Origin IGS: aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacaatgtcttgtaagaaactaaacggagcatcttcgtcttaagtgacgattctttattgcataatatatcgatactcctaacaatggtaaaagaagaataaggcataccataaccaccttatacagtacactacgctttttttcataacgcagcaagtcataaattgccaaacagtcggctgcaataattaacgacaatacaatgaggttaaataattgtctttccattactgttctgttttaataattaattcggttgaagaacatgaagccaaagaattcaattactcttaagaatagcattcaatgcgcttaccccgcagccaaagcttgtccgaattggtatgctatttcaccggccacataaatgataatggacaggcagaataaaatcaccaatgccttatccgcattcgattctttgttaaaaatacaccgtttcatacgtttcattttttattgttaaacattcattgatgcgaaattaaaaagaagatttcaattatccaattccgattcgaaaaaaataattcgcacagatcatcaaaacgtattcagttatgccctttcataacgcgtccgtattggcacaatgtaatgcgtttcgattgtccgaaaaggcggcaagtggccggagaagtaaatcggtcactctccggtcaaaaacatcacatcctgtcttgccgagtaaaccctgaaatcatcggataagtgctgcactttcacgaaacaaaatcagagtgtaataagcatgcttaaaatcaacaagaccagcgaacatgccaacaatgcatattttatcttcttattcttgcatcgtatagccaatacaagaatgcatgaggcactagctataaataacatctttctcttttctcttctttttaattatacttgttttattttatttccgtaatcggctctatttttttatccataccgcaaaaccttt Mask Tandem Repeat Region ================================================ aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Find is-nt database================================================ Query_seq: TF2287:TF2288|TF2287:TF2288:hypothetical protein:hypothetical protein:->->:2441520..2442472 953 aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2287:TF2288|TF2287:TF2288:hypothetical protein:hypothetical protein:->->:2441520..2442472 953 aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2287:TF2288|TF2287:TF2288:hypothetical protein:hypothetical protein:->->:2441520..2442472 953 aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 89 End: 214 ........................................................................................ M L F I A S A S C I L V L A I R C K N K K I K Y A L L A C S L V L L I L S M L I T L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 157 End: 249 ............................................................................................................................................................ M C I V G M F A G L V D F K H A Y Y T L I L F R E S A A L I R ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 46 Strand: + Start: 500 End: 637 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K R M K R C I F N K E S N A D K A L V I L F C L S I I I Y V A G E I A Y Q F G Q A L A A G ............................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 768 End: 872 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Q P T V W Q F M T C C V M K K S V V Y C I R W L W Y A L F F F Y H C ................................................................................. Protein_Len: 60 Strand: + Start: 725 End: 931 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M E R Q L F N L I V L S L I I A A D C L A I Y D L L R Y E K K R S V L Y K V V M V C L I L L L P L L G V S I Y Y A I K N ...................... Protein_Len: 35 Strand: + Start: 847 End: 951 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M P Y S S F T I V R S I D I L C N K E S S L K T K M L R L V S Y K T L .. Protein_Len: 38 Strand: - Start: 589 End: 702 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ T A P S I A Y W N P C A K A A P Y A C Q I S N K L T I S N K P K M N K L R M ........................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 585 End: 674 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ K H P R H F L M G I R V L K P Q P T L A N F A I R L L L Q M ....................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 490 End: 585 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... C Y F I F R I F R H I K L L S D F A S L A N T I K N Q R D M I M ................