Origin IGS: taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagtccatcctctttggggaagaaaatgcaaaagtaaatattatccttattatatcatccccaaaacaaaattaattcacgttttgttcgcccctatattcttaaaccatcaaacaaaaaacaatttaattcaggctttcagccctctgccgagagggtaccttttttccctacgcttcccttcgctacccatcgggctgaattaacacaccctttcagggctgtttttta Mask Tandem Repeat Region ================================================ taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Find is-nt database================================================ Query_seq: TF2840:TF2841|TF2840:TF2841:hypothetical protein:conserved hypothetical protein:->->:3031350..3031580 231 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2840:TF2841|TF2840:TF2841:hypothetical protein:conserved hypothetical protein:->->:3031350..3031580 231 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2840:TF2841|TF2840:TF2841:hypothetical protein:conserved hypothetical protein:->->:3031350..3031580 231 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: - Start: 29 End: 178 ............................ N L G I P L S P F R L S F L Y G R P L P S F A Q I L N N K T Q H N L F I P A F L V H I L K T K P I M ..................................................... Protein_Len: 60 Strand: - Start: 3 End: 194 .. F P H T L E A R H T A F P L T P F F P V R E A S P Q F G S N F Q K K N S P K L I Y P R V F R S N I K N Q P H Y L L S L M ..................................... taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2840:TF2841|TF2840:TF2841:hypothetical protein:conserved hypothetical protein:->->:3031350..3031580 231 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Intra-Species Hit: Count: 3 Min: 1 Max: 231 Len: 231 Subject: tfor_TF2840_TF2841|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[3031350,3031580]|231 HSP 1 e-value: 1.0E-128 bit: 458.0 Len: 231 Query Start:1 Query End:231 Subject Strand: POSITIVE Subject Start: 1 Subject End: 231 taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt taaaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaaggtaccctctcggcagagggctgaaagcctgaattaaattgttttttgtttgatggtttaagaatataggggcgaacaaaacgtgaattaattttgttttggggatgatataataaggataatatttacttttgcattttcttccccaaagaggatggactt Subject: tfor_TF1841_TF1842|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1992328,1992710]|383 HSP 1 e-value: 6.0E-19 bit: 95.6 Len: 101 Query Start:3 Query End:103 Subject Strand: POSITIVE Subject Start: 157 Subject End: 259 ..aaaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaagg--taccctctcggcagagggctgaaagcctgaat................................................................................................................................ ..aaaaacagccctgaaagggcgtattaattcagcccaatgtgcagcaaagcgaagcgttgggaaaaaagggcgaccccctcggcggagggctgaaagcctgaat................................................................................................................................ Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 4.0E-17 bit: 89.7 Len: 100 Query Start:4 Query End:103 Subject Strand: NEGATIVE Subject Start: 279 Subject End: 379 ...aaaacagccctgaaagggtgtgttaattcagcccgatgggtagcgaagggaagcgtagggaaaaaagg-taccctctcggcagagggctgaaagcctgaat................................................................................................................................ ...aaaacagccctgaaagggcgtaataattcagcccaatgcgtagcgaagcgaagcgttgggaaacagggcaaccccctcggcggagggctgaaagcctgaat................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAACAGCCCUGAAAGGGUGUGUUAAUUCAGCCCGAUGGGUAGCGAAGGGAAGCGUAGGGAAAAAAGGUACCCUCUCGGCAGAGGGCUGAAAGCCUGAAUUAAAUUGUUUUUUGUUUGAUGGUUUAAGAAUAUAGGGGCGAACAAAACGUGAAUUAAUUUUGUUUUGGGGAUGAUAUAAUAAGGAUAAUAUUUACUUUUGCAUUUUCUUCCCCAAAGAGGAUGGACUU .....((((((((.....)))).))))..((((.(((..((((....((((..(((((.(((...........((((((....))))))((.(((((..(((((((.(..