Origin IGS: taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttagtgcaaaagaacgatttatttgcggaacaacacaatatgttagtattatttaacgcgaacatcctaaacttttcgcagttttcgacatctcatacataatggcctgccccatctttatcgtgggcgattggccgaagaactcctgatgcttccgcctactcttccctaccgaccctctgtatggcattaaaaaaagtcgcctcctcgtcttaaggggcgactttttcctgtcttatctaagtacaacgactta Mask Tandem Repeat Region ================================================ taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Find is-nt database================================================ Query_seq: TF2768:TF2769|TF2768:TF2769:ferredoxin oxidoreductase, beta subunit:cell-division protein; ftsX family permease:->->:2955230..2955487 258 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2768:TF2769|TF2768:TF2769:ferredoxin oxidoreductase, beta subunit:cell-division protein; ftsX family permease:->->:2955230..2955487 258 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2768:TF2769|TF2768:TF2769:ferredoxin oxidoreductase, beta subunit:cell-division protein; ftsX family permease:->->:2955230..2955487 258 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 67 End: 201 .................................................................. M P Y R G S V G K S R R K H Q E F F G Q S P T I K M G Q A I M Y E M S K T A K S L G C S R ......................................................... Protein_Len: 32 Strand: - Start: 158 End: 253 ............................................................................................................................................................. T H S T S F Q S F N L I N A N F L V L M N H Q E A F L D N K Q M ..... Protein_Len: 32 Strand: - Start: 67 End: 162 .................................................................. H W V S P R Y P L T P P L M L L E E A L R G R Y L H P L G N H M ................................................................................................ taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 82 HP_score: -6.9 Tail_Score: -4.88711 Start: 33 End: 58 Full_Region: atggcattaaaaaaa gtcgcctcct cgtctta aggggcgac tttttcctgtcttat ................................gtcgcctcctcgtcttaaggggcgac........................................................................................................................................................................................................ TransTerm Strand: - Conf: 40 HP_score: -2.2 Tail_Score: -2.75992 Start: 98 End: 124 Full_Region: ttatcgtgggcgatt ggccgaagaa ctcctga tgcttccgcc tactcttccctaccg .................................................................................................ggccgaagaactcctgatgcttccgcc...................................................................................................................................... Find igs database================================================ Query_seq: TF2768:TF2769|TF2768:TF2769:ferredoxin oxidoreductase, beta subunit:cell-division protein; ftsX family permease:->->:2955230..2955487 258 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 258 Len: 258 Subject: tfor_TF2768_TF2769|ferredoxin oxidoreductase, beta subunit:cell-division protein; ftsX family permease|POSITIVE:POSITIVE|[2955230,2955487]|258 HSP 1 e-value: 1.0E-131 bit: 470.0 Len: 258 Query Start:1 Query End:258 Subject Strand: POSITIVE Subject Start: 1 Subject End: 258 taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgacnnnnnnnaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa taagtcgttgtacttagataagacaggaaaaagtcgccccttaagacgaggaggcgactttttttaatgccatacagagggtcggtagggaagagtaggcggaagcatcaggagttcttcggccaatcgcccacgataaagatggggcaggccattatgtatgagatgtcgaaaactgcgaaaagtttaggatgttcgcgttaaataatactaacatattgtgttgttccgcaaataaatcgttcttttgcactaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUCGUUGUACUUAGAUAAGACAGGAAAAAGUCGCCCCUUAAGACGAGGAGGCGACUUUUUUUAAUGCCAUACAGAGGGUCGGUAGGGAAGAGUAGGCGGAAGCAUCAGGAGUUCUUCGGCCAAUCGCCCACGAUAAAGAUGGGGCAGGCCAUUAUGUAUGAGAUGUCGAAAACUGCGAAAAGUUUAGGAUGUUCGCGUUAAAUAAUACUAACAUAUUGUGUUGUUCCGCAAAUAAAUCGUUCUUUUGCACUAAAA (((((......))))).....(((((((((((((((((((((.....)))).))))))))))))).....((((((...((((..............(((.((((.((.....)).)))).)))....((((.(......)...)))).))))....))))))...))))......(((.(((((...(.((((((.(((...((((((((.........)))))))).))).)))..))).).))))))))...... (-73.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAGUGCAAAAGAACGAUUUAUUUGCGGAACAACACAAUAUGUUAGUAUUAUUUAACGCGAACAUCCUAAACUUUUCGCAGUUUUCGACAUCUCAUACAUAAUGGCCUGCCCCAUCUUUAUCGUGGGCGAUUGGCCGAAGAACUCCUGAUGCUUCCGCCUACUCUUCCCUACCGACCCUCUGUAUGGCAUUAAAAAAAGUCGCCUCCUCGUCUUAAGGGGCGACUUUUUCCUGUCUUAUCUAAGUACAACGACUUA .....((((...(((.........((((((((.........(((((.(..((....))..).)))))........)))))))).....((((..(((((((..((((......)))).......(((((((...(((...((.....))...)))..)))))))..................)))))))......(((((((((((.(((.......))))))))))))))..))))...)))..))))......... (-60.43) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 484 148 102 81 28 tfor:TF1938|5end_hypothetical_protein_2085717..2085947_POSITIVE 459 150 105 186 122 tfor:TF1916|5end_conserved_hypothetical_protein;_possible_ATPase_2068530..2068760_POSITIVE 428 133 93 134 78 tfor:TF0719|5end_hypothetical_protein_769798..770028_POSITIVE 407 150 105 86 23 tfor:TF1925|5end_hypothetical_protein_2074458..2074688_POSITIVE 390 129 82 184 122 tfor:TF1932|5end_pigmentation_and_extracellular_proteinase_regulator_2078542..2078772_POSITIVE 377 164 121 54 14 tfor:TF2734|5end_conserved_hypothetical_protein_2916041..2916271_POSITIVE 374 138 100 52 7 tfor:TF1610|5end_peptide_chain_release_factor_RF-2_1728635..1728865_POSITIVE 372 109 62 171 120 tfor:TF2496|5end_butyryl-CoA_dehydrogenase,_short_chain_acyl-CoA_dehydrogenase_2670840..2671070_POSITIVE 371 150 101 126 78 tfor:TF2204|5end_conserved_hypothetical_protein_2378866..2379096_POSITIVE 367 130 81 94 32 tfor:TF1250|5end_possible_cation_efflux_pump_1332324..1332554_POSITIVE 364 130 81 195 154 tfor:TF0595|5end_hypothetical_protein_627820..628050_POSITIVE 362 136 92 162 127 tfor:TF1659|5end_hypothetical_protein_1774854..1775084_POSITIVE 358 106 68 121 84 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 357 120 71 89 36 tfor:TF1468|5end_beta-galactosidase_1557785..1558015_POSITIVE 355 129 81 194 142 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 353 129 81 180 128 tfor:TF2670|5end_hypothetical_protein_2842779..2843009_POSITIVE 353 137 98 67 38 tfor:TF1816|5end_hypothetical_protein_1957643..1957873_POSITIVE 353 137 98 48 19 tfor:TF1817|5end_hypothetical_protein_1957624..1957854_POSITIVE 352 160 112 160 114 tfor:TF3049|5end_conserved_hypothetical_protein_3276517..3276747_POSITIVE 352 136 92 47 2 tfor:TF1776|5end_urocanate_hydratase_1914660..1914890_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 459 150 105 65 1 tfor:TF1915|3end_NAD-dependent_protein_deacetylase,_SIR2_family_2068489..2068639_POSITIVE 419 130 83 146 80 tfor:TF1887|3end_hypothetical_protein_2040800..2040950_POSITIVE 398 129 83 86 41 tfor:TF1167|3end_hypothetical_protein_1248932..1249082_POSITIVE 372 109 62 142 91 tfor:TF2495|3end_succinate-semialdehyde_dehydrogenase_(NADP+)_2670891..2671041_POSITIVE 358 117 79 137 86 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 358 129 81 114 67 tfor:TF0899|3end_hypothetical_protein_946159..946309_POSITIVE 357 120 71 61 8 tfor:TF1467|3end_conserved_hypothetical_protein_1557837..1557987_POSITIVE 353 129 81 54 2 tfor:TF2668|3end_hypothetical_protein_2842733..2842883_POSITIVE 349 130 81 144 101 tfor:TF0467|3end_acetyltransferase-related_protein_486543..486693_POSITIVE 349 129 81 117 59 tfor:TF0320|3end_dihydroorotase_347518..347668_POSITIVE 348 144 105 150 107 tfor:TF2435|3end_DNA_repair_protein_radC_2615136..2615286_POSITIVE 348 116 72 144 100 tfor:TF1932|3end_pigmentation_and_extracellular_proteinase_regulator_2079755..2079905_POSITIVE 348 62 25 62 19 tfor:TF1255|3end_conserved_hypothetical_protein_1338151..1338301_POSITIVE 347 130 82 91 49 tfor:TF2909|3end_GTP-binding_protein,_possible_GTP1/OBG_family_3105956..3106106_POSITIVE 347 129 88 151 101 tfor:TF0371|3end_beta-mannosidase_precursor_390469..390619_POSITIVE 346 63 26 63 20 tfor:TF2768|3end_ferredoxin_oxidoreductase,_beta_subunit_2955169..2955319_POSITIVE 346 148 111 51 6 tfor:TF1314|3end_conserved_hypothetical_protein_1390302..1390452_POSITIVE 345 129 81 54 2 tfor:TF2666|3end_hypothetical_protein_2842263..2842413_POSITIVE 344 129 82 148 110 tfor:TF2738|3end_30S_ribosomal_protein_S16_2920084..2920234_POSITIVE 344 95 53 43 4 tfor:TF1459|3end_possible_protease_1549320..1549470_POSITIVE