Origin IGS: tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctttgaaataaataaataatttgttcgtacaaaggaagcccttttccggcatttaaaaaatccctatttgtagggatttttccgaataaccgctctacgaaactgaatttccggacgagaatca Mask Tandem Repeat Region ================================================ tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Find is-nt database================================================ Query_seq: TF2636:TF2637|TF2636:TF2637:glutaminase:conserved hypothetical protein:->->:2807160..2807283 124 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2636:TF2637|TF2636:TF2637:glutaminase:conserved hypothetical protein:->->:2807160..2807283 124 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2636:TF2637|TF2636:TF2637:glutaminase:conserved hypothetical protein:->->:2807160..2807283 124 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 3 End: 122 .. M L V R K F S F V E R L F G K I P T N R D F L N A G K G L P L Y E Q I I Y L F Q .. Protein_Len: 36 Strand: - Start: 2 End: 109 . I R T R F N L K T S R N N P F I G V F L S K K F A P F P S G K Y S C I M ............... tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 95 HP_score: -7.6 Tail_Score: -5.08824 Start: 49 End: 64 Full_Region: GGTTATTCGGAAAAA TCCCTA CAAA TAGGGA TTTTTTAAATGCCGG ................................................TCCCTACAAATAGGGA............................................................ TransTerm Strand: - Conf: 86 HP_score: -7.6 Tail_Score: -4.59773 Start: 49 End: 64 Full_Region: ccggcatttaaaaaa tcccta tttg taggga tttttccgaataacc ................................................tccctatttgtaggga............................................................ TransTerm Strand: + Conf: 51 HP_score: -2.4 Tail_Score: -3.85817 Start: 78 End: 91 Full_Region: GATTTTTTAAATGCC GGAA AAGGGC TTCC TTTGTACGAACAAAT .............................................................................GGAAAAGGGCTTCC................................. Find igs database================================================ Query_seq: TF2636:TF2637|TF2636:TF2637:glutaminase:conserved hypothetical protein:->->:2807160..2807283 124 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Intra-Species Hit: Count: 1 Min: 1 Max: 124 Len: 124 Subject: tfor_TF2636_TF2637|glutaminase:conserved hypothetical protein|POSITIVE:POSITIVE|[2807160,2807283]|124 HSP 1 e-value: 1.0E-64 bit: 246.0 Len: 124 Query Start:1 Query End:124 Subject Strand: POSITIVE Subject Start: 1 Subject End: 124 tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag tgattctcgtccggaaattcagtttcgtagagcggttattcggaaaaatccctacaaatagggattttttaaatgccggaaaagggcttcctttgtacgaacaaattatttatttatttcaaag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUCUCGUCCGGAAAUUCAGUUUCGUAGAGCGGUUAUUCGGAAAAAUCCCUACAAAUAGGGAUUUUUUAAAUGCCGGAAAAGGGCUUCCUUUGUACGAACAAAUUAUUUAUUUAUUUCAAAG (((((((.....))))..)))..((((((...((((......((((((((((((....))))))))))))....))))..(((((....))))).))))))....................... (-28.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUGAAAUAAAUAAAUAAUUUGUUCGUACAAAGGAAGCCCUUUUCCGGCAUUUAAAAAAUCCCUAUUUGUAGGGAUUUUUCCGAAUAACCGCUCUACGAAACUGAAUUUCCGGACGAGAAUCA (((((.(((((((.....)))))))....)))))......(((.(((((.((((.(((((((((((....)))))))))))..))))...((.....))...........))))).)))..... (-26.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 344 43 6 151 116 tfor:TF2231|5end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_2400307..2400537_POSITIVE 323 43 4 196 155 tfor:TF0431|5end_hypothetical_protein_452178..452408_POSITIVE 323 43 4 181 140 tfor:TF0430|5end_hypothetical_protein_452163..452393_POSITIVE 322 50 3 203 151 tfor:TF0212|5end_DNA/pantothenate_metabolism_flavoprotein_237719..237949_POSITIVE 320 42 1 89 55 tfor:TF2004|5end_thymidylate_synthase_2156372..2156602_POSITIVE 315 42 8 192 156 tfor:TF0765|5end_conserved_hypothetical_protein_811302..811532_POSITIVE 304 50 6 73 14 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 299 42 3 152 121 tfor:TF1985|5end_surface_antigen_BspA_2135659..2135889_POSITIVE 294 52 11 147 105 tfor:TF1507|5end_outer_membrane_receptor,_TonB-dependent_1614536..1614766_POSITIVE 293 44 5 57 13 tfor:TF2916|5end_conserved_hypothetical_protein_3111559..3111789_POSITIVE 283 45 1 86 36 tfor:TF2697|5end_small_heat_shock_protein_2871678..2871908_POSITIVE 276 56 11 220 173 tfor:TF0786|5end_pyridoxal_phosphate_biosynthetic_protein_838563..838793_POSITIVE 268 45 5 218 169 tfor:TF2314|5end_conserved_hypothetical_protein_2473530..2473760_POSITIVE 266 34 4 222 188 tfor:TF2779|5end_fucose_permease_2964566..2964796_POSITIVE 266 99 51 222 171 tfor:TF2332|5end_signal_peptidase_I_2491694..2491924_POSITIVE 266 46 6 65 17 tfor:TF1624|5end_carboxyl-terminal_protease_1737252..1737482_POSITIVE 264 45 9 50 11 tfor:TF1946|5end_membrane_protein;_spore_maturation-like_protein_2094289..2094519_POSITIVE 264 34 3 168 134 tfor:TF1334|5end_hypothetical_protein_1415996..1416226_POSITIVE 264 43 8 173 142 tfor:TF0242|5end_conserved_hypothetical_protein_272439..272669_POSITIVE 263 42 6 213 158 tfor:TF2533|5end_adenylosuccinate_synthetase_2715666..2715896_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 50 3 150 98 tfor:TF0211|3end_DNA_polymerase_III,_epsilon_subunit_237746..237896_POSITIVE 320 42 1 64 30 tfor:TF2003|3end_dihydrofolate_reductase_2156427..2156577_POSITIVE 319 80 31 52 3 tfor:TF2636|3end_glutaminase_2807099..2807249_POSITIVE 304 50 6 73 14 tfor:TF2519|3end_membrane_protein,_metallopeptidase_M23/M37_family_2698997..2699147_POSITIVE 286 43 1 143 93 tfor:TF2786|3end_hypothetical_protein_2970302..2970452_POSITIVE 276 85 41 69 16 tfor:TF2693|3end_possible_aminotransferase_2869720..2869870_POSITIVE 270 43 5 139 105 tfor:TF2768|3end_ferredoxin_oxidoreductase,_beta_subunit_2955169..2955319_POSITIVE 266 46 6 65 17 tfor:TF1623|3end_5-formyltetrahydrofolate_cyclo-ligase_1737332..1737482_POSITIVE 264 34 3 106 72 tfor:TF1333|3end_probable_acylaminoacyl_peptidase;_alanyl_dipeptidyl_peptidase_1416014..1416164_POSITIVE 264 43 8 149 118 tfor:TF0241|3end_UDP-N-acetyl_glucosamine_-2-epimerase_272495..272645_POSITIVE 261 99 58 149 97 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE 260 42 6 151 123 tfor:TF2230|3end_CTn_excision_protein_2400394..2400544_POSITIVE 259 53 11 67 41 tfor:TF1479|3end_ABC_transporter,_permease_component_1573592..1573742_POSITIVE 257 65 23 79 35 tfor:TF2511|3end_RNA_binding_protein_2690232..2690382_POSITIVE 257 45 11 46 17 tfor:TF0358|3end_hypothetical_protein_376304..376454_POSITIVE 255 98 60 58 19 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 253 68 23 150 100 tfor:TF2281|3end_hypothetical_protein_2439995..2440145_POSITIVE 252 59 11 108 52 tfor:TF2392|3end_alpha-galactosidase_2565506..2565656_POSITIVE 252 42 7 88 46 tfor:TF1326|3end_hypothetical_protein_1404100..1404250_POSITIVE 251 78 33 49 6 tfor:TF2422|3end_hypothetical_protein_2601677..2601827_POSITIVE