Origin IGS: taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatcatgtacccggcagacaattaaccattgacaattaaccattaataaacagccctgaaagggcgagataattcagcccaatgcgaagcgttggggaaaaagcaatatcccgttgggagggctgtaagcctgacataaccattgtcatacatttgcacatccattattaatggtaaacaccaaacagggggtcgcataatttgggttaagcatgggtcatgcatcactctctacatacaaaaaaatgttatatagcatatcgcactacgggagacattaaaaaaatttaccctatctttgtagccttagcacactaaaaagagaatgaatttcgatacatttaacgagtcgtatttgtcgatcccgagaagttattttacggcgaaacaaaatgtccttgattttttaacaaatatta Mask Tandem Repeat Region ================================================ taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattnnnnnnnnnnnnnnnnnnnnnnnnnnnntgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Find is-nt database================================================ Query_seq: TF2201:TF2202|TF2201:TF2202:hypothetical protein:hypothetical protein:->->:2378345..2378821 477 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2201:TF2202|TF2201:TF2202:hypothetical protein:hypothetical protein:->->:2378345..2378821 477 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2201:TF2202|TF2201:TF2202:hypothetical protein:hypothetical protein:->->:2378345..2378821 477 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 17 End: 124 ................ M K D I L F R R K I T S R D R Q I R L V K C I E I H S L F S V L R L Q R ................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 192 End: 374 ............................................................................................................................................................................................... M M H D P C L T Q I M R P P V W C L P L I M D V Q M Y D N G Y V R L T A L P T G Y C F F P N A S H W A E L S R P F R A V ....................................................................................................... Protein_Len: 41 Strand: + Start: 259 End: 381 .................................................................................................................................................................................................................................................................. M C K C M T M V M S G L Q P S Q R D I A F S P T L R I G L N Y L A L S G L F I N G ................................................................................................ Protein_Len: 32 Strand: + Start: 347 End: 442 .......................................................................................................................................................................................................................................................................................................................................................... M I S P F Q G C L L M V N C Q W L I V C R V H D S A C T T H N H ................................... Protein_Len: 37 Strand: - Start: 167 End: 277 ...................................................................................................................................................................... I V N K Q I Y L T I C S G H K V W I I R G G T Q H K G N I I S T C I Y S M ........................................................................................................................................................................................................ Protein_Len: 32 Strand: - Start: 105 End: 200 ........................................................................................................ H A L A V F I P Y I K K I D G T T R Y A I Y C K K T H L S H H M ..................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 2 End: 94 . I N T L F D L V N Q K A T F Y S R P I S L Y S E N F T D F N M ............................................................................................................................................................................................................................................................................................................................................................................................... taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 40 HP_score: -2.3 Tail_Score: -2.64803 Start: 272 End: 288 Full_Region: ggagggctgtaagcc tgaca taaccat tgtca tacatttgcacatcc ...............................................................................................................................................................................................................................................................................tgacataaccattgtca............................................................................................................................................................................................. TransTerm Strand: + Conf: 76 HP_score: -9.1 Tail_Score: -3.74047 Start: 355 End: 367 Full_Region: GGGCTGAATTATCTC GCCCT TTC AGGGC TGTTTATTAATGGTT ..................................................................................................................................................................................................................................................................................................................................................................GCCCTTTCAGGGC.............................................................................................................. TransTerm Strand: + Conf: 56 HP_score: -5.3 Tail_Score: -3.28791 Start: 392 End: 448 Full_Region: ATGGTTAATTGTCAA TGGTTAATTGTCTGCCGGGTACATG ATTCGG CGTGTACCACGCATAACCATTAACCA TTGTCCGAAAGGGCT .......................................................................................................................................................................................................................................................................................................................................................................................................TGGTTAATTGTCTGCCGGGTACATGATTCGGCGTGTACCACGCATAACCATTAACCA............................. Find igs database================================================ Query_seq: TF2201:TF2202|TF2201:TF2202:hypothetical protein:hypothetical protein:->->:2378345..2378821 477 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Intra-Species Hit: Count: 14 Min: 1 Max: 477 Len: 477 Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 0.0 bit: 862.0 Len: 477 Query Start:1 Query End:477 Subject Strand: POSITIVE Subject Start: 1 Subject End: 477 taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaannnnnnnaatgtctcccgtagtgcgatatgctatataacannnnnnngtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta taatatttgttaaaaaatcaaggacattttgtttcgccgtaaaataacttctcgggatcgacaaatacgactcgttaaatgtatcgaaattcattctctttttagtgtgctaaggctacaaagatagggtaaatttttttaatgtctcccgtagtgcgatatgctatataacatttttttgtatgtagagagtgatgcatgacccatgcttaacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggatattgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 2.0E-45 bit: 184.0 Len: 134 Query Start:344 Query End:477 Subject Strand: NEGATIVE Subject Start: 238 Subject End: 367 .......................................................................................................................................................................................................................................................................................................................................................tgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta .......................................................................................................................................................................................................................................................................................................................................................tgaattatctcgccctttcagggctgtttttcaattgttaattgtcaatggttatttg----ccgggtacatgattcggagtgtaccgcgcataaccattaaccattgtccgaaagggctgaaagcccgaatta Subject: tfor_TF0370_TF0371|hypothetical protein:beta-mannosidase precursor|POSITIVE:POSITIVE|[386378,386902]|525 HSP 1 e-value: 2.0E-36 bit: 155.0 Len: 125 Query Start:212 Query End:336 Subject Strand: NEGATIVE Subject Start: 58 Subject End: 183 ...................................................................................................................................................................................................................aacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgg-gatattgctttttccccaacgcttcgc............................................................................................................................................. ...................................................................................................................................................................................................................aacccaaattacgcgatacactgttcggcgtttaccattaacaatggatgtgcgaatgtacgacaatggttacgtcaggcttacagccctcccaacggagatattgctttttccccaacgcttcgc............................................................................................................................................. Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 1.0E-31 bit: 139.0 Len: 125 Query Start:212 Query End:336 Subject Strand: NEGATIVE Subject Start: 77 Subject End: 201 ...................................................................................................................................................................................................................aacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaac-gggatattgctttttccccaacgcttcgc............................................................................................................................................. ...................................................................................................................................................................................................................aacccaaattacgcgatacacttttcggcgtttaccattaataatggatgtgcgaatgtacgacaatggttacgtcaggcttacagccctcccaacggggatattgc-ttttccccaacgcttcgc............................................................................................................................................. HSP 2 e-value: 1.0E-31 bit: 139.0 Len: 74 Query Start:404 Query End:477 Subject Strand: NEGATIVE Subject Start: 273 Subject End: 346 ...................................................................................................................................................................................................................................................................................................................................................................................................................tgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta ...................................................................................................................................................................................................................................................................................................................................................................................................................tgccgggtacatgattcggcgtgtaccacgcataaccattgaccattgtccgaaagggctgaaagcccgaatta Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 137 Query Start:232 Query End:368 Subject Strand: POSITIVE Subject Start: 14 Subject End: 152 .......................................................................................................................................................................................................................................ctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggat---attgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct............................................................................................................. .......................................................................................................................................................................................................................................ctgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgc-cgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct............................................................................................................. Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 2.0E-26 bit: 121.0 Len: 118 Query Start:329 Query End:446 Subject Strand: POSITIVE Subject Start: 335 Subject End: 448 ........................................................................................................................................................................................................................................................................................................................................cgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaac............................... ........................................................................................................................................................................................................................................................................................................................................cgctacgcattgggctgaattattacgccctttcagggctgttttccaattgttaattgtcaatggtta----tttgctgggtacatgattcggcgtgtaccacgcataacaattaac............................... HSP 2 e-value: 5.0E-12 bit: 73.8 Len: 53 Query Start:241 Query End:293 Subject Strand: POSITIVE Subject Start: 51 Subject End: 103 ................................................................................................................................................................................................................................................gtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggctt........................................................................................................................................................................................ ................................................................................................................................................................................................................................................gtttaccattaataatggacgtgcgaatgtacgacaatggttacgtcaggctt........................................................................................................................................................................................ Subject: tfor_TF1585_TF1586|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1690671,1691110]|440 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 127 Query Start:324 Query End:450 Subject Strand: POSITIVE Subject Start: 47 Subject End: 169 ...................................................................................................................................................................................................................................................................................................................................cccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccatt........................... ...................................................................................................................................................................................................................................................................................................................................cccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggtta----tttgccgggtacatgattcgacgtgtaccacgcataacaattgaccatt........................... Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 9.0E-23 bit: 109.0 Len: 128 Query Start:317 Query End:444 Subject Strand: POSITIVE Subject Start: 242 Subject End: 356 ............................................................................................................................................................................................................................................................................................................................ctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccatta................................. ............................................................................................................................................................................................................................................................................................................................cttttttcccaacgcttcgcattgggctgaatgatctcgcccc-------ctatttattaatggttaattgtcaatggtca------tgccgggtacatgattcggcgtgtaacacgcataaccatta................................. HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 57 Query Start:247 Query End:303 Subject Strand: POSITIVE Subject Start: 170 Subject End: 226 ......................................................................................................................................................................................................................................................cattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcc.............................................................................................................................................................................. ......................................................................................................................................................................................................................................................cattaataatgtacgtacgaatgtacgacaagggttacgtcaggcttacagccctcc.............................................................................................................................................................................. Subject: tfor_TF1273_TF1274|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1357523,1357760]|238 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 139 Query Start:214 Query End:352 Subject Strand: POSITIVE Subject Start: 98 Subject End: 238 .....................................................................................................................................................................................................................cccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggat---attgctttttccccaacgcttcgcattgggctgaattatc............................................................................................................................. .....................................................................................................................................................................................................................cccaaattacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccct-ccgccgggatgcgatgccttttcccccaacgcttcgcattgggctgaattatc............................................................................................................................. Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 2.0E-17 bit: 91.7 Len: 90 Query Start:214 Query End:303 Subject Strand: NEGATIVE Subject Start: 454 Subject End: 543 .....................................................................................................................................................................................................................cccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcc.............................................................................................................................................................................. .....................................................................................................................................................................................................................cccaaattacgcgatacactgttcggtgtttaccattaataatggacgtacgaatgtacgacaatggttacgtcaagcttacagccctcc.............................................................................................................................................................................. HSP 2 e-value: 3.0E-13 bit: 77.8 Len: 51 Query Start:318 Query End:368 Subject Strand: NEGATIVE Subject Start: 386 Subject End: 436 .............................................................................................................................................................................................................................................................................................................................tttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct............................................................................................................. .............................................................................................................................................................................................................................................................................................................................tttttccccaacgcttcgcattgggctgaattattatgccctttcagggct............................................................................................................. Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 88 Query Start:390 Query End:477 Subject Strand: POSITIVE Subject Start: 195 Subject End: 276 .....................................................................................................................................................................................................................................................................................................................................................................................................aatggttaattgtc-tgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta .....................................................................................................................................................................................................................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccatta-------cccgaaagggctgaaagcccgaatta HSP 2 e-value: 3.0E-13 bit: 77.8 Len: 123 Query Start:232 Query End:354 Subject Strand: POSITIVE Subject Start: 52 Subject End: 178 .......................................................................................................................................................................................................................................ctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggata--ttgcttttt---ccccaacgcttcgcattgggctgaattatctc........................................................................................................................... .......................................................................................................................................................................................................................................ctgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccct-ccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctc........................................................................................................................... Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 55 Query Start:390 Query End:444 Subject Strand: NEGATIVE Subject Start: 312 Subject End: 367 .....................................................................................................................................................................................................................................................................................................................................................................................................aatggttaattgtc-tgccgggtacatgattcggcgtgtaccacgcataaccatta................................. .....................................................................................................................................................................................................................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcggcgtgtaacacacataaccatta................................. HSP 2 e-value: 8.0E-14 bit: 79.8 Len: 92 Query Start:212 Query End:303 Subject Strand: NEGATIVE Subject Start: 435 Subject End: 526 ...................................................................................................................................................................................................................aacccaaattatgcgaccccctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcc.............................................................................................................................................................................. ...................................................................................................................................................................................................................aacccaaattacgcggtaccctattgggcgtttaccattaataatggacgtacgaatgtacgacaagggttacgtcaggcttacagccctcc.............................................................................................................................................................................. HSP 3 e-value: 1.0E-12 bit: 75.8 Len: 42 Query Start:317 Query End:358 Subject Strand: NEGATIVE Subject Start: 378 Subject End: 419 ............................................................................................................................................................................................................................................................................................................................ctttttccccaacgcttcgcattgggctgaattatctcgccc....................................................................................................................... ............................................................................................................................................................................................................................................................................................................................ctttttccccaacgcttcgcattgggctgaatgatctcgccc....................................................................................................................... Subject: tfor_TF0339_TF0340|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[363808,364032]|225 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 52 Query Start:317 Query End:368 Subject Strand: POSITIVE Subject Start: 101 Subject End: 152 ............................................................................................................................................................................................................................................................................................................................ctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct............................................................................................................. ............................................................................................................................................................................................................................................................................................................................ctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggct............................................................................................................. HSP 2 e-value: 1.0E-9 bit: 65.9 Len: 61 Query Start:241 Query End:301 Subject Strand: POSITIVE Subject Start: 23 Subject End: 83 ................................................................................................................................................................................................................................................gtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccct................................................................................................................................................................................ ................................................................................................................................................................................................................................................gtttaccattaataatgaacgtacgaatgtacgacaagggttacgtcaggcttacagccct................................................................................................................................................................................ Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 3.0E-7 bit: 58.0 Len: 41 Query Start:411 Query End:451 Subject Strand: POSITIVE Subject Start: 53 Subject End: 93 ..........................................................................................................................................................................................................................................................................................................................................................................................................................tacatgattcggcgtgtaccacgcataaccattaaccattg.......................... ..........................................................................................................................................................................................................................................................................................................................................................................................................................tacatggttcggcgtgttccacgcataacaattaaccattg.......................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:350 Query End:385 Subject Strand: POSITIVE Subject Start: 3 Subject End: 38 .............................................................................................................................................................................................................................................................................................................................................................atctcgccctttcagggctgtttattaatggttaat............................................................................................ .............................................................................................................................................................................................................................................................................................................................................................atctcgccctttcagggctgtttttcaatggttaat............................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUUUGUUAAAAAAUCAAGGACAUUUUGUUUCGCCGUAAAAUAACUUCUCGGGAUCGACAAAUACGACUCGUUAAAUGUAUCGAAAUUCAUUCUCUUUUUAGUGUGCUAAGGCUACAAAGAUAGGGUAAAUUUUUUUAAUGUCUCCCGUAGUGCGAUAUGCUAUAUAACAUUUUUUUGUAUGUAGAGAGUGAUGCAUGACCCAUGCUUAACCCAAAUUAUGCGACCCCCUGUUUGGUGUUUACCAUUAAUAAUGGAUGUGCAAAUGUAUGACAAUGGUUAUGUCAGGCUUACAGCCCUCCCAACGGGAUAUUGCUUUUUCCCCAACGCUUCGCAUUGGGCUGAAUUAUCUCGCCCUUUCAGGGCUGUUUAUUAAUGGUUAAUUGUCAAUGGUUAAUUGUCUGCCGGGUACAUGAUUCGGCGUGUACCACGCAUAACCAUUAACCAUUGUCCGAAAGGGCUGAAAGCCCGAAUUA .....................((((.............(((((...(((.(..(((.(((.((......((((.......((((((..........(((((.((((.(....).)))).)))))..(((...(((.....)))....)))......)))))).(((((((.((......))))))))).))))...(((((...))))).............))))).)))..)...))).))))).......(((((..((((....))))...(((((((((((..(((..((((((((.....(((((((.....(((..(((((.((...)).)))))..))).))))))).......))))))))......(..(((((((......)))))))..)..))).((((((((......))))))))..)))))))))))..)))))))))....((((.....))))...... (-127.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCGGGCUUUCAGCCCUUUCGGACAAUGGUUAAUGGUUAUGCGUGGUACACGCCGAAUCAUGUACCCGGCAGACAAUUAACCAUUGACAAUUAACCAUUAAUAAACAGCCCUGAAAGGGCGAGAUAAUUCAGCCCAAUGCGAAGCGUUGGGGAAAAAGCAAUAUCCCGUUGGGAGGGCUGUAAGCCUGACAUAACCAUUGUCAUACAUUUGCACAUCCAUUAUUAAUGGUAAACACCAAACAGGGGGUCGCAUAAUUUGGGUUAAGCAUGGGUCAUGCAUCACUCUCUACAUACAAAAAAAUGUUAUAUAGCAUAUCGCACUACGGGAGACAUUAAAAAAAUUUACCCUAUCUUUGUAGCCUUAGCACACUAAAAAGAGAAUGAAUUUCGAUACAUUUAACGAGUCGUAUUUGUCGAUCCCGAGAAGUUAUUUUACGGCGAAACAAAAUGUCCUUGAUUUUUUAACAAAUAUUA ....((((((((.(((((((..(...(((((((((((.(((.(((..((((((.(......).))))))..)))))).))))))))))).................(((.((((.....))))............((((((((...))))))))................))))..))))))).))))))))......((((.((.......(((..............((((....)))).....(((((..((......((((...(((((...)))))....(((((.((((........))))......((.....)).....)))))...............))))......))..))))).))).........)).))))....((((.((((((.....(((((((((.(((....))).))))......)))))......))))))..))))................. (-126.59) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1043 370 321 118 69 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 1000 368 321 193 146 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 1000 470 421 221 172 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 1000 470 421 169 120 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 969 370 321 80 31 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 967 370 321 218 169 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 961 340 291 223 173 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 951 470 421 183 134 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 925 350 301 181 131 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 890 368 321 189 142 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 886 370 321 192 142 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 868 440 391 184 134 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 819 450 401 168 119 tfor:TF2796|5end_cytochrome_D_ubiquinol_oxidase,_subunit_II_2981269..2981499_POSITIVE 813 370 324 221 170 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 739 368 321 123 66 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 739 368 321 116 59 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 731 358 314 211 167 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 682 368 329 176 137 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 680 349 302 231 178 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 675 447 406 162 122 tfor:TF1260|5end_chaperone_protein_DnaJ_1345325..1345555_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1000 368 321 108 61 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 967 370 321 72 23 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 931 310 261 146 97 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 911 370 324 63 17 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 801 300 251 96 47 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 769 358 315 136 92 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 723 440 394 141 94 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 570 330 281 135 83 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 557 344 305 40 1 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 529 326 281 127 75 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 522 397 356 42 1 tfor:TF1848|3end_conserved_hypothetical_protein_1997944..1998094_POSITIVE 509 423 381 135 87 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 509 423 381 66 18 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 476 416 373 123 79 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 460 368 329 53 9 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 459 424 381 134 87 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 430 428 381 129 81 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 429 302 272 51 17 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 417 428 381 61 6 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 414 470 421 83 41 tfor:TF1796|3end_ABC_transporter,_ATP-binding_protein_1937216..1937366_POSITIVE