Origin IGS: taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatgatcgatgcatttgggtatggaaagaagaaaaaggaggatgagacaggaattcatcccccttttacggttagaagaatttttgcaactgcaaaatcatcctatagcctttcaaataaacgatccggaatgtgcatta Mask Tandem Repeat Region ================================================ taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Find is-nt database================================================ Query_seq: TF2421:TF2422|TF2421:TF2422:cytocidal toxin protein:hypothetical protein:->->:2601298..2601437 140 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2421:TF2422|TF2421:TF2422:cytocidal toxin protein:hypothetical protein:->->:2601298..2601437 140 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2421:TF2422|TF2421:TF2422:cytocidal toxin protein:hypothetical protein:->->:2601298..2601437 140 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: - Start: 2 End: 139 . I C M G S R K N S L S Y S S K A T A F I R R V T F P P H I G T E D E K K K K G Y G F A D I M . Protein_Len: 44 Strand: - Start: 1 End: 132 L A C E P D N I Q F A I P H N Q L Q L F E E L R L L P I F E Q R M R R K R R E M G L H M ........ taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 77 HP_score: -8.3 Tail_Score: -3.96216 Start: 72 End: 110 Full_Region: AAAATTCTTCTAACC GTAAAAGGGGGATGA ATTCCTGTC TCATCCTCCTTTTTC TTCTTTCCATACCCA .......................................................................GTAAAAGGGGGATGAATTCCTGTCTCATCCTCCTTTTTC.............................. TransTerm Strand: + Conf: 85 HP_score: -5.9 Tail_Score: -5.38549 Start: 78 End: 104 Full_Region: CTTCTAACCGTAAAA GGGGGATGA ATTCCTGTC TCATCCTCC TTTTTCTTCTTTCCA .............................................................................GGGGGATGAATTCCTGTCTCATCCTCC.................................... TransTerm Strand: - Conf: 61 HP_score: -4.4 Tail_Score: -4.19238 Start: 78 End: 104 Full_Region: tggaaagaagaaaaa ggaggatgag acaggaa ttcatccccc ttttacggttagaag .............................................................................ggaggatgagacaggaattcatccccc.................................... Find igs database================================================ Query_seq: TF2421:TF2422|TF2421:TF2422:cytocidal toxin protein:hypothetical protein:->->:2601298..2601437 140 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Intra-Species Hit: Count: 1 Min: 1 Max: 140 Len: 140 Subject: tfor_TF2421_TF2422|cytocidal toxin protein:hypothetical protein|POSITIVE:POSITIVE|[2601298,2601437]|140 HSP 1 e-value: 5.0E-74 bit: 278.0 Len: 140 Query Start:1 Query End:140 Subject Strand: POSITIVE Subject Start: 1 Subject End: 140 taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc taatgcacattccggatcgtttatttgaaaggctataggatgattttgcagttgcaaaaattcttctaaccgtaaaagggggatgaattcctgtctcatcctcctttttcttctttccatacccaaatgcatcgatcatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGCACAUUCCGGAUCGUUUAUUUGAAAGGCUAUAGGAUGAUUUUGCAGUUGCAAAAAUUCUUCUAACCGUAAAAGGGGGAUGAAUUCCUGUCUCAUCCUCCUUUUUCUUCUUUCCAUACCCAAAUGCAUCGAUCAUC ..............(((((..((((((...((.((.((((...((((((....))))))..)))).)).))..(((((((((((((.........)))))))))))))...............))))))...)))))... (-37.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGAUCGAUGCAUUUGGGUAUGGAAAGAAGAAAAAGGAGGAUGAGACAGGAAUUCAUCCCCCUUUUACGGUUAGAAGAAUUUUUGCAACUGCAAAAUCAUCCUAUAGCCUUUCAAAUAAACGAUCCGGAAUGUGCAUUA .......(((((((((((((.((.........((((((.(((((((.......))))))).))))))..(((((...((..((((((....))))))...))...)))))...........))))))))...))))))). (-37.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 416 130 86 203 153 tfor:TF2636|5end_glutaminase_2806096..2806326_POSITIVE 401 108 61 167 114 tfor:TF1740|5end_hypothetical_protein_1868645..1868875_POSITIVE 335 114 78 109 68 tfor:TF1598|5end_conserved_hypothetical_protein_1713358..1713588_POSITIVE 306 119 77 188 136 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 305 112 77 138 107 tfor:TF1470|5end_phosphohydrolase,_Icc_family_1563144..1563374_POSITIVE 299 55 11 161 118 tfor:TF0859|5end_conserved_hypothetical_protein_911753..911983_POSITIVE 293 105 61 146 100 tfor:TF2904|5end_ATP-dependent__helicase,_possible_UvrD/REP_helicase_domain_protein_3098903..3099133_POSITIVE 286 130 97 145 113 tfor:TF2172|5end_hypothetical_protein_2345822..2346052_POSITIVE 286 130 97 139 107 tfor:TF2173|5end_hypothetical_protein_2345816..2346046_POSITIVE 286 130 97 139 107 tfor:TF2171|5end_hypothetical_protein_2345485..2345715_POSITIVE 286 130 97 139 107 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 286 130 97 139 107 tfor:TF0366|5end_hypothetical_protein_383564..383794_POSITIVE 286 130 97 139 107 tfor:TF0362|5end_hypothetical_protein_379952..380182_POSITIVE 286 130 97 139 107 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 282 104 64 52 5 tfor:TF2878|5end_conserved_hypothetical_protein;_possible_CAAX_amino_terminal_protease_family_3067304..3067534_POSITIVE 282 87 44 157 116 tfor:TF2336|5end_conserved_hypothetical_protein_2496169..2496399_POSITIVE 282 130 97 139 107 tfor:TF0346|5end_hypothetical_protein_367048..367278_POSITIVE 281 49 3 170 125 tfor:TF0446|5end_hypothetical_protein_466473..466703_POSITIVE 280 110 61 148 91 tfor:TF2453|5end_glutamate_racemase_2630422..2630652_POSITIVE 279 55 13 78 42 tfor:TF3123|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_3357775..3358005_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 416 130 86 128 78 tfor:TF2635|3end_ABC_transporter,_ATP-binding_protein/permease_component_2806101..2806251_POSITIVE 335 114 78 89 48 tfor:TF1597|3end_TatD_family_protein_1713418..1713568_POSITIVE 316 120 74 55 12 tfor:TF1739|3end_possible_sulfatase_1868604..1868754_POSITIVE 306 119 77 128 76 tfor:TF2783|3end_aminotransferase,_PatB_family_2969297..2969447_POSITIVE 293 105 61 149 103 tfor:TF2903|3end_translation_initiation_factor_SUI1_3098986..3099136_POSITIVE 286 130 97 35 3 tfor:TF2171|3end_hypothetical_protein_2345792..2345942_POSITIVE 282 87 44 85 44 tfor:TF2335|3end_conserved_hypothetical_protein_2496177..2496327_POSITIVE 281 49 3 135 90 tfor:TF0444|3end_Na+-translocating_NADH-quinone_oxidoreductase,_subunit_5_466518..466668_POSITIVE 280 110 61 61 4 tfor:TF2452|3end_outer_membrane_protein,_OmpH_family_2630415..2630565_POSITIVE 274 105 71 83 38 tfor:TF0537|3end_conserved_hypothetical_protein_566836..566986_POSITIVE 271 69 21 64 13 tfor:TF0826|3end_primosomal_protein_N'_886290..886440_POSITIVE 270 39 2 92 48 tfor:TF0288|3end_conserved_hypothetical_protein_318099..318249_POSITIVE 262 55 12 129 81 tfor:TF1766|3end_alanyl-tRNA_synthetase_1905565..1905715_POSITIVE 260 44 3 92 56 tfor:TF2015|3end_N6-adenine-specific_DNA_methylase_2173020..2173170_POSITIVE 254 102 61 116 69 tfor:TF1624|3end_carboxyl-terminal_protease_1738972..1739122_POSITIVE 254 104 61 65 19 tfor:TF1342|3end_possible_lipoprotein_1424620..1424770_POSITIVE 253 49 5 138 102 tfor:TF1246|3end_protease,_with_chaperone_function_1330429..1330579_POSITIVE 251 134 95 38 1 tfor:TF1519|3end_hypothetical_protein_1628022..1628172_POSITIVE 250 120 80 33 1 tfor:TF1259|3end_hypothetical_protein_1345204..1345354_POSITIVE 250 50 5 147 107 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE