Origin IGS: taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgccgatatgaacatccccctttccccccttccaaggaggaatgcggaactgacggctgaccgctgtaacagctccgcaggggcgcattaattcagcccaatgtgtagcgaagcgaagcattgggaaaaaagaatacccactcctgcggagggctgtaagcctgatataaaatgttgatttatttaatggtcgagatgttttactta Mask Tandem Repeat Region ================================================ taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Find is-nt database================================================ Query_seq: TF2169:TF2171|TF2169:TF2171:hypothetical protein:hypothetical protein:->->:2345415..2345624 210 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2169:TF2171|TF2169:TF2171:hypothetical protein:hypothetical protein:->->:2345415..2345624 210 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2169:TF2171|TF2169:TF2171:hypothetical protein:hypothetical protein:->->:2345415..2345624 210 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 25 End: 117 ........................ M N Q H F I S G L Q P S A G V G I L F S Q C F A S L H I G L N ............................................................................................. Protein_Len: 31 Strand: + Start: 116 End: 208 ................................................................................................................... M N A P L R S C Y S G Q P S V P H S S L E G G K G G C S Y R R .. Protein_Len: 56 Strand: + Start: 42 End: 209 ......................................... M R L T A L R R S G Y S F F P M L R F A T H W A E L M R P C G A V T A V S R Q F R I P P W K G G K G D V H I G V . Protein_Len: 37 Strand: - Start: 41 End: 151 ........................................ I L S V A R R L L P Y E K K G I S R K A V C Q A S N I R G Q P A T V A T M ........................................................... taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 52 HP_score: -4.5 Tail_Score: -3.27298 Start: 129 End: 165 Full_Region: GAATTAATGCGCCCC TGCGGAGCTGTTACAGC GGTCA GCCGTCAGTTCCGCA TTCCTCCTTGGAAGG ................................................................................................................................TGCGGAGCTGTTACAGCGGTCAGCCGTCAGTTCCGCA............................................. Find igs database================================================ Query_seq: TF2169:TF2171|TF2169:TF2171:hypothetical protein:hypothetical protein:->->:2345415..2345624 210 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Intra-Species Hit: Count: 10 Min: 1 Max: 210 Len: 210 Subject: tfor_TF2169_TF2171|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345415,2345624]|210 HSP 1 e-value: 1.0E-115 bit: 416.0 Len: 210 Query Start:1 Query End:210 Subject Strand: POSITIVE Subject Start: 1 Subject End: 210 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Subject: tfor_TF0365_TF0366|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[383213,383703]|491 HSP 1 e-value: 4.0E-94 bit: 345.0 Len: 206 Query Start:1 Query End:206 Subject Strand: POSITIVE Subject Start: 282 Subject End: 487 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggc.... taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggc.... Subject: tfor_TF0361_TF0362|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[379871,380091]|221 HSP 1 e-value: 9.0E-92 bit: 337.0 Len: 206 Query Start:1 Query End:206 Subject Strand: POSITIVE Subject Start: 12 Subject End: 217 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggc.... taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcatcccttcggagctgttacagtggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggc.... Subject: tfor_TF0358_TF0359|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[376365,377082]|718 HSP 1 e-value: 1.0E-60 bit: 234.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 277 Subject End: 426 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtca............................................................ taagtaaaacatctcgaccattaaataaatcaacattttatatcaggctttcagccctccgcaggagtgggtattcttttcccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtca............................................................ Subject: tfor_TF0349_TF0350|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[370780,370933]|154 HSP 1 e-value: 1.0E-56 bit: 220.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 12 Subject End: 154 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttaca................................................................... taagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca................................................................... Subject: tfor_TF2108_TF2109|hypothetical protein:possible metallo-beta-lactamase family protein|POSITIVE:POSITIVE|[2277750,2278081]|332 HSP 1 e-value: 2.0E-55 bit: 216.0 Len: 152 Query Start:1 Query End:152 Subject Strand: POSITIVE Subject Start: 8 Subject End: 160 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattctttttt-cccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagc.......................................................... taagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagc.......................................................... Subject: tfor_TF2165_TF2166|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2341983,2342254]|272 HSP 1 e-value: 1.0E-53 bit: 210.0 Len: 106 Query Start:105 Query End:210 Subject Strand: POSITIVE Subject Start: 167 Subject End: 272 ........................................................................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta ........................................................................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta HSP 2 e-value: 6.0E-25 bit: 115.0 Len: 58 Query Start:1 Query End:58 Subject Strand: POSITIVE Subject Start: 73 Subject End: 130 taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccct........................................................................................................................................................ taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccct........................................................................................................................................................ Subject: tfor_TF2171_TF2173|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345853,2345955]|103 HSP 1 e-value: 3.0E-42 bit: 172.0 Len: 103 Query Start:108 Query End:210 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 ...........................................................................................................tgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta ...........................................................................................................tgggctgaattaacgcgcccctgcggggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta Subject: tfor_TF0339_TF0340|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[363808,364032]|225 HSP 1 e-value: 4.0E-32 bit: 139.0 Len: 106 Query Start:105 Query End:210 Subject Strand: POSITIVE Subject Start: 120 Subject End: 225 ........................................................................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggcgtta ........................................................................................................cattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgtgcatatcggcgtta Subject: tfor_TF0344_TF0346|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[367024,367187]|164 HSP 1 e-value: 1.0E-29 bit: 131.0 Len: 102 Query Start:105 Query End:206 Subject Strand: POSITIVE Subject Start: 59 Subject End: 160 ........................................................................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaaggggggaaagggggatgttcatatcggc.... ........................................................................................................cattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggc.... HSP 2 e-value: 1.0E-7 bit: 58.0 Len: 29 Query Start:35 Query End:63 Subject Strand: POSITIVE Subject Start: 2 Subject End: 30 ..................................attttatatcaggcttacagccctccgca................................................................................................................................................... ..................................attttatatcaggcttacagccctccgca................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUAAAACAUCUCGACCAUUAAAUAAAUCAACAUUUUAUAUCAGGCUUACAGCCCUCCGCAGGAGUGGGUAUUCUUUUUUCCCAAUGCUUCGCUUCGCUACACAUUGGGCUGAAUUAAUGCGCCCCUGCGGAGCUGUUACAGCGGUCAGCCGUCAGUUCCGCAUUCCUCCUUGGAAGGGGGGAAAGGGGGAUGUUCAUAUCGGCGUUA ...............((((.....(((((.......))))).....(((.(((((..((((((((.(.(.(((((..(((..(((((((....((...))....)))))))..)))..))))).)))))))))))))))...))))))).((((...(((((.(.((((((((.....)))))))).).)))))........)))).... (-74.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGCCGAUAUGAACAUCCCCCUUUCCCCCCUUCCAAGGAGGAAUGCGGAACUGACGGCUGACCGCUGUAACAGCUCCGCAGGGGCGCAUUAAUUCAGCCCAAUGUGUAGCGAAGCGAAGCAUUGGGAAAAAAGAAUACCCACUCCUGCGGAGGGCUGUAAGCCUGAUAUAAAAUGUUGAUUUAUUUAAUGGUCGAGAUGUUUUACUUA ............(((((((...(((.....((((((.(((((...((((((.((((((((.....)))))..))).)))))).(((...(((.......((((((((...((...))...)))))))).......))).))).)))))..))))))....)))...(((...(((((......)))))....)))..)))))))...... (-62.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 890 110 61 84 34 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 762 127 81 113 67 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 762 127 81 106 60 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 719 140 93 222 175 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 715 174 131 229 184 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 697 150 103 189 142 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 689 129 81 202 143 tfor:TF1406|5end_transposase_1481758..1481988_POSITIVE 682 100 51 79 30 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 663 209 161 56 7 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 658 90 43 79 33 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 658 90 43 214 168 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 656 200 151 214 164 tfor:TF0536|5end_conserved_hypothetical_protein_561917..562147_POSITIVE 648 150 105 229 184 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 640 209 161 211 163 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 636 119 76 46 2 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 611 100 51 71 21 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 610 127 81 179 143 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 602 95 51 46 3 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 599 95 51 46 3 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 587 90 43 213 166 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 711 180 131 115 64 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 665 210 162 101 50 tfor:TF2651|3end_hypothetical_protein_2822014..2822164_POSITIVE 663 127 81 72 26 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 611 100 51 140 90 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 588 200 152 77 23 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 587 90 43 71 24 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 581 209 162 105 58 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 575 90 43 109 62 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 561 209 162 126 77 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 532 100 52 146 97 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 532 140 93 64 22 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 522 196 151 151 106 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 458 90 43 139 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 447 190 144 119 63 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 447 139 91 64 7 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 427 180 138 116 69 tfor:TF1660|3end_hypothetical_protein_1775600..1775750_POSITIVE 413 169 124 64 16 tfor:TF2770|3end_conserved_hypothetical_protein_2956639..2956789_POSITIVE 413 165 121 79 22 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 412 74 43 151 122 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 410 167 121 92 36 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE