Origin IGS: tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgatagtcatacttttccgggcacacgcttctcataagcttatcgggacaaaaaaagtcattttttaagagggtgtatcaaaacgcgatgtttaggtacatcctctttagtttaggttcatccgtgtcactccttgtggcta Mask Tandem Repeat Region ================================================ tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Find is-nt database================================================ Query_seq: TF2319:TF2320|TF2319:TF2320:acyl carrier protein:hypothetical protein:->->:2476649..2476793 145 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2319:TF2320|TF2319:TF2320:acyl carrier protein:hypothetical protein:->->:2476649..2476793 145 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2319:TF2320|TF2319:TF2320:acyl carrier protein:hypothetical protein:->->:2476649..2476793 145 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 2 End: 109 . A V L L S V S S G L S F L I Y R F M A N Q Y V R K F F S K K Q G S L S M .................................... tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 91 HP_score: -11.7 Tail_Score: -4.10672 Start: 38 End: 74 Full_Region: aaagtcattttttaa gagggtgtatcaaaacg cga tgtttaggtacatcctc tttagtttaggttca .....................................gagggtgtatcaaaacgcgatgtttaggtacatcctc....................................................................... Find igs database================================================ Query_seq: TF2319:TF2320|TF2319:TF2320:acyl carrier protein:hypothetical protein:->->:2476649..2476793 145 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Intra-Species Hit: Count: 1 Min: 1 Max: 145 Len: 145 Subject: tfor_TF2319_TF2320|acyl carrier protein:hypothetical protein|POSITIVE:POSITIVE|[2476649,2476793]|145 HSP 1 e-value: 2.0E-64 bit: 246.0 Len: 145 Query Start:1 Query End:145 Subject Strand: POSITIVE Subject Start: 1 Subject End: 145 tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgacnnnnnnngtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta tagccacaaggagtgacacggatgaacctaaactaaagaggatgtacctaaacatcgcgttttgatacaccctcttaaaaaatgactttttttgtcccgataagcttatgagaagcgtgtgcccggaaaagtatgactatcgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCACAAGGAGUGACACGGAUGAACCUAAACUAAAGAGGAUGUACCUAAACAUCGCGUUUUGAUACACCCUCUUAAAAAAUGACUUUUUUUGUCCCGAUAAGCUUAUGAGAAGCGUGUGCCCGGAAAAGUAUGACUAUCGUUA ..(.(((.....))).)...(((((..........((((((.((((.(.((((.....)))).).)))).))))))........(((((((..(.(.((....((((.....)))).)).).)..))))))).......))))). (-27.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAUAGUCAUACUUUUCCGGGCACACGCUUCUCAUAAGCUUAUCGGGACAAAAAAAGUCAUUUUUUAAGAGGGUGUAUCAAAACGCGAUGUUUAGGUACAUCCUCUUUAGUUUAGGUUCAUCCGUGUCACUCCUUGUGGCUA ......((((((((........(((((..((((.....))))......((((.((((((....))))))(((((((((((((.((((.....)))).)))))))))))))........))))....)))))......)))))))) (-39.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 579 76 33 163 114 tfor:TF0187|5end_ferredoxin_202151..202381_POSITIVE 478 76 33 199 155 tfor:TF0662|5end_hypothetical_protein_708316..708546_POSITIVE 453 76 35 76 35 tfor:TF1046|5end_hypothetical_protein_1108178..1108408_POSITIVE 451 76 36 213 173 tfor:TF1505|5end_conserved_hypothetical_protein_1610773..1611003_POSITIVE 412 76 34 149 107 tfor:TF2947|5end_isochorismate_synthase_3153633..3153863_POSITIVE 401 75 35 225 185 tfor:TF1999|5end_ArgK_protein_with_ATPase_and_kinase_domains_2152769..2152999_POSITIVE 400 76 35 203 161 tfor:TF2593|5end_amino_acid_transport_protein_2762882..2763112_POSITIVE 385 76 32 227 180 tfor:TF0184|5end_dihydroneopterin_aldolase_195629..195859_POSITIVE 332 76 36 158 122 tfor:TF2357|5end_collagenase_2522561..2522791_POSITIVE 329 139 98 222 181 tfor:TF0598|5end_hypothetical_protein_629190..629420_POSITIVE 327 76 38 192 155 tfor:TF3007|5end_conserved_hypothetical_protein_3227256..3227486_POSITIVE 321 125 83 180 142 tfor:TF2348|5end_outer_membrane_protein,_possibly_involved_in_nutrient_binding_2511088..2511318_POSITIVE 307 126 89 191 148 tfor:TF2368|5end_uridine_phosphorylase_2533666..2533896_POSITIVE 300 76 37 165 122 tfor:TF0792|5end_pyruvate_ferredoxin;_flavodoxin_oxidoreductase_846454..846684_POSITIVE 295 127 82 145 109 tfor:TF1317|5end_hypothetical_protein_1391378..1391608_POSITIVE 294 127 97 81 43 tfor:TF2879|5end_tRNA_(guanine-N-1)-methyltransferase_3068254..3068484_POSITIVE 291 138 94 198 141 tfor:TF2306|5end_hypothetical_protein_2466674..2466904_POSITIVE 290 139 93 75 15 tfor:TF2678|5end_GTP_cyclohydrolase_II_/3,4-dihydroxy-2-butanone-4-phoshate_synthase_2852036..2852266_POSITIVE 290 136 93 203 152 tfor:TF1689|5end_conserved_hypothetical_protein_1805068..1805298_POSITIVE 289 46 5 225 172 tfor:TF3000|5end_hypothetical_protein_3216412..3216642_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 509 74 33 49 7 tfor:TF2765|3end_subtilisin-like_serine_protease_2951460..2951610_POSITIVE 478 76 33 60 16 tfor:TF0661|3end_hypothetical_protein_708257..708407_POSITIVE 453 76 35 63 22 tfor:TF1045|3end_conserved_hypothetical_protein_1108245..1108395_POSITIVE 401 75 35 62 22 tfor:TF1997|3end_ROK_family_transcriptional_regulator_2152686..2152836_POSITIVE 400 76 35 48 6 tfor:TF2592|3end_conserved_hypothetical_protein_2762807..2762957_POSITIVE 377 76 35 46 5 tfor:TF0182|3end_DNA-binding_protein,_HU-related_195528..195678_POSITIVE 369 136 97 80 44 tfor:TF0686|3end_hypothetical_protein_732950..733100_POSITIVE 357 76 38 112 74 tfor:TF2413|3end_hypothetical_protein_2590680..2590830_POSITIVE 321 125 83 104 66 tfor:TF2347|3end_outer_membrane_protein,_TonB_dependent_receptor_2511092..2511242_POSITIVE 317 130 81 102 44 tfor:TF0658|3end_2-amino-3-ketobutyrate_coenzyme_A_ligase_705179..705329_POSITIVE 315 127 81 143 90 tfor:TF1968|3end_conserved_hypothetical_protein_2115959..2116109_POSITIVE 313 125 86 85 27 tfor:TF3053|3end_conserved_hypothetical_protein_3278889..3279039_POSITIVE 309 46 4 76 23 tfor:TF2657|3end_conserved_hypothetical_protein_2830873..2831023_POSITIVE 291 124 89 151 112 tfor:TF2367|3end_delta-1-pyrroline-5-carboxylate_dehydrogenase_2533710..2533860_POSITIVE 289 46 5 70 17 tfor:TF2590|3end_transposase_2757566..2757716_POSITIVE 284 110 63 134 92 tfor:TF0845|3end_hypothetical_protein_896693..896843_POSITIVE 283 126 91 53 3 tfor:TF2498|3end_electron_transfer_flavoprotein,_alpha_subunit_2673885..2674035_POSITIVE 283 135 91 62 5 tfor:TF1989|3end_outer_membrane_protein,_possible_TonB_dependent_receptor_2141394..2141544_POSITIVE 277 129 82 64 8 tfor:TF1932|3end_pigmentation_and_extracellular_proteinase_regulator_2079755..2079905_POSITIVE 274 136 97 43 1 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE