Origin IGS: atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaaccattaatttgtcattcagagcgaagcgatgaatctctctccttttccggggcgagattcctcacggtgttcggaatgacaaaggcggcgttcgtaatgcccataccttgttcagagggctgaaggcctgacataatcgtcagcgttcagcattcagttagtcagcgttcagcaaacaattcaacacctaaacccctcacccccctccattcttatttcttctattgcggaatctgcgattaaaaattatcgcgtacat Mask Tandem Repeat Region ================================================ atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Find is-nt database================================================ Query_seq: TF2744:TF2745|TF2744:TF2745:methionyl-tRNA formyltransferase:hypothetical protein:->->:2927439..2927701 263 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2744:TF2745|TF2744:TF2745:methionyl-tRNA formyltransferase:hypothetical protein:->->:2927439..2927701 263 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2744:TF2745|TF2744:TF2745:methionyl-tRNA formyltransferase:hypothetical protein:->->:2927439..2927701 263 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 117 End: 245 .................................................................................................................... M T I M S G L Q P S E Q G M G I T N A A F V I P N T V R N L A P E K E R D S S L R S E .................. Protein_Len: 38 Strand: - Start: 140 End: 253 ........................................................................................................................................... G E S C P I P M V F A A K T M G F V T L F R A G S F S L S E D S R E S H C M .......... Protein_Len: 32 Strand: - Start: 132 End: 227 ................................................................................................................................... A K L G R F L T H A N R V G G K D N R V G H P I E G R F L L S M .................................... Protein_Len: 36 Strand: - Start: 20 End: 127 ................... D C I G C Y F F Y S H L P T L P K P T S N N A S R Q S V S H Q V S V I M ........................................................................................................................................ Protein_Len: 36 Strand: - Start: 1 End: 108 H V R Y N K I A S E A I S S I L I S P P S P N L H Q I T Q Q V S V L Q M ........................................................................................................................................................... atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 48 HP_score: -4.3 Tail_Score: -3.16742 Start: 103 End: 147 Full_Region: tgcccataccttgtt cagagggctgaaggcctgac ataatc gtcagcgttcagcattcag ttagtcagcgttcag ......................................................................................................cagagggctgaaggcctgacataatcgtcagcgttcagcattcag.................................................................................................................... TransTerm Strand: - Conf: 46 HP_score: -3.6 Tail_Score: -3.20723 Start: 108 End: 142 Full_Region: ataccttgttcagag ggctgaaggcctgac ataatc gtcagcgttcagca ttcagttagtcagcg ...........................................................................................................ggctgaaggcctgacataatcgtcagcgttcagca......................................................................................................................... TransTerm Strand: + Conf: 60 HP_score: -7.1 Tail_Score: -3.1355 Start: 195 End: 234 Full_Region: GTCATTCCGAACACC GTGAGGAATCTCGCCCC GGAAAA GGAGAGAGATTCATCGC TTCGCTCTGAATGAC ..................................................................................................................................................................................................GTGAGGAATCTCGCCCCGGAAAAGGAGAGAGATTCATCGC............................. Find igs database================================================ Query_seq: TF2744:TF2745|TF2744:TF2745:methionyl-tRNA formyltransferase:hypothetical protein:->->:2927439..2927701 263 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Intra-Species Hit: Count: 3 Min: 1 Max: 263 Len: 263 Subject: tfor_TF2744_TF2745|methionyl-tRNA formyltransferase:hypothetical protein|POSITIVE:POSITIVE|[2927439,2927701]|263 HSP 1 e-value: 1.0E-147 bit: 521.0 Len: 263 Query Start:1 Query End:263 Subject Strand: POSITIVE Subject Start: 1 Subject End: 263 atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa atgtacgcgataatttttaatcgcagattccgcaatagaagaaataagaatggaggggggtgaggggtttaggtgttgaattgtttgctgaacgctgactaactgaatgctgaacgctgacgattatgtcaggccttcagccctctgaacaaggtatgggcattacgaacgccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 3.0E-24 bit: 113.0 Len: 93 Query Start:171 Query End:263 Subject Strand: POSITIVE Subject Start: 218 Subject End: 310 ..........................................................................................................................................................................gccgcctttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa ..........................................................................................................................................................................gccgtctttgtcattccgaacgtagtgaggaatcccgccccggtaaagggaagagattcttcgcttcgctctgaatgacaaattaatggttaa HSP 2 e-value: 2.0E-6 bit: 54.0 Len: 27 Query Start:26 Query End:52 Subject Strand: NEGATIVE Subject Start: 601 Subject End: 627 .........................gattccgcaatagaagaaataagaatg................................................................................................................................................................................................................... .........................gattccgcaatagaagaaataagaatg................................................................................................................................................................................................................... Subject: tfor_TF2823_TF2824|TPR-containing protein:nucleoside-transporting protein nupG|POSITIVE:POSITIVE|[3014398,3014840]|443 HSP 1 e-value: 1.0E-23 bit: 111.0 Len: 103 Query Start:161 Query End:263 Subject Strand: POSITIVE Subject Start: 87 Subject End: 189 ................................................................................................................................................................cattacgaacgccgcc-tttgtcattccgaacaccgtgaggaatctcgccccggaaaaggagagagattcatcgcttcgctctgaatgacaaattaatggttaa ................................................................................................................................................................cattacgaacgccaccctttgtcattccgaacgcagtgaggaatctcgcgg-ggaaaagggaagagattcttcgcttcgctctgaatgacaaattaatggttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGUACGCGAUAAUUUUUAAUCGCAGAUUCCGCAAUAGAAGAAAUAAGAAUGGAGGGGGGUGAGGGGUUUAGGUGUUGAAUUGUUUGCUGAACGCUGACUAACUGAAUGCUGAACGCUGACGAUUAUGUCAGGCCUUCAGCCCUCUGAACAAGGUAUGGGCAUUACGAACGCCGCCUUUGUCAUUCCGAACACCGUGAGGAAUCUCGCCCCGGAAAAGGAGAGAGAUUCAUCGCUUCGCUCUGAAUGACAAAUUAAUGGUUAA ......(((((........))))).....(((.....................((((((.((((((((((((.((((.(.(((((.((.....)).))).)).).)))))))))).(((((......))))).)))))).))))))....(((((...(((.........))))))))((((((((.((.(...((((.(((((((.(.((......)).).))))))).))))...).)).))))))))......))).... (-77.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAACCAUUAAUUUGUCAUUCAGAGCGAAGCGAUGAAUCUCUCUCCUUUUCCGGGGCGAGAUUCCUCACGGUGUUCGGAAUGACAAAGGCGGCGUUCGUAAUGCCCAUACCUUGUUCAGAGGGCUGAAGGCCUGACAUAAUCGUCAGCGUUCAGCAUUCAGUUAGUCAGCGUUCAGCAAACAAUUCAACACCUAAACCCCUCACCCCCCUCCAUUCUUAUUUCUUCUAUUGCGGAAUCUGCGAUUAAAAAUUAUCGCGUACAU ....((.....((((((((((.(((((..(.((.(((((((.((((......)))).))))))).)).)..))))).))))))))))((.(((((.....))))).............((((((((((.((.((((......)))))).)))))).........((..((.....))..)).................))))........))....................)).....(((((........)))))...... (-77.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 896 225 181 231 187 tfor:TF0018|5end_hypothetical_protein_11250..11480_POSITIVE 768 208 161 58 10 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 753 208 166 48 5 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 752 208 162 87 41 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 743 240 191 162 114 tfor:TF1590|5end_hypothetical_protein_1705811..1706041_POSITIVE 733 260 211 120 75 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 728 250 201 172 124 tfor:TF2668|5end_hypothetical_protein_2842471..2842701_POSITIVE 728 250 201 96 48 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 723 230 181 152 104 tfor:TF1592|5end_hypothetical_protein_1708325..1708555_POSITIVE 714 250 201 177 124 tfor:TF1585|5end_hypothetical_protein_1690369..1690599_POSITIVE 714 250 201 177 124 tfor:TF1584|5end_hypothetical_protein_1690245..1690475_POSITIVE 714 250 201 177 124 tfor:TF1582|5end_hypothetical_protein_1690121..1690351_POSITIVE 711 240 191 156 109 tfor:TF0017|5end_hypothetical_protein_10504..10734_POSITIVE 697 208 166 189 146 tfor:TF2922|5end_surface_antigen_BspA_3119122..3119352_POSITIVE 683 250 201 196 146 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 680 250 201 209 161 tfor:TF2669|5end_possible_Fe-S_oxidoreductase_2842971..2843201_POSITIVE 680 250 201 172 124 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 676 149 102 226 175 tfor:TF0341|5end_conserved_hypothetical_protein_364348..364578_POSITIVE 649 260 211 224 172 tfor:TF2923|5end_hypothetical_protein_3122168..3122398_POSITIVE 648 180 131 196 148 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 970 240 191 147 98 tfor:TF0017|3end_hypothetical_protein_11231..11381_POSITIVE 910 220 171 143 94 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 792 160 111 66 17 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 714 250 201 75 22 tfor:TF1583|3end_conserved_hypothetical_protein;_possible_endonuclease_1690223..1690373_POSITIVE 701 229 181 142 96 tfor:TF0016|3end_hypothetical_protein_10581..10731_POSITIVE 697 260 211 87 36 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 682 260 212 74 27 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 672 250 201 110 57 tfor:TF1582|3end_hypothetical_protein_1690506..1690656_POSITIVE 652 215 174 150 108 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 648 260 212 53 3 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 642 215 174 150 108 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 642 215 174 150 108 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 616 260 214 74 22 tfor:TF1576|3end_hypothetical_protein_1686135..1686285_POSITIVE 591 214 175 57 18 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 591 220 172 142 96 tfor:TF1581|3end_hypothetical_protein_1689467..1689617_POSITIVE 589 208 161 144 99 tfor:TF2173|3end_hypothetical_protein_2346063..2346213_POSITIVE 589 208 161 144 99 tfor:TF0346|3end_hypothetical_protein_367316..367466_POSITIVE 587 257 211 84 43 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 550 180 131 138 90 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 539 214 171 110 58 tfor:TF2653|3end_hypothetical_protein_2822460..2822610_POSITIVE