Origin IGS: tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attatttttaaatgtttatttattcggtttaacgtttcttttaatgtcgcaaaagtacgatatagaaagtggataaacaaatatattgcagaaatttttccgtaatcactacaggatatctctaaacactaataaaaagtgatttacacccattcatccacttacaaatcctctatctatagaggcataagtaaggacactctttacca Mask Tandem Repeat Region ================================================ tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Find is-nt database================================================ Query_seq: TF2647:TF2648|TF2647:TF2648:transcriptional regulator, possible arylsulfatase regulator:conserved hypothetical protein:->->:2818196..2818404 209 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2647:TF2648|TF2647:TF2648:transcriptional regulator, possible arylsulfatase regulator:conserved hypothetical protein:->->:2818196..2818404 209 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2647:TF2648|TF2647:TF2648:transcriptional regulator, possible arylsulfatase regulator:conserved hypothetical protein:->->:2818196..2818404 209 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 91 End: 207 .......................................................................................... M S C S D Y G K I S A I Y L F I H F L Y R T F A T L K E T L N R I N K H L K I .. Protein_Len: 34 Strand: - Start: 108 End: 209 ........................................................................................................... P F I E A I Y K N I W K R Y R V K A V N F S V N F R I F L C K F I M tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2647:TF2648|TF2647:TF2648:transcriptional regulator, possible arylsulfatase regulator:conserved hypothetical protein:->->:2818196..2818404 209 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Intra-Species Hit: Count: 1 Min: 1 Max: 209 Len: 209 Subject: tfor_TF2647_TF2648|transcriptional regulator, possible arylsulfatase regulator:conserved hypothetical protein|POSITIVE:POSITIVE|[2818196,2818404]|209 HSP 1 e-value: 1.0E-115 bit: 414.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat tggtaaagagtgtccttacttatgcctctatagatagaggatttgtaagtggatgaatgggtgtaaatcactttttattagtgtttagagatatcctgtagtgattacggaaaaatttctgcaatatatttgtttatccactttctatatcgtacttttgcgacattaaaagaaacgttaaaccgaataaataaacatttaaaaataat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUAAAGAGUGUCCUUACUUAUGCCUCUAUAGAUAGAGGAUUUGUAAGUGGAUGAAUGGGUGUAAAUCACUUUUUAUUAGUGUUUAGAGAUAUCCUGUAGUGAUUACGGAAAAAUUUCUGCAAUAUAUUUGUUUAUCCACUUUCUAUAUCGUACUUUUGCGACAUUAAAAGAAACGUUAAACCGAAUAAAUAAACAUUUAAAAAUAAU .......((((((.(((((..((.((((((....))))))))..))))).((((((((..(((((...((((.......))))..(((((((.(((.((((...)))))))...)))))))...)))))..)))))))).((((..((.(((((....))))).))..))))......................))))))......... (-46.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAUUUUUAAAUGUUUAUUUAUUCGGUUUAACGUUUCUUUUAAUGUCGCAAAAGUACGAUAUAGAAAGUGGAUAAACAAAUAUAUUGCAGAAAUUUUUCCGUAAUCACUACAGGAUAUCUCUAAACACUAAUAAAAAGUGAUUUACACCCAUUCAUCCACUUACAAAUCCUCUAUCUAUAGAGGCAUAAGUAAGGACACUCUUUACCA (((((((((...((((((((((..((......))...(((((.((((((........)))))).)))))))))))))))..........(((.((...((((((.....))).))).)).)))...........))))))))).................(((((..((((((((....)))))).))..))))).............. (-34.