Origin IGS: atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctatccaactactacaaacgttttaagtgcaaaggtgtggaacctttgtgaacagcctactattttttgtataattattttcggcattattaagttgctatagtttttgaaaataccagaagcgttaatcttcctatagtcaatatattgacgttctaaaaagaataaaacctatacttttaacaacaccttatactttagtcccaaaaatgatttgttgaagtaggtagcacatcaggatatttccacgagtat Mask Tandem Repeat Region ================================================ atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Find is-nt database================================================ Query_seq: TF2135:TF2136|TF2135:TF2136:conserved hypothetical protein:conserved hypothetical protein:->->:2311696..2311950 255 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2135:TF2136|TF2135:TF2136:conserved hypothetical protein:conserved hypothetical protein:->->:2311696..2311950 255 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2135:TF2136|TF2135:TF2136:conserved hypothetical protein:conserved hypothetical protein:->->:2311696..2311950 255 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 112 End: 252 ............................................................................................................... M D Y R K I N A S G I F K N Y S N L I M P K I I I Q K I V G C S Q R F H T F A L K T F V V V G * ... atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2135:TF2136|TF2135:TF2136:conserved hypothetical protein:conserved hypothetical protein:->->:2311696..2311950 255 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Intra-Species Hit: Count: 1 Min: 1 Max: 255 Len: 255 Subject: tfor_TF2135_TF2136|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2311696,2311950]|255 HSP 1 e-value: 1.0E-142 bit: 505.0 Len: 255 Query Start:1 Query End:255 Subject Strand: POSITIVE Subject Start: 1 Subject End: 255 atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag atactcgtggaaatatcctgatgtgctacctacttcaacaaatcatttttgggactaaagtataaggtgttgttaaaagtataggttttattctttttagaacgtcaatatattgactataggaagattaacgcttctggtattttcaaaaactatagcaacttaataatgccgaaaataattatacaaaaaatagtaggctgttcacaaaggttccacacctttgcacttaaaacgtttgtagtagttggatag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUACUCGUGGAAAUAUCCUGAUGUGCUACCUACUUCAACAAAUCAUUUUUGGGACUAAAGUAUAAGGUGUUGUUAAAAGUAUAGGUUUUAUUCUUUUUAGAACGUCAAUAUAUUGACUAUAGGAAGAUUAACGCUUCUGGUAUUUUCAAAAACUAUAGCAACUUAAUAAUGCCGAAAAUAAUUAUACAAAAAAUAGUAGGCUGUUCACAAAGGUUCCACACCUUUGCACUUAAAACGUUUGUAGUAGUUGGAUAG .((((((.(((....)))))).)))(((.(((((...((((((...(((((((......(((((((((((((((((...........(((.((((((((....(((((....)))))..)))))))).))).(((..((((..........)))).)))...)))))))))))........))))))....(((((....)))))..(((((((.....)))))))..))))))).))))))))))).))).... (-51.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAUCCAACUACUACAAACGUUUUAAGUGCAAAGGUGUGGAACCUUUGUGAACAGCCUACUAUUUUUUGUAUAAUUAUUUUCGGCAUUAUUAAGUUGCUAUAGUUUUUGAAAAUACCAGAAGCGUUAAUCUUCCUAUAGUCAAUAUAUUGACGUUCUAAAAAGAAUAAAACCUAUACUUUUAACAACACCUUAUACUUUAGUCCCAAAAAUGAUUUGUUGAAGUAGGUAGCACAUCAGGAUAUUUCCACGAGUAU .(((((.............((((.....((((((((.....)))))))))))).((...(((((((......(((((((((.((.((((.((((.((.....(((((((.......)))))))(((((..........(((((....))))).((((....))))..............)))))..)).)))).....)))).)).)))))))))....)))))))...))......)))))............. (-46.