Origin IGS: taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaattaaattcattctcattggttctattgcggaatccggattgaatgtataagcccaaggttgcaaatcgtctataaaaaaagctgtctcgttttccgaaacagctcttgtaggatttaatcatctacttgtgcttta Mask Tandem Repeat Region ================================================ taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Find is-nt database================================================ Query_seq: TF2546:TF2547|TF2546:TF2547:S-adenosylmethionine synthetase:hypothetical protein:->->:2730664..2730803 140 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2546:TF2547|TF2546:TF2547:S-adenosylmethionine synthetase:hypothetical protein:->->:2730664..2730803 140 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2546:TF2547|TF2546:TF2547:S-adenosylmethionine synthetase:hypothetical protein:->->:2730664..2730803 140 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 15 End: 140 .............. M I K S Y K S C F G K R D S F F Y R R F A T L G L Y I Q S G F R N R T N E N E F N S Protein_Len: 37 Strand: - Start: 22 End: 132 ..................... I R C S S N R F V L C S K K Y V I Q L R P S I C E I R I G C Y F W H S H M ........ taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 56 HP_score: -5.1 Tail_Score: -3.40382 Start: 32 End: 58 Full_Region: aatcgtctataaaaa aagctgtctcg ttttc cgaaacagctc ttgtaggatttaatc ...............................aagctgtctcgttttccgaaacagctc.................................................................................. Find igs database================================================ Query_seq: TF2546:TF2547|TF2546:TF2547:S-adenosylmethionine synthetase:hypothetical protein:->->:2730664..2730803 140 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Intra-Species Hit: Count: 1 Min: 1 Max: 140 Len: 140 Subject: tfor_TF2546_TF2547|S-adenosylmethionine synthetase:hypothetical protein|POSITIVE:POSITIVE|[2730664,2730803]|140 HSP 1 e-value: 2.0E-61 bit: 236.0 Len: 140 Query Start:1 Query End:140 Subject Strand: POSITIVE Subject Start: 1 Subject End: 140 taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagcnnnnnnnatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca taaagcacaagtagatgattaaatcctacaagagctgtttcggaaaacgagacagctttttttatagacgatttgcaaccttgggcttatacattcaatccggattccgcaatagaaccaatgagaatgaatttaattca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCACAAGUAGAUGAUUAAAUCCUACAAGAGCUGUUUCGGAAAACGAGACAGCUUUUUUUAUAGACGAUUUGCAACCUUGGGCUUAUACAUUCAAUCCGGAUUCCGCAAUAGAACCAAUGAGAAUGAAUUUAAUUCA ..((((.((((....((..(((((((((.(((((((((((((.....)))))))))))))....)))..))))))))..)))).))))...(((((.....((.(((.......))))).....)))))........... (-32.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUUAAAUUCAUUCUCAUUGGUUCUAUUGCGGAAUCCGGAUUGAAUGUAUAAGCCCAAGGUUGCAAAUCGUCUAUAAAAAAAGCUGUCUCGUUUUCCGAAACAGCUCUUGUAGGAUUUAAUCAUCUACUUGUGCUUUA ........(((((.(((....((((((.....)))))).))).)))))....((((.((((..((.(((((..((((((....((((((.(((.....))).)))))).)))))))))))...))....)))).)))).. (-31.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 538 140 97 166 123 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 538 140 97 141 98 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 513 140 97 148 105 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 441 140 97 206 163 tfor:TF1474|5end_6-phosphogluconolactonase_1567342..1567572_POSITIVE 420 138 97 231 190 tfor:TF3004|5end_phosphoribosylformylglycinamidine_(FGAM)_synthase_3220819..3221049_POSITIVE 383 63 23 160 104 tfor:TF0334|5end_hypothetical_protein_360043..360273_POSITIVE 340 140 97 211 175 tfor:TF2204|5end_conserved_hypothetical_protein_2378866..2379096_POSITIVE 320 109 61 65 12 tfor:TF0635|5end_glucosylceramidase_precursor_669221..669451_POSITIVE 312 110 68 206 162 tfor:TF2565|5end_50S_ribosomal_protein_L24_2739986..2740216_POSITIVE 300 59 25 125 90 tfor:TF2407|5end_glycosyltransferase_2581574..2581804_POSITIVE 295 117 79 136 98 tfor:TF2351|5end_acetate_kinase_2515480..2515710_POSITIVE 289 85 41 64 18 tfor:TF0553|5end_Bacteroides_aerotolerance_protein,_BatD_584462..584692_POSITIVE 278 87 48 192 152 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 275 110 64 63 7 tfor:TF2673|5end_conserved_hypothetical_protein_2846987..2847217_POSITIVE 275 82 41 218 169 tfor:TF0923|5end_tetracycline_resistance_element_mobilization_regulatory_protein_961256..961486_POSITIVE 275 90 42 190 134 tfor:TF0839|5end_aminopeptidasem_M28_family_890442..890672_POSITIVE 273 120 71 55 20 tfor:TF1221|5end_conserved_hypothetical_protein_1307723..1307953_POSITIVE 269 110 69 185 136 tfor:TF2835|5end_stationary-phase_survival_protein_SurE_3025113..3025343_POSITIVE 269 113 76 76 41 tfor:TF1204|5end_possible_metal-dependent_membrane_protease_1286359..1286589_POSITIVE 267 110 62 150 99 tfor:TF1295|5end_conserved_hypothetical_protein_1375854..1376084_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 538 140 97 90 47 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 383 63 23 82 26 tfor:TF0332|3end_conserved_hypothetical_protein_360045..360195_POSITIVE 339 60 21 61 17 tfor:TF2546|3end_S-adenosylmethionine_synthetase_2730603..2730753_POSITIVE 300 59 25 130 95 tfor:TF2406|3end_anti-sigma_factor_2581659..2581809_POSITIVE 286 71 31 71 34 tfor:TF2349|3end_conserved_hypothetical_protein_2514921..2515071_POSITIVE 278 87 48 46 6 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 273 120 71 49 14 tfor:TF1220|3end_conserved_hypothetical_protein_1307797..1307947_POSITIVE 272 62 31 140 110 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 267 110 62 140 89 tfor:TF1293|3end_ATPase,_possible_AAA_superfamily_1375924..1376074_POSITIVE 267 63 30 71 38 tfor:TF0548|3end_TPR-repeat-containing_protein_581417..581567_POSITIVE 266 83 43 93 54 tfor:TF0189|3end_phosphatidate_cytidylytransferase_(CDP-diglyceride_synthetase)_204090..204240_POSITIVE 260 128 83 79 29 tfor:TF0169|3end_possible_CRISPR-associated_protein_Cas1_179643..179793_POSITIVE 259 110 64 130 79 tfor:TF2373|3end_UDP-3-O-acyl-GlcNAc_deacetylase_2539806..2539956_POSITIVE 258 110 66 149 105 tfor:TF2876|3end_cytosine_deaminase_3065664..3065814_POSITIVE 252 117 73 59 14 tfor:TF1079|3end_transposase_1149769..1149919_POSITIVE 249 87 48 84 44 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 243 117 97 59 39 tfor:TF2952|3end_transposase_3160989..3161139_POSITIVE 242 75 31 68 33 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 238 119 79 67 17 tfor:TF2852|3end_conserved_hypothetical_protein_3040221..3040371_POSITIVE 238 100 54 127 77 tfor:TF1626|3end_nicotinate-nucleotide_adenylyltransferase_1740098..1740248_POSITIVE