Origin IGS: attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacgcgcaaaaatataacgaaatcttttttaacccagattccgcaatagaagaaataagaatgaatttaattcaggctttcagccctccgaacaaggtccggatgtcgatataccccacccttgcgggatggggctgaaataattcgggctttcagccctcgtaaaccccgcagaggcggtgagttgttgaattgttgagatgtttatttgtttagttgttgatttgttggctctgctgattgctgaatgctgactgctgattgcagaaagagccctgaaagggcgagataattcagcccaatgcgaagcgttggggaaaaaggcatcgcatcccggcggagggctgtaagcctgacgtaaccattgtcgtgcattcgtacgtccatgattaatggtaaacgccgaacagtgtatcgcgtaat Mask Tandem Repeat Region ================================================ attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Find is-nt database================================================ Query_seq: TF2047:TF2048|TF2047:TF2048:hypothetical protein:conserved hypothetical protein:->->:2210359..2210782 424 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2047:TF2048|TF2047:TF2048:hypothetical protein:conserved hypothetical protein:->->:2210359..2210782 424 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2047:TF2048|TF2047:TF2048:hypothetical protein:conserved hypothetical protein:->->:2210359..2210782 424 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 1 End: 216 M T R Y T V R R L P L I M D V R M H D N G Y V R L T A L R R D A M P F S P T L R I G L N Y L A L S G L F L Q S A V S I Q ................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 9 End: 341 ........ M H C S A F T I N H G R T N A R Q W L R Q A Y S P P P G C D A F F P N A S H W A E L S R P F R A L S A I S S Q H S A I S ................................................................................... Protein_Len: 35 Strand: + Start: 250 End: 354 ......................................................................................................................................................................................................................................................... M R G L R G L K A R I I S A P S R K G G V Y R H P D L V R R A E S L N ...................................................................... Protein_Len: 38 Strand: - Start: 289 End: 402 ................................................................................................................................................................................................................................................................................................ G W G A L T P Y I S M R V K N P P S F A Q I L N M R I E E I A S D P N F F M ...................... Protein_Len: 31 Strand: - Start: 77 End: 169 ............................................................................ L G G G P H S A K K G L A E C Q A S N D R G K L A R E A I L M ............................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 34 End: 135 ................................. D H V Y S H V V I T V D P K C G E A P I R H R K G W R K A N P Q I M ................................................................................................................................................................................................................................................................................................. attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 71 HP_score: -9.1 Tail_Score: -3.53733 Start: 139 End: 151 Full_Region: GGGCTGAATTATCTC GCCCT TTC AGGGC TCTTTCTGCAATCAG ..........................................................................................................................................GCCCTTTCAGGGC................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 71 HP_score: -10.4 Tail_Score: -2.88544 Start: 290 End: 310 Full_Region: CCCGAATTATTTCAG CCCCATCC CGCAA GGGTGGGG TATATCGACATCCGG .................................................................................................................................................................................................................................................................................................CCCCATCCCGCAAGGGTGGGG.................................................................................................................. Find igs database================================================ Query_seq: TF2047:TF2048|TF2047:TF2048:hypothetical protein:conserved hypothetical protein:->->:2210359..2210782 424 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagccaacaaatcaacaactaaacaaataaacatctcaacaattcaacaactcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Intra-Species Hit: Count: 20 Min: 1 Max: 424 Len: 424 Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 1.0E-105 bit: 383.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagc....................................................................................................................................................................................................................................... attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagcaatcagcagagc....................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-100 bit: 365.0 Len: 184 Query Start:241 Query End:424 Subject Strand: POSITIVE Subject Start: 241 Subject End: 424 ................................................................................................................................................................................................................................................tcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca ................................................................................................................................................................................................................................................tcaccgcctctgcggggtttacgagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaaagatttcgttatatttttgcgcgtca Subject: tfor_TF1273_TF1274|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1357523,1357760]|238 HSP 1 e-value: 9.0E-69 bit: 262.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 103 Subject End: 238 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatc................................................................................................................................................................................................................................................................................................ attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgccttttcccccaacgcttcgcattgggctgaattatc................................................................................................................................................................................................................................................................................................ Subject: tfor_TF0339_TF0340|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[363808,364032]|225 HSP 1 e-value: 2.0E-57 bit: 224.0 Len: 173 Query Start:1 Query End:173 Subject Strand: POSITIVE Subject Start: 1 Subject End: 173 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcag........................................................................................................................................................................................................................................................... attacgcgatacacggttcggcgtttaccattaataatgaacgtacgaatgtacgacaagggttacgtcaggcttacagcccttcgccgagacgcgatgcctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcag........................................................................................................................................................................................................................................................... Subject: tfor_TF3003_TF3004|hypothetical protein:phosphoribosylformylglycinamidine (FGAM) synthase|POSITIVE:POSITIVE|[3220690,3220958]|269 HSP 1 e-value: 3.0E-56 bit: 220.0 Len: 130 Query Start:264 Query End:393 Subject Strand: POSITIVE Subject Start: 44 Subject End: 171 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat--cgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatctgggtt............................... Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 1.0E-55 bit: 218.0 Len: 181 Query Start:1 Query End:181 Subject Strand: NEGATIVE Subject Start: 357 Subject End: 538 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgc-ctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcagcattcagc................................................................................................................................................................................................................................................... attacgcgatacactgttcggtgtttaccattaataatggacgtacgaatgtacgacaatggttacgtcaagcttacagccctccgccgagatgcgatgctttttttccccaacgcttcgcattgggctgaattattatgccctttcagggcttttacagcagtcagctatcagtattcagc................................................................................................................................................................................................................................................... HSP 2 e-value: 7.0E-17 bit: 89.7 Len: 57 Query Start:337 Query End:393 Subject Strand: NEGATIVE Subject Start: 12 Subject End: 68 ................................................................................................................................................................................................................................................................................................................................................agggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... ................................................................................................................................................................................................................................................................................................................................................agggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggtt............................... HSP 3 e-value: 3.0E-13 bit: 77.8 Len: 47 Query Start:264 Query End:310 Subject Strand: NEGATIVE Subject Start: 213 Subject End: 259 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtgggg.................................................................................................................. .......................................................................................................................................................................................................................................................................agggctggaagcccgaattatttcagccccatcccgtaagggtgggg.................................................................................................................. Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 7.0E-54 bit: 212.0 Len: 130 Query Start:264 Query End:393 Subject Strand: NEGATIVE Subject Start: 130 Subject End: 257 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat--cgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatccgggtt............................... Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 7.0E-54 bit: 212.0 Len: 130 Query Start:264 Query End:393 Subject Strand: NEGATIVE Subject Start: 8 Subject End: 135 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccctatcccgcaagggtggggtat--cgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatctgggtt............................... Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 4.0E-46 bit: 186.0 Len: 142 Query Start:1 Query End:142 Subject Strand: NEGATIVE Subject Start: 378 Subject End: 519 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccc.......................................................................................................................................................................................................................................................................................... attacgcggtaccctattgggcgtttaccattaataatggacgtacgaatgtacgacaagggttacgtcaggcttacagccctccgccgaaatgtgaagcctttttccccaacgcttcgcattgggctgaatgatctcgccc.......................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-17 bit: 91.7 Len: 58 Query Start:336 Query End:393 Subject Strand: NEGATIVE Subject Start: 178 Subject End: 235 ...............................................................................................................................................................................................................................................................................................................................................gagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... ...............................................................................................................................................................................................................................................................................................................................................gagggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggtt............................... HSP 3 e-value: 2.0E-8 bit: 61.9 Len: 47 Query Start:264 Query End:310 Subject Strand: NEGATIVE Subject Start: 259 Subject End: 305 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtgggg.................................................................................................................. .......................................................................................................................................................................................................................................................................agggctgaaagccagatatatttcagccccatcccgtaagggtgggg.................................................................................................................. Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 3.0E-44 bit: 180.0 Len: 130 Query Start:264 Query End:393 Subject Strand: POSITIVE Subject Start: 257 Subject End: 384 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgtaagggtgggggat--cgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtt............................... HSP 2 e-value: 3.0E-41 bit: 170.0 Len: 135 Query Start:4 Query End:138 Subject Strand: POSITIVE Subject Start: 42 Subject End: 178 ...acgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgccttttt--ccccaacgcttcgcattgggctgaattatctc.............................................................................................................................................................................................................................................................................................. ...acgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctc.............................................................................................................................................................................................................................................................................................. Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 3.0E-44 bit: 180.0 Len: 142 Query Start:1 Query End:142 Subject Strand: POSITIVE Subject Start: 137 Subject End: 283 attacgcgatacactgttcggcgtttaccatta-----atcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccc.......................................................................................................................................................................................................................................................................................... attacgcgatacactgttgggcgtttaccattacattaataatgtacgtacgaatgtacgacaagggttacgtcaggcttacagccctccgccgagacgcgatgccttttttcccaacgcttcgcattgggctgaatgatctcgccc.......................................................................................................................................................................................................................................................................................... Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 4.0E-43 bit: 176.0 Len: 120 Query Start:1 Query End:120 Subject Strand: POSITIVE Subject Start: 29 Subject End: 149 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggc-ttacagccctccgccgggatgcgatgcctttttccccaacgcttcgc................................................................................................................................................................................................................................................................................................................ attacgcgatacattgttcggcgtttaccattaataatggacgtgcgaatgtacgacaatggttacgtcaggcttttcagccctccgccgagatgcgatgcctttttccccaacgcttcgc................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 40 Query Start:113 Query End:152 Subject Strand: POSITIVE Subject Start: 335 Subject End: 374 ................................................................................................................cgcttcgcattgggctgaattatctcgccctttcagggct................................................................................................................................................................................................................................................................................ ................................................................................................................cgctacgcattgggctgaattattacgccctttcagggct................................................................................................................................................................................................................................................................................ Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 1.0E-37 bit: 159.0 Len: 95 Query Start:264 Query End:358 Subject Strand: NEGATIVE Subject Start: 200 Subject End: 292 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaa.................................................................. .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtgggg--tatcgacatccggaccttgttcggagggctgaaggcctgaattaaa.................................................................. HSP 2 e-value: 9.0E-29 bit: 129.0 Len: 85 Query Start:1 Query End:85 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 194 attacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctcc................................................................................................................................................................................................................................................................................................................................................... attacgcgatacacttttcggcgtttaccattaataatggatgtgcgaatgtacgacaatggttacgtcaggcttacagccctcc................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 9.0E-32 bit: 139.0 Len: 126 Query Start:271 Query End:396 Subject Strand: POSITIVE Subject Start: 115 Subject End: 240 ..............................................................................................................................................................................................................................................................................aaagcccgaattatttcagccccatcccgc--aagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaa............................ ..............................................................................................................................................................................................................................................................................aaagccctagttatttcagccccatcccgcgcaagggtggg--ataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaa............................ Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 139 Query Start:14 Query End:152 Subject Strand: POSITIVE Subject Start: 232 Subject End: 368 .............ctgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgc-cgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct................................................................................................................................................................................................................................................................................ .............ctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcccaacgggat---attgctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct................................................................................................................................................................................................................................................................................ Subject: tfor_TF1181_TF1183|methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1268052,1268530]|479 HSP 1 e-value: 5.0E-24 bit: 113.0 Len: 77 Query Start:76 Query End:152 Subject Strand: NEGATIVE Subject Start: 382 Subject End: 458 ...........................................................................acagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct................................................................................................................................................................................................................................................................................ ...........................................................................acagcccttcgccgagatgcgatgcctttttccccaacgcttcgcattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................ HSP 2 e-value: 8.0E-20 bit: 99.6 Len: 62 Query Start:337 Query End:398 Subject Strand: NEGATIVE Subject Start: 166 Subject End: 227 ................................................................................................................................................................................................................................................................................................................................................agggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaaaa.......................... ................................................................................................................................................................................................................................................................................................................................................agggctgaaggcctgaattaaattcattcttatttcttctattgcggaatccgggataaaaa.......................... Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 8.0E-20 bit: 99.6 Len: 58 Query Start:63 Query End:120 Subject Strand: POSITIVE Subject Start: 765 Subject End: 822 ..............................................................ttacgtcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgc................................................................................................................................................................................................................................................................................................................ ..............................................................ttacgtcaggcttacagccctccgccgagatgcgatgccttttttcccaacgcttcgc................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-12 bit: 75.8 Len: 46 Query Start:348 Query End:393 Subject Strand: POSITIVE Subject Start: 949 Subject End: 994 ...........................................................................................................................................................................................................................................................................................................................................................cctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... ...........................................................................................................................................................................................................................................................................................................................................................cctgaattaaattcattcttatttcttctatcgcggaatccgggtt............................... Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 60 Query Start:334 Query End:393 Subject Strand: POSITIVE Subject Start: 299 Subject End: 358 .............................................................................................................................................................................................................................................................................................................................................cggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt............................... .............................................................................................................................................................................................................................................................................................................................................cggagggctgaaggcctgaattaaattcatttttatttcttctattgcggaatccgggtt............................... HSP 2 e-value: 5.0E-15 bit: 83.8 Len: 50 Query Start:264 Query End:313 Subject Strand: POSITIVE Subject Start: 232 Subject End: 281 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat............................................................................................................... .......................................................................................................................................................................................................................................................................agggctgaaagcccgacatatttcagccccatcccgcaagggtggggtat............................................................................................................... Subject: tfor_TF0344_TF0346|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[367024,367187]|164 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 106 Query Start:68 Query End:173 Subject Strand: POSITIVE Subject Start: 10 Subject End: 112 ...................................................................tcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcag........................................................................................................................................................................................................................................................... ...................................................................tcaggcttacagccctccgcaagga---gattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcag........................................................................................................................................................................................................................................................... Subject: tfor_TF1585_TF1586|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1690671,1691110]|440 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 50 Query Start:264 Query End:313 Subject Strand: POSITIVE Subject Start: 199 Subject End: 248 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat............................................................................................................... .......................................................................................................................................................................................................................................................................agggctgcaagcccgatatatttcagccccatcccgcaagggtggggtat............................................................................................................... HSP 2 e-value: 7.0E-11 bit: 69.9 Len: 55 Query Start:98 Query End:152 Subject Strand: POSITIVE Subject Start: 37 Subject End: 91 .................................................................................................tgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct................................................................................................................................................................................................................................................................................ .................................................................................................tgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggct................................................................................................................................................................................................................................................................................ Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 7.0E-8 bit: 60.0 Len: 50 Query Start:264 Query End:313 Subject Strand: POSITIVE Subject Start: 1026 Subject End: 1075 .......................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat............................................................................................................... .......................................................................................................................................................................................................................................................................agggctgaaagcccggtatatttcagccccatcccgtaaggatggggtat............................................................................................................... HSP 2 e-value: 4.0E-6 bit: 54.0 Len: 43 Query Start:113 Query End:155 Subject Strand: POSITIVE Subject Start: 888 Subject End: 930 ................................................................................................................cgcttcgcattgggctgaattatctcgccctttcagggctctt............................................................................................................................................................................................................................................................................. ................................................................................................................cgctgcgcattgggctgaattaatacgccctttcagggctctt............................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUACGCGAUACACUGUUCGGCGUUUACCAUUAAUCAUGGACGUACGAAUGCACGACAAUGGUUACGUCAGGCUUACAGCCCUCCGCCGGGAUGCGAUGCCUUUUUCCCCAACGCUUCGCAUUGGGCUGAAUUAUCUCGCCCUUUCAGGGCUCUUUCUGCAAUCAGCAGUCAGCAUUCAGCAAUCAGCAGAGCCAACAAAUCAACAACUAAACAAAUAAACAUCUCAACAAUUCAACAACUCACCGCCUCUGCGGGGUUUACGAGGGCUGAAAGCCCGAAUUAUUUCAGCCCCAUCCCGCAAGGGUGGGGUAUAUCGACAUCCGGACCUUGUUCGGAGGGCUGAAAGCCUGAAUUAAAUUCAUUCUUAUUUCUUCUAUUGCGGAAUCUGGGUUAAAAAAGAUUUCGUUAUAUUUUUGCGCGUCA ...(((((.......(((((((((...((((.....)))))))).)))))....((.(((((.....((((((((.((((((((((((((.((((((((((............(((..((.....(((((((......((((.....)))).((..((((.....))))..)).))))))).....))..)))..................................................(((((..((((((((....(.(((((((((..........))))))))))))))))))..))))))))..)))).)))))))........)))))))))).)))))))).......))))).))........(((.(((((((((..........))))))))).)))......))))).. (-144.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACGCGCAAAAAUAUAACGAAAUCUUUUUUAACCCAGAUUCCGCAAUAGAAGAAAUAAGAAUGAAUUUAAUUCAGGCUUUCAGCCCUCCGAACAAGGUCCGGAUGUCGAUAUACCCCACCCUUGCGGGAUGGGGCUGAAAUAAUUCGGGCUUUCAGCCCUCGUAAACCCCGCAGAGGCGGUGAGUUGUUGAAUUGUUGAGAUGUUUAUUUGUUUAGUUGUUGAUUUGUUGGCUCUGCUGAUUGCUGAAUGCUGACUGCUGAUUGCAGAAAGAGCCCUGAAAGGGCGAGAUAAUUCAGCCCAAUGCGAAGCGUUGGGGAAAAAGGCAUCGCAUCCCGGCGGAGGGCUGUAAGCCUGACGUAACCAUUGUCGUGCAUUCGUACGUCCAUGAUUAAUGGUAAACGCCGAACAGUGUAUCGCGUAAU ..(((((..........(((...((((((...................))))))..................((((((((.((((((((((........((((...((((((.(((((.((.(((((((((((((((((((..........))))))))).)))....))))))).)).)).).)))))))).....((.(((((((.(((..(((((.((..(....)..))...)))))..(((((((.((..((((.....)))).....((((.....)))).))...)))))))(((((((...)))))))..))).))))))).))..)))))))))))))).))))))))))).((((((((((((.((......)).))))).)))))))..((((......))))..)))))... (-143.81) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1040 310 262 217 167 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 1040 310 262 217 167 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 1039 120 71 65 16 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 1039 120 71 218 169 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 1039 120 71 161 112 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 1022 130 81 167 118 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 1006 340 291 231 183 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 1003 150 101 114 65 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 963 120 71 184 135 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 941 350 301 92 45 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 911 150 101 197 148 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 847 150 101 188 138 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 798 90 41 191 141 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 797 150 101 114 54 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 794 120 71 182 135 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 794 370 321 52 3 tfor:TF0820|5end_hypothetical_protein_879656..879886_POSITIVE 781 310 261 48 2 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 746 270 225 193 148 tfor:TF0449|5end_transposase_467891..468121_POSITIVE 699 145 101 214 170 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 699 150 101 121 61 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1097 130 81 124 75 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 1039 120 71 134 85 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 1039 120 71 76 27 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 933 140 91 58 9 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 860 140 91 49 1 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 849 360 313 50 3 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 771 90 46 145 100 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 746 270 225 133 88 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 680 378 331 53 6 tfor:TF0819|3end_phosphoglycerate_mutase_879729..879879_POSITIVE 660 390 341 76 27 tfor:TF0029|3end_hypothetical_protein_25062..25212_POSITIVE 619 150 102 80 23 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 554 120 71 141 91 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 514 398 359 77 37 tfor:TF2744|3end_methionyl-tRNA_formyltransferase_2927378..2927528_POSITIVE 512 188 141 92 41 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 492 110 67 127 79 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 486 207 164 69 30 tfor:TF1816|3end_hypothetical_protein_1957974..1958124_POSITIVE 486 207 164 86 47 tfor:TF1817|3end_hypothetical_protein_1957991..1958141_POSITIVE 474 362 321 89 52 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 468 354 316 73 41 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 460 152 113 53 9 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE