Origin IGS: aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaattccgtttttttttatctttggtgcaaagatagtatattttatattacattagtgttgctttgctgttttttgttatttctgtttcgtatcttttccaaaaaacgtatctttgtacaaagtagttcgttaatgaataaatttt Mask Tandem Repeat Region ================================================ aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Find is-nt database================================================ Query_seq: TF2094:TF2095|TF2094:TF2095:6-phosphofructokinase:5'-nucleotidase precursor:->->:2263585..2263730 146 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2094:TF2095|TF2094:TF2095:6-phosphofructokinase:5'-nucleotidase precursor:->->:2263585..2263730 146 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2094:TF2095|TF2094:TF2095:6-phosphofructokinase:5'-nucleotidase precursor:->->:2263585..2263730 146 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 4 End: 96 ... M Y S L T N Y F V Q R Y V F W K R Y E T E I T K N S K A T L M .................................................. Protein_Len: 30 Strand: + Start: 12 End: 101 ........... M N E L L C T K I R F L E K I R N R N N K K Q Q S N T N V I ............................................. Protein_Len: 36 Strand: - Start: 2 End: 109 . F K N M L S S S Q V F I R K K S F I R F L F L L F C C L L V L T I Y F M ..................................... aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2094:TF2095|TF2094:TF2095:6-phosphofructokinase:5'-nucleotidase precursor:->->:2263585..2263730 146 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagataaaaaaaaacggaattt Intra-Species Hit: Count: 1 Min: 1 Max: 129 Len: 129 Subject: tfor_TF2094_TF2095|6-phosphofructokinase:5'-nucleotidase precursor|POSITIVE:POSITIVE|[2263585,2263730]|146 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagat................. aaaatttattcattaacgaactactttgtacaaagatacgttttttggaaaagatacgaaacagaaataacaaaaaacagcaaagcaacactaatgtaatataaaatatactatctttgcaccaaagat................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUUUAUUCAUUAACGAACUACUUUGUACAAAGAUACGUUUUUUGGAAAAGAUACGAAACAGAAAUAACAAAAAACAGCAAAGCAACACUAAUGUAAUAUAAAAUAUACUAUCUUUGCACCAAAGAU .......................(((((..((((((((.((((((((.......................))))))))......(((.......)))..............))))))))...))))).. (-16.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCUUUGGUGCAAAGAUAGUAUAUUUUAUAUUACAUUAGUGUUGCUUUGCUGUUUUUUGUUAUUUCUGUUUCGUAUCUUUUCCAAAAAACGUAUCUUUGUACAAAGUAGUUCGUUAAUGAAUAAAUUUU ..(((((.(((((((((((((......((((((...)))))).....))).((((((((..(.................)..)))))))).)))))))))))))))..(((((....)))))....... (-21.23) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 266 70 25 163 113 tfor:TF0650|5end_serine/threonine-protein_kinase_691442..691672_POSITIVE 247 60 11 175 129 tfor:TF0215|5end_tRNA/rRNA_methyltransferase,_TrmH_family_241463..241693_POSITIVE 229 48 2 72 29 tfor:TF2545|5end_hypothetical_protein_2729053..2729283_POSITIVE 218 120 71 222 165 tfor:TF2185|5end_outer_membrane_protein,_TonB_dependent_receptor_2357262..2357492_POSITIVE 218 58 17 190 152 tfor:TF1423|5end_cell_division_protein_(ftsW)_1505942..1506172_POSITIVE 217 85 50 103 68 tfor:TF0397|5end_ferredoxin-like_protein_417596..417826_POSITIVE 216 90 45 119 64 tfor:TF2278|5end_conserved_hypothetical_protein_2438662..2438892_POSITIVE 206 58 16 117 82 tfor:TF0095|5end_methyltransferase_105893..106123_POSITIVE 205 50 21 141 113 tfor:TF0307|5end_conserved_hypothetical_protein_332783..333013_POSITIVE 198 89 43 114 73 tfor:TF0892|5end_conserved_hypothetical_protein_940836..941066_POSITIVE 194 74 31 57 12 tfor:TF2845|5end_hypothetical_protein_3034414..3034644_POSITIVE 194 120 75 138 101 tfor:TF0412|5end_conserved_hypothetical_protein_429735..429965_POSITIVE 193 90 46 118 66 tfor:TF2913|5end_conserved_hypothetical_protein_3107910..3108140_POSITIVE 193 58 30 158 123 tfor:TF1245|5end_LemA_protein_1328822..1329052_POSITIVE 193 72 34 166 123 tfor:TF0228|5end_probable_integral_outer_membrane_protein_253851..254081_POSITIVE 192 67 24 61 2 tfor:TF0110|5end_unsaturated_glucuronyl_hydrolase_116832..117062_POSITIVE 191 72 32 114 75 tfor:TF1626|5end_nicotinate-nucleotide_adenylyltransferase_1739416..1739646_POSITIVE 191 87 41 185 137 tfor:TF0370|5end_hypothetical_protein_386028..386258_POSITIVE 191 87 41 90 42 tfor:TF0369|5end_hypothetical_protein_385933..386163_POSITIVE 189 49 5 185 129 tfor:TF2780|5end_hypothetical_protein_2966060..2966290_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 266 70 25 79 29 tfor:TF0649|3end_protein_serine/threonine_phosphatase_691438..691588_POSITIVE 217 85 50 103 68 tfor:TF0396|3end_conserved_hypothetical_protein_417676..417826_POSITIVE 216 90 45 102 47 tfor:TF2276|3end_conserved_hypothetical_protein_2438725..2438875_POSITIVE 198 70 39 48 11 tfor:TF2356|3end_PAP2_superfamily_protein_2522409..2522559_POSITIVE 194 120 75 78 41 tfor:TF0411|3end_methyltransferase_429755..429905_POSITIVE 193 90 46 128 76 tfor:TF2912|3end_hypothetical_protein_3108000..3108150_POSITIVE 193 58 30 69 34 tfor:TF1244|3end_hypothetical_protein_1328813..1328963_POSITIVE 193 72 34 82 39 tfor:TF0226|3end_hypothetical_protein_253847..253997_POSITIVE 189 49 5 132 76 tfor:TF2781|3end_hypothetical_protein_2966087..2966237_POSITIVE 188 49 8 142 102 tfor:TF2285|3end_hypothetical_protein_2441024..2441174_POSITIVE 187 59 16 140 104 tfor:TF0064|3end_conserved_hypothetical_protein_70412..70562_POSITIVE 184 82 45 100 65 tfor:TF2033|3end_conserved_hypothetical_protein_2193321..2193471_POSITIVE 183 67 24 50 2 tfor:TF2054|3end_UDP-N-acetylglucosamine_2-epimerase_2219535..2219685_POSITIVE 183 57 32 40 16 tfor:TF0142|3end_subtilisin-like_serine_proteinase_151265..151415_POSITIVE 180 93 57 36 8 tfor:TF2481|3end_possible_thiol_protease/hemagglutinin_PrtT_2658583..2658733_POSITIVE 180 57 18 70 34 tfor:TF0555|3end_Bacteroides_aerotolerance_protein,_BatB_588144..588294_POSITIVE 179 92 56 110 57 tfor:TF2373|3end_UDP-3-O-acyl-GlcNAc_deacetylase_2539806..2539956_POSITIVE 177 65 22 87 45 tfor:TF0718|3end_methylenetetrahydrofolate_dehydrogenase;_methenyltetrahydrofolate_cyclohydrolase_769980..770130_POSITIVE 175 43 10 140 104 tfor:TF0550|3end_stationary-phase-upregulated_protein,_UstA_582924..583074_POSITIVE 174 80 37 103 57 tfor:TF2151|3end_uridine_kinase_2324789..2324939_POSITIVE