Origin IGS: taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaaatattccaactaaccttgtttatgttttgtcagccaccatttgcacaaccatatttgactcccaaagagagtgaattcgataagataacttgctgcatacccacaataaggggctaaatagaatacgaatgggaaatagataacagtaccgacaacagattgcgttatgtagttggttaaactcattttaccataaagctgtaatgtggaaaccgcttttttgacacgttccttatgacacaataatatgaacgagaatatttctacagaatcatcagtaaaacagaaaaaaatactaattttgtttttgatgatcatttcaaaaggaattagaaaaatatctaattctaacttatagaggaaaaagcaacaattaaatattaaagaataaaagatacgaaaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttttcgtatcttttattctttaatatttaattgttgctttttcctctataagttagaattagatatttttctaattccttttgaaatgatcatcaaaaacaaaattagtatttttttctgttttactgatgattctgtagaaatattctcgttcatattattgtgtcataaggaacgtgtcaaaaaagcggtttccacattacagctttatggtaaaatgagtttaaccaactacataacgcaatctgttgtcggtactgttatctatttcccattcgtattctatttagccccttattgtgggtatgcagcaagttatcttatcgaattcactctctttgggagtcaaatatggttgtgcaaatggtggctgacaaaacataaacaaggttagttggaatattttttggtataatgtttataaacataaatcaagaaagaataaataaatttaatagaggctgcccaaaaagaaagaaatccttacaatcactcttctacagatacttgcagaggggataagatttacttttagaccttttggatagcccctattaatattttatataaaattcataatttta Mask Tandem Repeat Region ================================================ taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaaatattccaactaaccttgtttatgttttgtcagccaccatttgcacaaccatatttgactcccaaagagagtgaattcgataagataacttgctgcatacccacaataaggggctaaatagaatacgaatgggaaatagataacagtaccgacaacagattgcgttatgtagttggttaaactcattttaccataaagctgtaatgtggaaaccgcttttttgacacgttccttatgacacaataatatgaacgagaatatttctacagaatcatcagtaaaacagaaaaaaatactaattttgtttttgatgatcatttcaaaaggaattagaaaaatatctaattctaacttatagaggaaaaagcaacaattaaatattaaagaataaaagatacgaaaaat Find is-nt database================================================ Query_seq: TF2231:TF2233|TF2231:TF2233:DNA methylase, site-specific DNA-methyltransferase (adenine-specific):conserved hypothetical protein:->->:2406309..2406894 586 taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaaatattccaactaaccttgtttatgttttgtcagccaccatttgcacaaccatatttgactcccaaagagagtgaattcgataagataacttgctgcatacccacaataaggggctaaatagaatacgaatgggaaatagataacagtaccgacaacagattgcgttatgtagttggttaaactcattttaccataaagctgtaatgtggaaaccgcttttttgacacgttccttatgacacaataatatgaacgagaatatttctacagaatcatcagtaaaacagaaaaaaatactaattttgtttttgatgatcatttcaaaaggaattagaaaaatatctaattctaacttatagaggaaaaagcaacaattaaatattaaagaataaaagatacgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2231:TF2233|TF2231:TF2233:DNA methylase, site-specific DNA-methyltransferase (adenine-specific):conserved hypothetical protein:->->:2406309..2406894 586 taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaaatattccaactaaccttgtttatgttttgtcagccaccatttgcacaaccatatttgactcccaaagagagtgaattcgataagataacttgctgcatacccacaataaggggctaaatagaatacgaatgggaaatagataacagtaccgacaacagattgcgttatgtagttggttaaactcattttaccataaagctgtaatgtggaaaccgcttttttgacacgttccttatgacacaataatatgaacgagaatatttctacagaatcatcagtaaaacagaaaaaaatactaattttgtttttgatgatcatttcaaaaggaattagaaaaatatctaattctaacttatagaggaaaaagcaacaattaaatattaaagaataaaagatacgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2231:TF2233|TF2231:TF2233:DNA methylase, site-specific DNA-methyltransferase (adenine-specific):conserved hypothetical protein:->->:2406309..2406894 586 taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaaatattccaactaaccttgtttatgttttgtcagccaccatttgcacaaccatatttgactcccaaagagagtgaattcgataagataacttgctgcatacccacaataaggggctaaatagaatacgaatgggaaatagataacagtaccgacaacagattgcgttatgtagttggttaaactcattttaccataaagctgtaatgtggaaaccgcttttttgacacgttccttatgacacaataatatgaacgagaatatttctacagaatcatcagtaaaacagaaaaaaatactaattttgtttttgatgatcatttcaaaaggaattagaaaaatatctaattctaacttatagaggaaaaagcaacaattaaatattaaagaataaaagatacgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 15 Min: 182 Max: 568 Len: 387 Subject: UniRef90_Q8A7H7 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 285 Query Start:185 Query End:469 Subject Strand: null Subject Start: 286 Subject End: 378 ........................................................................................................................................................................................ F C F T D D S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E ..................................................................................................................... ........................................................................................................................................................................................ F A F T - - I V L I A S F I L L Y Q K A K F Q K T V S N L R F Y G K M S L T N Y I T Q S I M G A I I Y F P F G F Y L A P Y C G Y T L S L I I G I I L F L L Q V Q F C K W W L S K H K Q G P L E ..................................................................................................................... Subject: UniRef90_Q64SV2 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis HSP 1 e-value: 2.0E-24 bit: 114.0 Len: 384 Query Start:185 Query End:568 Subject Strand: null Subject Start: 250 Subject End: 383 ........................................................................................................................................................................................ S L I F N C C F F - - L Y K L E L D I F L I P F E M I I K N K I S I F - - - - - - F C F T D D S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E .................. ........................................................................................................................................................................................ A L I W S A V L F G P L Y Q L K V Q L M D A G Q P D M I R Q T V G V V M D M W Q K F A F T - - I V L V A S F V L L Y Q T E K F R K L T A D L R F Y G K M S L T N Y I S Q S I A G A I I Y F P F A L Y L A P Y C G Y T V S L L I G F A F F L L Q V R F C K W W L K A H K Q G P L E .................. Subject: UniRef90_Q89ZB8 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron HSP 1 e-value: 5.0E-12 bit: 73.2 Len: 333 Query Start:182 Query End:514 Subject Strand: null Subject Start: 275 Subject End: 382 ..................................................................................................................................................................................... L I P F E M I I K N K I S I F F C F T D D S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ........................................................................ ..................................................................................................................................................................................... M V P Y K I A V P S Y A N - - F A F M V I L V S V F T - L L W F K K D T G Y S W Q S L L I P Y G R M S L T N Y I S Q S I M G V T I Y Y G F G L S M Y K Y A G A T G S L L I A L L I F T I Q L I F S R W W L A R H K Q G P L E F ........................................................................ Subject: UniRef90_Q8A424 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron HSP 1 e-value: 3.0E-10 bit: 67.4 Len: 342 Query Start:182 Query End:523 Subject Strand: null Subject Start: 273 Subject End: 382 ..................................................................................................................................................................................... D I F L I P F E M I I K N K I S I F F C F T D D S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ............................................................... ..................................................................................................................................................................................... E A L L V P S V L L C K S L S N I V F T - - - - G I M F A G I I L V Y Y L T T L K N V L H K F A P Y G R M S L T N Y L S Q S L I G G F L F Y N W G L G L Y L H T G I T A C I L I G V S V F F L Q Y F F C N W W L R S H R Q G P L E W ............................................................... Subject: UniRef90_A4AN58 Cluster: Hypothetical protein; n=1; Flavobacteriales bacterium HTCC2170|Rep: Hypothetical protein - Flavobacteriales bacterium HTCC2170 HSP 1 e-value: 4.0E-9 bit: 63.5 Len: 267 Query Start:185 Query End:451 Subject Strand: null Subject Start: 294 Subject End: 382 ........................................................................................................................................................................................ S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E ....................................................................................................................................... ........................................................................................................................................................................................ A V L V A S F I L L Y K T N I F R K I A G G L R Y C G R M S L T A Y V L Q S I I G G F V F F G Y G M G L G P K L L H T K S L A I G I V L F A G L F Y F C K W W I N T Y G Q G P L E ....................................................................................................................................... Subject: UniRef90_Q64R65 Cluster: Putative membrane-associated protein; n=2; Bacteroides fragilis|Rep: Putative membrane-associated protein - Bacteroides fragilis HSP 1 e-value: 3.0E-8 bit: 60.8 Len: 366 Query Start:185 Query End:550 Subject Strand: null Subject Start: 255 Subject End: 381 ........................................................................................................................................................................................ C F F - L Y K L E L D I F L I P F E M I I K N K I S I F F C F T D D S V E I F S F I L L C - - - - - - - - H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E .................................... ........................................................................................................................................................................................ C F F P L Y G L N N - - - M L P - E F I T R N A I R V P L Q L I I S S L S N L S F M V L L V S G L L I T F Y R I K D R R F L M R F T S Y G K M S L T N Y I G Q S V I G S L L F Y H W G F E L G R F L G I T Y S F F F G I L F V L L Q M A F C S W W L S H F K H G P F E .................................... Subject: UniRef90_Q2AJF7 Cluster: Hypothetical protein; n=3; Bacillus cereus group|Rep: Hypothetical protein - Bacillus weihenstephanensis KBAB4 HSP 1 e-value: 3.0E-8 bit: 60.8 Len: 255 Query Start:182 Query End:436 Subject Strand: null Subject Start: 285 Subject End: 369 ..................................................................................................................................................................................... S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ...................................................................................................................................................... ..................................................................................................................................................................................... T I I L L F H Y K K V A R L L T Y M A N M G K M S V S N Y L A Q S I I A T T I F Y A Y G F G L Y G K I G Y F F G I L L T I G I Y T I Q L F V S T Y W L Q K Y R M G P V E Y ...................................................................................................................................................... Subject: UniRef90_Q3EKV3 Cluster: Hypothetical membrane spanning protein; n=1; Bacillus thuringiensis serovar israelensis ATCC 35646|Rep: Hypothetical membrane spanning protein - Bacillus thuringiensis serovar israelensis ATCC 35646 HSP 1 e-value: 6.0E-8 bit: 59.7 Len: 255 Query Start:182 Query End:436 Subject Strand: null Subject Start: 176 Subject End: 260 ..................................................................................................................................................................................... S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ...................................................................................................................................................... ..................................................................................................................................................................................... T I I L L F H Y K K A S R L L T Y M A N M G K M S V S N Y L A Q S I I A T T I F Y A Y G F G L Y G K I G Y F F G I L L T I G I Y T I Q L F V S T Y W L Q K Y R M G P V E Y ...................................................................................................................................................... Subject: UniRef90_Q6HR76 Cluster: Hypothetical protein; n=1; Bacillus anthracis|Rep: Hypothetical protein - Bacillus anthracis HSP 1 e-value: 2.0E-7 bit: 58.2 Len: 255 Query Start:182 Query End:436 Subject Strand: null Subject Start: 160 Subject End: 244 ..................................................................................................................................................................................... S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ...................................................................................................................................................... ..................................................................................................................................................................................... T I I L L F H Y K K A T R L L T Y M A N M G K M S V S N Y L A Q S I I A T T I F Y A Y G F G L Y G K I G Y F F G I L L T I G I Y T I Q L V V S T Y W L Q K Y R M G P I E Y ...................................................................................................................................................... Subject: UniRef90_Q73AS3 Cluster: Membrane protein, putative; n=2; Bacillus cereus group|Rep: Membrane protein, putative - Bacillus cereus (strain ATCC 10987) HSP 1 e-value: 1.0E-6 bit: 55.5 Len: 261 Query Start:182 Query End:442 Subject Strand: null Subject Start: 282 Subject End: 367 ..................................................................................................................................................................................... I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F - - - Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ................................................................................................................................................ ..................................................................................................................................................................................... I F T L M R L L Q K E K W Q K I L R P F Q Y V G R M A L T N Y I S H T I V - T L L V F G I L F K N D Y L A P L W V G P L F C I G F Y T L - - - Q I F I S R W W L S R Y Q Y G P L E Y ................................................................................................................................................ Subject: UniRef90_Q1EZ96 Cluster: Hypothetical protein; n=1; Clostridium sp. OhILAs|Rep: Hypothetical protein - Clostridium sp. OhILAs HSP 1 e-value: 1.0E-6 bit: 55.1 Len: 291 Query Start:182 Query End:472 Subject Strand: null Subject Start: 271 Subject End: 369 ..................................................................................................................................................................................... F F C F T D D S V E I F - - - S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y .................................................................................................................. ..................................................................................................................................................................................... F V W F S G K T M A I F Y V T T I L L L L R K E K W R K R L K S F S Y V G K M A L T N Y I G Q T V V - S I F V F S M V F K N T A K I P L W V G L L Y C P I F Y I G Q I Q F S K W W L S T H K M G P L E W .................................................................................................................. Subject: UniRef90_A4ARZ9 Cluster: Putative transport protein; n=1; Flavobacteriales bacterium HTCC2170|Rep: Putative transport protein - Flavobacteriales bacterium HTCC2170 HSP 1 e-value: 4.0E-6 bit: 53.5 Len: 261 Query Start:182 Query End:442 Subject Strand: null Subject Start: 301 Subject End: 387 ..................................................................................................................................................................................... I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ................................................................................................................................................ ..................................................................................................................................................................................... I V L F I M L Y K K V K G E K M L R I F A P Y G R M A L S N Y F F Q S L L G T F I F F G W G M G Y L V E L R N G Y T F L I A L A V I V L Q M F I S K W W L R K F H Y G P L E W ................................................................................................................................................ Subject: UniRef90_Q5WCD3 Cluster: Hypothetical protein; n=1; Bacillus clausii KSM-K16|Rep: Hypothetical protein - Bacillus clausii (strain KSM-K16) HSP 1 e-value: 7.0E-6 bit: 52.8 Len: 258 Query Start:185 Query End:442 Subject Strand: null Subject Start: 308 Subject End: 393 ........................................................................................................................................................................................ I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E ................................................................................................................................................ ........................................................................................................................................................................................ V S A F V L L L Q K G T V A R L F M P I A S A G R M A L T N Y L L Q T I V A T T I F Y G Y G F G L F G S V S S S Q G L L I A I V I Y A T Q V I L S H L Y L K K W K Q G P L E ................................................................................................................................................ Subject: UniRef90_Q813S7 Cluster: Hypothetical Membrane Spanning Protein; n=1; Bacillus cereus ATCC 14579|Rep: Hypothetical Membrane Spanning Protein - Bacillus cereus (strain ATCC 14579 / DSM 31) HSP 1 e-value: 7.0E-6 bit: 52.8 Len: 363 Query Start:182 Query End:544 Subject Strand: null Subject Start: 241 Subject End: 367 ..................................................................................................................................................................................... F L Y K L E L D I F L I - - - - - - P F - E M I I K N K I S I F F C F T D D S V E I F S F I L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V - - F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y .......................................... ..................................................................................................................................................................................... F L F T L P M W F F M V R Y F L S K P F Y E P I Y M Q V F T M F S G K T L F I F Y I F T L M R L L Q K E K W Q T L L R P F Q Y V G R M A L T N Y I S H T I V T L L V F G L F L K N Y Y P A P L W V G P I F C I S F Y T L - - - Q I F I S R W W L S H Y Q Y G P L E Y .......................................... Subject: UniRef90_Q1XUY5 Cluster: Hypothetical protein; n=1; Flavobacterium johnsoniae UW101|Rep: Hypothetical protein - Flavobacterium johnsoniae UW101 HSP 1 e-value: 9.0E-6 bit: 52.4 Len: 246 Query Start:182 Query End:427 Subject Strand: null Subject Start: 290 Subject End: 371 ..................................................................................................................................................................................... L L C H K E R V K K A V S T L Q L Y G K M S L T N Y I T Q S V V G T V I Y F P F V F Y L A P Y C G Y A A S Y L I E F T L F G S Q I W L C K W W L T K H K Q G * L E Y ............................................................................................................................................................... ..................................................................................................................................................................................... L L Y S H K K L K T V F N Y F R A G G K M T L T N Y I A Q N I L G A F I F S G I G L G I A D T M P Y W F Y F S L A V V I F V L Q L F I S K W W L S K Y N Y G P I E W ............................................................................................................................................................... taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnataaaagatacgaaaaat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 26 End: 130 ......................... M N R G Y P K G L K V N L I P S A S I C R R V I V R I S F F L G S L Y ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 32 Strand: + Start: 157 End: 252 ............................................................................................................................................................ M F I N I I P K N I P T N L V Y V L S A T I C T T I F D S Q R E .............................................................................................................................................................................................................................................................................................................................................. Protein_Len: 38 Strand: + Start: 182 End: 295 ..................................................................................................................................................................................... M F Q L T L F M F C Q P P F A Q P Y L T P K E S E F D K I T C C I P T I R G ................................................................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: + Start: 429 End: 536 ............................................................................................................................................................................................................................................................................................................................................................................................................................................ M N E N I S T E S S V K Q K K I L I L F L M I I S K G I R K I S N S N L .................................................. Protein_Len: 46 Strand: - Start: 204 End: 341 ........................................................................................................................................................................................................... T K D A V M Q V V M N S E W L S H I R Y S L K S C V W L L P S F L I R I P F L Y C Y R C C M ..................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 194 End: 586 ................................................................................................................................................................................................. R E K H C L L I F S F I E V S D D T F C F F I S I K N K I I M E F P I L F I D L E L K Y L F F C C N F I L S Y F I R F M Protein_Len: 40 Strand: - Start: 66 End: 185 ................................................................. G R Q L Y R Y F L S Q L S K K R K P C G R N F K N I R E Q N I N I F M I G F F M ................................................................................................................................................................................................................................................................................................................................................................................................................. taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnataaaagatacgaaaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 401 End: 430 ................................................................................................................................................................................................................................................................................................................................................................................................................TTTGACACGTTCCTTATGACACAATAATAT............................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 526 End: 555 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTGTTGCTTTTTCCTCTATAAGTTAGAAT............................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnataaaagatacgaaaaat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 75 HP_score: -4.6 Tail_Score: -5.03145 Start: 387 End: 397 Full_Region: CCATAAAGCTGTAAT GTGG AAA CCGC TTTTTTGACACGTTC ..................................................................................................................................................................................................................................................................................................................................................................................................GTGGAAACCGC............................................................................................................................................................................................. TransTerm Strand: - Conf: 53 HP_score: -2.5 Tail_Score: -4.15833 Start: 507 End: 530 Full_Region: tttcctctataagtt agaattaga tatttt tctaattcc ttttgaaatgatcat ..........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agaattagatatttttctaattcc........................................................ TransTerm Strand: - Conf: 57 HP_score: -6.0 Tail_Score: -3.09018 Start: 508 End: 529 Full_Region: ttcctctataagtta gaattaga tatttt tctaattc cttttgaaatgatca ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gaattagatatttttctaattc......................................................... Find igs database================================================ Query_seq: TF2231:TF2233|TF2231:TF2233:DNA methylase, site-specific DNA-methyltransferase (adenine-specific):conserved hypothetical protein:->->:2406309..2406894 586 taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnataaaagatacgaaaaat Intra-Species Hit: Count: 1 Min: 1 Max: 181 Len: 181 Subject: tfor_TF2231_TF2233|DNA methylase, site-specific DNA-methyltransferase (adenine-specific):conserved hypothetical protein|POSITIVE:POSITIVE|[2406309,2406894]|586 HSP 1 e-value: 7.0E-98 bit: 359.0 Len: 181 Query Start:1 Query End:181 Subject Strand: POSITIVE Subject Start: 1 Subject End: 181 taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaa..................................................................................................................................................................................................................................................................................................................................................................................................................... taaaattatgaattttatataaaatattaataggggctatccaaaaggtctaaaagtaaatcttatcccctctgcaagtatctgtagaagagtgattgtaaggatttctttctttttgggcagcctctattaaatttatttattctttcttgatttatgtttataaacattataccaaaaa..................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUAUGAAUUUUAUAUAAAAUAUUAAUAGGGGCUAUCCAAAAGGUCUAAAAGUAAAUCUUAUCCCCUCUGCAAGUAUCUGUAGAAGAGUGAUUGUAAGGAUUUCUUUCUUUUUGGGCAGCCUCUAUUAAAUUUAUUUAUUCUUUCUUGAUUUAUGUUUAUAAACAUUAUACCAAAAA ..((((((.(((...((.(((((...((((((((((((..((((((((....((((.((((((((((((.((((((......)))))).).).)))....)))))))))))))))))))..)))))))))))).))))).))....))).))))))......................... (-44.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUGGUAUAAUGUUUAUAAACAUAAAUCAAGAAAGAAUAAAUAAAUUUAAUAGAGGCUGCCCAAAAAGAAAGAAAUCCUUACAAUCACUCUUCUACAGAUACUUGCAGAGGGGAUAAGAUUUACUUUUAGACCUUUUGGAUAGCCCCUAUUAAUAUUUUAUAUAAAAUUCAUAAUUUUA ((((((((...(((((....)))))..)))))))).((((..(((((.(((((((.(((((.((((((.((((((((((.(((......(((((((.(((....))).))))))).)))))))).))))).....)))))).))))).)))))))...))))).....))))......... (-39.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 127 89 158 113 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 313 127 89 89 45 tfor:TF2816|5end_hypothetical_protein_3006925..3007155_POSITIVE 304 128 86 127 84 tfor:TF1672|5end_ATP_synthase,_B_subunit_1786726..1786956_POSITIVE 299 127 89 151 107 tfor:TF2777|5end_hypothetical_protein_2961595..2961825_POSITIVE 299 76 33 99 50 tfor:TF0410|5end_8-amino-7-oxononanoate_synthase_427605..427835_POSITIVE 298 129 81 225 164 tfor:TF1287|5end_hypothetical_protein_1369545..1369775_POSITIVE 294 126 82 117 71 tfor:TF0612|5end_conserved_hypothetical_protein_644066..644296_POSITIVE 287 80 33 155 108 tfor:TF2766|5end_shikimate_kinase_2951619..2951849_POSITIVE 285 103 68 229 184 tfor:TF2525|5end_conserved_hypothetical_protein_2704027..2704257_POSITIVE 277 100 56 79 35 tfor:TF0687|5end_hypothetical_protein_732226..732456_POSITIVE 277 100 56 52 8 tfor:TF0686|5end_hypothetical_protein_732199..732429_POSITIVE 270 127 81 186 152 tfor:TF0690|5end_preprotein_translocase_subunit_735243..735473_POSITIVE 268 128 88 146 106 tfor:TF0198|5end_transposase_related_sequence_221054..221284_POSITIVE 265 70 24 207 161 tfor:TF2380|5end_hemagglutinin_2545012..2545242_POSITIVE 259 120 71 147 84 tfor:TF2790|5end_prismane_protein,_hybrid-cluster_protein_2973397..2973627_POSITIVE 256 151 111 205 151 tfor:TF2891|5end_lipoprotein_3078762..3078992_POSITIVE 252 109 61 95 46 tfor:TF2348|5end_outer_membrane_protein,_possibly_involved_in_nutrient_binding_2511088..2511318_POSITIVE 252 127 84 132 85 tfor:TF1164|5end_two-component_system_sensor_histidine_kinase_1243682..1243912_POSITIVE 249 104 62 62 25 tfor:TF2793|5end_possible_L-lysine_2,3-aminomutase_2977661..2977891_POSITIVE 248 96 53 220 170 tfor:TF2240|5end__tetracycline_resistance_element_mobilization_regulatory_protein_2414723..2414953_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 128 86 56 13 tfor:TF1671|3end_ATP_synthase,_delta_subunit_1786735..1786885_POSITIVE 299 76 33 86 37 tfor:TF0409|3end_conserved_hypothetical_protein_427672..427822_POSITIVE 287 130 81 61 3 tfor:TF2231|3end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_2406248..2406398_POSITIVE 269 127 89 145 100 tfor:TF1483|3end_glycosyltransferase_family_protein_1578825..1578975_POSITIVE 268 128 88 49 9 tfor:TF0196|3end_conserved_hypothetical_protein_221037..221187_POSITIVE 266 128 92 67 25 tfor:TF1926|3end_hypothetical_protein_2074650..2074800_POSITIVE 259 126 83 78 16 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 256 77 32 86 23 tfor:TF0383|3end_alkyl_hydroperoxide_reductase,_subunit_C_406056..406206_POSITIVE 252 127 84 141 94 tfor:TF1163|3end_two-component_system_response_regulator_1243771..1243921_POSITIVE 248 127 89 62 17 tfor:TF1841|3end_hypothetical_protein_1992267..1992417_POSITIVE 245 128 87 59 23 tfor:TF2306|3end_hypothetical_protein_2466915..2467065_POSITIVE 244 105 64 70 20 tfor:TF1985|3end_surface_antigen_BspA_2136905..2137055_POSITIVE 243 128 89 62 14 tfor:TF0985|3end_fructose-bisphosphate_aldolase_1042973..1043123_POSITIVE 243 124 82 55 7 tfor:TF0191|3end_conserved_hypothetical_protein,_possible_Iojap-related_protein_206528..206678_POSITIVE 238 127 81 79 25 tfor:TF1191|3end_possible_transcriptional_regulator_1273979..1274129_POSITIVE 233 127 89 55 12 tfor:TF0369|3end_hypothetical_protein_386186..386336_POSITIVE 232 68 28 46 14 tfor:TF0199|3end_conserved_hypothetical_protein_224064..224214_POSITIVE 231 152 112 101 67 tfor:TF1026|3end_beta-N-acetylglucosaminidase;_beta_lactamase_1085639..1085789_POSITIVE 228 151 115 85 31 tfor:TF1966|3end_conserved_hypothetical_protein_2115476..2115626_POSITIVE 228 70 30 68 21 tfor:TF1449|3end_RNA_polymerase_sigma_factor,_RpoD_1534061..1534211_POSITIVE