Origin IGS: tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaacagccacacgttgcgatgttaccaaaaagagcatttatgttacaccgtcccactgaatgcaacaaaaagaaattccctggggatttttcctttgttcatcgctataataatttacgataaaaacaaaggtaacgagaagggctgaattccatacaaggattcagccctcatttttgtggttcaaaatacttttaccgcaataaatca Mask Tandem Repeat Region ================================================ tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Find is-nt database================================================ Query_seq: TF2385:TF2386|TF2385:TF2386:conserved hypothetical protein; possible MazG family protein:beta-galactosidase:->->:2551605..2551815 211 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2385:TF2386|TF2385:TF2386:conserved hypothetical protein; possible MazG family protein:beta-galactosidase:->->:2551605..2551815 211 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2385:TF2386|TF2385:TF2386:conserved hypothetical protein; possible MazG family protein:beta-galactosidase:->->:2551605..2551815 211 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 48 End: 170 ............................................... M L V W N S A L L V T F V F I V N Y Y S D E Q R K N P Q G I S F C C I Q W D G V T ......................................... Protein_Len: 34 Strand: - Start: 91 End: 192 .......................................................................................... R L N N Y R H V F S F D G P F K K K N C E T P R H L M F A R K P L M ................... Protein_Len: 44 Strand: - Start: 66 End: 197 ................................................................. G K E N G K N K D Y I I I A I F L P F I G L S N R K T A N L P V T Y C L H E K Q Y C R M .............. tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 76 HP_score: -5.0 Tail_Score: -4.98255 Start: 38 End: 72 Full_Region: aaacaaaggtaacga gaagggctgaattc catacaa ggattcagccctca tttttgtggttcaaa .....................................gaagggctgaattccatacaaggattcagccctca........................................................................................................................................... TransTerm Strand: - Conf: 72 HP_score: -8.5 Tail_Score: -3.5823 Start: 39 End: 71 Full_Region: aacaaaggtaacgag aagggctgaattc catacaa ggattcagccctc atttttgtggttcaa ......................................aagggctgaattccatacaaggattcagccctc............................................................................................................................................ TransTerm Strand: + Conf: 80 HP_score: -9.7 Tail_Score: -3.55181 Start: 41 End: 69 Full_Region: GAACCACAAAAATGA GGGCTGAATCC TTGTAT GGAATTCAGCCC TTCTCGTTACCTTTG ........................................GGGCTGAATCCTTGTATGGAATTCAGCCC.............................................................................................................................................. TransTerm Strand: - Conf: 82 HP_score: -11.1 Tail_Score: -3.35709 Start: 41 End: 69 Full_Region: caaaggtaacgagaa gggctgaattc catacaa ggattcagccc tcatttttgtggttc ........................................gggctgaattccatacaaggattcagccc.............................................................................................................................................. TransTerm Strand: - Conf: 73 HP_score: -3.2 Tail_Score: -5.09521 Start: 125 End: 135 Full_Region: tgcaacaaaaagaaa ttcc ctg ggga tttttcctttgttca ............................................................................................................................ttccctgggga............................................................................ Find igs database================================================ Query_seq: TF2385:TF2386|TF2385:TF2386:conserved hypothetical protein; possible MazG family protein:beta-galactosidase:->->:2551605..2551815 211 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Intra-Species Hit: Count: 1 Min: 1 Max: 211 Len: 211 Subject: tfor_TF2385_TF2386|conserved hypothetical protein; possible MazG family protein:beta-galactosidase|POSITIVE:POSITIVE|[2551605, 2551815]|211 HSP 1 e-value: 1.0E-116 bit: 418.0 Len: 211 Query Start:1 Query End:211 Subject Strand: POSITIVE Subject Start: 1 Subject End: 211 tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga tgatttattgcggtaaaagtattttgaaccacaaaaatgagggctgaatccttgtatggaattcagcccttctcgttacctttgtttttatcgtaaattattatagcgatgaacaaaggaaaaatccccagggaatttctttttgttgcattcagtgggacggtgtaacataaatgctctttttggtaacatcgcaacgtgtggctgttga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUUAUUGCGGUAAAAGUAUUUUGAACCACAAAAAUGAGGGCUGAAUCCUUGUAUGGAAUUCAGCCCUUCUCGUUACCUUUGUUUUUAUCGUAAAUUAUUAUAGCGAUGAACAAAGGAAAAAUCCCCAGGGAAUUUCUUUUUGUUGCAUUCAGUGGGACGGUGUAACAUAAAUGCUCUUUUUGGUAACAUCGCAACGUGUGGCUGUUGA ((((...