Origin IGS: taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctatccgccacgataaacaagcagcttcgtcgcgttgcggtaacgagtatcgggcaattcattcgtcggggaacagattcagaatgcaaaatcaattcatttcacatgcaatatttttttacgcaaaaatcattataggtaaatatacgtatatccactaaaattattgatacaaggggaaagcttgttgaatattta Mask Tandem Repeat Region ================================================ taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Find is-nt database================================================ Query_seq: TF2142:TF2143|TF2142:TF2143:hypothetical protein:S-adenosylmethionine:tRNA ribosyltransferase-isomerase:->->:2319273..2319470 198 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2142:TF2143|TF2142:TF2143:hypothetical protein:S-adenosylmethionine:tRNA ribosyltransferase-isomerase:->->:2319273..2319470 198 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2142:TF2143|TF2142:TF2143:hypothetical protein:S-adenosylmethionine:tRNA ribosyltransferase-isomerase:->->:2319273..2319470 198 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 104 End: 196 ....................................................................................................... M I L H S E S V P R R M N C P I L V T A T R R S C L F I V A D .. Protein_Len: 41 Strand: - Start: 64 End: 186 ............................................................... L S K Q T F F Y Q M H F S N I K C E S D T G R R I F Q G I S T V A V R R L Q K N M ............ Protein_Len: 52 Strand: - Start: 41 End: 196 ........................................ H I Y T Y K G I I I K A Y F F I A H S I F Q N Q M R F R N G S S H I A R Y E N G C R S S A A Q K D H R M .. taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2142:TF2143|TF2142:TF2143:hypothetical protein:S-adenosylmethionine:tRNA ribosyltransferase-isomerase:->->:2319273..2319470 198 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Intra-Species Hit: Count: 1 Min: 1 Max: 198 Len: 198 Subject: tfor_TF2142_TF2143|hypothetical protein:S-adenosylmethionine:tRNA ribosyltransferase-isomerase|POSITIVE:POSITIVE|[2319273, 2319470]|198 HSP 1 e-value: 6.0E-96 bit: 351.0 Len: 198 Query Start:1 Query End:198 Subject Strand: POSITIVE Subject Start: 1 Subject End: 198 taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtnnnnnnntattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag taaatattcaacaagctttccccttgtatcaataattttagtggatatacgtatatttacctataatgatttttgcgtaaaaaaatattgcatgtgaaatgaattgattttgcattctgaatctgttccccgacgaatgaattgcccgatactcgttaccgcaacgcgacgaagctgcttgtttatcgtggcggatag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUUCAACAAGCUUUCCCCUUGUAUCAAUAAUUUUAGUGGAUAUACGUAUAUUUACCUAUAAUGAUUUUUGCGUAAAAAAAUAUUGCAUGUGAAAUGAAUUGAUUUUGCAUUCUGAAUCUGUUCCCCGACGAAUGAAUUGCCCGAUACUCGUUACCGCAACGCGACGAAGCUGCUUGUUUAUCGUGGCGGAUAG ..........(((((.......))))).............((((((((....))))))))...((((((...(((.((((.........(.(((..((((......))))..))).).......((((......))))...)))).)))...))))))((((..(((((..((((.....)))).))))))))).... (-33.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAUCCGCCACGAUAAACAAGCAGCUUCGUCGCGUUGCGGUAACGAGUAUCGGGCAAUUCAUUCGUCGGGGAACAGAUUCAGAAUGCAAAAUCAAUUCAUUUCACAUGCAAUAUUUUUUUACGCAAAAAUCAUUAUAGGUAAAUAUACGUAUAUCCACUAAAAUUAUUGAUACAAGGGGAAAGCUUGUUGAAUAUUUA ......(((.(((((.....(((((........))))).........))))))))....((((((((.(....).)))...))))).....(((((...((((...(((.............)))........((((.(((....))).)))).........................))))....)))))....... (-31.86) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 647 169 121 230 184 tfor:TF2307|5end_two-component_system_sensor_histidine_kinase/response_2462618..2462848_POSITIVE 381 176 131 62 4 tfor:TF2898|5end_conserved_hypothetical_protein_3087061..3087291_POSITIVE 376 189 141 125 72 tfor:TF0463|5end_precorrin-6Y_C5,15-methyltransferase_479015..479245_POSITIVE 371 180 133 73 27 tfor:TF2225|5end_CTn_excision_protein_2394935..2395165_POSITIVE 362 188 144 114 53 tfor:TF1891|5end_hypothetical_protein_2043389..2043619_POSITIVE 360 178 131 194 141 tfor:TF1835|5end_possible_dihydroxy-acid_dehydratase_1984201..1984431_POSITIVE 358 161 124 45 3 tfor:TF1041|5end_dihydroorotate_dehydrogenase,_catalytic_subunit_1102029..1102259_POSITIVE 358 168 123 169 124 tfor:TF0098|5end_permease_108103..108333_POSITIVE 353 190 144 68 14 tfor:TF1071|5end_hypothetical_protein_1140969..1141199_POSITIVE 352 178 131 199 154 tfor:TF0970|5end_2-keto-3-deoxygluconate_kinase_1020396..1020626_POSITIVE 350 178 131 212 170 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 346 162 130 230 200 tfor:TF0697|5end_glycerol-3-phosphate_cytidyltransferase_740667..740897_POSITIVE 342 188 144 57 14 tfor:TF2573|5end_50S_ribosomal_protein_L15_2743310..2743540_POSITIVE 340 150 101 216 168 tfor:TF1315|5end_purine_nucleoside_phosphorylase_1390236..1390466_POSITIVE 340 190 142 196 136 tfor:TF1179|5end_two-component_sensor_histidine_kinase_1266057..1266287_POSITIVE 338 180 131 86 21 tfor:TF2849|5end_histidinol-phosphate_transaminase_3037288..3037518_POSITIVE 338 169 126 134 75 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 335 168 121 227 164 tfor:TF2601|5end_conserved_hypothetical_protein_2774721..2774951_POSITIVE 335 178 142 179 128 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 335 167 123 62 7 tfor:TF2228|5end_CTn_excision_protein_2395899..2396129_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 189 141 125 72 tfor:TF0462|3end_possible_cobalamin_biosynthesis_precorrin-3_methylase_479095..479245_POSITIVE 371 183 143 67 24 tfor:TF0145|3end_glutamine_synthetase_154718..154868_POSITIVE 358 161 124 54 12 tfor:TF1040|3end_dihydroorotate_dehydrogenase,_electron_transfer_subunit_1102118..1102268_POSITIVE 353 167 123 149 101 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 338 180 131 107 42 tfor:TF2848|3end_cobalamin_biosynthesis_protein_3037389..3037539_POSITIVE 338 169 126 101 42 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 335 168 121 135 72 tfor:TF2600|3end_ribose-phosphate_pyrophosphokinase_2774709..2774859_POSITIVE 330 169 123 143 100 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 325 169 122 61 6 tfor:TF2266|3end_transfer_region-related_protein,_TraQ_2434949..2435099_POSITIVE 322 178 135 149 106 tfor:TF0410|3end_8-amino-7-oxononanoate_synthase_428869..429019_POSITIVE 318 177 131 76 28 tfor:TF3121|3end_nicotinate_phosphoribosyltransferase_3356124..3356274_POSITIVE 318 169 126 77 22 tfor:TF2659|3end_conserved_hypothetical_protein_2833055..2833205_POSITIVE 315 188 141 60 16 tfor:TF0388|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_408846..408996_POSITIVE 313 177 134 43 2 tfor:TF2042|3end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2202356..2202506_POSITIVE 313 76 32 75 25 tfor:TF0270|3end_transposase_element_298074..298224_POSITIVE 311 166 124 47 3 tfor:TF1717|3end_DNA_mismatch_repair_protein_1840494..1840644_POSITIVE 310 178 131 128 89 tfor:TF2379|3end_phosphoribosylformylglycinamidine_cyclo-ligase_(AIR_synthase)_2545092..2545242_POSITIVE 309 178 131 61 12 tfor:TF2900|3end_conserved_hypothetical_protein_3090029..3090179_POSITIVE 308 164 126 143 99 tfor:TF2685|3end_iron-regulated_ABC-type_transporter_membrane_component_2862725..2862875_POSITIVE 308 180 133 99 45 tfor:TF2657|3end_conserved_hypothetical_protein_2830873..2831023_POSITIVE