Origin IGS: cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gacgcaaagatagcattccctgctgaaagcagtatctctccaaccttgaaaaatgtgtgtatatgaaataaagatatttaatttttaagcgcttgaggtaggacgaagcataaaatcttttttctttgcacccggtattaatcttttaaaataacagaacg Mask Tandem Repeat Region ================================================ cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Find is-nt database================================================ Query_seq: TF2699:TF2700|TF2699:TF2700:hypothetical protein:possible nifS-like aminotransferase:->->:2872834..2872994 161 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2699:TF2700|TF2699:TF2700:hypothetical protein:possible nifS-like aminotransferase:->->:2872834..2872994 161 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2699:TF2700|TF2699:TF2700:hypothetical protein:possible nifS-like aminotransferase:->->:2872834..2872994 161 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 20 End: 160 ................... M N T G C K E K R F Y A S S Y L K R L K I K Y L Y F I Y T H F S R L E R Y C F Q Q G M L S L R . cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2699:TF2700|TF2699:TF2700:hypothetical protein:possible nifS-like aminotransferase:->->:2872834..2872994 161 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Intra-Species Hit: Count: 1 Min: 1 Max: 161 Len: 161 Subject: tfor_TF2699_TF2700|hypothetical protein:possible nifS-like aminotransferase|POSITIVE:POSITIVE|[2872834,2872994]|161 HSP 1 e-value: 2.0E-86 bit: 319.0 Len: 161 Query Start:1 Query End:161 Subject Strand: POSITIVE Subject Start: 1 Subject End: 161 cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc cgttctgttattttaaaagattaataccgggtgcaaagaaaaaagattttatgcttcgtcctacctcaagcgcttaaaaattaaatatctttatttcatatacacacatttttcaaggttggagagatactgctttcagcagggaatgctatctttgcgtc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUCUGUUAUUUUAAAAGAUUAAUACCGGGUGCAAAGAAAAAAGAUUUUAUGCUUCGUCCUACCUCAAGCGCUUAAAAAUUAAAUAUCUUUAUUUCAUAUACACACAUUUUUCAAGGUUGGAGAGAUACUGCUUUCAGCAGGGAAUGCUAUCUUUGCGUC ...((((.((((..........)))).)))).(((((((..(((((((((((((((.(........)))))).........)))).))))))..(((..........(((((((.......))))))).((((.....)))).))).....)))))))... (-24.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGCAAAGAUAGCAUUCCCUGCUGAAAGCAGUAUCUCUCCAACCUUGAAAAAUGUGUGUAUAUGAAAUAAAGAUAUUUAAUUUUUAAGCGCUUGAGGUAGGACGAAGCAUAAAAUCUUUUUUCUUUGCACCCGGUAUUAAUCUUUUAAAAUAACAGAACG ...(((((((.........((((.....))))....(((((.((((..(.......((((.((.(((.((((....)))).))).)).)))))..)))).))).))................)))))))................................ (-28.61) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 159 118 186 130 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 323 68 23 202 158 tfor:TF0444|5end_Na+-translocating_NADH-quinone_oxidoreductase,_subunit_5_465869..466099_POSITIVE 319 158 119 58 16 tfor:TF1602|5end_L-aspartate_oxidase_1715579..1715809_POSITIVE 318 160 115 91 42 tfor:TF2515|5end_outer_membrane_protein,_TonB_dependent_receptor_2692854..2693084_POSITIVE 300 158 120 42 2 tfor:TF1084|5end_transposase_1153310..1153540_POSITIVE 294 159 116 86 42 tfor:TF2224|5end_CTn_excision_protein_2394592..2394822_POSITIVE 287 160 112 180 127 