Start:378 Query End:407 Subject Strand: NEGATIVE Subject Start: 90 Subject End: 119 .........................................................................................................................................................................................................................................................................................................................................................................................ggcacaagggctgaaagcctgacataattt....................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................ggcacaagggctgaaagcctgacataattt....................................................................................... Subject: tfor_TF0827_TF0828|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[886980,887229]|250 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 48 Query Start:264 Query End:311 Subject Strand: NEGATIVE Subject Start: 135 Subject End: 182 .......................................................................................................................................................................................................................................................................cgctacgcattgggctgaattattacgccctttcagggctcttacagc....................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................cgcttcgcattgggctgaattattacgccctttcagggcttttacagc....................................................................................................................................................................................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:191 Query End:226 Subject Strand: NEGATIVE Subject Start: 209 Subject End: 244 ..............................................................................................................................................................................................attaaccattgaattatgtcaggctttcagccctcc............................................................................................................................................................................................................................................................................ ..............................................................................................................................................................................................attaacaattgaattacgtcaggctttcagccctcc............................................................................................................................................................................................................................................................................ Subject: tfor_TF1181_TF1183|methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1268052,1268530]|479 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 57 Query Start:343 Query End:399 Subject Strand: NEGATIVE Subject Start: 212 Subject End: 269 ......................................................................................................................................................................................................................................................................................................................................................aacccctcaacgccccgaaggggttagctcgcatgg-gcacaagggctgaaagcctga............................................................................................... ......................................................................................................................................................................................................................................................................................................................................................aacccctcaacgccccgaaggggttagcccgcacggagcacaagggctgaaggcctga............................................................................................... HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 47 Query Start:264 Query End:310 Subject Strand: NEGATIVE Subject Start: 375 Subject End: 421 .......................................................................................................................................................................................................................................................................cgctacgcattgggctgaattattacgccctttcagggctcttacag........................................................................................................................................................................................ .......................................................................................................................................................................................................................................................................cgcttcgcattgggctgaattaatgcgccctttcagggcttttacag........................................................................................................................................................................................ Subject: tfor_TF0958_TF0959|conserved hypothetical protein:periplasmic protease|POSITIVE:POSITIVE|[1004970,1005357]|388 HSP 1 e-value: 5.0E-9 bit: 63.9 Len: 80 Query Start:245 Query End:324 Subject Strand: POSITIVE Subject Start: 31 Subject End: 110 ....................................................................................................................................................................................................................................................ttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggctcttacagctgtcagcaatcag.......................................................................................................................................................................... ....................................................................................................................................................................................................................................................ttttcccaacgctttgcttcgctctacattgggctgaattaacgtgccctttcagggcttttacagcgttcagtaatcag.......................................................................................................................................................................... HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 70 Query Start:333 Query End:402 Subject Strand: POSITIVE Subject Start: 134 Subject End: 204 ............................................................................................................................................................................................................................................................................................................................................tcaacacctaaacccctcaacgccccgaaggggttagctcgc-atgggcacaagggctgaaagcctgacat............................................................................................ ............................................................................................................................................................................................................................................................................................................................................tcaacaaccaaacacctcaacgccacgaaagggttagctcgcaaggggtacaagggctgtaagcctgacat............................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCAUUAUUCACUAUUUGUUAUUCGUUUCUUUUAUUGUUCAGAGCGUUCUUUCCGUUACUUUUGGCUUGACCCAAAAGUAACCCAAAGGUCAAGGCUUCGUGCGCUGCGCUUCGCUCUUUCCCCCUUUGAAGGGGGGGAGGGGGGGAUGUCCCUCUGCGUUCGGGCACAUAACAAUUGACAAUUAACCAUUAACCAUUGAAUUAUGUCAGGCUUUCAGCCCUCCGCCGAGGGGGUCGCCCUGUUUUCCCAACGCUUCGCUUCGCUACGCAUUGGGCUGAAUUAUUACGCCCUUUCAGGGCUCUUACAGCUGUCAGCAAUCAGCCAACAAUUCAACACCUAAACCCCUCAACGCCCCGAAGGGGUUAGCUCGCAUGGGCACAAGGGCUGAAAGCCUGACAUAAUUUGACAGGUACGUAUGCGCCGCCCUAAUACGUGUUUAUUAGGCAUCCUAAUACGUGCUUAUUAGACGCCCUAAUAUGCCGGACUGAUUA ...(((...........((((((...(((..............((((.((((((..(((((((((((......)))))))))))..)))))...).)))).((((.(.((((....((((..((((((....))))))..)))).(((....)))...))))..).))))..)))...))))))....((........(..(((((((((((((((((((((((.....((((((...((((((...............((........))...(((((((....))).))))...)))))).......((((........))))...................))))))...(((((...)))))..((((....))))...)))))))))))))))))))))))..)..(((((((((.......(((((((......))))))).......)))))))))((((((.....))))))....))..)))... (-175.74) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCAGUCCGGCAUAUUAGGGCGUCUAAUAAGCACGUAUUAGGAUGCCUAAUAAACACGUAUUAGGGCGGCGCAUACGUACCUGUCAAAUUAUGUCAGGCUUUCAGCCCUUGUGCCCAUGCGAGCUAACCCCUUCGGGGCGUUGAGGGGUUUAGGUGUUGAAUUGUUGGCUGAUUGCUGACAGCUGUAAGAGCCCUGAAAGGGCGUAAUAAUUCAGCCCAAUGCGUAGCGAAGCGAAGCGUUGGGAAAACAGGGCGACCCCCUCGGCGGAGGGCUGAAAGCCUGACAUAAUUCAAUGGUUAAUGGUUAAUUGUCAAUUGUUAUGUGCCCGAACGCAGAGGGACAUCCCCCCCUCCCCCCCUUCAAAGGGGGAAAGAGCGAAGCGCAGCGCACGAAGCCUUGACCUUUGGGUUACUUUUGGGUCAAGCCAAAAGUAACGGAAAGAACGCUCUGAACAAUAAAAGAAACGAAUAACAAAUAGUGAAUAAUGAAAA ...((((..((.....(((((((.(((((((......(((((((...)))))))......))))))).((((((...((((((.((..((((((((((((((((((((((((.((((.........(((((((....))).)))).((((((....(((((((((((((((((........)))))......((((.....))))..))))))))..((((((((...((...))...))))))))..))))....))))))...))))))))))))))))))))))))))))..)))))..)))..................(((((.....(((.(((((........))))).((((((....)))))).....)))..))))))))).))..))))))).((((..(((((((((((......)))))))))))..))))..))..))))........................................ (-186.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1074 160 111 62 11 tfor:TF1842|5end_hypothetical_protein_1992571..1992801_POSITIVE 991 159 111 54 5 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 981 160 111 220 171 tfor:TF0540|5end_conserved_hypothetical_protein;_possible_surface_protein_567286..567516_POSITIVE 963 166 121 165 118 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 963 166 121 61 14 tfor:TF3106|5end_hypothetical_protein_3338355..3338585_POSITIVE 935 108 61 211 164 tfor:TF1660|5end_hypothetical_protein_1775269..1775499_POSITIVE 886 108 61 229 182 tfor:TF1843|5end_surface_antigen_BspA_1992834..1993064_POSITIVE 879 260 212 181 135 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 859 108 61 210 163 tfor:TF1846|5end_hypothetical_protein_1996726..1996956_POSITIVE 859 108 61 104 57 tfor:TF1845|5end_hypothetical_protein_1996620..1996850_POSITIVE 856 160 111 55 1 tfor:TF2746|5end_hypothetical_protein_2927996..2928226_POSITIVE 854 168 121 66 12 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 835 169 121 217 169 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 817 108 61 87 39 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 771 300 251 113 63 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 771 300 251 61 11 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 754 160 111 190 147 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 753 285 245 93 53 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 748 318 271 231 184 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 744 310 264 200 154 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1016 160 111 123 73 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 961 130 83 137 90 tfor:TF1842|3end_hypothetical_protein_1992800..1992950_POSITIVE 945 160 111 102 54 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 941 440 392 59 11 tfor:TF1839|3end_conserved_hypothetical_protein_1989699..1989849_POSITIVE 935 108 61 71 24 tfor:TF1659|3end_hypothetical_protein_1775209..1775359_POSITIVE 872 110 61 142 91 tfor:TF2817|3end_hypothetical_protein_3007626..3007776_POSITIVE 859 108 61 97 50 tfor:TF1846|3end_hypothetical_protein_1997015..1997165_POSITIVE 859 108 61 87 40 tfor:TF1845|3end_hypothetical_protein_1997005..1997155_POSITIVE 859 108 61 97 50 tfor:TF1844|3end_hypothetical_protein_1996693..1996843_POSITIVE 859 108 61 71 24 tfor:TF1187|3end_hypothetical_protein_1269872..1270022_POSITIVE 856 108 61 87 40 tfor:TF0538|3end_hypothetical_protein_567135..567285_POSITIVE 849 110 61 57 6 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 845 160 111 81 28 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 830 108 61 97 50 tfor:TF3105|3end_hypothetical_protein_3338342..3338492_POSITIVE 830 108 61 71 24 tfor:TF0598|3end_hypothetical_protein_629500..629650_POSITIVE 774 110 61 101 50 tfor:TF1183|3end_hypothetical_protein_1268728..1268878_POSITIVE 765 319 271 86 38 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 759 108 66 43 1 tfor:TF2795|3end_hypothetical_protein_2980909..2981059_POSITIVE 744 310 264 115 69 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 736 110 61 101 50 tfor:TF1184|3end_hypothetical_protein_1269311..1269461_POSITIVE