Origin IGS: taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatccggaagaagtgaaccgtccgacagatcaggaagaagagaggggggcgaaaccggacagggaagccctccttttttgatggagcaggctgtcgaatgaatcgacatta Mask Tandem Repeat Region ================================================ taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Find is-nt database================================================ Query_seq: TF2746:TF2747|TF2746:TF2747:hypothetical protein:heat shock protein, HSP90 family:->->:2928307..2928418 112 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2746:TF2747|TF2746:TF2747:hypothetical protein:heat shock protein, HSP90 family:->->:2928307..2928418 112 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2746:TF2747|TF2746:TF2747:hypothetical protein:heat shock protein, HSP90 family:->->:2928307..2928418 112 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 3 End: 110 .. M S I H S T A C S I K K G G L P C P V S P P S L L P D L S D G S L L P D .. Protein_Len: 33 Strand: + Start: 13 End: 111 ............ M R Q P A P S K K E G F P V R F R P P L F F L I C R T V H F F R I . taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2746:TF2747|TF2746:TF2747:hypothetical protein:heat shock protein, HSP90 family:->->:2928307..2928418 112 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: tfor_TF2746_TF2747|hypothetical protein:heat shock protein, HSP90 family|POSITIVE:POSITIVE|[2928307,2928418]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta taatgtcgattcattcgacagcctgctccatcaaaaaaggagggcttccctgtccggtttcgcccccctctcttcttcctgatctgtcggacggttcacttcttccggatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGUCGAUUCAUUCGACAGCCUGCUCCAUCAAAAAAGGAGGGCUUCCCUGUCCGGUUUCGCCCCCCUCUCUUCUUCCUGAUCUGUCGGACGGUUCACUUCUUCCGGAUUA ...((((((.....)))))).................(((.((((..((......))....)))).)))..........(((((((..(((.(.....).)))..))))))) (-27.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCGGAAGAAGUGAACCGUCCGACAGAUCAGGAAGAAGAGAGGGGGGCGAAACCGGACAGGGAAGCCCUCCUUUUUUGAUGGAGCAGGCUGUCGAAUGAAUCGACAUUA .....(....)..((((..(((.((((((.((......(((((((((((((....((.....))...)))))))))))))....)).....)))))).)))..)).)).... (-36.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 411 77 37 168 121 tfor:TF0811|5end_conserved_hypothetical_protein;_posible_sugar_phosphate_isomerase_869787..870017_POSITIVE 394 109 61 57 14 tfor:TF2511|5end_RNA_binding_protein_2689910..2690140_POSITIVE 391 90 41 204 136 tfor:TF1928|5end_conserved_hypothetical_protein_2075529..2075759_POSITIVE 390 100 54 156 97 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 389 60 14 183 129 tfor:TF1329|5end_glucose-1-phosphate_thymidyltransferase_1408578..1408808_POSITIVE 388 95 54 82 38 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 381 45 5 161 125 tfor:TF2739|5end_thioredoxin_2920126..2920356_POSITIVE 379 96 54 220 183 tfor:TF1833|5end_2-isopropylmalate_synthase_1981511..1981741_POSITIVE 377 70 21 227 167 tfor:TF0830|5end_hypothetical_protein_887610..887840_POSITIVE 376 69 22 164 107 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 366 89 41 68 1 tfor:TF0336|5end_Xaa-Pro_aminopeptidase_360613..360843_POSITIVE 365 100 51 159 95 tfor:TF0366|5end_hypothetical_protein_383564..383794_POSITIVE 365 100 51 159 95 tfor:TF0362|5end_hypothetical_protein_379952..380182_POSITIVE 361 110 61 74 17 tfor:TF0414|5end_N-ethylammeline_chlorohydrolase_431789..432019_POSITIVE 356 64 24 214 171 tfor:TF3065|5end_enolase_3291055..3291285_POSITIVE 353 108 64 80 8 tfor:TF0133|5end_transposase_element_145174..145404_POSITIVE 350 90 42 194 130 tfor:TF3072|5end_conserved_hypothetical_protein_3295302..3295532_POSITIVE 349 90 43 203 142 tfor:TF0543|5end_glycine_cleavage_system_p-protein_572251..572481_POSITIVE 348 55 11 70 22 tfor:TF1386|5end_hypothetical_protein_1460103..1460333_POSITIVE 348 109 61 187 132 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 45 5 39 3 tfor:TF2738|3end_30S_ribosomal_protein_S16_2920084..2920234_POSITIVE 379 96 54 131 94 tfor:TF1832|3end_3-isopropylmalate_dehydratase,_small_subunit_1981502..1981652_POSITIVE 373 69 21 71 13 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 373 69 21 90 32 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 369 69 22 149 93 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 366 89 41 86 19 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 364 90 42 97 36 tfor:TF0975|3end_hypothetical_protein_1024361..1024511_POSITIVE 361 110 61 74 17 tfor:TF0413|3end_succinyl-diaminopimelate_desuccinylase_431869..432019_POSITIVE 360 90 41 84 26 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 358 50 3 66 12 tfor:TF1382|3end_hypothetical_protein_1457777..1457927_POSITIVE 358 50 3 62 8 tfor:TF1383|3end_hypothetical_protein_1457773..1457923_POSITIVE 358 74 36 49 6 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 356 64 24 63 20 tfor:TF3064|3end_oxidoreductase,_aldo/keto_reductase_family_3290984..3291134_POSITIVE 348 109 61 110 55 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 346 108 61 81 32 tfor:TF1326|3end_hypothetical_protein_1404100..1404250_POSITIVE 345 97 51 136 61 tfor:TF2042|3end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2202356..2202506_POSITIVE 344 60 18 88 29 tfor:TF3165|3end_thiol:disulfide_interchange_protein,_thioredoxin_family_protein_3405343..3405493_POSITIVE 344 69 24 135 89 tfor:TF1842|3end_hypothetical_protein_1992800..1992950_POSITIVE 343 90 41 102 52 tfor:TF2556|3end_conserved_hypothetical_protein_2737095..2737245_POSITIVE 341 109 62 60 13 tfor:TF2789|3end_enoyl-[acyl-carrier-protein]_reductase_2973365..2973515_POSITIVE