Origin IGS: aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtgcaacctaaattttaacgggacaaaggtaatattttttcgggaaacccgcggccacttccgcccttgtaacgggctgcttatggagtatgccttccccccgcgacgatacgattccgttattttttgcatcatctcaaacagctcttaaacggttgccgcatcttgtttgagcaatttaagtgttgtgaaaaggctcccgcattttgttttaacattttaagcgttgtgaaaaggctcgcacaaattgttttaacattccaagcgttctaaaaaggccgccacactttgttttaacattccaagcatt Mask Tandem Repeat Region ================================================ aatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Find is-nt database================================================ Query_seq: TF2653:TF2654|TF2653:TF2654:hypothetical protein:arginyl-tRNA synthetase:->->:2822521..2822831 311 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2653:TF2654|TF2653:TF2654:hypothetical protein:arginyl-tRNA synthetase:->->:2822521..2822831 311 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2653:TF2654|TF2653:TF2654:hypothetical protein:arginyl-tRNA synthetase:->->:2822521..2822831 311 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 63 End: 176 .............................................................. M C A S L F T T L K M L K Q N A G A F S Q H L N C S N K M R Q P F K S C L R ....................................................................................................................................... Protein_Len: 35 Strand: + Start: 196 End: 300 ................................................................................................................................................................................................... M V S S R G E G I L H K Q P V T R A E V A A G F P K K Y Y L C P V K I ........... Protein_Len: 48 Strand: + Start: 167 End: 310 ...................................................................................................................................................................... M F E M M Q K I T E S Y R R G G K A Y S I S S P L Q G R K W P R V S R K N I T F V P L K F R L H . Protein_Len: 39 Strand: - Start: 163 End: 279 .................................................................................................................................................................. S S N S I I C F I V S D Y R R P P F A Y E M L L G N C P R F H G R T E R F F M ................................ Protein_Len: 54 Strand: - Start: 41 End: 202 ........................................ F A Q F T L V I Q A L R K V V S L I N F C F A P A K E C C K F Q E F L I R C G N L L Q K L H H L F Y R F R M ............................................................................................................. aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2653:TF2654|TF2653:TF2654:hypothetical protein:arginyl-tRNA synthetase:->->:2822521..2822831 311 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Intra-Species Hit: Count: 1 Min: 1 Max: 311 Len: 311 Subject: tfor_TF2653_TF2654|hypothetical protein:arginyl-tRNA synthetase|POSITIVE:POSITIVE|[2822521,2822831]|311 HSP 1 e-value: 1.0E-175 bit: 617.0 Len: 311 Query Start:1 Query End:311 Subject Strand: POSITIVE Subject Start: 1 Subject End: 311 aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca aatgcttggaatgttaaaacaaagtgtggcggcctttttagaacgcttggaatgttaaaacaatttgtgcgagccttttcacaacgcttaaaatgttaaaacaaaatgcgggagccttttcacaacacttaaattgctcaaacaagatgcggcaaccgtttaagagctgtttgagatgatgcaaaaaataacggaatcgtatcgtcgcggggggaaggcatactccataagcagcccgttacaagggcggaagtggccgcgggtttcccgaaaaaatattacctttgtcccgttaaaatttaggttgcaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGCUUGGAAUGUUAAAACAAAGUGUGGCGGCCUUUUUAGAACGCUUGGAAUGUUAAAACAAUUUGUGCGAGCCUUUUCACAACGCUUAAAAUGUUAAAACAAAAUGCGGGAGCCUUUUCACAACACUUAAAUUGCUCAAACAAGAUGCGGCAACCGUUUAAGAGCUGUUUGAGAUGAUGCAAAAAAUAACGGAAUCGUAUCGUCGCGGGGGGAAGGCAUACUCCAUAAGCAGCCCGUUACAAGGGCGGAAGUGGCCGCGGGUUUCCCGAAAAAAUAUUACCUUUGUCCCGUUAAAAUUUAGGUUGCACA ...(((((((...((((.((((((.(((((((((.((((.....(((((((.((((..((((.((((.(((.............))).)))).))))..))))..........(((((..(.......((((((.((((.....(((((.(....))))))..)))).))))))((((((((................)))))))).....)..)))))....))))..))).((((.......)))).)))).))))))(((...)))..........)))))))))....))))..)))))))...... (-81.31) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGCAACCUAAAUUUUAACGGGACAAAGGUAAUAUUUUUUCGGGAAACCCGCGGCCACUUCCGCCCUUGUAACGGGCUGCUUAUGGAGUAUGCCUUCCCCCCGCGACGAUACGAUUCCGUUAUUUUUUGCAUCAUCUCAAACAGCUCUUAAACGGUUGCCGCAUCUUGUUUGAGCAAUUUAAGUGUUGUGAAAAGGCUCCCGCAUUUUGUUUUAACAUUUUAAGCGUUGUGAAAAGGCUCGCACAAAUUGUUUUAACAUUCCAAGCGUUCUAAAAAGGCCGCCACACUUUGUUUUAACAUUCCAAGCAUU .((((..((...........))(((((((((...........((....)).((((((......((((.......)))).(((..((((((..(((((......((((.((.(((....)))...)).))))..........(((((.((((((..((((..(((...(((..((((..((((.......))))...))))..)))...)))..))))..)))))).)))))...)))))..(((.....)))......)))))))))..........)))))).)).)))))))............)))). (-76.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 248 203 153 109 tfor:TF0473|5end_cation_or_acridine_efflux_membrane-fusion_protein_491047..491277_POSITIVE 425 249 202 71 29 tfor:TF1294|5end_conserved_hypothetical_protein_1377142..1377372_POSITIVE 413 271 231 193 139 tfor:TF1928|5end_conserved_hypothetical_protein_2075529..2075759_POSITIVE 405 239 191 119 76 tfor:TF2296|5end_hypothetical_protein_2448892..2449122_POSITIVE 404 240 191 226 162 tfor:TF2079|5end_permease_2245585..2245815_POSITIVE 402 259 211 151 82 tfor:TF0842|5end_long-chain-fatty-acid--CoA_ligase_893053..893283_POSITIVE 400 240 192 203 160 tfor:TF0525|5end_conserved_hypothetical_protein_549265..549495_POSITIVE 395 269 222 213 157 tfor:TF1831|5end_conserved_hypothetical_protein_1980365..1980595_POSITIVE 391 270 224 197 140 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 389 262 231 228 189 tfor:TF1395|5end_signal_peptidase_I_1468266..1468496_POSITIVE 385 271 234 164 125 tfor:TF2788|5end_hypothetical_protein_2972201..2972431_POSITIVE 384 250 204 204 151 tfor:TF0529|5end_transposase_554776..555006_POSITIVE 378 248 203 149 109 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 378 260 211 90 31 tfor:TF0983|5end_nitroreductase_family_protein,__possible_NADH_dehydrogenase/NAD(P)H_nitroreductase_1039394..1039624_POSITIVE 377 250 204 206 152 tfor:TF1586|5end_hypothetical_protein_1690971..1691201_POSITIVE 374 249 203 199 149 tfor:TF0866|5end_naphthoate_synthase_916725..916955_POSITIVE 373 262 223 179 145 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 372 240 191 215 161 tfor:TF2879|5end_tRNA_(guanine-N-1)-methyltransferase_3068254..3068484_POSITIVE 372 249 203 152 93 tfor:TF0463|5end_precorrin-6Y_C5,15-methyltransferase_479015..479245_POSITIVE 371 270 222 166 110 tfor:TF2187|5end_conserved_hypothetical_protein_2361693..2361923_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 466 248 203 83 39 tfor:TF0472|3end_multidrug_resistance_protein,_AcrB/AcrD/AcrF_family_(cation_efflux_pump)_491057..491207_POSITIVE 425 249 202 105 63 tfor:TF1295|3end_conserved_hypothetical_protein_1377256..1377406_POSITIVE 395 272 232 120 64 tfor:TF0419|3end_conserved_hypothetical_protein_435794..435944_POSITIVE 385 271 234 141 102 tfor:TF2787|3end_30S_ribosomal_protein_S1_2972258..2972408_POSITIVE 384 250 204 127 74 tfor:TF0528|3end_two-component_system_sensor_histidine_kinase_554779..554929_POSITIVE 380 269 228 79 28 tfor:TF3000|3end_hypothetical_protein_3216740..3216890_POSITIVE 378 260 211 90 31 tfor:TF0982|3end_conserved_hypothetical_protein_1039474..1039624_POSITIVE 373 262 223 102 68 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 367 239 192 145 95 tfor:TF0242|3end_conserved_hypothetical_protein_274069..274219_POSITIVE 365 270 225 143 92 tfor:TF0991|3end_probable_NAD(FAD)-dependent_dehydrogenase_1048334..1048484_POSITIVE 364 219 190 41 4 tfor:TF2743|3end_conserved_hypothetical_protein_2926322..2926472_POSITIVE 355 239 196 104 38 tfor:TF2188|3end_conserved_hypothetical_protein_2363535..2363685_POSITIVE 355 238 197 140 88 tfor:TF0462|3end_possible_cobalamin_biosynthesis_precorrin-3_methylase_479095..479245_POSITIVE 351 261 223 36 2 tfor:TF2374|3end_UDP-3-O-(R-3-hydoxymyristoyl)-glucosamine-N-acylt_ransferase_2540892..2541042_POSITIVE 350 250 201 57 12 tfor:TF1255|3end_conserved_hypothetical_protein_1338151..1338301_POSITIVE 348 260 211 113 50 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 348 270 223 64 11 tfor:TF0537|3end_conserved_hypothetical_protein_566836..566986_POSITIVE 347 260 211 74 22 tfor:TF0993|3end_hypothetical_protein_1048782..1048932_POSITIVE 346 270 224 53 5 tfor:TF1899|3end_anti-sigma_factor_2053815..2053965_POSITIVE 345 239 191 66 4 tfor:TF0023|3end_possible_response_element_in_two_component_regulation_20233..20383_POSITIVE