Origin IGS: ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacaaaaggtattttttgattgcttttaattgtctcatgatcctcgtggtcgtttattcgtccgatggagatggatttcggaaagattaaagctgttgtataattaacttgag Mask Tandem Repeat Region ================================================ ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Find is-nt database================================================ Query_seq: TF2093:TF2094|TF2093:TF2094:conserved hypothetical protein:6-phosphofructokinase:->->:2262463..2262576 114 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2093:TF2094|TF2093:TF2094:conserved hypothetical protein:6-phosphofructokinase:->->:2262463..2262576 114 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2093:TF2094|TF2093:TF2094:conserved hypothetical protein:6-phosphofructokinase:->->:2262463..2262576 114 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Predict ORF larger than 30AA ================================================ ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2093:TF2094|TF2093:TF2094:conserved hypothetical protein:6-phosphofructokinase:->->:2262463..2262576 114 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: tfor_TF2093_TF2094|conserved hypothetical protein:6- phosphofructokinase|POSITIVE:POSITIVE|[2262463, 2262576]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca ctcaagttaattatacaacagctttaatctttccgaaatccatctccatcggacgaataaacgaccacgaggatcatgagacaattaaaagcaatcaaaaaataccttttgtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCAAGUUAAUUAUACAACAGCUUUAAUCUUUCCGAAAUCCAUCUCCAUCGGACGAAUAAACGACCACGAGGAUCAUGAGACAAUUAAAAGCAAUCAAAAAAUACCUUUUGUCA ....................((((((((..(..((..((((.((....(((..........)))....))))))..))..)..))).)))))...(((((......)))))... (-12.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACAAAAGGUAUUUUUUGAUUGCUUUUAAUUGUCUCAUGAUCCUCGUGGUCGUUUAUUCGUCCGAUGGAGAUGGAUUUCGGAAAGAUUAAAGCUGUUGUAUAAUUAACUUGAG ...((((((....))))))...(((((.((((...((..(((((((.(((.((......)).))).....)).)))))...))..))))))))).((((......))))..... (-20.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 76 32 200 152 tfor:TF2773|5end_S-adenosylmethionine_tRNA_ribosyltransferase_2958117..2958347_POSITIVE 310 86 49 69 27 tfor:TF2551|5end_30S_ribosomal_protein_S10_2734433..2734663_POSITIVE 308 80 32 183 133 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 297 58 30 29 3 tfor:TF0169|5end_possible_CRISPR-associated_protein_Cas1_178550..178780_POSITIVE 293 74 33 57 21 tfor:TF3110|5end_conserved_hypothetical_protein_with_helicase_domain_3342714..3342944_POSITIVE 292 71 31 191 140 tfor:TF1246|5end_protease,_with_chaperone_function_1329390..1329620_POSITIVE 289 79 33 125 60 tfor:TF2205|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2379055..2379285_POSITIVE 289 70 21 173 120 tfor:TF0403|5end_TPR_repeat_protein_422600..422830_POSITIVE 287 78 38 160 122 tfor:TF2946|5end_conserved_hypothetical_protein;_possible_ATPase_3151750..3151980_POSITIVE 286 59 18 125 81 tfor:TF2378|5end_peptide_chain_release_factor_1_2542682..2542912_POSITIVE 286 78 45 202 175 tfor:TF1956|5end_hypothetical_protein_2107632..2107862_POSITIVE 286 68 27 83 26 tfor:TF0312|5end_outer_membrane_protein_336473..336703_POSITIVE 282 77 34 67 23 tfor:TF1164|5end_two-component_system_sensor_histidine_kinase_1243682..1243912_POSITIVE 281 90 42 165 107 tfor:TF1435|5end_acetate_coenzyme_A_transferase_1519574..1519804_POSITIVE 275 76 31 66 22 tfor:TF1955|5end_amino_acid_exporter,_possible_threonine_efflux_protein_2106952..2107182_POSITIVE 275 76 34 41 1 tfor:TF1548|5end_thiophene_and_furan_oxidation_protein_1652501..1652731_POSITIVE 274 78 31 96 37 tfor:TF2992|5end_hexapeptide_transferase_family_protein_3209042..3209272_POSITIVE 271 66 21 173 133 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 270 75 31 170 130 tfor:TF0763|5end_chromate_transport_protein_809675..809905_POSITIVE 268 53 16 91 49 tfor:TF3135|5end_hypothetical_protein_3369821..3370051_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 310 86 49 48 6 tfor:TF2550|3end_translation_elongation_factor_G_2734492..2734642_POSITIVE 293 74 33 66 30 tfor:TF3109|3end_hypothetical_protein_3342803..3342953_POSITIVE 289 79 33 112 47 tfor:TF2204|3end_conserved_hypothetical_protein_2379122..2379272_POSITIVE 289 70 21 102 49 tfor:TF0402|3end_conserved_hypothetical_protein_422609..422759_POSITIVE 287 78 38 132 94 tfor:TF2945|3end_DNA-directed_RNA_polymerase_beta'_subunit_3151802..3151952_POSITIVE 286 59 18 69 25 tfor:TF2377|3end_orotidine_5'_phosphate_decarboxylase_2542706..2542856_POSITIVE 286 78 45 139 112 tfor:TF1955|3end_amino_acid_exporter,_possible_threonine_efflux_protein_2107649..2107799_POSITIVE 282 77 34 76 32 tfor:TF1163|3end_two-component_system_response_regulator_1243771..1243921_POSITIVE 281 90 42 106 48 tfor:TF1434|3end_transketolase_1519595..1519745_POSITIVE 274 78 31 81 22 tfor:TF2991|3end_conserved_hypothetical_protein_3209107..3209257_POSITIVE 271 66 21 96 56 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 268 53 16 103 61 tfor:TF3133|3end_conserved_hypothetical_protein_3369913..3370063_POSITIVE 268 80 31 59 15 tfor:TF2434|3end_conserved_hypothetical_protein_2614363..2614513_POSITIVE 265 67 33 39 4 tfor:TF0969|3end_oxidoreductase_1020439..1020589_POSITIVE 259 79 41 66 31 tfor:TF2848|3end_cobalamin_biosynthesis_protein_3037389..3037539_POSITIVE 259 80 33 126 69 tfor:TF2181|3end_conserved_hypothetical_protein_2354963..2355113_POSITIVE 258 80 32 62 19 tfor:TF0007|3end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_3599..3749_POSITIVE 248 70 27 136 90 tfor:TF2635|3end_ABC_transporter,_ATP-binding_protein/permease_component_2806101..2806251_POSITIVE 246 74 39 151 104 tfor:TF0306|3end_transglutaminase-like_enzyme/cysteine_protease_332749..332899_POSITIVE 245 75 33 112 73 tfor:TF3159|3end_conserved_hypothetical_protein;_possible_transcriptional_regulator_3395611..3395761_POSITIVE