Origin IGS: tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tggttattttctaaatacatgattcggcgtgtaccacgcataataattgaccattgacaattaacaattaatttgtcattcagagcaaagcgaagaatctcctttccttttcctcgcgggattcctcactgtgttcggaatgacaaagggggttatttgctttcattcgcgttatttgcgttatttgcgttcggataaccctgcataacaattaacaattgaccattaacaattgaattatgtcaggctttca Mask Tandem Repeat Region ================================================ tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Find is-nt database================================================ Query_seq: TF2816:TF2817|TF2816:TF2817:hypothetical protein:hypothetical protein:->->:3007245..3007497 253 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2816:TF2817|TF2816:TF2817:hypothetical protein:hypothetical protein:->->:3007245..3007497 253 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2816:TF2817|TF2816:TF2817:hypothetical protein:hypothetical protein:->->:3007245..3007497 253 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 43 End: 171 .......................................... M L C R V I R T Q I T Q I T R M K A N N P L C H S E H S E E S R E E K E R R F F A L L .................................................................................. Protein_Len: 40 Strand: + Start: 132 End: 251 ................................................................................................................................... M P R G K G K E I L R F A L N D K L I V N C Q W S I I M R G T R R I M Y L E N N .. Protein_Len: 43 Strand: - Start: 59 End: 187 .......................................................... G F A F L A F L A F S L L Y G G K D N R V C H P I G R P F P F S I R R K A R F S L N M .................................................................. Protein_Len: 45 Strand: - Start: 49 End: 183 ................................................ A P N D S R L Y R L Y R S H F C I V G K T M G F V T L F G A L F L F P S E E S Q E S H C M ...................................................................... tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2816:TF2817|TF2816:TF2817:hypothetical protein:hypothetical protein:->->:3007245..3007497 253 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Intra-Species Hit: Count: 17 Min: 1 Max: 253 Len: 253 Subject: tfor_TF2816_TF2817|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3007245,3007497]|253 HSP 1 e-value: 1.0E-141 bit: 502.0 Len: 253 Query Start:1 Query End:253 Subject Strand: POSITIVE Subject Start: 1 Subject End: 253 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca Subject: tfor_TF2922_TF2923|surface antigen BspA:hypothetical protein|POSITIVE:POSITIVE|[3121599,3122307]|709 HSP 1 e-value: 3.0E-49 bit: 196.0 Len: 174 Query Start:24 Query End:197 Subject Strand: NEGATIVE Subject Start: 405 Subject End: 578 .......................gttaatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctt-tgctctgaatgacaaattaattgttaattgt........................................................ .......................gttaattgtcaatggttaattgttatgccgggttatccgaacgcaaatttcgcaaatgacgcgaatggacgcaaataaccccctttgtcattccgaacgtagtgaggaatcccgtggggaagaggaaa-gagattcttcgcttacgctctgaatgacaaattaatggttaattgt........................................................ HSP 2 e-value: 3.0E-18 bit: 93.7 Len: 55 Query Start:199 Query End:253 Subject Strand: NEGATIVE Subject Start: 363 Subject End: 417 ......................................................................................................................................................................................................aatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca ......................................................................................................................................................................................................aatggttaattgttatgcgtggtacacgccgaatcatgtatttagaaaataacca HSP 3 e-value: 4.0E-14 bit: 79.8 Len: 64 Query Start:153 Query End:216 Subject Strand: NEGATIVE Subject Start: 551 Subject End: 614 ........................................................................................................................................................gagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgc..................................... ........................................................................................................................................................gagattcatcgcttcgcgctgaatgacaaattaatggttaattgtcaatggttaattgttatgc..................................... Subject: tfor_TF1838_TF1839|tRNA/rRNA methyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1988806,1989486]|681 HSP 1 e-value: 1.0E-48 bit: 194.0 Len: 157 Query Start:97 Query End:253 Subject Strand: POSITIVE Subject Start: 96 Subject End: 252 ................................................................................................aataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca ................................................................................................aataaccgcctttgtcattccgaacaccgtgaggaatcccgcggagtaaaagaaag-agattcttcgcttacgctctgaatgacaaattaattgttaattgtcaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataacca Subject: tfor_TF0603_TF0604|glycosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[634899,635524]|626 HSP 1 e-value: 2.0E-34 bit: 147.0 Len: 126 Query Start:72 Query End:197 Subject Strand: POSITIVE Subject Start: 109 Subject End: 233 .......................................................................aacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgt........................................................ .......................................................................aacgcagatgacgcaaatggacgcaaataaccccctttgtcattccgaacaccgtgaggaatcccgcggggggaa-gaaaagagattcttcgcttcgctctgaatgacaaattaattgttaattgt........................................................ HSP 2 e-value: 4.0E-14 bit: 79.8 Len: 52 Query Start:175 Query End:226 Subject Strand: POSITIVE Subject Start: 50 Subject End: 101 ..............................................................................................................................................................................atgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacg........................... ..............................................................................................................................................................................atgacaaattaattgttaatggtcaattgtcaattgttatgcgtggtacacg........................... HSP 3 e-value: 6.0E-7 bit: 56.0 Len: 32 Query Start:20 Query End:51 Subject Strand: POSITIVE Subject Start: 60 Subject End: 91 ...................aattgttaatggtcaattgttaattgttatgc.......................................................................................................................................................................................................... ...................aattgttaatggtcaattgtcaattgttatgc.......................................................................................................................................................................................................... Subject: tfor_TF2823_TF2824|TPR-containing protein:nucleoside-transporting protein nupG|POSITIVE:POSITIVE|[3014398,3014840]|443 HSP 1 e-value: 2.0E-28 bit: 127.0 Len: 96 Query Start:104 Query End:199 Subject Strand: POSITIVE Subject Start: 101 Subject End: 195 .......................................................................................................ccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtca...................................................... .......................................................................................................ccctttgtcattccgaacgcagtgaggaatctcgcggggaaaagggaag-agattcttcgcttcgctctgaatgacaaattaatggttaattgtca...................................................... HSP 2 e-value: 2.0E-19 bit: 97.6 Len: 77 Query Start:26 Query End:102 Subject Strand: POSITIVE Subject Start: 1 Subject End: 77 .........................taatggtcaattgttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataac....................................................................................................................................................... .........................taatggttaattgtcaattgttatgccgggttatccgaacgcaaatttcgcaaataacgcgaatcaacgcaaataac....................................................................................................................................................... Subject: tfor_TF1841_TF1842|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1992328,1992710]|383 HSP 1 e-value: 8.0E-28 bit: 125.0 Len: 106 Query Start:99 Query End:204 Subject Strand: POSITIVE Subject Start: 1 Subject End: 106 ..................................................................................................taaccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctt-tgctctgaatgacaaattaattgttaattgtcaatggt................................................. ..................................................................................................taaccgcctctgtcattccgaacgcagtgaggaatctcgcgaggaagaggaaa-gagattcttcgcttacgctctgaatgacaaagtaattgttaattgtcaatggt................................................. HSP 2 e-value: 3.0E-24 bit: 113.0 Len: 103 Query Start:102 Query End:204 Subject Strand: POSITIVE Subject Start: 277 Subject End: 380 .....................................................................................................ccccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctt-tgctctgaatgac-aaattaattgttaattgtcaatggt................................................. .....................................................................................................ccccctttgtcattccgaacgcagtgaggaatctcgcgaggaagaggaaa-gagattcttcgcttacgctctgaatgacaaaagtaattgtcaattgtcaatggt................................................. HSP 3 e-value: 6.0E-7 bit: 56.0 Len: 36 Query Start:204 Query End:239 Subject Strand: NEGATIVE Subject Start: 122 Subject End: 157 ...........................................................................................................................................................................................................tcaattattatgcgtggtacacgccgaatcatgtat.............. ...........................................................................................................................................................................................................tcaattgttatgcgtggtacacgccaaatcatgtat.............. Subject: tfor_TF1578_TF1579|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1686477,1687028]|552 HSP 1 e-value: 7.0E-25 bit: 115.0 Len: 127 Query Start:108 Query End:234 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 121 ...........................................................................................................ttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctt-tgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatca................... ...........................................................................................................ttgtcattccgaacgcagtgaggaatctcgcggggtaaaggaaaagagattcttcgcttacgctctgaatgacaaattaatggttaatt-------gttaattgttatgcgtggtacacgccgaatca................... HSP 2 e-value: 3.0E-21 bit: 103.0 Len: 119 Query Start:108 Query End:226 Subject Strand: NEGATIVE Subject Start: 402 Subject End: 521 ...........................................................................................................ttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacg........................... ...........................................................................................................ttgtcattccgaacgcagtgaggaatctcgcggggtaaaagaaaagagattcatcgctcacgctctgaatgacatattaattgttaatggttaattgttaattgttatgcgtggtacacg........................... HSP 3 e-value: 2.0E-9 bit: 63.9 Len: 75 Query Start:49 Query End:123 Subject Strand: NEGATIVE Subject Start: 150 Subject End: 225 ................................................tgcagggttatccgaacgcaaataacgcaaat-aacgcgaatgaaagcaaataaccccctttgtcattccgaacac.................................................................................................................................. ................................................tgcagggttatccgaacgcaaatttcgcaaatgggcgcgaatcaacgcaaataacagtctttgtcattccgaacac.................................................................................................................................. HSP 4 e-value: 6.0E-7 bit: 56.0 Len: 32 Query Start:20 Query End:51 Subject Strand: NEGATIVE Subject Start: 412 Subject End: 443 ...................aattgttaatggtcaattgttaattgttatgc.......................................................................................................................................................................................................... ...................aattgttaatggttaattgttaattgttatgc.......................................................................................................................................................................................................... Subject: tfor_TF2997_TF2998|hypothetical protein:surface antigen BspA|POSITIVE:POSITIVE|[3211725,3211950]|226 HSP 1 e-value: 5.0E-23 bit: 109.0 Len: 102 Query Start:105 Query End:206 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 100 ........................................................................................................cctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtca............................................... ........................................................................................................cctttgtcattccgaacgtagtgaggaatcccgtggggaaaag--aatgagattcttcgcttcgctctgaatgacaaattaatggttaattgttaattgtca............................................... Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 3.0E-21 bit: 103.0 Len: 111 Query Start:106 Query End:216 Subject Strand: POSITIVE Subject Start: 223 Subject End: 333 .........................................................................................................ctttgtcattccgaacacagtgaggaatcccgcga-ggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaatggtcaattattatgc..................................... .........................................................................................................ctttgtcattccgaacgtagtgaggaatcccgccccggtaaagggaag-agattcttcgcttcgctctgaatgacaaattaatggttaattgtcaattgttaattgttatgc..................................... Subject: tfor_TF2914_TF2915|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3109704,3110561]|858 HSP 1 e-value: 4.0E-20 bit: 99.6 Len: 98 Query Start:104 Query End:201 Subject Strand: NEGATIVE Subject Start: 197 Subject End: 293 .......................................................................................................ccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgtcaat.................................................... .......................................................................................................ccctttgtcattccgaacaccgtgaggaatctcgcctcgaaaagggaaa-agattcttcgcttcgctctgaatgacaaattaatggttaattgacaat.................................................... HSP 2 e-value: 2.0E-9 bit: 63.9 Len: 56 Query Start:1 Query End:56 Subject Strand: NEGATIVE Subject Start: 313 Subject End: 368 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggt..................................................................................................................................................................................................... tgaaagcctgacgtaattcagttgttaattgtcaatggttaatggttatgccgggt..................................................................................................................................................................................................... Subject: tfor_TF2993_TF2994|SAM-dependent methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[3210478,3210744]|267 HSP 1 e-value: 2.0E-19 bit: 97.6 Len: 57 Query Start:182 Query End:238 Subject Strand: NEGATIVE Subject Start: 211 Subject End: 267 .....................................................................................................................................................................................attaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgta............... .....................................................................................................................................................................................attaattgttaattgtcaatggttaattgttatgcgtggtacacgccgaatcatgta............... HSP 2 e-value: 6.0E-7 bit: 56.0 