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 33 Strand: - Start: 382 End: 480 ............................................................................................................................................................................................................................................................................................................................................................................................. S L A Y S F V N Q H D T R I I K K S D S N S L Q F R R K L K A D M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 258 End: 413 ................................................................................................................................................................................................................................................................. P K S P L V P H S T K S R L T V S K S R R G S A A K E S L R F A N C Q A L V S A N H F P M V S Y T K I M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 245 End: 469 .................................................................................................................................................................................................................................................... Q G S L S R N V E G A V Q R R K P C D F R M V N H W Y P R T I F P C L Q I R K S S R H S N N K E F R F Q I I S I K K K M .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 40 End: 258 ....................................... F L F I N N I A L A E H M R T N A I R H L F L F I F Y A N N A H E S T K N I K L M S I V S Q N Q K T F T C C K D S S K M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 3 End: 95 .. P K A T H I F F Y L R N R F Y F L V L I I L F F L F S F S T M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 708 End: 737 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTGTCTTTCCATTACTGTTCTGTTTTAAT........................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 861 End: 890 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTTACCATTGTTAGGAGTATCGATATATT............................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2287:TF2288|TF2287:TF2288:hypothetical protein:hypothetical protein:->->:2441520..2442472 953 aaaggttttgcggtatggataaaaaaatagagccgattacggaaataaaataaaacaagtataattaaaaagaagagaaaagagaaagatgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Intra-Species Hit: Count: 1 Min: 90 Max: 953 Len: 864 Subject: tfor_TF2287_TF2288|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2441520,2442472]|953 HSP 1 e-value: 0.0 bit: 1629.0 Len: 864 Query Start:90 Query End:953 Subject Strand: POSITIVE Subject Start: 90 Subject End: 953 .........................................................................................tgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattannnnnnncgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgnnnnnnngcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt .........................................................................................tgttatttatagctagtgcctcatgcattcttgtattggctatacgatgcaagaataagaagataaaatatgcattgttggcatgttcgctggtcttgttgattttaagcatgcttattacactctgattttgtttcgtgaaagtgcagcacttatccgatgatttcagggtttactcggcaagacaggatgtgatgtttttgaccggagagtgaccgatttacttctccggccacttgccgccttttcggacaatcgaaacgcattacattgtgccaatacggacgcgttatgaaagggcataactgaatacgttttgatgatctgtgcgaattatttttttcgaatcggaattggataattgaaatcttctttttaatttcgcatcaatgaatgtttaacaataaaaaatgaaacgtatgaaacggtgtatttttaacaaagaatcgaatgcggataaggcattggtgattttattctgcctgtccattatcatttatgtggccggtgaaatagcataccaattcggacaagctttggctgcggggtaagcgcattgaatgctattcttaagagtaattgaattctttggcttcatgttcttcaaccgaattaattattaaaacagaacagtaatggaaagacaattatttaacctcattgtattgtcgttaattattgcagccgactgtttggcaatttatgacttgctgcgttatgaaaaaaagcgtagtgtactgtataaggtggttatggtatgccttattcttcttttaccattgttaggagtatcgatatattatgcaataaagaatcgtcacttaagacgaagatgctccgtttagtttcttacaagacattgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUAUUUAUAGCUAGUGCCUCAUGCAUUCUUGUAUUGGCUAUACGAUGCAAGAAUAAGAAGAUAAAAUAUGCAUUGUUGGCAUGUUCGCUGGUCUUGUUGAUUUUAAGCAUGCUUAUUACACUCUGAUUUUGUUUCGUGAAAGUGCAGCACUUAUCCGAUGAUUUCAGGGUUUACUCGGCAAGACAGGAUGUGAUGUUUUUGACCGGAGAGUGACCGAUUUACUUCUCCGGCCACUUGCCGCCUUUUCGGACAAUCGAAACGCAUUACAUUGUGCCAAUACGGACGCGUUAUGAAAGGGCAUAACUGAAUACGUUUUGAUGAUCUGUGCGAAUUAUUUUUUUCGAAUCGGAAUUGGAUAAUUGAAAUCUUCUUUUUAAUUUCGCAUCAAUGAAUGUUUAACAAUAAAAAAUGAAACGUAUGAAACGGUGUAUUUUUAACAAAGAAUCGAAUGCGGAUAAGGCAUUGGUGAUUUUAUUCUGCCUGUCCAUUAUCAUUUAUGUGGCCGGUGAAAUAGCAUACCAAUUCGGACAAGCUUUGGCUGCGGGGUAAGCGCAUUGAAUGCUAUUCUUAAGAGUAAUUGAAUUCUUUGGCUUCAUGUUCUUCAACCGAAUUAAUUAUUAAAACAGAACAGUAAUGGAAAGACAAUUAUUUAACCUCAUUGUAUUGUCGUUAAUUAUUGCAGCCGACUGUUUGGCAAUUUAUGACUUGCUGCGUUAUGAAAAAAAGCGUAGUGUACUGUAUAAGGUGGUUAUGGUAUGCCUUAUUCUUCUUUUACCAUUGUUAGGAGUAUCGAUAUAUUAUGCAAUAAAGAAUCGUCACUUAAGACGAAGAUGCUCCGUUUAGUUUCUUACAAGACAUUGUU .........((((..(((((.(((((((((((((((((......))))))))))...............))))).))..)))))....))))((((((((((...((((....))))....(((((((..((...(((.(((((((...))))).))))).))..)))))))....))))))))))..(((((..(((....(((.(((((((((.........(((.((((((((....((((.((((.((..(((((((((.(((.(((((((((.(((((....)).))).....))))))).))))).)))))))))..)).(((((..(((((((((..((..((((((((((.....(....).....))))))))))..))......((.....))..)))))))))..))))))))).))))........................(((((.((((..((((....)))).)))))))))............)))))))).)))...((((((((.......(((((.(((((((((.((((((.((((.(((.((((.....((((((......))))))))))))))))).)))..)))....((((((.....((((.(((((...((..(((......)))..))..))))).))))...)))))).)))))))))..))))).((.((((((...(((((((((.........))))))))).....)))))).))....)))))))).)))))))))..............((((((((....((((....)))).....(((((......))))).)))))))).....)))....)))..)))))... (-231.96) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAAUGUCUUGUAAGAAACUAAACGGAGCAUCUUCGUCUUAAGUGACGAUUCUUUAUUGCAUAAUAUAUCGAUACUCCUAACAAUGGUAAAAGAAGAAUAAGGCAUACCAUAACCACCUUAUACAGUACACUACGCUUUUUUUCAUAACGCAGCAAGUCAUAAAUUGCCAAACAGUCGGCUGCAAUAAUUAACGACAAUACAAUGAGGUUAAAUAAUUGUCUUUCCAUUACUGUUCUGUUUUAAUAAUUAAUUCGGUUGAAGAACAUGAAGCCAAAGAAUUCAAUUACUCUUAAGAAUAGCAUUCAAUGCGCUUACCCCGCAGCCAAAGCUUGUCCGAAUUGGUAUGCUAUUUCACCGGCCACAUAAAUGAUAAUGGACAGGCAGAAUAAAAUCACCAAUGCCUUAUCCGCAUUCGAUUCUUUGUUAAAAAUACACCGUUUCAUACGUUUCAUUUUUUAUUGUUAAACAUUCAUUGAUGCGAAAUUAAAAAGAAGAUUUCAAUUAUCCAAUUCCGAUUCGAAAAAAAUAAUUCGCACAGAUCAUCAAAACGUAUUCAGUUAUGCCCUUUCAUAACGCGUCCGUAUUGGCACAAUGUAAUGCGUUUCGAUUGUCCGAAAAGGCGGCAAGUGGCCGGAGAAGUAAAUCGGUCACUCUCCGGUCAAAAACAUCACAUCCUGUCUUGCCGAGUAAACCCUGAAAUCAUCGGAUAAGUGCUGCACUUUCACGAAACAAAAUCAGAGUGUAAUAAGCAUGCUUAAAAUCAACAAGACCAGCGAACAUGCCAACAAUGCAUAUUUUAUCUUCUUAUUCUUGCAUCGUAUAGCCAAUACAAGAAUGCAUGAGGCACUAGCUAUAAAUAACA ...((((...((((.(((((.....((((.(((.(((((......))))).....((((....))))....))).))))((((((.((.....((((.(((((((((.........(((((((..............................((((........))))......((((..............))))......))))))).......((((((.(((((((.((((((...(((((..........)))))))))))....(((.((((..........))))..((((((((.....((((.......))))(((((..(......)..))))).))))))))....)))...........))))))).)))))).............)))))))))...).)))...)).))))))..........))))).))))...)))).(((((.((((....(((.((((((.((((((........))))))..................((((........)))).......))))))...((((((.((((((......))))))......((((((((..........((((((((..(((((((..((((((((((((((((((((.((.........))))))))))))...(((........)))))))))).......))).......))))))).((((((((((((..............)))))))....))))).....................)))).))))..((.(((((.....((......))....))))).))...))))))))..))))))..)))....)))).)))))..... (-182.