Origin IGS: taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatatactacaatagatgcattgccgaaacgcaccttttaatacctcctcgtagcatgtggaaaactacaggattcttaactcgtattaatatgtatattattactgccgcataattgaacgcaaatttacgaaaaaaaagttccctgacaaaagcacaaagaacttttttaatctatacgtatttttatttggtattattaatttgttatttgatgatgagggctattcatataaatgtaggtta Mask Tandem Repeat Region ================================================ taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Find is-nt database================================================ Query_seq: TF2234:TF2235|TF2234:TF2235:acetyltransferase, GNAT family:tetracycline resistance protein related to CtnDOT tetQ:->->:2408139..2408385 247 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2234:TF2235|TF2234:TF2235:acetyltransferase, GNAT family:tetracycline resistance protein related to CtnDOT tetQ:->->:2408139..2408385 247 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2234:TF2235|TF2234:TF2235:acetyltransferase, GNAT family:tetracycline resistance protein related to CtnDOT tetQ:->->:2408139..2408385 247 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 47 End: 142 .............................................. M I P N K N T Y R L K K F F V L L S G N F F F V N L R S I M R Q ......................................................................................................... Protein_Len: 55 Strand: + Start: 73 End: 237 ........................................................................ M K K V L C A F V R E L F F R K F A F N Y A A V I I Y I L I R V K N P V V F H M L R G G I K R C V S A M H L L .......... Protein_Len: 37 Strand: - Start: 135 End: 245 ...................................................................................................................................... A A T I I Y M N I R T L F G T T K W M S R P P I L L H T E A I C R N Y Y M .. Protein_Len: 43 Strand: - Start: 3 End: 131 .. G V N I H I A R M M L Y C I L L V L Y F Y T Y I L F T R Q A K T L S S K K R L N A N M .................................................................................................................... Protein_Len: 45 Strand: - Start: 1 End: 135 L R C K Y S Y G E D D F L L N I I G F L F V Y L N F F N K T S K D P F K K K T F K R E I M ................................................................................................................ taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2234:TF2235|TF2234:TF2235:acetyltransferase, GNAT family:tetracycline resistance protein related to CtnDOT tetQ:->->:2408139..2408385 247 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Intra-Species Hit: Count: 1 Min: 1 Max: 247 Len: 247 Subject: tfor_TF2234_TF2235|acetyltransferase, GNAT family:tetracycline resistance protein related to CtnDOT tetQ|POSITIVE:POSITIVE|[2408139,2408385]|247 HSP 1 e-value: 1.0E-123 bit: 442.0 Len: 247 Query Start:1 Query End:247 Subject Strand: POSITIVE Subject Start: 1 Subject End: 247 taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacnnnnnnnncgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta taacctacatttatatgaatagccctcatcatcaaataacaaattaataataccaaataaaaatacgtatagattaaaaaagttctttgtgcttttgtcagggaacttttttttcgtaaatttgcgttcaattatgcggcagtaataatatacatattaatacgagttaagaatcctgtagttttccacatgctacgaggaggtattaaaaggtgcgtttcggcaatgcatctattgtagtatatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCUACAUUUAUAUGAAUAGCCCUCAUCAUCAAAUAACAAAUUAAUAAUACCAAAUAAAAAUACGUAUAGAUUAAAAAAGUUCUUUGUGCUUUUGUCAGGGAACUUUUUUUUCGUAAAUUUGCGUUCAAUUAUGCGGCAGUAAUAAUAUACAUAUUAAUACGAGUUAAGAAUCCUGUAGUUUUCCACAUGCUACGAGGAGGUAUUAAAAGGUGCGUUUCGGCAAUGCAUCUAUUGUAGUAUAUUA ....(((((......................................(((((((...........(((((((...((((((((((((((.((....))))))))))))))))..((((....)))).....)))))))...(((.(((((....))))).)))..........(((((((((.........))))).)))))))))))..(((((((((.....)))))))))..)))))....... (-54.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUACUACAAUAGAUGCAUUGCCGAAACGCACCUUUUAAUACCUCCUCGUAGCAUGUGGAAAACUACAGGAUUCUUAACUCGUAUUAAUAUGUAUAUUAUUACUGCCGCAUAAUUGAACGCAAAUUUACGAAAAAAAAGUUCCCUGACAAAAGCACAAAGAACUUUUUUAAUCUAUACGUAUUUUUAUUUGGUAUUAUUAAUUUGUUAUUUGAUGAUGAGGGCUAUUCAUAUAAAUGUAGGUUA .......(((((((((...((((..((((..(((.....(((((((((((.((((...........))))))))..........(((..((((((((((.......(((..((....))...)))..........((((((((((..((.........))..))))))))))....))))))))))..)))..)))))))......)))..))))..))))....)))).........))))).... (-44.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 421 230 181 86 36 tfor:TF0892|5end_conserved_hypothetical_protein_940836..941066_POSITIVE 357 240 194 159 105 tfor:TF0339|5end_hypothetical_protein_363470..363700_POSITIVE 311 203 163 62 21 tfor:TF1596|5end_hypothetical_protein_1711225..1711455_POSITIVE 310 226 181 152 99 tfor:TF1043|5end_conserved_hypothetical_protein;_possible_hydrolase_1105282..1105512_POSITIVE 309 222 181 229 196 tfor:TF2267|5end_lysozyme-related_protein_2434869..2435099_POSITIVE 309 235 192 79 34 tfor:TF2224|5end_CTn_excision_protein_2394592..2394822_POSITIVE 307 239 193 202 147 tfor:TF1800|5end_conserved_hypothetical_protein_1941214..1941444_POSITIVE 304 229 189 205 164 tfor:TF2673|5end_conserved_hypothetical_protein_2846987..2847217_POSITIVE 304 225 188 58 20 tfor:TF1305|5end_UDP-glucose_4-epimerase_1385623..1385853_POSITIVE 302 237 191 209 153 tfor:TF2899|5end_conserved_hypothetical_protein_3087495..3087725_POSITIVE 298 238 191 183 133 tfor:TF1888|5end_RNA_polymerase_ECF-type_sigma_factor_2040940..2041170_POSITIVE 296 140 92 70 16 tfor:TF1575|5end_DNA-binding_response_regulator_1685177..1685407_POSITIVE 296 229 189 136 89 tfor:TF0925|5end_hypothetical_protein_961736..961966_POSITIVE 295 230 184 98 38 tfor:TF2130|5end_conserved_hypothetical_protein;_possible_permease_2306486..2306716_POSITIVE 295 229 183 195 145 tfor:TF0595|5end_hypothetical_protein_627820..628050_POSITIVE 293 240 191 177 125 tfor:TF1253|5end_hypothetical_protein_1335982..1336212_POSITIVE 290 228 181 217 163 tfor:TF1763|5end_K+-dependent_Na+/Ca+_exchanger_related-protein_1900406..1900636_POSITIVE 288 233 192 150 111 tfor:TF2877|5end_regulatory_protein_3065601..3065831_POSITIVE 288 235 196 148 107 tfor:TF2739|5end_thioredoxin_2920126..2920356_POSITIVE 288 229 181 170 114 tfor:TF2189|5end_conserved_hypothetical_protein_2363455..2363685_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 310 226 181 85 32 tfor:TF1042|3end_possible_surface_antigen_with_leucine-rich_repeat_1105295..1105445_POSITIVE 306 238 199 88 40 tfor:TF3143|3end_conserved_hypothetical_protein;_possible_membrane_protein_3379227..3379377_POSITIVE 295 230 184 101 41 tfor:TF2129|3end_possible_3-oxoacyl-(acyl-carrier-protein)_synthase_2306569..2306719_POSITIVE 293 224 189 147 111 tfor:TF2672|3end_lipoic_acid_synthetase_2847014..2847164_POSITIVE 292 229 181 133 69 tfor:TF1187|3end_hypothetical_protein_1269872..1270022_POSITIVE 292 229 181 76 12 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 291 233 196 151 107 tfor:TF0895|3end_hypothetical_protein_945065..945215_POSITIVE 288 233 192 133 94 tfor:TF2876|3end_cytosine_deaminase_3065664..3065814_POSITIVE 284 224 182 56 9 tfor:TF1381|3end_hypothetical_protein_1456814..1456964_POSITIVE 280 224 188 35 4 tfor:TF0811|3end_conserved_hypothetical_protein;_posible_sugar_phosphate_isomerase_870724..870874_POSITIVE 280 225 190 80 35 tfor:TF0239|3end_conserved_hypothetical_protein_270323..270473_POSITIVE 279 228 194 151 111 tfor:TF0338|3end_alkaline_phosphatase_363556..363706_POSITIVE 271 223 193 37 13 tfor:TF0376|3end_conserved_hypothetical_protein_395024..395174_POSITIVE 266 224 183 134 94 tfor:TF2022|3end_conserved_hypothetical_protein_2180670..2180820_POSITIVE 262 229 181 67 14 tfor:TF1322|3end_conserved_hypothetical_protein;_possible_surface_protein_1402567..1402717_POSITIVE 260 239 191 51 8 tfor:TF3132|3end_conserved_hypothetical_protein_3368047..3368197_POSITIVE 260 239 191 109 53 tfor:TF2351|3end_acetate_kinase_2516807..2516957_POSITIVE 260 228 194 148 109 tfor:TF1260|3end_chaperone_protein_DnaJ_1346553..1346703_POSITIVE 260 230 184 50 6 tfor:TF1197|3end_transcriptional_regulator_1281414..1281564_POSITIVE 259 225 181 132 80 tfor:TF1988|3end_hypothetical_protein_2137481..2137631_POSITIVE