Origin IGS: attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttttactgatttaaaattaataatgtttcgaaagtataaataaaaaacgaaccctctataaagcccttaggattttgtgttttttatatttatttagcaatctcgaagcaaatatctaatcaatgaataattttccgttggaaatttgttttatcggaaatatagttttatctttgcgccattatttccattggaacataataaaacaacatataggagaaaatttattctagaaagaat Mask Tandem Repeat Region ================================================ attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Find is-nt database================================================ Query_seq: TF2763:TF2764|TF2763:TF2764:pyridine nucleotide-disulphide oxidoreductase family protein:hypothetical protein:->->:2948655..2948896 242 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2763:TF2764|TF2763:TF2764:pyridine nucleotide-disulphide oxidoreductase family protein:hypothetical protein:->->:2948655..2948896 242 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2763:TF2764|TF2763:TF2764:pyridine nucleotide-disulphide oxidoreductase family protein:hypothetical protein:->->:2948655..2948896 242 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 11 End: 118 .......... F L N E G I H Q K I I N W H F Y H R L S L V I N G I F C I E L P F N N M ............................................................................................................................ Protein_Len: 31 Strand: - Start: 3 End: 95 .. R E L I F K R R Y T T K N H E L P F L P A F I F S Y K R Y F M ................................................................................................................................................... attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 79 HP_score: -6.3 Tail_Score: -4.77173 Start: 97 End: 109 Full_Region: taatcaatgaataat tttcc gtt ggaaa tttgttttatcggaa ................................................................................................tttccgttggaaa..................................................................................................................................... Find igs database================================================ Query_seq: TF2763:TF2764|TF2763:TF2764:pyridine nucleotide-disulphide oxidoreductase family protein:hypothetical protein:->->:2948655..2948896 242 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 242 Len: 242 Subject: tfor_TF2763_TF2764|pyridine nucleotide-disulphide oxidoreductase family protein:hypothetical protein|POSITIVE:POSITIVE|[2948655,2948896]|242 HSP 1 e-value: 1.0E-134 bit: 480.0 Len: 242 Query Start:1 Query End:242 Subject Strand: POSITIVE Subject Start: 1 Subject End: 242 attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa attctttctagaataaattttctcctatatgttgttttattatgttccaatggaaataatggcgcaaagataaaactatatttccgataaaacaaatttccaacggaaaattattcattgattagatatttgcttcgagattgctaaataaatataaaaaacacaaaatcctaagggctttatagagggttcgttttttatttatactttcgaaacattattaattttaaatcagtaaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCUUUCUAGAAUAAAUUUUCUCCUAUAUGUUGUUUUAUUAUGUUCCAAUGGAAAUAAUGGCGCAAAGAUAAAACUAUAUUUCCGAUAAAACAAAUUUCCAACGGAAAAUUAUUCAUUGAUUAGAUAUUUGCUUCGAGAUUGCUAAAUAAAUAUAAAAAACACAAAAUCCUAAGGGCUUUAUAGAGGGUUCGUUUUUUAUUUAUACUUUCGAAACAUUAUUAAUUUUAAAUCAGUAAAAAA (((((....))))).((((((((........((((((((((..........(((((((.(((.............)))))))))))))))))))).........))))))))....(((((((.((.(((((.(((((((.((.((((((((......((((....((((((...(......)..)))))).)))))))))))))).))))))).)).....))).)).)))))))...... (-35.35) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUUACUGAUUUAAAAUUAAUAAUGUUUCGAAAGUAUAAAUAAAAAACGAACCCUCUAUAAAGCCCUUAGGAUUUUGUGUUUUUUAUAUUUAUUUAGCAAUCUCGAAGCAAAUAUCUAAUCAAUGAAUAAUUUUCCGUUGGAAAUUUGUUUUAUCGGAAAUAUAGUUUUAUCUUUGCGCCAUUAUUUCCAUUGGAACAUAAUAAAACAACAUAUAGGAGAAAAUUUAUUCUAGAAAGAAU ((((((..(((((....(((.....(((((((((((((...((((((((((.....((((.........))))......))))))))))...))))).......)))))))))))....)))))..((((((((((((((((......(((((((..(((((((..((.........)).....)))))))..)))))))........)))).....))))))...))))))..)))))).. (-39.45) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 585 210 161 216 165 tfor:TF2541|5end_transposase_2723914..2724144_POSITIVE 337 219 175 61 12 tfor:TF2358|5end_possible_small-conductance_mechanosensitive_channel_2523829..2524059_POSITIVE 280 211 173 86 50 tfor:TF1448|5end_possible_competence_protein_1531711..1531941_POSITIVE 259 87 42 149 104 tfor:TF2669|5end_possible_Fe-S_oxidoreductase_2842971..2843201_POSITIVE 258 145 101 99 37 tfor:TF2742|5end_single-strand_DNA-specific_exonuclease_2923802..2924032_POSITIVE 258 198 162 52 10 tfor:TF0331|5end_hypothetical_protein_359074..359304_POSITIVE 240 196 167 72 42 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 239 106 62 165 124 tfor:TF2112|5end_glycine_cleavage_H_protein_2282119..2282349_POSITIVE 236 106 61 219 170 tfor:TF2920|5end_conserved_hypothetical_protein_3115903..3116133_POSITIVE 236 87 44 208 164 tfor:TF1529|5end_hypothetical_protein_1634300..1634530_POSITIVE 236 69 21 145 99 tfor:TF1067|5end_conserved_hypothetical_protein_1136867..1137097_POSITIVE 231 69 23 56 14 tfor:TF0287|5end_peptidase,_M23_family_315810..316040_POSITIVE 225 101 61 139 104 tfor:TF0403|5end_TPR_repeat_protein_422600..422830_POSITIVE 221 66 23 88 51 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 220 203 169 87 53 tfor:TF1342|5end_possible_lipoprotein_1423125..1423355_POSITIVE 219 200 163 75 34 tfor:TF2421|5end_cytocidal_toxin_protein_2599550..2599780_POSITIVE 219 193 168 226 200 tfor:TF1047|5end_tRNA_pseudouridylate_synthetase_A_(pseudouridylate_synthase_I)_1108483..1108713_POSITIVE 217 218 175 71 19 tfor:TF2438|5end_hypothetical_protein_2615781..2616011_POSITIVE 215 65 26 110 61 tfor:TF0975|5end_hypothetical_protein_1023994..1024224_POSITIVE 212 209 168 41 3 tfor:TF0417|5end_hypothetical_protein_433811..434041_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 585 210 161 58 7 tfor:TF2540|3end_conserved_hypothetical_protein_2723836..2723986_POSITIVE 344 87 45 102 46 tfor:TF2659|3end_conserved_hypothetical_protein_2833055..2833205_POSITIVE 337 219 175 52 3 tfor:TF2357|3end_collagenase_2523900..2524050_POSITIVE 292 198 167 62 30 tfor:TF1838|3end_tRNA/rRNA_methyltransferase_1988745..1988895_POSITIVE 280 211 173 59 23 tfor:TF1447|3end_D-ribulose-phosphate-3_epimerase_1531764..1531914_POSITIVE 259 87 42 136 91 tfor:TF2670|3end_hypothetical_protein_2843038..2843188_POSITIVE 258 145 101 95 33 tfor:TF2741|3end_ATP-dependent_DNA_helicase_RecQ_2923878..2924028_POSITIVE 258 198 162 44 2 tfor:TF0330|3end_excinuclease_ABC,_subunit_A_359146..359296_POSITIVE 240 89 45 57 7 tfor:TF0586|3end_conserved_hypothetical_protein_618409..618559_POSITIVE 239 106 62 81 40 tfor:TF2111|3end_RNA_polymerase_sigma-54_factor_2282115..2282265_POSITIVE 236 69 21 56 10 tfor:TF1066|3end_hypothetical_protein_1136858..1137008_POSITIVE 225 101 61 68 33 tfor:TF0402|3end_conserved_hypothetical_protein_422609..422759_POSITIVE 224 99 61 40 2 tfor:TF0602|3end_aminotripeptidase;_peptidase_T_633472..633622_POSITIVE 220 203 169 82 48 tfor:TF1341|3end_glycogen_synthase_1423200..1423350_POSITIVE 219 212 178 117 77 tfor:TF3010|3end_possible_anti-sigma_factor_3230888..3231038_POSITIVE 219 193 168 109 83 tfor:TF1046|3end_hypothetical_protein_1108446..1108596_POSITIVE 215 65 26 137 88 tfor:TF0973|3end_conserved_hypothetical_protein_1024101..1024251_POSITIVE 213 140 93 93 35 tfor:TF0100|3end_conserved_hypothetical_protein_111703..111853_POSITIVE 209 143 101 65 7 tfor:TF2931|3end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3135584..3135734_POSITIVE 209 101 63 44 3 tfor:TF2121|3end_phosphoenolpyruvate_synthase_2297225..2297375_POSITIVE