Origin IGS: taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttttcgccgggtgcgtctttaataaatcaccccaataggctttataatttattatgatttatgttgataaaggaagtttctgatgccatgtttatgtggcttcttgaagggcttgttgcagaggtcaatggttgttgcacctgaatatttggggatatgtctgtagggaagactcagattgaagaatcgttctttttaatggctggactcttgagccttttattaccgagagaagttcctttaatattatttagtcctgcatttatttgcgccgtttgcgttcggatatcgtcattcgtaattgatcttagccgtcagcattcagccaacaattcaacaaatcaacgcccgaaaggcttcgcttttcacgattcacttttcgtttccactaatatagattcacaacctatgtctttttcggagtagaaaagaagaagggagaagtctgttaaatcacaatataatcaatagcttacagaaaatattgtgtttgtgtgagtatgttgatgctgttactgcaaagggcagccaaggatactctccctgactgccttttaaggttgattatgcctttttta Mask Tandem Repeat Region ================================================ taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Find is-nt database================================================ Query_seq: TF2482:TF2483|TF2482:TF2483:hypothetical protein:hypothetical protein:->->:2659246..2659824 579 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2482:TF2483|TF2482:TF2483:hypothetical protein:hypothetical protein:->->:2659246..2659824 579 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2482:TF2483|TF2482:TF2483:hypothetical protein:hypothetical protein:->->:2659246..2659824 579 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 11 End: 103 .......... M I N L K R Q S G R V S L A A L C S N S I N I L T Q T Q Y F L ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 42 Strand: + Start: 277 End: 402 .................................................................................................................................................................................................................................................................................... M T N D D I R T Q T A Q I N A G L N N I K G T S L G N K R L K S P A I K K N D S S I ................................................................................................................................................................................. Protein_Len: 41 Strand: + Start: 329 End: 451 ........................................................................................................................................................................................................................................................................................................................................ M I L K E L L S V I K G S R V Q P L K R T I L Q S E S S L Q T Y P Q I F R C N N H ................................................................................................................................ Protein_Len: 41 Strand: + Start: 402 End: 524 ................................................................................................................................................................................................................................................................................................................................................................................................................. M S L P Y R H I P K Y S G A T T I D L C N K P F K K P H K H G I R N F L Y Q H K S ....................................................... Protein_Len: 38 Strand: + Start: 421 End: 534 .................................................................................................................................................................................................................................................................................................................................................................................................................................... M S P N I Q V Q Q P L T S A T S P S R S H I N M A S E T S F I N I N H N K L ............................................. Protein_Len: 31 Strand: - Start: 185 End: 277 ........................................................................................................................................................................................ I L P F S F H I T F L S A K R A N I Q Q I T P Q I S V A L I M .............................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 156 End: 434 ........................................................................................................................................................... S H Q R S L D I V F S S I R V C V A C I F A P S F L I L P V E R P L L L S L L G A M L F F S E E I Q T K G V S M D G F M ................................................................................................................................................. Protein_Len: 49 Strand: - Start: 35 End: 181 .................................. P S Y G Q S G K A T V A D V Y E C L C L I K Q L S N I I N H N L L S R G E E K R S R F L C L N H M .............................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 37 Strand: - Start: 1 End: 111 L F P M I L R L L C D P L T D K A A R Q L L L M L M S V C V C Y K R Y A M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 83 HP_score: -8.8 Tail_Score: -4.21768 Start: 27 End: 55 Full_Region: gctgttactgcaaag ggcagccaagga tactc tccctgactgcc ttttaaggttgatta ..........................ggcagccaaggatactctccctgactgcc............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: TF2482:TF2483|TF2482:TF2483:hypothetical protein:hypothetical protein:->->:2659246..2659824 579 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Intra-Species Hit: Count: 1 Min: 1 Max: 579 Len: 579 Subject: tfor_TF2482_TF2483|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2659246,2659824]|579 HSP 1 e-value: 0.0 bit: 1148.0 Len: 579 Query Start:1 Query End:579 Subject Strand: POSITIVE Subject Start: 1 Subject End: 579 taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac taaaaaaggcataatcaaccttaaaaggcagtcagggagagtatccttggctgccctttgcagtaacagcatcaacatactcacacaaacacaatattttctgtaagctattgattatattgtgatttaacagacttctcccttcttcttttctactccgaaaaagacataggttgtgaatctatattagtggaaacgaaaagtgaatcgtgaaaagcgaagcctttcgggcgttgatttgttgaattgttggctgaatgctgacggctaagatcaattacgaatgacgatatccgaacgcaaacggcgcaaataaatgcaggactaaataatattaaaggaacttctctcggtaataaaaggctcaagagtccagccattaaaaagaacgattcttcaatctgagtcttccctacagacatatccccaaatattcaggtgcaacaaccattgacctctgcaacaagcccttcaagaagccacataaacatggcatcagaaacttcctttatcaacataaatcataataaattataaagcctattggggtgatttattaaagacgcacccggcgaaaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAGGCAUAAUCAACCUUAAAAGGCAGUCAGGGAGAGUAUCCUUGGCUGCCCUUUGCAGUAACAGCAUCAACAUACUCACACAAACACAAUAUUUUCUGUAAGCUAUUGAUUAUAUUGUGAUUUAACAGACUUCUCCCUUCUUCUUUUCUACUCCGAAAAAGACAUAGGUUGUGAAUCUAUAUUAGUGGAAACGAAAAGUGAAUCGUGAAAAGCGAAGCCUUUCGGGCGUUGAUUUGUUGAAUUGUUGGCUGAAUGCUGACGGCUAAGAUCAAUUACGAAUGACGAUAUCCGAACGCAAACGGCGCAAAUAAAUGCAGGACUAAAUAAUAUUAAAGGAACUUCUCUCGGUAAUAAAAGGCUCAAGAGUCCAGCCAUUAAAAAGAACGAUUCUUCAAUCUGAGUCUUCCCUACAGACAUAUCCCCAAAUAUUCAGGUGCAACAACCAUUGACCUCUGCAACAAGCCCUUCAAGAAGCCACAUAAACAUGGCAUCAGAAACUUCCUUUAUCAACAUAAAUCAUAAUAAAUUAUAAAGCCUAUUGGGGUGAUUUAUUAAAGACGCACCCGGCGAAAAC ......((((.(((((((........((((((((((((.....)))))))))))).(((((((.....................................)))))))...)))))))((((((((((((.((((..((........(((((((......).))))))....((((((..(((((((((.(.(((..........(((((((.....(((......(((((..((((((((((..((((((((((((........)))))))...)))))))))))..))))..))))))))....((((((......))).(((((......(((((..(((.....)).)..)))))...........))))).)))..........))))))).....((((((......)).))))....))).).)))))..))))...))))))...))..))))...............((.((((........)))).))....................)))))))))))).........))))....(((((..((......))..)))))......... (-131.92) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUCGCCGGGUGCGUCUUUAAUAAAUCACCCCAAUAGGCUUUAUAAUUUAUUAUGAUUUAUGUUGAUAAAGGAAGUUUCUGAUGCCAUGUUUAUGUGGCUUCUUGAAGGGCUUGUUGCAGAGGUCAAUGGUUGUUGCACCUGAAUAUUUGGGGAUAUGUCUGUAGGGAAGACUCAGAUUGAAGAAUCGUUCUUUUUAAUGGCUGGACUCUUGAGCCUUUUAUUACCGAGAGAAGUUCCUUUAAUAUUAUUUAGUCCUGCAUUUAUUUGCGCCGUUUGCGUUCGGAUAUCGUCAUUCGUAAUUGAUCUUAGCCGUCAGCAUUCAGCCAACAAUUCAACAAAUCAACGCCCGAAAGGCUUCGCUUUUCACGAUUCACUUUUCGUUUCCACUAAUAUAGAUUCACAACCUAUGUCUUUUUCGGAGUAGAAAAGAAGAAGGGAGAAGUCUGUUAAAUCACAAUAUAAUCAAUAGCUUACAGAAAAUAUUGUGUUUGUGUGAGUAUGUUGAUGCUGUUACUGCAAAGGGCAGCCAAGGAUACUCUCCCUGACUGCCUUUUAAGGUUGAUUAUGCCUUUUUUA ........((((((((....((((.....(((((((((((((((....(((((((..........)))))))..........((.(((((......))))).))....)))))))))))..).))).....)))).))))))))......(((.(((((...(((((((.........((((((((((..(((((..((((((..((.((....))))..))))))..)))))..))).))))))).........))))))).......((((.....))))....))))).)))..((((((..((((((.......(((..(((((((((((((((((((.........(((((((...)))))))..(((..(((((((.((((.....((((((.........)))))).((((((......))))))..)))).)))))))..)))....((((((((..((...........))..))))))))))))).))))).)))))..))))....))).((((((((.((.(((.....))).)).))))))))))))))..))))))......... (-147.57) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 602 270 222 181 126 tfor:TF0359|5end_conserved_hypothetical_protein_376943..377173_POSITIVE 573 250 201 161 112 tfor:TF0347|5end_possible_serine_protease_367542..367772_POSITIVE 563 256 211 138 92 tfor:TF0367|5end_eukaryotic-like__metalloproteinase_384007..384237_POSITIVE 563 256 211 138 92 tfor:TF0364|5end_ysyl_endopeptidase_380395..380625_POSITIVE 558 250 201 163 114 tfor:TF1173|5end_ABC_transporter,_permease_component_1255444..1255674_POSITIVE 558 250 201 163 114 tfor:TF1172|5end_ABC_transporter,_permease_component_1252806..1253036_POSITIVE 496 247 201 142 89 tfor:TF0341|5end_conserved_hypothetical_protein_364348..364578_POSITIVE 487 269 222 198 126 tfor:TF0351|5end_hypothetical_protein_370922..371152_POSITIVE 461 239 191 149 99 tfor:TF2167|5end_hypothetical_protein_2342586..2342816_POSITIVE 449 260 211 160 115 tfor:TF2162|5end_thermolysin_precursor_2338669..2338899_POSITIVE 427 252 211 156 115 tfor:TF2174|5end_possible_endopeptidase_precursor_2346292..2346522_POSITIVE 423 269 229 63 18 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 413 245 211 231 197 tfor:TF0371|5end_beta-mannosidase_precursor_386763..386993_POSITIVE 412 320 276 172 114 tfor:TF2014|5end_dipeptidyl_aminopeptidase_IV_2169398..2169628_POSITIVE 411 325 282 81 35 tfor:TF0018|5end_hypothetical_protein_11250..11480_POSITIVE 408 269 222 178 138 tfor:TF1417|5end_conserved_hypothetical_protein_1499544..1499774_POSITIVE 407 252 213 218 181 tfor:TF2109|5end_possible_metallo-beta-lactamase_family_protein_2277942..2278172_POSITIVE 392 269 239 223 193 tfor:TF0725|5end_O-acetylhomoserine_(thiol)-lyase_776556..776786_POSITIVE 389 320 282 212 164 tfor:TF2996|5end_hypothetical_protein_3210802..3211032_POSITIVE 386 270 221 62 13 tfor:TF2045|5end_aminoacyl-histidine_dipeptidase_2206114..2206344_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 558 250 201 76 27 tfor:TF1170|3end_conserved_hypothetical_protein_1252799..1252949_POSITIVE 404 321 287 95 52 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 403 322 287 138 103 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 403 322 287 66 31 tfor:TF2173|3end_hypothetical_protein_2346063..2346213_POSITIVE 385 321 281 36 1 tfor:TF2013|3end_polysaccharide_deacetylase_2169341..2169491_POSITIVE 374 61 25 43 1 tfor:TF0930|3end_conserved_hypothetical_protein;_possible_prophage_protein_969965..970115_POSITIVE 373 55 27 64 36 tfor:TF2482|3end_hypothetical_protein_2659185..2659335_POSITIVE 372 273 238 80 43 tfor:TF1172|3end_ABC_transporter,_permease_component_1255213..1255363_POSITIVE 369 322 287 66 31 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 369 322 287 66 31 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 368 268 226 51 3 tfor:TF0960|3end_beta-galactosidase_1010506..1010656_POSITIVE 354 310 261 150 114 tfor:TF2780|3end_hypothetical_protein_2966526..2966676_POSITIVE 351 325 284 135 90 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 345 322 293 66 37 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 341 268 222 65 23 tfor:TF0593|3end_membrane-associated_phospholipid_phosphatase_627051..627201_POSITIVE 337 70 26 53 6 tfor:TF0881|3end_conserved_hypothetical_protein_935993..936143_POSITIVE 333 228 181 60 22 tfor:TF2657|3end_conserved_hypothetical_protein_2830873..2831023_POSITIVE 329 270 222 45 6 tfor:TF1474|3end_6-phosphogluconolactonase_1568123..1568273_POSITIVE 329 61 24 59 16 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 327 325 284 61 16 tfor:TF2996|3end_hypothetical_protein_3211121..3211271_POSITIVE