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 413 250 202 148 99 tfor:TF1507|5end_outer_membrane_receptor,_TonB-dependent_1614536..1614766_POSITIVE 398 560 525 178 135 tfor:TF2899|5end_conserved_hypothetical_protein_3087495..3087725_POSITIVE 398 270 227 57 13 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 398 715 679 211 175 tfor:TF2484|5end_hypothetical_protein_2660005..2660235_POSITIVE 398 250 203 74 15 tfor:TF0301|5end_outer_membrane_protein,_TonB_dependent_receptor_325457..325687_POSITIVE 386 250 204 215 157 tfor:TF2775|5end_conserved_hypothetical_protein;_possible_mucoidy_inhibitor-related_protein_2959668..2959898_POSITIVE 382 240 199 76 40 tfor:TF1531|5end_potassium_uptake_protein_1636534..1636764_POSITIVE 378 260 211 180 132 tfor:TF2533|5end_adenylosuccinate_synthetase_2715666..2715896_POSITIVE 373 219 177 63 12 tfor:TF3122|5end_ABC_transporter,_ATP-binding_protein_3356047..3356277_POSITIVE 361 250 201 162 97 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 361 549 504 49 5 tfor:TF0419|5end_conserved_hypothetical_protein_435250..435480_POSITIVE 360 304 264 56 4 tfor:TF2729|5end_possible_outer_membrane_protein_2908216..2908446_POSITIVE 359 250 202 226 174 tfor:TF1467|5end_conserved_hypothetical_protein_1557011..1557241_POSITIVE 358 264 222 64 4 tfor:TF2573|5end_50S_ribosomal_protein_L15_2743310..2743540_POSITIVE 358 550 501 211 160 tfor:TF2020|5end_muconate_cycloisomerase_2176321..2176551_POSITIVE 358 239 194 184 139 tfor:TF1932|5end_pigmentation_and_extracellular_proteinase_regulator_2078542..2078772_POSITIVE 358 547 502 194 144 tfor:TF0348|5end_hypothetical_protein_369930..370160_POSITIVE 356 250 205 227 180 tfor:TF0754|5end_GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-redu_ctase_(GDP-L-fucose_synthetase)_801631..801861_POSITIVE 353 244 202 141 92 tfor:TF2674|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2848176..2848406_POSITIVE 353 568 528 199 149 tfor:TF1910|5end_nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase_2064001..2064231_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 398 715 679 148 112 tfor:TF2483|3end_hypothetical_protein_2660022..2660172_POSITIVE 398 250 203 78 19 tfor:TF0300|3end_hypothetical_protein_325541..325691_POSITIVE 382 240 199 76 40 tfor:TF1530|3end_1-deoxyxylulose-5-phosphate_synthase_1636614..1636764_POSITIVE 381 250 206 89 43 tfor:TF0242|3end_conserved_hypothetical_protein_274069..274219_POSITIVE 378 260 211 142 94 tfor:TF2532|3end_ferric-uptake_regulator_2715708..2715858_POSITIVE 373 219 177 60 9 tfor:TF3121|3end_nicotinate_phosphoribosyltransferase_3356124..3356274_POSITIVE 361 264 228 119 77 tfor:TF2893|3end_conserved_hypothetical_protein;_possible_phosphoesterase_3082039..3082189_POSITIVE 361 549 504 45 1 tfor:TF0418|3end_possible_methyl-accepting_chemotaxis_protein_435326..435476_POSITIVE 357 114 72 148 103 tfor:TF1215|3end_haloacid_dehalogenase-like_hydrolase_1303216..1303366_POSITIVE 351 549 504 58 9 tfor:TF0264|3end_ISPg3_(IS195)_transposase_293159..293309_POSITIVE 343 565 521 56 10 tfor:TF2442|3end_heat_shock_protein_60_2620271..2620421_POSITIVE 341 243 204 129 90 tfor:TF2472|3end_GAF_domain-containing_protein_2650468..2650618_POSITIVE 341 263 226 60 16 tfor:TF0412|3end_conserved_hypothetical_protein_430363..430513_POSITIVE 338 270 224 145 84 tfor:TF2660|3end_hypothetical_protein_2833540..2833690_POSITIVE 333 243 201 150 91 tfor:TF2433|3end_possible_UDP-2,3-diacylglucosamine_hydrolase_2614043..2614193_POSITIVE 332 250 205 106 63 tfor:TF2770|3end_conserved_hypothetical_protein_2956639..2956789_POSITIVE 328 549 501 146 93 tfor:TF1066|3end_hypothetical_protein_1136858..1137008_POSITIVE 327 549 504 59 19 tfor:TF1000|3end_sec-independent_protein_translocase_1054169..1054319_POSITIVE 327 288 241 71 8 tfor:TF0212|3end_DNA/pantothenate_metabolism_flavoprotein_239001..239151_POSITIVE 326 258 215 51 6 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE