Origin IGS: taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaccaaccgagtttttcactttcccataaccattgaccattaaaaattaacaatcagctcaaagcaaattataccgaaaaagcaactgcccgcaaaaggacagttgcttttttatacgtggtgttcggatgtccggatta Mask Tandem Repeat Region ================================================ taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Find is-nt database================================================ Query_seq: TF2792:TF2793|TF2792:TF2793:30S ribosomal protein S15:possible L-lysine 2,3-aminomutase:->->:2977660..2977800 141 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2792:TF2793|TF2792:TF2793:30S ribosomal protein S15:possible L-lysine 2,3-aminomutase:->->:2977660..2977800 141 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2792:TF2793|TF2792:TF2793:30S ribosomal protein S15:possible L-lysine 2,3-aminomutase:->->:2977660..2977800 141 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 3 End: 95 .. D P C G F V V Y L F A V T R K R A T A K E T Y N A K L Q N N M .............................................. taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 44 End: 73 ...........................................TTTTGCGGGCAGTTGCTTTTTCGGTATAAT.................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 62 HP_score: -7.4 Tail_Score: -3.08586 Start: 26 End: 67 Full_Region: ATCCGAACACCACGT ATAAAAAAGCAACTGTCC TTTTGC GGGCAGTTGCTTTTTCGG TATAATTTGCTTTGA .........................ATAAAAAAGCAACTGTCCTTTTGCGGGCAGTTGCTTTTTCGG.......................................................................... TransTerm Strand: - Conf: 100 HP_score: -9.0 Tail_Score: -5.28778 Start: 34 End: 59 Full_Region: aattataccgaaaaa gcaactgccc gcaaaa ggacagttgc ttttttatacgtggt .................................gcaactgcccgcaaaaggacagttgc.................................................................................. Find igs database================================================ Query_seq: TF2792:TF2793|TF2792:TF2793:30S ribosomal protein S15:possible L-lysine 2,3-aminomutase:->->:2977660..2977800 141 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Intra-Species Hit: Count: 1 Min: 1 Max: 141 Len: 141 Subject: tfor_TF2792_TF2793|30S ribosomal protein S15:possible L-lysine 2,3-aminomutase|POSITIVE:POSITIVE|[2977660,2977800]|141 HSP 1 e-value: 1.0E-74 bit: 280.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca taatccggacatccgaacaccacgtataaaaaagcaactgtccttttgcgggcagttgctttttcggtataatttgctttgagctgattgttaatttttaatggtcaatggttatgggaaagtgaaaaactcggttggtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCGGACAUCCGAACACCACGUAUAAAAAAGCAACUGUCCUUUUGCGGGCAGUUGCUUUUUCGGUAUAAUUUGCUUUGAGCUGAUUGUUAAUUUUUAAUGGUCAAUGGUUAUGGGAAAGUGAAAAACUCGGUUGGUCA .......(((..((((.......((((.(((((((((((((((......)))))))))))))))..))))..((..((((.(((((((..(((.....)))..)))...)))).....))))..))....))))...))). (-42.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACCAACCGAGUUUUUCACUUUCCCAUAACCAUUGACCAUUAAAAAUUAACAAUCAGCUCAAAGCAAAUUAUACCGAAAAAGCAACUGCCCGCAAAAGGACAGUUGCUUUUUUAUACGUGGUGUUCGGAUGUCCGGAUUA .(((...(((((....((((.....................................((.....))..........(((((((((((((.((......)).)))))))))))))....))))..)))))..)))....... (-32.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 344 68 31 87 54 tfor:TF0891|5end_hypothetical_protein_940509..940739_POSITIVE 339 69 30 214 175 tfor:TF2208|5end_conserved_hypothetical_protein_2382349..2382579_POSITIVE 331 66 21 219 177 tfor:TF2246|5end_hypothetical_protein_2421288..2421518_POSITIVE 329 86 41 193 139 tfor:TF1599|5end_ribonuclease_P_protein_component_1713564..1713794_POSITIVE 315 87 43 163 128 tfor:TF2473|5end_poly(A)_polymerase/tRNA_nucleotidyltransferase_2650470..2650700_POSITIVE 313 78 32 229 180 tfor:TF2936|5end_conserved_hypothetical_protein;_possible_ribosomal_subunit_interface_protein_3138522..3138752_POSITIVE 312 85 41 221 168 tfor:TF1984|5end_conserved_hypothetical_protein_2135278..2135508_POSITIVE 312 77 31 211 162 tfor:TF0543|5end_glycine_cleavage_system_p-protein_572251..572481_POSITIVE 311 81 48 159 131 tfor:TF0023|5end_possible_response_element_in_two_component_regulation_19446..19676_POSITIVE 309 67 22 170 114 tfor:TF0844|5end_transposase,_IS3_family_895204..895434_POSITIVE 307 88 42 88 44 tfor:TF1204|5end_possible_metal-dependent_membrane_protease_1286359..1286589_POSITIVE 304 68 28 159 121 tfor:TF0974|5end_hypothetical_protein_1024189..1024419_POSITIVE 299 90 41 203 154 tfor:TF3139|5end_Na+-translocating_NADH-quinone_reductase,_subunit_C_3372581..3372811_POSITIVE 299 80 34 98 31 tfor:TF0816|5end_hypothetical_protein_874217..874447_POSITIVE 297 51 11 131 94 tfor:TF2143|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_2319331..2319561_POSITIVE 295 118 73 123 62 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 295 89 41 48 6 tfor:TF0516|5end_conserved_hypothetical_protein;_possible_membrane_protein_539684..539914_POSITIVE 294 84 41 151 98 tfor:TF0380|5end_conserved_hypothetical_protein_400762..400992_POSITIVE 293 90 41 69 9 tfor:TF1830|5end_3-isopropylmalate_dehydratase,_large_subunit_1978888..1979118_POSITIVE 292 90 42 181 140 tfor:TF3141|5end_NADH:_ubiquinone_oxidoreductase,_subunit_A_3374667..3374897_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 412 66 23 68 25 tfor:TF2792|3end_30S_ribosomal_protein_S15_2977599..2977749_POSITIVE 344 68 31 53 20 tfor:TF0890|3end_transfer_region-related_protein,_TraQ_940555..940705_POSITIVE 331 66 21 142 100 tfor:TF2245|3end_conserved_hypothetical_protein_2421291..2421441_POSITIVE 322 75 31 77 24 tfor:TF2646|3end_conserved_hypothetical_protein_2816697..2816847_POSITIVE 315 87 43 81 46 tfor:TF2472|3end_GAF_domain-containing_protein_2650468..2650618_POSITIVE 309 67 22 113 57 tfor:TF0843|3end_transposase_895227..895377_POSITIVE 304 135 91 125 87 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 304 135 91 56 18 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 295 118 73 106 45 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 290 79 33 61 14 tfor:TF1818|3end_conserved_hypothetical_protein_1958754..1958904_POSITIVE 289 89 43 139 79 tfor:TF1187|3end_hypothetical_protein_1269872..1270022_POSITIVE 289 89 43 82 22 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 287 86 42 61 1 tfor:TF0061|3end_hypothetical_protein_65006..65156_POSITIVE 286 88 42 53 9 tfor:TF3110|3end_conserved_hypothetical_protein_with_helicase_domain_3346636..3346786_POSITIVE 286 85 43 140 103 tfor:TF1728|3end_cell_division_ABC_transporter,_ATP-binding_protein_FtsE_1853345..1853495_POSITIVE 284 54 11 136 93 tfor:TF1104|3end_hypothetical_protein_1172194..1172344_POSITIVE 283 77 38 57 16 tfor:TF2945|3end_DNA-directed_RNA_polymerase_beta'_subunit_3151802..3151952_POSITIVE 281 118 73 117 62 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 277 77 33 146 102 tfor:TF1292|3end_sensor_histidine_protein_kinase_1374554..1374704_POSITIVE 276 59 31 44 9 tfor:TF0180|3end_carboxynorspermidine_decarboxylase_192480..192630_POSITIVE