Len: 36 Query Start:11 Query End:46 Subject Strand: POSITIVE Subject Start: 166 Subject End: 201 ..........acataattcaattgttaatggtcaattgttaattgt............................................................................................................................................................................................................... ..........acataattcaattgttaatggttaattgtcaattgt............................................................................................................................................................................................................... Subject: tfor_TF1848_TF1850|conserved hypothetical protein:Na+/H+-exchanging protein|POSITIVE:POSITIVE|[1998005,1998681]|677 HSP 1 e-value: 2.0E-19 bit: 97.6 Len: 120 Query Start:107 Query End:226 Subject Strand: POSITIVE Subject Start: 455 Subject End: 574 ..........................................................................................................tttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacg........................... ..........................................................................................................tttgtcattccgaacaccgtgaggaatctcgcaggggaaagggaag-agattcttcgctgacgctctgaatgacaaattaatggttaatggtcaatggttaattgttatgccgggtacacg........................... HSP 2 e-value: 4.0E-14 bit: 79.8 Len: 56 Query Start:1 Query End:56 Subject Strand: POSITIVE Subject Start: 160 Subject End: 215 tgaaagcctgacataattcaattgttaatggtcaattgttaattgttatgcagggt..................................................................................................................................................................................................... tgaaagcctgacgtaattcaattgttaattgtcaatggttaattgttatgccgggt..................................................................................................................................................................................................... HSP 3 e-value: 9.0E-6 bit: 52.0 Len: 42 Query Start:185 Query End:226 Subject Strand: POSITIVE Subject Start: 179 Subject End: 220 ........................................................................................................................................................................................aattgttaattgtcaatggtcaattattatgcgtggtacacg........................... ........................................................................................................................................................................................aattgttaattgtcaatggttaattgttatgccgggtacacg........................... Subject: tfor_TF2921_TF2922|hypothetical protein:surface antigen BspA|POSITIVE:POSITIVE|[3118851,3119261]|411 HSP 1 e-value: 7.0E-19 bit: 95.6 Len: 84 Query Start:38 Query End:121 Subject Strand: NEGATIVE Subject Start: 180 Subject End: 263 .....................................gttaattgttatgcagggttatccgaacgcaaataacgcaaataacgcgaatgaaagcaaataaccccctttgtcattccgaac.................................................................................................................................... .....................................gttaattgttatgcgtggttatccgaacgcaaatttcgcaaatgacgcgaatcaacgcaaataaccgcgtttgtcattccgaac.................................................................................................................................... HSP 2 e-value: 3.0E-18 bit: 93.7 Len: 55 Query Start:199 Query End:253 Subject Strand: NEGATIVE Subject Start: 41 Subject End: 95 ......................................................................................................................................................................................................aatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca ......................................................................................................................................................................................................aatggttaattgttatgcgtggtacacgccgaatcatgtatttagaaaataacca HSP 3 e-value: 2.0E-16 bit: 87.7 Len: 94 Query Start:104 Query End:197 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 175 .......................................................................................................ccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgt........................................................ .......................................................................................................ccctttgtcattccgaacgtagtgaggaatcccgtggggaaaggaaaa--agattcttcgcttacgctctgaatgacaaattaatggttaattgt........................................................ HSP 4 e-value: 7.0E-16 bit: 85.7 Len: 94 Query Start:104 Query End:197 Subject Strand: NEGATIVE Subject Start: 255 Subject End: 348 .......................................................................................................ccctttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgt........................................................ .......................................................................................................ccctttgtcattccgaacgtagtgaggaatctcacggggaagagggaag-agattcttcgcttacgctctgaatgacaaattaatggttaattgt........................................................ Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 1.0E-17 bit: 91.7 Len: 90 Query Start:108 Query End:197 Subject Strand: NEGATIVE Subject Start: 539 Subject End: 627 ...........................................................................................................ttgtcattccgaacacagtgaggaatcccgcgaggaaaaggaaaggagattcttcgctttgctctgaatgacaaattaattgttaattgt........................................................ ...........................................................................................................ttgtcattccgaacaccgtgaggaatctcgcggggtaaagggaag-agattcatcgcttcgctctgaatgacaaattaatggtcaattgt........................................................ HSP 2 e-value: 1.0E-14 bit: 81.8 Len: 49 Query Start:153 Query End:201 Subject Strand: NEGATIVE Subject Start: 158 Subject End: 206 ........................................................................................................................................................gagattcttcgctttgctctgaatgacaaattaattgttaattgtcaat.................................................... ........................................................................................................................................................gagattcttcgcttcgctctgaatgacaaattaatggttaattgtcaat.................................................... HSP 3 e-value: 1.0E-11 bit: 71.9 Len: 48 Query Start:4 Query End:51 Subject Strand: NEGATIVE Subject Start: 730 Subject End: 777 ...aagcctgacataattcaattgttaatggtcaattgttaattgttatgc.......................................................................................................................................................................................................... ...aagcctgacgtaattcaattgttaatggttaattgtcaattgttatgc.......................................................................................................................................................................................................... HSP 4 e-value: 2.0E-6 bit: 54.0 Len: 27 Query Start:25 Query End:51 Subject Strand: NEGATIVE Subject Start: 527 Subject End: 553 ........................ttaatggtcaattgttaattgttatgc.......................................................................................................................................................................................................... ........................ttaatggtcaattgttaattgttatgc.......................................................................................................................................................................................................... HSP 5 e-value: 9.0E-6 bit: 52.0 Len: 42 Query Start:185 Query End:226 Subject Strand: NEGATIVE Subject Start: 720 Subject End: 761 ........................................................................................................................................................................................aattgttaattgtcaatggtcaattattatgcgtggtacacg........................... ........................................................................................................................................................................................aattgttaatggttaattgtcaattgttatgcgtggtacacg........................... Subject: tfor_TF2500_TF2501|ABC transporter, ATP-binding protein:DNA mismatch repair protein|POSITIVE:POSITIVE|[2675363,2676419]|1057 HSP 1 e-value: 3.0E-15 bit: 83.8 Len: 77 Query Start:150 Query End:226 Subject Strand: POSITIVE Subject Start: 717 Subject End: 794 .....................................................................................................................................................aaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacg........................... .....................................................................................................................................................aaggagattcttcgcttacgctctgaatgacatattaatggttaattgtcaattgtcaattgttatgccgggtacacg........................... HSP 2 e-value: 2.0E-7 bit: 58.0 Len: 41 Query Start:1 Query End:41 Subject Strand: POSITIVE Subject Start: 294 Subject End: 334 tgaaagcctgacataattcaattgttaatggtcaattgtta.................................................................................................................................................................................................................... tgaaagcctgacgtaattcagttgttaattgtcaattgtta.................................................................................................................................................................................................................... HSP 3 e-value: 2.0E-6 bit: 54.0 Len: 47 Query Start:34 Query End:80 Subject Strand: POSITIVE Subject Start: 320 Subject End: 366 .................................aattgttaattgttatgcagggttatccgaacgcaaataacgcaaat............................................................................................................................................................................. .................................aattgtcaattgttatgcgtggttatccgaacgcaaatttcgcaaat............................................................................................................................................................................. Subject: tfor_TF1187_TF1188|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1269933,1270295]|363 HSP 1 e-value: 3.0E-15 bit: 83.8 Len: 107 Query Start:147 Query End:253 Subject Strand: POSITIVE Subject Start: 72 Subject End: 172 ..................................................................................................................................................ggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca ..................................................................................................................................................ggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaat-------ggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataacca Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 