(.(((((((((...((........)).....))))))))).)..).))))))).))))).)).........................))).))).))..))))))))..).)).))))... (-55.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUCCAUCCUCUUUGGGGAAGAAAAUGCAAAAGUAAAUAUUAUCCUUAUUAUAUCAUCCCCAAAACAAAAUUAAUUCACGUUUUGUUCGCCCCUAUAUUCUUAAACCAUCAAACAAAAAACAAUUUAAUUCAGGCUUUCAGCCCUCUGCCGAGAGGGUACCUUUUUUCCCUACGCUUCCCUUCGCUACCCAUCGGGCUGAAUUAACACACCCUUUCAGGGCUGUUUUUUA .............(((((((.((...(((....)))((((.......))))...)).)))))))((((((((........))))))))...........................((((((((..(((((((((.((....(((((((....)))))))...............((........))........)).)))))))))....(((.....))).)))))))). (-45.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 740 59 11 221 173 tfor:TF2172|5end_hypothetical_protein_2345822..2346052_POSITIVE 740 59 11 215 167 tfor:TF2173|5end_hypothetical_protein_2345816..2346046_POSITIVE 733 57 11 231 185 tfor:TF2439|5end_conserved_hypothetical_protein_2616380..2616610_POSITIVE 719 50 4 224 178 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 703 59 11 220 167 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 702 48 9 228 189 tfor:TF1175|5end_membrane-fusion_protein_1258140..1258370_POSITIVE 690 48 9 226 187 tfor:TF2351|5end_acetate_kinase_2515480..2515710_POSITIVE 686 57 11 217 167 tfor:TF0346|5end_hypothetical_protein_367048..367278_POSITIVE 676 59 13 215 169 tfor:TF0366|5end_hypothetical_protein_383564..383794_POSITIVE 676 59 13 148 102 tfor:TF0350|5end_hypothetical_protein_370794..371024_POSITIVE 662 70 21 226 177 tfor:TF2171|5end_hypothetical_protein_2345485..2345715_POSITIVE 653 59 13 215 169 tfor:TF0362|5end_hypothetical_protein_379952..380182_POSITIVE 641 100 53 223 174 tfor:TF2641|5end_riboflavin_kinase/FAD_synthase_2809681..2809911_POSITIVE 622 50 2 142 94 tfor:TF2127|5end_hypothetical_protein_2304038..2304268_POSITIVE 598 100 53 159 110 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 584 90 41 78 25 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 516 39 5 231 197 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 499 100 53 186 138 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 496 100 51 59 5 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 492 59 13 220 169 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 764 100 53 79 27 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 756 100 53 52 2 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 748 57 11 126 79 tfor:TF1592|3end_hypothetical_protein_1708557..1708707_POSITIVE 747 100 51 70 15 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 740 59 11 111 63 tfor:TF2171|3end_hypothetical_protein_2345792..2345942_POSITIVE 731 50 9 149 108 tfor:TF1588|3end_hypothetical_protein_1702560..1702710_POSITIVE 717 100 53 82 32 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE 717 100 53 75 25 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 693 59 11 52 4 tfor:TF0958|3end_conserved_hypothetical_protein_1004909..1005059_POSITIVE 687 55 11 123 79 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 686 57 11 52 2 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 605 48 3 109 64 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 541 57 19 43 1 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 535 100 56 47 1 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 525 48 3 63 18 tfor:TF2342|3end_glutathione_peroxidase_2506639..2506789_POSITIVE 419 126 82 63 18 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE 418 120 75 61 17 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 407 75 31 47 1 tfor:TF2126|3end_S-adenosylhomocysteine_hydrolase_2303996..2304146_POSITIVE 400 126 82 67 22 tfor:TF0343|3end_hypothetical_protein_366923..367073_POSITIVE 391 120 75 46 2 tfor:TF0819|3end_phosphoglycerate_mutase_879729..879879_POSITIVE