Origin IGS: taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacatcctccacagcttacataaacccctgattccgcaatagaagaaaagggttgaggggttgagttgttgaatgtttagctgatcgccgtaaaagccctgaaagggcgtgttaattcagcccaatgcggagcgttggggaaaaagcaatatccccgttgggagggctgtaagcctgacgtgaatattgattta Mask Tandem Repeat Region ================================================ taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Find is-nt database================================================ Query_seq: TF2350:TF2351|TF2350:TF2351:hypothetical protein:acetate kinase:->->:2515425..2515619 195 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2350:TF2351|TF2350:TF2351:hypothetical protein:acetate kinase:->->:2515425..2515619 195 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2350:TF2351|TF2350:TF2351:hypothetical protein:acetate kinase:->->:2515425..2515619 195 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 8 End: 115 ....... M F T S G L Q P S Q R G Y C F F P N A P H W A E L T R P F R A F T A I S ................................................................................ Protein_Len: 39 Strand: + Start: 79 End: 195 .............................................................................. M N T P F Q G F Y G D Q L N I Q Q L N P S T L F F Y C G I R G L C K L W R M L Protein_Len: 38 Strand: - Start: 1 End: 114 L D I N V D P K C G E W R P Y Q K K G L A G C Q A S N V R G K L A K V A I M ................................................................................. taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 78 HP_score: -9.1 Tail_Score: -4.08767 Start: 87 End: 99 Full_Region: GGGCTGAATTAACAC GCCCT TTC AGGGC TTTTACGGCGATCAG ......................................................................................GCCCTTTCAGGGC................................................................................................ Find igs database================================================ Query_seq: TF2350:TF2351|TF2350:TF2351:hypothetical protein:acetate kinase:->->:2515425..2515619 195 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Intra-Species Hit: Count: 1 Min: 1 Max: 195 Len: 195 Subject: tfor_TF2350_TF2351|hypothetical protein:acetate kinase|POSITIVE:POSITIVE|[2515425,2515619]|195 HSP 1 e-value: 1.0E-106 bit: 387.0 Len: 195 Query Start:1 Query End:195 Subject Strand: POSITIVE Subject Start: 1 Subject End: 195 taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta taaatcaatattcacgtcaggcttacagccctcccaacggggatattgctttttccccaacgctccgcattgggctgaattaacacgccctttcagggcttttacggcgatcagctaaacattcaacaactcaacccctcaacccttttcttctattgcggaatcaggggtttatgtaagctgtggaggatgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCAAUAUUCACGUCAGGCUUACAGCCCUCCCAACGGGGAUAUUGCUUUUUCCCCAACGCUCCGCAUUGGGCUGAAUUAACACGCCCUUUCAGGGCUUUUACGGCGAUCAGCUAAACAUUCAACAACUCAACCCCUCAACCCUUUUCUUCUAUUGCGGAAUCAGGGGUUUAUGUAAGCUGUGGAGGAUGUUA ......(((((((...(((((((((((....((((...(((((..........))))).....((((((..(((.(((........((((.....))))......(((.....)))......................))).)))...........))))))....))))....)))))))).))).))))))). (-56.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAUCCUCCACAGCUUACAUAAACCCCUGAUUCCGCAAUAGAAGAAAAGGGUUGAGGGGUUGAGUUGUUGAAUGUUUAGCUGAUCGCCGUAAAAGCCCUGAAAGGGCGUGUUAAUUCAGCCCAAUGCGGAGCGUUGGGGAAAAAGCAAUAUCCCCGUUGGGAGGGCUGUAAGCCUGACGUGAAUAUUGAUUUA .(((((..((.((((((((.....((((((.(..((..............))..).)))))))))))))).)))))))..(((((......(((..((((.....))))...)))..))))).(((((....((((..((.....(((....((((....))))..))).....))..))))...)))))..... (-61.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 983 70 21 86 37 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 983 70 21 221 172 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 961 68 21 79 26 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 958 80 31 179 130 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 958 80 31 58 9 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 877 100 51 190 139 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 871 100 51 189 140 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 865 109 61 185 137 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 857 100 51 193 144 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 788 109 61 225 177 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 786 100 51 219 166 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 782 100 51 78 30 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 756 100 52 123 65 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 756 100 52 116 58 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 725 100 51 64 4 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 637 108 61 131 79 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 632 80 33 230 178 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 605 70 21 191 143 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 601 90 41 102 43 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 584 68 21 160 111 tfor:TF1406|5end_transposase_1481758..1481988_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 857 100 51 108 59 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 788 109 61 79 31 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 782 100 51 61 13 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 746 100 51 82 24 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 688 46 6 151 111 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 637 108 61 61 9 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 627 90 47 136 92 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 584 68 21 141 92 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 566 68 21 140 86 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 555 76 36 40 1 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 488 43 1 135 84 tfor:TF0369|3end_hypothetical_protein_386186..386336_POSITIVE 487 165 121 65 10 tfor:TF2744|3end_methionyl-tRNA_formyltransferase_2927378..2927528_POSITIVE 484 58 16 127 79 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 445 59 17 132 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 408 149 105 114 70 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 383 109 61 81 24 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 367 100 53 150 98 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 363 75 40 40 1 tfor:TF2442|3end_heat_shock_protein_60_2620271..2620421_POSITIVE 363 68 28 143 103 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 357 90 41 56 4 tfor:TF1796|3end_ABC_transporter,_ATP-binding_protein_1937216..1937366_POSITIVE