2.0E-7 bit: 58.0 Len: 33 Query Start:206 Query End:238 Subject Strand: NEGATIVE Subject Start: 413 Subject End: 445 .............................................................................................................................................................................................................aattattatgcgtggtacacgccgaatcatgta............... .............................................................................................................................................................................................................aattgttatgcgtggtacacgccgaatcatgta............... HSP 2 e-value: 2.0E-6 bit: 54.0 Len: 31 Query Start:11 Query End:41 Subject Strand: POSITIVE Subject Start: 225 Subject End: 255 ..........acataattcaattgttaatggtcaattgtta.................................................................................................................................................................................................................... ..........acataattcaattgttaattgtcaattgtta.................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAGCCUGACAUAAUUCAAUUGUUAAUGGUCAAUUGUUAAUUGUUAUGCAGGGUUAUCCGAACGCAAAUAACGCAAAUAACGCGAAUGAAAGCAAAUAACCCCCUUUGUCAUUCCGAACACAGUGAGGAAUCCCGCGAGGAAAAGGAAAGGAGAUUCUUCGCUUUGCUCUGAAUGACAAAUUAAUUGUUAAUUGUCAAUGGUCAAUUAUUAUGCGUGGUACACGCCGAAUCAUGUAUUUAGAAAAUAACCA .....((.(((((.....((((((........)))))).....))))).))..(((((((..((..((....((((((((((...((......((((.((((....((((((((((.((.((.(((((((((((((...............)).))))))))))).)).)).))))))))))......)))).))))......))..))))).)))))(((....)))......))..))..))...))))). (-66.86) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUUAUUUUCUAAAUACAUGAUUCGGCGUGUACCACGCAUAAUAAUUGACCAUUGACAAUUAACAAUUAAUUUGUCAUUCAGAGCAAAGCGAAGAAUCUCCUUUCCUUUUCCUCGCGGGAUUCCUCACUGUGUUCGGAAUGACAAAGGGGGUUAUUUGCUUUCAUUCGCGUUAUUUGCGUUAUUUGCGUUCGGAUAACCCUGCAUAACAAUUAACAAUUGACCAUUAACAAUUGAAUUAUGUCAGGCUUUCA ................(((((((((.(((((...)))))....((((((...((((...............((((((((((.(((((.((.((.((((((((.............).))))))).)).)).))))).)))))))))).(((((((((((..(.((..((((.....)))).....)).)..)))))))))))......))))...)))))).............))))))))).......... (-67.02) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 809 180 131 100 51 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 788 180 131 90 41 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 756 200 151 229 180 tfor:TF2669|5end_possible_Fe-S_oxidoreductase_2842971..2843201_POSITIVE 756 200 151 192 143 tfor:TF2668|5end_hypothetical_protein_2842471..2842701_POSITIVE 756 200 151 116 67 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 756 200 151 192 143 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 719 240 191 77 28 tfor:TF2670|5end_hypothetical_protein_2842779..2843009_POSITIVE 719 240 191 77 28 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 712 240 191 77 28 tfor:TF2664|5end_hypothetical_protein_2841399..2841629_POSITIVE 704 200 151 148 99 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 703 180 131 166 119 tfor:TF0017|5end_hypothetical_protein_10504..10734_POSITIVE 665 150 101 227 175 tfor:TF0018|5end_hypothetical_protein_11250..11480_POSITIVE 650 200 151 130 81 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 645 159 111 174 126 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 639 197 151 148 102 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 639 197 151 117 71 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 634 150 102 220 170 tfor:TF1593|5end_hypothetical_protein_1708859..1709089_POSITIVE 625 220 171 224 175 tfor:TF2997|5end_hypothetical_protein_3211408..3211638_POSITIVE 614 148 102 199 152 tfor:TF2922|5end_surface_antigen_BspA_3119122..3119352_POSITIVE 597 120 72 227 180 tfor:TF2996|5end_hypothetical_protein_3210802..3211032_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 756 200 151 97 48 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 746 170 121 147 98 tfor:TF0017|3end_hypothetical_protein_11231..11381_POSITIVE 735 144 101 150 110 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 735 144 101 150 110 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 727 144 105 150 111 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 704 200 151 97 48 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 700 249 203 72 26 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 688 240 191 131 82 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 682 144 101 150 110 tfor:TF2173|3end_hypothetical_protein_2346063..2346213_POSITIVE 682 144 101 150 110 tfor:TF0346|3end_hypothetical_protein_367316..367466_POSITIVE 663 160 111 143 96 tfor:TF0016|3end_hypothetical_protein_10581..10731_POSITIVE 649 90 45 132 87 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 641 150 103 144 95 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 640 250 206 47 3 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 640 250 206 106 62 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 614 150 102 76 27 tfor:TF1582|3end_hypothetical_protein_1690506..1690656_POSITIVE 584 68 21 123 70 tfor:TF2996|3end_hypothetical_protein_3211121..3211271_POSITIVE 578 238 194 94 49 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 575 250 201 50 1 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 572 150 101 143 94 tfor:TF1581|3end_hypothetical_protein_1689467..1689617_POSITIVE