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 54 12 206 156 tfor:TF0530|5end_hypothetical_protein_with_TPR_domain_556072..556302_POSITIVE 304 49 1 114 63 tfor:TF1312|5end_hypothetical_protein_1388465..1388695_POSITIVE 304 49 1 114 63 tfor:TF1311|5end_hypothetical_protein_1388242..1388472_POSITIVE 269 60 18 57 14 tfor:TF1113|5end_possible_transposase_1183767..1183997_POSITIVE 267 163 138 50 23 tfor:TF1691|5end_integrase_1809422..1809652_POSITIVE 265 68 21 92 40 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 254 64 24 212 163 tfor:TF0353|5end_transposase_element_371396..371626_POSITIVE 253 166 122 91 43 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 249 49 1 114 63 tfor:TF1310|5end_hypothetical_protein_1388019..1388249_POSITIVE 249 63 21 126 80 tfor:TF0062|5end_conserved_hypothetical_protein_64916..65146_POSITIVE 248 63 23 180 124 tfor:TF0514|5end_conserved_hypothetical_protein_538482..538712_POSITIVE 247 60 11 97 42 tfor:TF1933|5end_methionyl-tRNA_synthetase_2079675..2079905_POSITIVE 246 71 37 209 172 tfor:TF2359|5end_transposase_2525642..2525872_POSITIVE 245 60 11 98 50 tfor:TF0264|5end_ISPg3_(IS195)_transposase_292420..292650_POSITIVE 244 70 23 227 172 tfor:TF1504|5end_conserved_hypothetical_protein_1607211..1607441_POSITIVE 243 162 121 152 103 tfor:TF0639|5end_hypothetical_protein_677877..678107_POSITIVE 242 64 28 66 26 tfor:TF1549|5end_conserved_hypothetical_protein_1653993..1654223_POSITIVE 241 64 24 124 80 tfor:TF3068|5end_flavodoxin_A_3292812..3293042_POSITIVE 240 76 32 179 126 tfor:TF0773|5end_outer_membrane_efflux_protein_821218..821448_POSITIVE 239 64 22 83 36 tfor:TF2680|5end_conserved_hypothetical_protein_2855196..2855426_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 49 1 127 76 tfor:TF1311|3end_hypothetical_protein_1388558..1388708_POSITIVE 304 49 1 127 76 tfor:TF1310|3end_hypothetical_protein_1388335..1388485_POSITIVE 293 79 32 92 48 tfor:TF1985|3end_surface_antigen_BspA_2136905..2137055_POSITIVE 255 186 143 91 51 tfor:TF0751|3end_dipeptidyl_peptidase_IV_800830..800980_POSITIVE 253 122 86 146 104 tfor:TF2463|3end_anti-sigma_factor_2643064..2643214_POSITIVE 251 68 23 78 33 tfor:TF1797|3end_SNF_family_transporter_1938571..1938721_POSITIVE 249 49 1 127 76 tfor:TF1312|3end_hypothetical_protein_1388781..1388931_POSITIVE 249 63 21 136 90 tfor:TF0061|3end_hypothetical_protein_65006..65156_POSITIVE 248 185 143 119 66 tfor:TF1164|3end_two-component_system_sensor_histidine_kinase_1244778..1244928_POSITIVE 248 63 23 117 61 tfor:TF0513|3end_ferredoxin_538499..538649_POSITIVE 247 60 11 97 42 tfor:TF1932|3end_pigmentation_and_extracellular_proteinase_regulator_2079755..2079905_POSITIVE 241 64 24 109 65 tfor:TF3067|3end_conserved_hypothetical_protein_3292877..3293027_POSITIVE 240 76 32 78 25 tfor:TF0772|3end_conserved_hypothetical_protein_821197..821347_POSITIVE 232 146 108 146 106 tfor:TF0779|3end_possible_outer_membrane_protein_831874..832024_POSITIVE 232 60 14 137 102 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 228 186 141 91 49 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 227 56 21 149 108 tfor:TF2742|3end_single-strand_DNA-specific_exonuclease_2925603..2925753_POSITIVE 227 86 43 82 42 tfor:TF1788|3end_conserved_hypothetical_protein_1930119..1930269_POSITIVE 226 61 22 64 35 tfor:TF0239|3end_conserved_hypothetical_protein_270323..270473_POSITIVE 226 111 75 62 26 tfor:TF0047|3end_hypothetical_protein_54109..54259_POSITIVE