((((((((((((..(((((.....)))))..(((((((((((((......)).))))))))))).............))))))))))))...))))..((((((.((((((..(((.(((....))).)))..)))))).(((((..(((..((...))..))).)))))............))))))................ (-56.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAACAGCCACACGUUGCGAUGUUACCAAAAAGAGCAUUUAUGUUACACCGUCCCACUGAAUGCAACAAAAAGAAAUUCCCUGGGGAUUUUUCCUUUGUUCAUCGCUAUAAUAAUUUACGAUAAAAACAAAGGUAACGAGAAGGGCUGAAUUCCAUACAAGGAUUCAGCCCUCAUUUUUGUGGUUCAAAAUACUUUUACCGCAAUAAAUCA .((((........))))...(((((((.......(((((((((..((...))..)).))))))).(((((..((((.(((....))).))))..)))))..((((.((........)))))).........))))))).((.(((((((((.((......)))))))))))))....(((((((.............)))))))....... (-53.12) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 80 37 71 22 tfor:TF0847|5end_hypothetical_protein_897633..897863_POSITIVE 336 76 37 58 8 tfor:TF0970|5end_2-keto-3-deoxygluconate_kinase_1020396..1020626_POSITIVE 332 79 32 228 175 tfor:TF1969|5end_transcriptional_regulator,_AsnC_family_2116036..2116266_POSITIVE 324 210 161 188 138 tfor:TF2819|5end_N-acetyl-gamma-glutamylphosphate_reductase_3008905..3009135_POSITIVE 324 75 41 192 158 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 320 76 33 211 175 tfor:TF2664|5end_hypothetical_protein_2841399..2841629_POSITIVE 315 210 172 38 7 tfor:TF2043|5end_fructokinase_2202267..2202497_POSITIVE 313 210 161 83 31 tfor:TF0827|5end_conserved_hypothetical_protein_886240..886470_POSITIVE 310 76 40 52 13 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 302 75 37 115 80 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 302 75 37 115 80 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 298 75 41 206 172 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 298 75 41 206 172 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 293 88 42 82 23 tfor:TF2424|5end_hypothetical_protein_2603032..2603262_POSITIVE 293 207 175 40 1 tfor:TF2264|5end_transfer_region-related_protein,_TraO_2432890..2433120_POSITIVE 291 90 42 90 37 tfor:TF2118|5end_conserved_hypothetical_protein_2289961..2290191_POSITIVE 289 90 43 148 97 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 289 167 123 66 15 tfor:TF2603|5end_transcriptional_regulator,_AraC_family_2776578..2776808_POSITIVE 286 76 37 228 194 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 285 209 161 110 62 tfor:TF2134|5end_conserved_hypothetical_protein_2308689..2308919_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 77 31 55 6 tfor:TF2385|3end_conserved_hypothetical_protein;_possible_MazG_family_protein_2551544..2551694_POSITIVE 342 78 36 139 96 tfor:TF0465|3end_cobalamin_biosynthesis_protein_483913..484063_POSITIVE 342 80 37 108 59 tfor:TF0350|3end_hypothetical_protein_371101..371251_POSITIVE 332 79 32 71 18 tfor:TF1968|3end_conserved_hypothetical_protein_2115959..2116109_POSITIVE 320 76 33 55 19 tfor:TF2663|3end_hypothetical_protein_2841323..2841473_POSITIVE 315 210 172 47 16 tfor:TF2042|3end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2202356..2202506_POSITIVE 313 210 161 53 1 tfor:TF0826|3end_primosomal_protein_N'_886290..886440_POSITIVE 302 75 37 51 16 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 291 87 41 147 97 tfor:TF1782|3end_conserved_hypothetical_protein_1923333..1923483_POSITIVE 289 75 37 79 44 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 289 75 37 126 91 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 285 209 161 110 62 tfor:TF2132|3end_TIM-barrel_protein,_NifR3_family_2308769..2308919_POSITIVE 283 209 161 59 9 tfor:TF2224|3end_CTn_excision_protein_2394968..2395118_POSITIVE 282 199 151 142 87 tfor:TF1931|3end_ATPase/GTPase_2078622..2078772_POSITIVE 280 207 161 149 102 tfor:TF2818|3end_acetylornithine_aminotransferase_3008949..3009099_POSITIVE 279 210 161 71 12 tfor:TF2846|3end_cobyric_acid_synthase_3036469..3036619_POSITIVE 278 68 42 75 44 tfor:TF2251|3end_conjugative_transposon_protein,_TraE_2424687..2424837_POSITIVE 276 80 39 67 25 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 274 75 39 60 14 tfor:TF2490|3end_4-hydroxyphenylacetate-3-hydroxylase/4-hydroxybut_yryl_dehydratase_2666755..2666905_POSITIVE 273 210 164 136 101 tfor:TF0214|3end_DNA_recombination_and_repair_protein,_RecN_241571..241721_POSITIVE