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 274 140 94 73 38 tfor:TF0914|5end_conserved_hypothetical_protein;_possible_ketosteroid_isomerase-related_protein_955332..955562_POSITIVE 262 47 1 92 42 tfor:TF0818|5end_bifunctional_protein:__aspartokinase_I;_homoserine_dehydrogenase_876002..876232_POSITIVE 245 241 206 53 19 tfor:TF2336|5end_conserved_hypothetical_protein_2496169..2496399_POSITIVE 243 231 196 180 149 tfor:TF1457|5end_conserved_hypothetical_protein;_possible_transporter_1543229..1543459_POSITIVE 239 49 5 80 31 tfor:TF1455|5end_conserved_hypothetical_protein_1540688..1540918_POSITIVE 237 238 201 214 176 tfor:TF2126|5end_S-adenosylhomocysteine_hydrolase_2302501..2302731_POSITIVE 234 43 6 47 13 tfor:TF1957|5end_penicillin_tolerance_protein;_possible_isoprene_biosynthesis_related_protein_2107789..2108019_POSITIVE 230 55 15 112 54 tfor:TF2027|5end_conserved_hypothetical_protein_2183863..2184093_POSITIVE 230 249 206 220 168 tfor:TF0789|5end_preprotein_translocase,_secDF_family_841293..841523_POSITIVE 229 240 199 44 8 tfor:TF3142|5end_conserved_hypothetical_protein_3376357..3376587_POSITIVE 228 31 4 181 148 tfor:TF2986|5end_conserved_hypothetical_protein;_possible_glycosyltransferase_3201818..3202048_POSITIVE 228 240 198 207 146 tfor:TF0690|5end_preprotein_translocase_subunit_735243..735473_POSITIVE 226 56 16 231 190 tfor:TF2581|5end_50S_ribosomal_protein_L17_2748814..2749044_POSITIVE 225 55 17 202 166 tfor:TF1532|5end_potassium_uptake_system_protein_1637881..1638111_POSITIVE 222 88 43 59 12 tfor:TF2961|5end_type_I_restriction_enzyme_3171887..3172117_POSITIVE 222 227 198 50 12 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 222 236 195 212 168 tfor:TF2588|5end_coproporphryninogen_III_oxidase_2752117..2752347_POSITIVE 222 236 195 51 7 tfor:TF2587|5end_hypothetical_protein_2751956..2752186_POSITIVE 222 228 194 228 196 tfor:TF0032|5end_sugar_phosphate_permease,_major_facilitator_superfamily_27911..28141_POSITIVE 221 244 201 193 130 tfor:TF0674|5end_transposase_fragment_717199..717429_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 102 66 51 16 tfor:TF0184|3end_dihydroneopterin_aldolase_196062..196212_POSITIVE 237 238 201 98 60 tfor:TF2125|3end_conserved_hypothetical_protein_2302465..2302615_POSITIVE 234 43 6 51 17 tfor:TF1956|3end_hypothetical_protein_2107873..2108023_POSITIVE 233 232 193 79 33 tfor:TF0673|3end_conserved_hypothetical_protein_717355..717505_POSITIVE 232 250 210 100 57 tfor:TF0598|3end_hypothetical_protein_629500..629650_POSITIVE 227 226 193 146 109 tfor:TF0674|3end_transposase_fragment_717431..717581_POSITIVE 227 139 97 69 30 tfor:TF0667|3end_conserved_hypothetical_protein_712378..712528_POSITIVE 222 227 198 50 12 tfor:TF2819|3end_N-acetyl-gamma-glutamylphosphate_reductase_3009956..3010106_POSITIVE 222 236 195 79 35 tfor:TF2586|3end_conserved_hypothetical_protein_2752064..2752214_POSITIVE 219 46 5 140 101 tfor:TF3072|3end_conserved_hypothetical_protein_3296083..3296233_POSITIVE 219 175 131 101 51 tfor:TF0383|3end_alkyl_hydroperoxide_reductase,_subunit_C_406056..406206_POSITIVE 215 250 210 126 83 tfor:TF1184|3end_hypothetical_protein_1269311..1269461_POSITIVE 215 250 210 126 83 tfor:TF1183|3end_hypothetical_protein_1268728..1268878_POSITIVE 215 224 190 97 52 tfor:TF0851|3end_helicase_SNF2_family_903168..903318_POSITIVE 214 250 210 82 39 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 213 145 103 78 23 tfor:TF2155|3end_transposase_2331096..2331246_POSITIVE 212 239 191 82 26 tfor:TF2412|3end_outer_membrane_protein_2590393..2590543_POSITIVE 212 180 131 103 61 tfor:TF1472|3end_acyl-ACP_thioesterase_1566337..1566487_POSITIVE 212 26 5 23 1 tfor:TF1215|3end_haloacid_dehalogenase-like_hydrolase_1303216..1303366_POSITIVE 211 129 83 87 43 tfor:TF0928|3end_conserved_hypothetical_protein_965656..965806_POSITIVE