Origin IGS: tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgccgatatgaacatccccctttccccccttccaaggaggaatgcggaactgacggctgactgctgtaagagccccgcaggggcgcgttaattcagccca Mask Tandem Repeat Region ================================================ tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Find is-nt database================================================ Query_seq: TF2171:TF2173|TF2171:TF2173:hypothetical protein:hypothetical protein:->->:2345853..2345955 103 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2171:TF2173|TF2171:TF2173:hypothetical protein:hypothetical protein:->->:2345853..2345955 103 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2171:TF2173|TF2171:TF2173:hypothetical protein:hypothetical protein:->->:2345853..2345955 103 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 9 End: 101 ........ M N A P L R G S Y S S Q P S V P H S S L E G G K G G C S Y R R .. tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2171:TF2173|TF2171:TF2173:hypothetical protein:hypothetical protein:->->:2345853..2345955 103 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 4 Min: 1 Max: 103 Len: 103 Subject: tfor_TF2171_TF2173|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345853,2345955]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Subject: tfor_TF2169_TF2171|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345415,2345624]|210 HSP 1 e-value: 1.0E-42 bit: 172.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 108 Subject End: 210 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta tgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Subject: tfor_TF2165_TF2166|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2341983,2342254]|272 HSP 1 e-value: 1.0E-42 bit: 172.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 170 Subject End: 272 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta tgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Subject: tfor_TF0339_TF0340|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[363808,364032]|225 HSP 1 e-value: 3.0E-40 bit: 165.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 123 Subject End: 225 tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta tgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgtgcatatcggcgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGGCUGAAUUAACGCGCCCCUGCGGGGCUCUUACAGCAGUCAGCCGUCAGUUCCGCAUUCCUCCUUGGAAGGGGGGAAAGGGGGAUGUUCAUAUCGGCGUUA ...(((((((.((((..((((.((((((((...((.((.....)).)).)))))))).((((((((.....)))))))).))))..)))).)).))))).... (-42.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGCCGAUAUGAACAUCCCCCUUUCCCCCCUUCCAAGGAGGAAUGCGGAACUGACGGCUGACUGCUGUAAGAGCCCCGCAGGGGCGCGUUAAUUCAGCCCA ((((((.(((......)))((((.((((..(((....))).)))).((((..((.(((((.....)))))...))..)))).)))).)))))).......... (-31.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 688 50 1 210 160 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 663 100 52 54 5 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 662 90 41 211 161 tfor:TF0536|5end_conserved_hypothetical_protein_561917..562147_POSITIVE 640 100 52 209 161 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 611 45 1 191 147 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 596 50 1 213 164 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 589 45 1 231 187 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 580 50 1 149 94 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 580 50 1 142 87 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 569 44 1 203 160 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 555 100 51 221 171 tfor:TF0540|5end_conserved_hypothetical_protein;_possible_surface_protein_567286..567516_POSITIVE 514 100 51 160 107 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 514 100 51 56 3 tfor:TF3106|5end_hypothetical_protein_3338355..3338585_POSITIVE 508 91 55 49 10 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 498 100 51 191 147 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 496 100 51 63 11 tfor:TF1842|5end_hypothetical_protein_1992571..1992801_POSITIVE 490 100 52 56 15 tfor:TF2746|5end_hypothetical_protein_2927996..2928226_POSITIVE 490 48 1 141 94 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 432 78 37 125 74 tfor:TF0187|5end_ferredoxin_202151..202381_POSITIVE 428 39 1 228 190 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 643 100 51 98 46 tfor:TF2651|3end_hypothetical_protein_2822014..2822164_POSITIVE 635 50 1 90 41 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 625 100 51 86 28 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 596 50 1 128 79 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 562 100 51 103 54 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 542 100 51 124 73 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 527 89 41 151 88 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 490 48 1 71 24 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 443 77 37 119 63 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 431 73 37 116 77 tfor:TF1660|3end_hypothetical_protein_1775600..1775750_POSITIVE 411 30 1 82 52 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 396 79 35 55 8 tfor:TF2832|3end_possible_proton/sodium:glutamate_symporter_protein_3023939..3024089_POSITIVE 395 60 19 43 8 tfor:TF0302|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_329611..329761_POSITIVE 394 58 13 79 28 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 389 59 14 73 28 tfor:TF1683|3end_conserved_hypothetical_protein_1796511..1796661_POSITIVE 385 100 54 123 74 tfor:TF0671|3end_histidine_kinase_sensor_protein_716343..716493_POSITIVE 383 30 1 61 32 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 382 100 68 40 8 tfor:TF1483|3end_glycosyltransferase_family_protein_1578825..1578975_POSITIVE 370 48 14 75 24 tfor:TF1173|3end_ABC_transporter,_permease_component_1256651..1256801_POSITIVE 362 59 15 137 99 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE