Origin IGS: aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtgttttctctatacaatattttttaggttgcaaaactacgttgttttcaataattatgcaactccgccagactgccttcaggcattttagcatgaaaactgttatttttgcatt Mask Tandem Repeat Region ================================================ aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Find is-nt database================================================ Query_seq: TF2383:TF2384|TF2383:TF2384:hypothetical protein:thiol:disulfide interchange protein:->->:2548626..2548740 115 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2383:TF2384|TF2383:TF2384:hypothetical protein:thiol:disulfide interchange protein:->->:2548626..2548740 115 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2383:TF2384|TF2383:TF2384:hypothetical protein:thiol:disulfide interchange protein:->->:2548626..2548740 115 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 10 End: 114 ......... M T V F M L K C L K A V W R S C I I I E N N V V L Q P K K Y C I E K T . Protein_Len: 34 Strand: - Start: 3 End: 104 .. A F I V T K M S F H R F A T Q R L Q M I I S F L T T K C G L F Y Q M ........... aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 43 HP_score: -2.6 Tail_Score: -3.08046 Start: 32 End: 49 Full_Region: taattatgcaactcc gccaga ctgcct tcaggc attttagcatgaaaa ...............................gccagactgccttcaggc.................................................................. Find igs database================================================ Query_seq: TF2383:TF2384|TF2383:TF2384:hypothetical protein:thiol:disulfide interchange protein:->->:2548626..2548740 115 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Intra-Species Hit: Count: 1 Min: 1 Max: 115 Len: 115 Subject: tfor_TF2383_TF2384|hypothetical protein:thiol:disulfide interchange protein|POSITIVE:POSITIVE|[2548626,2548740]|115 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 115 Query Start:1 Query End:115 Subject Strand: POSITIVE Subject Start: 1 Subject End: 115 aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac aatgcaaaaataacagttttcatgctaaaatgcctgaaggcagtctggcggagttgcataattattgaaaacaacgtagttttgcaacctaaaaaatattgtatagagaaaacac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGCAAAAAUAACAGUUUUCAUGCUAAAAUGCCUGAAGGCAGUCUGGCGGAGUUGCAUAAUUAUUGAAAACAACGUAGUUUUGCAACCUAAAAAAUAUUGUAUAGAGAAAACAC ...............((((((..((((...((((....))))...))))...((((((.((((((((....))..)))))).))))))...................)))))).. (-26.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGUUUUCUCUAUACAAUAUUUUUUAGGUUGCAAAACUACGUUGUUUUCAAUAAUUAUGCAACUCCGCCAGACUGCCUUCAGGCAUUUUAGCAUGAAAACUGUUAUUUUUGCAUU ..((((((..................(((((((.......((((....)))).....)))))))..((.(((.((((....)))).))).))..))))))............... (-22.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 57 18 213 179 tfor:TF0995|5end_conserved_hypothetical_protein_1049695..1049925_POSITIVE 332 59 22 93 53 tfor:TF0058|5end_conserved_hypothetical_protein_59833..60063_POSITIVE 331 57 21 61 22 tfor:TF2523|5end_NAD(P)H-flavin_oxidoreductase_2702114..2702344_POSITIVE 331 50 1 219 177 tfor:TF0511|5end_hypothetical_protein_536926..537156_POSITIVE 311 59 30 181 156 tfor:TF0554|5end_Bacteroides_aerotolerance_protein,_BatC_586303..586533_POSITIVE 304 57 11 227 164 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 301 59 30 223 196 tfor:TF2102|5end_hypothetical_protein_2274943..2275173_POSITIVE 299 79 32 222 177 tfor:TF1889|5end_hypothetical_protein_2041479..2041709_POSITIVE 298 57 13 141 94 tfor:TF2670|5end_hypothetical_protein_2842779..2843009_POSITIVE 298 57 13 155 108 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 297 57 15 128 91 tfor:TF0746|5end_possible_DNA_polymerase_III_792261..792491_POSITIVE 296 56 24 190 149 tfor:TF2358|5end_possible_small-conductance_mechanosensitive_channel_2523829..2524059_POSITIVE 296 70 22 91 29 tfor:TF0782|5end_ThiJ/PfpI_family_protein_835976..836206_POSITIVE 288 62 23 95 52 tfor:TF0187|5end_ferredoxin_202151..202381_POSITIVE 287 57 16 85 51 tfor:TF0051|5end_hypothetical_protein_57327..57557_POSITIVE 285 57 14 215 168 tfor:TF1460|5end_6-phosphogluconate_dehydrogenase_1549262..1549492_POSITIVE 283 57 17 197 159 tfor:TF1937|5end_conserved_hypothetical_protein_2084476..2084706_POSITIVE 282 57 25 189 161 tfor:TF2037|5end_conserved_hypothetical_protein;_possible_anaerobic_ribonucleoside-triphosphate_reductase_2196754..2196984_POSITIVE 281 56 14 76 31 tfor:TF1473|5end_hypothetical_protein_1566341..1566571_POSITIVE 279 61 21 64 16 tfor:TF2661|5end_hypothetical_protein_2833509..2833739_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 297 57 15 95 58 tfor:TF0744|3end_DNA_polymerase_III_subunit,_gamma/tau_792308..792458_POSITIVE 290 58 15 141 90 tfor:TF1943|3end_phosphoenolpyruvate_carboxykinase_2092465..2092615_POSITIVE 279 52 12 149 102 tfor:TF2126|3end_S-adenosylhomocysteine_hydrolase_2303996..2304146_POSITIVE 273 57 14 80 20 tfor:TF2774|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_2959719..2959869_POSITIVE 273 58 14 77 34 tfor:TF2749|3end_possible_hemolysin_2934518..2934668_POSITIVE 273 57 24 46 11 tfor:TF0093|3end_outer_membrane_protein,_TonB_dependent_receptor_103618..103768_POSITIVE 272 51 14 119 80 tfor:TF2347|3end_outer_membrane_protein,_TonB_dependent_receptor_2511092..2511242_POSITIVE 267 80 31 122 66 tfor:TF0419|3end_conserved_hypothetical_protein_435794..435944_POSITIVE 266 57 16 148 109 tfor:TF1397|3end_glucosyltransferase_1471534..1471684_POSITIVE 265 79 32 109 58 tfor:TF2734|3end_conserved_hypothetical_protein_2916738..2916888_POSITIVE 264 58 17 146 95 tfor:TF2942|3end_50S_ribosomal_protein_L10_3143150..3143300_POSITIVE 263 110 61 104 50 tfor:TF0872|3end_conserved_hypothetical_protein_924045..924195_POSITIVE 262 58 35 100 66 tfor:TF0528|3end_two-component_system_sensor_histidine_kinase_554779..554929_POSITIVE 256 56 18 50 9 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 256 64 24 90 49 tfor:TF1362|3end_hypothetical_protein_1444059..1444209_POSITIVE 256 57 13 148 94 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 256 57 13 108 54 tfor:TF0468|3end_hypothetical_protein_487188..487338_POSITIVE 255 60 20 62 7 tfor:TF1834|3end_3-isopropylmalate_dehydrogenase_1984311..1984461_POSITIVE 255 57 16 70 16 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 254 76 33 143 100 tfor:TF2953|3end_possible_TonB-dependent_outer_membrane_receptor_3161501..3161651_POSITIVE