tfor:TF2012|5end_conserved_hypothetical_protein_2165380..2165610_POSITIVE 272 158 118 196 145 tfor:TF0692|5end_conserved_hypothetical_protein_736379..736609_POSITIVE 271 159 116 185 142 tfor:TF2278|5end_conserved_hypothetical_protein_2438662..2438892_POSITIVE 271 153 117 146 107 tfor:TF0802|5end_glucose-1-phosphate_cytidylyltransferase_860310..860540_POSITIVE 270 160 126 171 139 tfor:TF2248|5end_transfer_region-related_protein,_TraB_2422551..2422781_POSITIVE 269 77 32 87 34 tfor:TF1270|5end_hypothetical_protein_1355273..1355503_POSITIVE 267 140 96 51 7 tfor:TF0424|5end_outer_membrane_receptor,_TonB-linked_440028..440258_POSITIVE 266 69 27 54 15 tfor:TF1974|5end_glyoxalase_I_related_protein_(lactoylglutathione_lyase)_2120950..2121180_POSITIVE 264 160 117 186 145 tfor:TF0142|5end_subtilisin-like_serine_proteinase_149740..149970_POSITIVE 263 159 116 57 9 tfor:TF1488|5end_conserved_hypothetical_protein_1588464..1588694_POSITIVE 260 76 34 66 24 tfor:TF1938|5end_hypothetical_protein_2085717..2085947_POSITIVE 258 159 111 208 156 tfor:TF2907|5end_CDP-diacylglycerol-serine-O-phosphatidyltransfera_se_3102861..3103091_POSITIVE 257 159 117 61 14 tfor:TF2284|5end_hypothetical_protein_2440557..2440787_POSITIVE 257 66 27 154 113 tfor:TF1814|5end_conserved_hypothetical_protein;_possible_biotin_synthase_related_domain_containing_protein_1955325..1955555_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 160 114 144 94 tfor:TF1730|3end_diaminopimelate_decarboxylase_1855907..1856057_POSITIVE 308 150 107 76 36 tfor:TF1412|3end_conserved_hypothetical_protein_1493256..1493406_POSITIVE 294 159 116 46 2 tfor:TF2223|3end_CTn_excision__protein_2394632..2394782_POSITIVE 281 156 114 151 100 tfor:TF0075|3end_transcriptional_regulator_83118..83268_POSITIVE 271 153 117 83 44 tfor:TF0801|3end_transport_permease_860327..860477_POSITIVE 269 160 117 52 5 tfor:TF1653|3end_hypothetical_protein_1766481..1766631_POSITIVE 269 160 117 52 5 tfor:TF0979|3end_hypothetical_protein_1033118..1033268_POSITIVE 269 160 118 151 107 tfor:TF0181|3end_ATP-dependent_DNA_helicase_194863..195013_POSITIVE 268 159 114 129 86 tfor:TF3141|3end_NADH:_ubiquinone_oxidoreductase,_subunit_A_3376267..3376417_POSITIVE 257 159 117 70 26 tfor:TF2690|3end_possible_permease_2866562..2866712_POSITIVE 257 66 27 42 1 tfor:TF1813|3end_peptide_methionine_sulfoxide_reductase_1955293..1955443_POSITIVE 256 159 116 46 5 tfor:TF2275|3end_conserved_hypothetical_protein_2438300..2438450_POSITIVE 255 159 117 95 49 tfor:TF2109|3end_possible_metallo-beta-lactamase_family_protein_2278960..2279110_POSITIVE 254 159 125 61 23 tfor:TF0311|3end_conserved_hypothetical_protein_336383..336533_POSITIVE 253 160 120 123 81 tfor:TF1303|3end_conserved_hypothetical_protein_1384698..1384848_POSITIVE 250 158 117 127 78 tfor:TF0638|3end_GTP-binding_protein_LepA_677283..677433_POSITIVE 247 160 116 139 100 tfor:TF1623|3end_5-formyltetrahydrofolate_cyclo-ligase_1737332..1737482_POSITIVE 247 103 62 46 5 tfor:TF1600|3end_uroporphyrinogen-III_synthase_1714817..1714967_POSITIVE 243 149 103 104 54 tfor:TF1983|3end_conserved_hypothetical_protein_2135000..2135150_POSITIVE 241 149 114 46 1 tfor:TF2594|3end_hypothetical_protein_2765125..2765275_POSITIVE