Origin IGS: gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacacaatgatccgttaaagaacatgcctgcgatcacacagagcgattccttttaaaaatatcgctaaacaaaacacaattaaacattaaaaacagcccttcgggcgttgggttgtttgattgttgagttgtcgaattgttggctgatagctgactgcttaaaagctgatagctgaccactgtaacagctccgaaggggtgcgttaattcagcccaatgtgtagcgaagcgaagcgttggggaaaaaagaatacccactcctgcggagggctgtaaaccttatataaaatattaatttatttaatggtcgagatgttttacttaaacaaac Mask Tandem Repeat Region ================================================ gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Find is-nt database================================================ Query_seq: TF2108:TF2109|TF2108:TF2109:hypothetical protein:possible metallo-beta-lactamase family protein:->->:2277750..2278081 332 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2108:TF2109|TF2108:TF2109:hypothetical protein:possible metallo-beta-lactamase family protein:->->:2277750..2278081 332 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2108:TF2109|TF2108:TF2109:hypothetical protein:possible metallo-beta-lactamase family protein:->->:2277750..2278081 332 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 36 End: 125 ................................... M N I L Y K V Y S P P Q E W V F F F P Q R F A S L H I G L N ............................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 124 End: 249 ........................................................................................................................... M N A P L R S C Y S G Q L S A F K Q S A I S Q Q F D N S T I K Q P N A R R A V F N V ................................................................................... Protein_Len: 60 Strand: + Start: 74 End: 295 ......................................................................... M G I L F S P T L R F A T H W A E L T H P F G A V T V V S Y Q L L S S Q L S A N N S T T Q Q S N N P T P E G L F L M F N ..................................... Protein_Len: 40 Strand: - Start: 40 End: 159 ....................................... Y K I Y P K C G E A P T P I R K E G V S R K A V C Q A S N V C G K P A T V T T M ............................................................................................................................................................................. gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 74 HP_score: -4.5 Tail_Score: -4.946 Start: 225 End: 238 Full_Region: AATCAAACAACCCAA CGCCC GAA GGGCTG TTTTTAATGTTTAAT ................................................................................................................................................................................................................................CGCCCGAAGGGCTG.............................................................................................. TransTerm Strand: + Conf: 75 HP_score: -8.2 Tail_Score: -4.07993 Start: 226 End: 236 Full_Region: ATCAAACAACCCAAC GCCC GAA GGGC TGTTTTTAATGTTTA .................................................................................................................................................................................................................................GCCCGAAGGGC................................................................................................ Find igs database================================================ Query_seq: TF2108:TF2109|TF2108:TF2109:hypothetical protein:possible metallo-beta-lactamase family protein:->->:2277750..2278081 332 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Intra-Species Hit: Count: 7 Min: 1 Max: 332 Len: 332 Subject: tfor_TF2108_TF2109|hypothetical protein:possible metallo-beta-lactamase family protein|POSITIVE:POSITIVE|[2277750,2278081]|332 HSP 1 e-value: 0.0 bit: 658.0 Len: 332 Query Start:1 Query End:332 Subject Strand: POSITIVE Subject Start: 1 Subject End: 332 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagctatcagcttttaagcagtcagctatcagccaacaattcgacaactcaacaatcaaacaacccaacgcccgaagggctgtttttaatgtttaattgtgttttgtttagcgatatttttaaaaggaatcgctctgtgtgatcgcaggcatgttctttaacggatcattgtgtca Subject: tfor_TF0358_TF0359|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[376365,377082]|718 HSP 1 e-value: 3.0E-83 bit: 309.0 Len: 236 Query Start:1 Query End:236 Subject Strand: POSITIVE Subject Start: 270 Subject End: 515 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggt--cagctatcagcttttaagcagtcagctatcagcc---------aacaattcgacaactcaacaatcaaacaacccaacgcccgaagggc................................................................................................ gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggctttcagccctccgcaggagtgggtattcttttc-ccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcacagctatcagttttcaagcagtcagctatcagccaacagtcaaaacaattcaacaactcaacaatcaaacaactcaacgcccgaagggc................................................................................................ Subject: tfor_TF0365_TF0366|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[383213,383703]|491 HSP 1 e-value: 2.0E-66 bit: 254.0 Len: 160 Query Start:1 Query End:160 Subject Strand: POSITIVE Subject Start: 275 Subject End: 433 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagc............................................................................................................................................................................ gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattc-ttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagc............................................................................................................................................................................ Subject: tfor_TF0361_TF0362|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[379871,380091]|221 HSP 1 e-value: 4.0E-64 bit: 246.0 Len: 160 Query Start:1 Query End:160 Subject Strand: POSITIVE Subject Start: 5 Subject End: 163 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagc............................................................................................................................................................................ gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattc-ttttcccccaacgctttgcttcgctacacattgggctgaattaacgcatcccttcggagctgttacagtggtcagc............................................................................................................................................................................ Subject: tfor_TF0349_TF0350|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[370780,370933]|154 HSP 1 e-value: 1.0E-58 bit: 228.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 5 Subject End: 154 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca..................................................................................................................................................................................... gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattc-ttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca..................................................................................................................................................................................... Subject: tfor_TF2169_TF2171|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345415,2345624]|210 HSP 1 e-value: 4.0E-55 bit: 216.0 Len: 153 Query Start:8 Query End:160 Subject Strand: POSITIVE Subject Start: 1 Subject End: 152 .......taagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttacagtggtcagc............................................................................................................................................................................ .......taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattctttttt-cccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttacagcggtcagc............................................................................................................................................................................ Subject: tfor_TF2165_TF2166|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2341983,2342254]|272 HSP 1 e-value: 2.0E-19 bit: 97.6 Len: 65 Query Start:1 Query End:65 Subject Strand: POSITIVE Subject Start: 66 Subject End: 130 gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccct........................................................................................................................................................................................................................................................................... gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccct........................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-9 bit: 63.9 Len: 48 Query Start:113 Query End:160 Subject Strand: POSITIVE Subject Start: 167 Subject End: 214 ................................................................................................................cattgggctgaattaacgcaccccttcggagctgttacagtggtcagc............................................................................................................................................................................ ................................................................................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagc............................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUGUUUAAGUAAAACAUCUCGACCAUUAAAUAAAUUAAUAUUUUAUAUAAGGUUUACAGCCCUCCGCAGGAGUGGGUAUUCUUUUUUCCCCAACGCUUCGCUUCGCUACACAUUGGGCUGAAUUAACGCACCCCUUCGGAGCUGUUACAGUGGUCAGCUAUCAGCUUUUAAGCAGUCAGCUAUCAGCCAACAAUUCGACAACUCAACAAUCAAACAACCCAACGCCCGAAGGGCUGUUUUUAAUGUUUAAUUGUGUUUUGUUUAGCGAUAUUUUUAAAAGGAAUCGCUCUGUGUGAUCGCAGGCAUGUUCUUUAACGGAUCAUUGUGUCA ...(((((.....)))))....((((.(((.(((((.......))))).))))))).(((((..((((.(((.(((.((......(((..(((((.(....((...))....).)))))..)))...)).))).))).)))))))))((((((((((.((((...(((....(((.....)))...))).........(((((..((.((((.(((((....(((((((...)))).))).....))))).))))))..)))))))))...........(((((((.((.(((((....)))))))..)))))))....))))))))))... (-81.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACACAAUGAUCCGUUAAAGAACAUGCCUGCGAUCACACAGAGCGAUUCCUUUUAAAAAUAUCGCUAAACAAAACACAAUUAAACAUUAAAAACAGCCCUUCGGGCGUUGGGUUGUUUGAUUGUUGAGUUGUCGAAUUGUUGGCUGAUAGCUGACUGCUUAAAAGCUGAUAGCUGACCACUGUAACAGCUCCGAAGGGGUGCGUUAAUUCAGCCCAAUGUGUAGCGAAGCGAAGCGUUGGGGAAAAAAGAAUACCCACUCCUGCGGAGGGCUGUAAACCUUAUAUAAAAUAUUAAUUUAUUUAAUGGUCGAGAUGUUUUACUUAAACAAAC ........(((.((((((((...((.(((.((((((......((((((.............))))))..((((..((((((((((((.......((((((....)).))))...)))))))))).))..)))).).))))).))))).((((((.(.(((....))).).)))))).........(((((((((.(((((((.((..((((..((((((((...((...))...)))))))).......)))))).))))))).))))..))))).........................)))))))))))...(((((.....)))))... (-92.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 971 120 71 86 37 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 754 160 113 205 157 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 717 138 91 115 69 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 717 138 91 108 62 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 702 140 91 204 148 tfor:TF1406|5end_transposase_1481758..1481988_POSITIVE 655 160 113 231 184 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 649 109 61 80 33 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 645 127 83 46 2 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 639 148 101 222 175 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 629 160 111 191 142 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 622 100 52 81 35 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 622 100 52 216 170 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 614 109 61 72 24 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 587 100 52 215 168 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 587 100 52 158 111 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 584 159 113 203 157 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 569 100 52 164 118 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 560 109 61 54 6 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 532 138 96 115 74 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 525 109 61 54 6 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 720 139 91 76 28 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 655 160 113 85 38 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 614 109 61 141 93 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 587 100 52 73 26 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 582 100 52 111 64 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 570 235 193 43 1 tfor:TF1657|3end_lauroyl/myristoyl_acyltransferase_1773407..1773557_POSITIVE 563 109 61 147 99 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 532 138 96 45 4 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 505 148 101 64 22 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 475 230 181 66 9 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE 468 109 71 40 4 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 454 216 173 74 31 tfor:TF1816|3end_hypothetical_protein_1957974..1958124_POSITIVE 454 216 173 91 48 tfor:TF1817|3end_hypothetical_protein_1957991..1958141_POSITIVE 443 140 91 54 2 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 425 236 201 56 22 tfor:TF1715|3end_thermolysin;_zinc_metalloprotease_1837202..1837352_POSITIVE 416 214 175 106 63 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 415 220 173 75 16 tfor:TF2832|3end_possible_proton/sodium:glutamate_symporter_protein_3023939..3024089_POSITIVE 405 188 145 99 45 tfor:TF0531|3end_hypothetical_protein_557469..557619_POSITIVE 400 94 52 132 90 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 376 238 193 75 30 tfor:TF1174|3end_ABC_transporter,_permease_component_1257896..1258046_POSITIVE