Origin IGS: gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaattatctcgccctttcagggctgtttttcaattgttaattgtcaatggttatttgccgggtacatgattcggagtgtaccgcgcataaccattaaccattgtccgaaagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatccgggtttataagaatttttatcccgaactcacataaaaaatgtacctttgcgaaaaaattctaagagggaactcatgagaaaggtacgacagaaatgaaacaatagatataaatcaacacttaaaaaaagagaac Mask Tandem Repeat Region ================================================ gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Find is-nt database================================================ Query_seq: TF2190:TF2191|TF2190:TF2191:conserved hypothetical protein:hypothetical protein:->->:2365707..2366073 367 gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2190:TF2191|TF2190:TF2191:conserved hypothetical protein:hypothetical protein:->->:2365707..2366073 367 gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2190:TF2191|TF2190:TF2191:conserved hypothetical protein:hypothetical protein:->->:2365707..2366073 367 gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 26 End: 115 ......................... M S I V S F L S Y L S H E F P L R I F S Q R Y I F Y V S S G ............................................................................................................................................................................................................................................................ Protein_Len: 41 Strand: + Start: 115 End: 237 .................................................................................................................. M K I L I N P D Y A I E E I R M N L I Q A F S P P N K V R M S I P H P C G M G L K .................................................................................................................................. Protein_Len: 34 Strand: + Start: 219 End: 320 .......................................................................................................................................................................................................................... M R D G A E I I R A F S P F G Q W L M V M R G T L R I M Y P A N N H ............................................... Protein_Len: 60 Strand: - Start: 182 End: 364 ..................................................................................................................................................................................... E S C P G S T S V G G K R S P A S I I R A K L G K P C H N I T I R P V S R I M Y G A F L W Q C N V I S F C G Q F P S I M ... Protein_Len: 31 Strand: - Start: 174 End: 266 ............................................................................................................................................................................. A K L G G F L T R I D I G W G Q P I P S F Y N P S E A R E S M ..................................................................................................... gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 74 HP_score: -9.1 Tail_Score: -3.54919 Start: 244 End: 256 Full_Region: GGGCTGAAATAATTC GGGCT TTC AGCCC TTTCGGACAATGGTT ...................................................................................................................................................................................................................................................GGGCTTTCAGCCC............................................................................................................... Find igs database================================================ Query_seq: TF2190:TF2191|TF2190:TF2191:conserved hypothetical protein:hypothetical protein:->->:2365707..2366073 367 gttctctttttttaagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Intra-Species Hit: Count: 16 Min: 14 Max: 367 Len: 354 Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 0.0 bit: 702.0 Len: 354 Query Start:14 Query End:367 Subject Strand: POSITIVE Subject Start: 14 Subject End: 367 .............aagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca .............aagtgttgatttatatctattgtttcatttctgtcgtacctttctcatgagttccctcttagaattttttcgcaaaggtacattttttatgtgagttcgggataaaaattcttataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 1.0E-95 bit: 351.0 Len: 193 Query Start:127 Query End:319 Subject Strand: POSITIVE Subject Start: 5 Subject End: 197 ..............................................................................................................................ataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataacca................................................ ..............................................................................................................................ataaacccagattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatagggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatcatgtacccggcaaataacca................................................ Subject: tfor_TF3003_TF3004|hypothetical protein:phosphoribosylformylglycinamidine (FGAM) synthase|POSITIVE:POSITIVE|[3220690,3220958]|269 HSP 1 e-value: 1.0E-86 bit: 321.0 Len: 174 Query Start:127 Query End:300 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 174 ..............................................................................................................................ataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatca................................................................... ..............................................................................................................................ataaacccagattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatca................................................................... Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 1.0E-76 bit: 287.0 Len: 157 Query Start:165 Query End:321 Subject Strand: POSITIVE Subject Start: 200 Subject End: 356 ....................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccatt.............................................. ....................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccatt.............................................. Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 6.0E-54 bit: 212.0 Len: 128 Query Start:130 Query End:257 Subject Strand: NEGATIVE Subject Start: 264 Subject End: 393 .................................................................................................................................aacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcg--ataccccacccttgcgggatggggctgaaataattcgggctttcagccct.............................................................................................................. .................................................................................................................................aacccagattccgcaatagaagaaataagaatgaatttaattcaggctttcagccctccgaacaaggtccggatgtcgatataccccacccttgcgggatggggctgaaataattcgggctttcagccct.............................................................................................................. Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 3.0E-53 bit: 210.0 Len: 184 Query Start:128 Query End:311 Subject Strand: NEGATIVE Subject Start: 210 Subject End: 386 ...............................................................................................................................taaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggca........................................................ ...............................................................................................................................taaacccagattcggcaatagaacaaataaggatgaatttaattcaggccttcagcccttcgaacaaggtccggatgtcgatcccccacccttacgggatggggctgaaataattcgggctttcagccctttc-------gggtaatggttatgcgtgttacacgctgaatcatgtacccggca........................................................ Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 6.0E-48 bit: 192.0 Len: 230 Query Start:132 Query End:361 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 235 ...................................................................................................................................cccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccaccctt--gcgggatggggctgaaataattcgggc-tttcagccctttcgga-------caatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagat...... ...................................................................................................................................cccggattccacaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtctggatatcggtatcccacccttgcgcgggatggggctgaaataactagggcttttcagccctttcggacaatagtcaatggttaattgttatgcgtggaacacgccgaaccatgtatttggaaaataaccat-------taaccattgaaaaacagccctgaaagggcgagat...... Subject: tfor_TF0021_TF0022|outer membrane phospholipase A:two-component system sensor histidine kinase|POSITIVE:POSITIVE|[16980,17434]|455 HSP 1 e-value: 9.0E-47 bit: 188.0 Len: 111 Query Start:152 Query End:262 Subject Strand: NEGATIVE Subject Start: 116 Subject End: 226 .......................................................................................................................................................aaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcgg......................................................................................................... .......................................................................................................................................................aaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttacgggatggggctgaaataattcgggctcccggccctttcgg......................................................................................................... Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 1.0E-45 bit: 184.0 Len: 130 Query Start:238 Query End:367 Subject Strand: NEGATIVE Subject Start: 344 Subject End: 477 .............................................................................................................................................................................................................................................taattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccgg----caaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca .............................................................................................................................................................................................................................................taattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatcatgtacccggcagacaattaaccattgacaattaaccattaataaacagccctgaaagggcgagataattca Subject: tfor_TF1585_TF1586|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1690671,1691110]|440 HSP 1 e-value: 1.0E-27 bit: 125.0 Len: 103 Query Start:265 Query End:367 Subject Strand: NEGATIVE Subject Start: 67 Subject End: 169 ........................................................................................................................................................................................................................................................................aatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaacaattgaaaaacagccctgaaagggcgagataattca ........................................................................................................................................................................................................................................................................aatggtcaattgttatgcgtggtacacgtcgaatcatgtacccggcaaataaccattaacaactaacaattggaaaacagccctgaaagggcgtgttaattca HSP 2 e-value: 4.0E-15 bit: 83.8 Len: 54 Query Start:205 Query End:258 Subject Strand: NEGATIVE Subject Start: 198 Subject End: 251 ............................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctt............................................................................................................. ............................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaatatatcgggcttgcagccctt............................................................................................................. Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 7.0E-26 bit: 119.0 Len: 182 Query Start:130 Query End:311 Subject Strand: POSITIVE Subject Start: 178 Subject End: 352 .................................................................................................................................aacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggca........................................................ .................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccctctaaccaagatatgggcatggatcccccacccttacgggatggggctgaaatatatctggctttcagccctttc-------gggtaatggttatgtgtgttacacgccgaatcatgtacccggca........................................................ Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 3.0E-22 bit: 107.0 Len: 62 Query Start:128 Query End:189 Subject Strand: NEGATIVE Subject Start: 299 Subject End: 360 ...............................................................................................................................taaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccctccg.................................................................................................................................................................................. ...............................................................................................................................taaacccggattccgcaatagaagaaataaaaatgaatttaattcaggccttcagccctccg.................................................................................................................................................................................. HSP 2 e-value: 2.0E-17 bit: 91.7 Len: 54 Query Start:205 Query End:258 Subject Strand: NEGATIVE Subject Start: 231 Subject End: 284 ............................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctt............................................................................................................. ............................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaatatgtcgggctttcagccctt............................................................................................................. Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 61 Query Start:126 Query End:186 Subject Strand: NEGATIVE Subject Start: 577 Subject End: 637 .............................................................................................................................tataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... .............................................................................................................................tataaccccggattccgcaatagaagaaataagaatgaatttaattcaggctttcagccct..................................................................................................................................................................................... HSP 2 e-value: 2.0E-7 bit: 58.0 Len: 41 Query Start:271 Query End:311 Subject Strand: NEGATIVE Subject Start: 530 Subject End: 570 ..............................................................................................................................................................................................................................................................................taatggttatgcgcggtacactccgaatcatgtacccggca........................................................ ..............................................................................................................................................................................................................................................................................taatggttatgcgtgttacacgccgaatcatgtacccggca........................................................ Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 6.0E-17 bit: 89.7 Len: 61 Query Start:126 Query End:186 Subject Strand: POSITIVE Subject Start: 8 Subject End: 68 .............................................................................................................................tataaacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... .............................................................................................................................tataaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... HSP 2 e-value: 6.0E-14 bit: 79.8 Len: 48 Query Start:211 Query End:258 Subject Strand: POSITIVE Subject Start: 213 Subject End: 260 ..................................................................................................................................................................................................................ccccacccttgcgggatggggctgaaataattcgggctttcagccctt............................................................................................................. ..................................................................................................................................................................................................................ccccacccttacgggatggggctgaaataattcgggcttccagccctt............................................................................................................. Subject: tfor_TF1654_TF1655|hypothetical protein:long-chain-fatty-acid-CoA ligase|POSITIVE:POSITIVE|[1768766,1769418]|653 HSP 1 e-value: 2.0E-14 bit: 81.8 Len: 57 Query Start:130 Query End:186 Subject Strand: POSITIVE Subject Start: 390 Subject End: 446 .................................................................................................................................aacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... .................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... HSP 2 e-value: 2.0E-7 bit: 58.0 Len: 41 Query Start:271 Query End:311 Subject Strand: POSITIVE Subject Start: 557 Subject End: 597 ..............................................................................................................................................................................................................................................................................taatggttatgcgcggtacactccgaatcatgtacccggca........................................................ ..............................................................................................................................................................................................................................................................................taatggttatgcgtgttacacgccgaatcatgtacccggca........................................................ Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 2.0E-10 bit: 67.9 Len: 61 Query Start:271 Query End:331 Subject Strand: NEGATIVE Subject Start: 298 Subject End: 356 ..............................................................................................................................................................................................................................................................................taatggttatgcgcggtacactccgaatcatgtacccggcaaataaccattgacaattaac.................................... ..............................................................................................................................................................................................................................................................................taatggttatgcgtgttacacgccgaatcatgtacccggc--atgaccattgacaattaac.................................... HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:152 Query End:186 Subject Strand: NEGATIVE Subject Start: 483 Subject End: 517 .......................................................................................................................................................aaataagaatgaatttaattcaggccttcagccct..................................................................................................................................................................................... .......................................................................................................................................................aaataaggatgaatttaattcagaccttcagccct..................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUGUUGAUUUAUAUCUAUUGUUUCAUUUCUGUCGUACCUUUCUCAUGAGUUCCCUCUUAGAAUUUUUUCGCAAAGGUACAUUUUUUAUGUGAGUUCGGGAUAAAAAUUCUUAUAAACCCGGAUUACGCAAUAGAAGAAAUAAGAAUGAAUUUAAUUCAGGCCUUCAGCCCUCCGAACAAGGUCCGGAUGUCGAUACCCCACCCUUGCGGGAUGGGGCUGAAAUAAUUCGGGCUUUCAGCCCUUUCGGACAAUGGUUAAUGGUUAUGCGCGGUACACUCCGAAUCAUGUACCCGGCAAAUAACCAUUGACAAUUAACAAUUGAAAAACAGCCCUGAAAGGGCGAGAUAAUUCA .((.(((((.........((((.((((((..(((.(((((((((....((((((.......))))))....).)))))))).(((((((((((((((((((((((......))))...)))))))).))).)))))))).)))..))))))..))))........))))).))..(((.(((((((((((.(((...((((((((....))).))))).)))....)))))))))))..((((((((((......((((((((((((..((((((((..........))))))..))..)))))))))))).........((....)).....)))))))))).......))). (-108.93) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUUAUCUCGCCCUUUCAGGGCUGUUUUUCAAUUGUUAAUUGUCAAUGGUUAUUUGCCGGGUACAUGAUUCGGAGUGUACCGCGCAUAACCAUUAACCAUUGUCCGAAAGGGCUGAAAGCCCGAAUUAUUUCAGCCCCAUCCCGCAAGGGUGGGGUAUCGACAUCCGGACCUUGUUCGGAGGGCUGAAGGCCUGAAUUAAAUUCAUUCUUAUUUCUUCUAUUGCGUAAUCCGGGUUUAUAAGAAUUUUUAUCCCGAACUCACAUAAAAAAUGUACCUUUGCGAAAAAAUUCUAAGAGGGAACUCAUGAGAAAGGUACGACAGAAAUGAAACAAUAGAUAUAAAUCAACACUU ((((((.....((((.....)))).....(((..(((.((((.((.(((((((((.(((..(((((((........)))))))))).))))))))).)).))))..)))..(((((...(((((...........((((((.(((....))))))))).......(((((((...))))))))))))...))))))))...))))))...((((....(((((((.......((((...(((((....)))))))))................((((((((..(((....)))...(((....))).....))))))))..((....))...))))))).)))).......... (-108.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1147 220 171 208 159 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 1147 220 171 87 38 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 1102 220 171 228 177 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 1094 220 171 201 152 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 992 207 161 74 28 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 804 240 191 107 56 tfor:TF0370|5end_hypothetical_protein_386028..386258_POSITIVE 786 220 175 46 1 tfor:TF2668|5end_hypothetical_protein_2842471..2842701_POSITIVE 770 320 272 204 156 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 770 320 272 173 125 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 752 245 203 43 1 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 685 180 141 231 192 tfor:TF2204|5end_conserved_hypothetical_protein_2378866..2379096_POSITIVE 672 185 141 231 187 tfor:TF1474|5end_6-phosphogluconolactonase_1567342..1567572_POSITIVE 617 158 114 231 187 tfor:TF3004|5end_phosphoribosylformylglycinamidine_(FGAM)_synthase_3220819..3221049_POSITIVE 600 296 251 153 108 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 596 335 291 120 70 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 573 210 161 229 180 tfor:TF2796|5end_cytochrome_D_ubiquinol_oxidase,_subunit_II_2981269..2981499_POSITIVE 531 340 293 111 57 tfor:TF2127|5end_hypothetical_protein_2304038..2304268_POSITIVE 521 291 251 154 114 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 521 291 251 75 35 tfor:TF2664|5end_hypothetical_protein_2841399..2841629_POSITIVE 507 318 272 102 56 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1094 220 171 150 101 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 1055 250 201 53 4 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 830 220 171 51 6 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 773 249 201 127 79 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 599 348 301 88 48 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 518 310 263 54 7 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 497 291 251 129 89 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 495 310 263 113 66 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 484 318 272 48 2 tfor:TF2438|3end_hypothetical_protein_2616157..2616307_POSITIVE 484 291 251 61 14 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 482 320 272 50 2 tfor:TF0468|3end_hypothetical_protein_487188..487338_POSITIVE 478 318 282 42 1 tfor:TF0021|3end_outer_membrane_phospholipase_A_16919..17069_POSITIVE 468 206 161 151 102 tfor:TF0369|3end_hypothetical_protein_386186..386336_POSITIVE 466 300 251 147 95 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 466 300 251 78 26 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 445 318 271 45 1 tfor:TF2817|3end_hypothetical_protein_3007626..3007776_POSITIVE 433 293 251 125 75 tfor:TF2996|3end_hypothetical_protein_3211121..3211271_POSITIVE 430 344 303 147 112 tfor:TF1170|3end_conserved_hypothetical_protein_1252799..1252949_POSITIVE 424 244 201 78 36 tfor:TF1597|3end_TatD_family_protein_1713418..1713568_POSITIVE 423 318 271 83 35 tfor:TF1483|3end_glycosyltransferase_family_protein_1578825..1578975_POSITIVE