Origin IGS: tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccttttcatttgaaccttgctaaataatgccttattctgctattataacctcacaccgcataagcttccgcctttgtttgaatatgttttccattagggagaaattctccataactcttctgatagttcgacaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaagtaaaattaaaatttaccggtatgacacttaacttcgattccgatgttttgcgtccgactgacatggttgctattgattccattttaatgattgtcgaactatcagaagagttatggagaatttctccctaatggaaaacatattcaaacaaaggcggaagcttatgcggtgtgaggttataatagcagaataaggcattatttagcaaggttcaaatgaaaagggatgttatagcaaggcaatacatatgatagagaaatccctaaaacttctttttctaattctaaaactgaacaatgagatagctattgtatttatcccggattccacaatagaagaaataagaatgaatttaattcaggccttcagccctccgaacaaggtctggatatcggtatcccacccttgcgcgggatggggctgaaataactagggcttttcagccctttcggacaatagtcaatggttaattgttatgcgtggaacacgccgaaccatgtatttggaaaataaccattaaccattgaaaaacagccctgaaagggcgagatca Mask Tandem Repeat Region ================================================ tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccttttcatttgaaccttgctaaataatgccttattctgctattataacctcacaccgcataagcttccgcctttgtttgaatatgttttccattagggagaaattctccataactcttctgatagttcgacaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Find is-nt database================================================ Query_seq: TF2025:TF2026|TF2025:TF2026:hypothetical protein:hypothetical protein:->->:2182241..2182798 558 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccttttcatttgaaccttgctaaataatgccttattctgctattataacctcacaccgcataagcttccgcctttgtttgaatatgttttccattagggagaaattctccataactcttctgatagttcgacaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2025:TF2026|TF2025:TF2026:hypothetical protein:hypothetical protein:->->:2182241..2182798 558 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccttttcatttgaaccttgctaaataatgccttattctgctattataacctcacaccgcataagcttccgcctttgtttgaatatgttttccattagggagaaattctccataactcttctgatagttcgacaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 19 Min: 332 Max: 460 Len: 129 Subject: gi|73670611|ref|YP_306626.1| hypothetical protein Mbar_A3162 [Methanosarcina barkeri str. fusaro] HSP 1 e-value: 2.0E-6 bit: 49.3 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F L P E N I H I Q S K A E T Y T V E G F N G I L R H F L A R L R R K .................................................................................................. Subject: gi|73667599|ref|YP_303614.1| hypothetical protein Mbar_A0043 [Methanosarcina barkeri str. fusaro] HSP 1 e-value: 2.0E-6 bit: 48.9 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 40 Subject End: 82 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F L P E N I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20092127|ref|NP_618202.1| hypothetical protein MA3312 [Methanosarcina acetivorans C2A] HSP 1 e-value: 3.0E-6 bit: 48.5 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E K Y T V E G Y N S I L R H F L A R L R R K .................................................................................................. Subject: gi|21228798|ref|NP_634720.1| hypothetical protein MM2696 [Methanosarcina mazei Go1] HSP 1 e-value: 3.0E-6 bit: 48.5 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 2 Subject End: 44 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W K P Y A E F I P E T I H T Q S K A E T Y T V E G Y N S I F R H F L A R L R R K .................................................................................................. Subject: gi|20090176|ref|NP_616251.1| hypothetical protein MA1314 [Methanosarcina acetivorans C2A] HSP 1 e-value: 3.0E-6 bit: 48.1 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E N I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20092643|ref|NP_618718.1| hypothetical protein MA3847 [Methanosarcina acetivorans C2A] HSP 1 e-value: 3.0E-6 bit: 48.1 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 64 Subject End: 106 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E N I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20092900|ref|NP_618975.1| hypothetical protein MA4107 [Methanosarcina acetivorans C2A] HSP 1 e-value: 3.0E-6 bit: 48.1 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E N I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|73668851|ref|YP_304866.1| hypothetical protein Mbar_A1324 [Methanosarcina barkeri str. fusaro] HSP 1 e-value: 4.0E-6 bit: 47.8 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F L P E N I H T Q S K A E T Y T V E G Y N G I L K H F L A R L R R K .................................................................................................. Subject: gi|73668855|ref|YP_304870.1| hypothetical protein Mbar_A1328 [Methanosarcina barkeri str. fusaro] HSP 1 e-value: 4.0E-6 bit: 47.8 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F L P E N I H T Q S K A E T Y T V E G Y N G I L K H F L A R L R R K .................................................................................................. Subject: gi|20090502|ref|NP_616577.1| hypothetical protein MA1649 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20091003|ref|NP_617078.1| hypothetical protein MA2161 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20091302|ref|NP_617377.1| hypothetical protein MA2471 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A A F L P E T I H T Q S K A E T Y T V E G Y N S I L R H F L A R L R R K .................................................................................................. Subject: gi|20091557|ref|NP_617632.1| hypothetical protein MA2733 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20091609|ref|NP_617684.1| hypothetical protein MA2786 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20091822|ref|NP_617897.1| hypothetical protein MA3004 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 3 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20092037|ref|NP_618112.1| hypothetical protein MA3221 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20092188|ref|NP_618263.1| hypothetical protein MA3374 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20093014|ref|NP_619089.1| hypothetical protein MA4224 [Methanosarcina acetivorans C2A] HSP 1 e-value: 6.0E-6 bit: 47.4 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E T I H T Q S K A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Subject: gi|20090730|ref|NP_616805.1| hypothetical protein MA1880 [Methanosarcina acetivorans C2A] HSP 1 e-value: 1.0E-5 bit: 46.6 Len: 129 Query Start:332 Query End:460 Subject Strand: null Subject Start: 42 Subject End: 84 ........................................................................................................................................................................................................................................................................................................................................... S N Y Q K S Y G E F L P N G K H I Q T K A E A Y A V * G Y N S R I R H Y L A R F K * K .................................................................................................. ........................................................................................................................................................................................................................................................................................................................................... T D H W R A Y A E F I P E N I H T Q S E A E T Y T V E G Y N G I L R H F L A R L R R K .................................................................................................. Find nr database================================================ Query_seq: TF2025:TF2026|TF2025:TF2026:hypothetical protein:hypothetical protein:->->:2182241..2182798 558 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 90 End: 185 ......................................................................................... M D Y C P K G L K S P S Y F S P I P R K G G I P I S R P C S E G ..................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 197 End: 322 .................................................................................................................................................................................................... M K F I L I S S I V E S G I N T I A I S L F S F R I R K R S F R D F S I I C I A L L ............................................................................................................................................................................................................................................ Protein_Len: 43 Strand: + Start: 353 End: 481 ................................................................................................................................................................................................................................................................................................................................................................ M M P Y S A I I T S H R I S F R L C L N M F S I R E K F S I T L L I V R Q S L K W N Q ............................................................................. Protein_Len: 42 Strand: + Start: 433 End: 558 ................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L H N S S D S S T I I K M E S I A T M S V G R K T S E S K L S V I P V N F N F T L Protein_Len: 36 Strand: - Start: 426 End: 533 ......................................................................................................................................................................................................................................................................................................................................................................................................................................... P S I R W L E E S L E V I M L I S D I A V M D T P R L V D S D F N L T M ......................... Protein_Len: 30 Strand: - Start: 364 End: 453 ........................................................................................................................................................................................................................................................................................................................................................................... E A I I V E C R M L K R R Q K F I N E M L S F N E M V R R M ......................................................................................................... Protein_Len: 60 Strand: - Start: 133 End: 312 .................................................................................................................................... G W G A C P H S V S I W V K N P P S F A Q I L N M R I E E I T S D P I F V I A I E N N L K L I L F L L K L S K E I M H M ...................................................................................................................................................................................................................................................... Protein_Len: 38 Strand: - Start: 128 End: 241 ............................................................................................................................... K L G M G R L P P I G I D L G Q E S P Q L G S N F E N K N R R N H F G P Y M ............................................................................................................................................................................................................................................................................................................................. tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2025:TF2026|TF2025:TF2026:hypothetical protein:hypothetical protein:->->:2182241..2182798 558 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Intra-Species Hit: Count: 7 Min: 1 Max: 558 Len: 558 Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 0.0 bit: 656.0 Len: 331 Query Start:1 Query End:331 Subject Strand: POSITIVE Subject Start: 1 Subject End: 331 tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatccc................................................................................................................................................................................................................................... tgatctcgccctttcagggctgtttttcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaatacaatagctatctcattgttcagttttagaattagaaaaagaagttttagggatttctctatcatatgtattgccttgctataacatccc................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-48 bit: 194.0 Len: 98 Query Start:461 Query End:558 Subject Strand: POSITIVE Subject Start: 461 Subject End: 558 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................caatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................caatcattaaaatggaatcaatagcaaccatgtcagtcggacgcaaaacatcggaatcgaagttaagtgtcataccggtaaattttaattttacttta Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 9.0E-48 bit: 192.0 Len: 233 Query Start:3 Query End:235 Subject Strand: NEGATIVE Subject Start: 132 Subject End: 361 ..atctcgccctttcagggctgtttttcaatggtta-------atggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccggg................................................................................................................................................................................................................................................................................................................................... ..atctcgccctttcagggctgtttttcaattgttaattgtcaatggttatttgccgggtacatgattcggagtgtaccgcgcataaccattaaccattg-------tccgaaagggctgaaa-gcccgaattatttcagccccatcccgc--aagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatccggg................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF3003_TF3004|hypothetical protein:phosphoribosylformylglycinamidine (FGAM) synthase|POSITIVE:POSITIVE|[3220690,3220958]|269 HSP 1 e-value: 5.0E-43 bit: 176.0 Len: 140 Query Start:85 Query End:224 Subject Strand: POSITIVE Subject Start: 22 Subject End: 158 ....................................................................................taaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.............................................................................................................................................................................................................................................................................................................................................. ....................................................................................taaccattaaccattgtccgaaagggctgaaa-gcccgaattatttcagccccatcccgc--aagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.............................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 8.0E-42 bit: 172.0 Len: 172 Query Start:53 Query End:224 Subject Strand: NEGATIVE Subject Start: 21 Subject End: 182 ....................................................tacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.............................................................................................................................................................................................................................................................................................................................................. ....................................................tacatgattcggcgtgtaccacgcataaccattaaccattg-------tccgaaagggctgaaa-gcccgaattatttcagccctatcccgc--aagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.............................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 5.0E-37 bit: 157.0 Len: 176 Query Start:27 Query End:202 Subject Strand: NEGATIVE Subject Start: 200 Subject End: 365 ..........................tcaatggttaatggttattttccaaatacatggttcggcgtgttccacgcataacaattaaccattgactattgtccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaa.................................................................................................................................................................................................................................................................................................................................................................... ..........................tcaatggttaatggttatttgccgggtacatgattcggcgtgtaccacgcataac-------cattgaccattgtccgaaagggctgaaa-gcccgaattatttcagccccatcccgc--aagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaa.................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 1.0E-31 bit: 139.0 Len: 126 Query Start:115 Query End:240 Subject Strand: POSITIVE Subject Start: 271 Subject End: 396 ..................................................................................................................aaagccctagttatttcagccccatcccgcgcaagggtggg--ataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgtggaatccgggataaa.............................................................................................................................................................................................................................................................................................................................. ..................................................................................................................aaagcccgaattatttcagccccatcccgc--aagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggttaaa.............................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 1.0E-25 bit: 119.0 Len: 123 Query Start:102 Query End:224 Subject Strand: POSITIVE Subject Start: 252 Subject End: 371 .....................................................................................................ccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.............................................................................................................................................................................................................................................................................................................................................. .....................................................................................................ccgaaagggctgaaa-gcccgaattatttcagccccatcccgt--aagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattg.............................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUCUCGCCCUUUCAGGGCUGUUUUUCAAUGGUUAAUGGUUAUUUUCCAAAUACAUGGUUCGGCGUGUUCCACGCAUAACAAUUAACCAUUGACUAUUGUCCGAAAGGGCUGAAAAGCCCUAGUUAUUUCAGCCCCAUCCCGCGCAAGGGUGGGAUACCGAUAUCCAGACCUUGUUCGGAGGGCUGAAGGCCUGAAUUAAAUUCAUUCUUAUUUCUUCUAUUGUGGAAUCCGGGAUAAAUACAAUAGCUAUCUCAUUGUUCAGUUUUAGAAUUAGAAAAAGAAGUUUUAGGGAUUUCUCUAUCAUAUGUAUUGCCUUGCUAUAACAUCCCUUUUCAUUUGAACCUUGCUAAAUAAUGCCUUAUUCUGCUAUUAUAACCUCACACCGCAUAAGCUUCCGCCUUUGUUUGAAUAUGUUUUCCAUUAGGGAGAAAUUCUCCAUAACUCUUCUGAUAGUUCGACAAUCAUUAAAAUGGAAUCAAUAGCAACCAUGUCAGUCGGACGCAAAACAUCGGAAUCGAAGUUAAGUGUCAUACCGGUAAAUUUUAAUUUUACUUUA ((((...((((((((.((((.((...((((((((((((.((((....(((......)))....(((((...))))).)))).))))))))))))..)).)))))))))))).((((..((((((...(((((((((....(((.((((((...(((((....)))))...)))))).))).)))))))))................(((((.(((((((((..(..((((..((((((...........))))))...))))..)..))))...))))))))))...)))))).))))...))))......(((((.((((((.............(((..(((...((...(((((((........)).)))))...........((....))....))....)))..))).....(((((((..((((.....))))..........((((.(.....).))))....)))))))...)))))).............(((((..(((.(((....))).)))..))))).....)))))................. (-148.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGUAAAAUUAAAAUUUACCGGUAUGACACUUAACUUCGAUUCCGAUGUUUUGCGUCCGACUGACAUGGUUGCUAUUGAUUCCAUUUUAAUGAUUGUCGAACUAUCAGAAGAGUUAUGGAGAAUUUCUCCCUAAUGGAAAACAUAUUCAAACAAAGGCGGAAGCUUAUGCGGUGUGAGGUUAUAAUAGCAGAAUAAGGCAUUAUUUAGCAAGGUUCAAAUGAAAAGGGAUGUUAUAGCAAGGCAAUACAUAUGAUAGAGAAAUCCCUAAAACUUCUUUUUCUAAUUCUAAAACUGAACAAUGAGAUAGCUAUUGUAUUUAUCCCGGAUUCCACAAUAGAAGAAAUAAGAAUGAAUUUAAUUCAGGCCUUCAGCCCUCCGAACAAGGUCUGGAUAUCGGUAUCCCACCCUUGCGCGGGAUGGGGCUGAAAUAACUAGGGCUUUUCAGCCCUUUCGGACAAUAGUCAAUGGUUAAUUGUUAUGCGUGGAACACGCCGAACCAUGUAUUUGGAAAAUAACCAUUAACCAUUGAAAAACAGCCCUGAAAGGGCGAGAUCA ((((((..((((((.....((((((..(((.(..(((.(((....))).)))..).)))......)).)))).....))))))..))))))..((((............(((((((..(((...((((((..(((.(((((............((.((((....)))).))(((..((((..(((((((((.((((...((........))...))))....(((((((((.(((.(((...(......)........((....)).))).))))))))))))..........................)))))))))))))..)))..)))))...))).))))))..((((.......)))).....((((((((((((..(((((...(((((....)))))...)))))..)))...)))))))))....)))..))))))).((((((((((.......((((((((((((.((((((((((...)))))....(((......)))...))))).)))))))))))).........))))))))))..)))). (-154.49) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 864 210 161 108 59 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 822 220 171 58 9 tfor:TF0820|5end_hypothetical_protein_879656..879886_POSITIVE 818 150 101 211 165 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 818 150 101 211 165 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 746 180 131 227 180 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 732 137 91 231 186 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 720 80 31 134 85 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 711 380 331 218 169 tfor:TF0208|5end_transposase_element_234336..234566_POSITIVE 658 110 61 212 170 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 613 224 181 230 187 tfor:TF0449|5end_transposase_467891..468121_POSITIVE 580 196 151 226 180 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 576 155 111 47 7 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 543 95 57 109 65 tfor:TF1846|5end_hypothetical_protein_1996726..1996956_POSITIVE 509 80 31 182 135 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 497 196 151 172 117 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 492 99 57 229 182 tfor:TF1850|5end_Na+/H+-exchanging_protein_1998542..1998772_POSITIVE 473 100 53 197 143 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 472 100 57 108 60 tfor:TF1842|5end_hypothetical_protein_1992571..1992801_POSITIVE 460 95 57 96 51 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 458 189 141 211 162 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 873 220 171 66 17 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 822 220 171 51 2 tfor:TF0819|3end_phosphoglycerate_mutase_879729..879879_POSITIVE 723 230 181 72 23 tfor:TF0029|3end_hypothetical_protein_25062..25212_POSITIVE 661 80 31 134 85 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 499 198 153 73 38 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 493 198 152 85 41 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE 493 198 152 78 34 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 469 206 165 89 52 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 468 80 33 139 94 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 455 196 152 48 5 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 445 43 3 90 52 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 442 196 153 53 19 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 437 196 153 74 34 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 433 205 166 151 107 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 416 196 154 146 93 tfor:TF2149|3end_deoxyribose-phosphate_aldolase_2323110..2323260_POSITIVE 416 43 3 128 90 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 402 103 62 46 1 tfor:TF2817|3end_hypothetical_protein_3007626..3007776_POSITIVE 398 196 153 81 43 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 398 196 153 41 3 tfor:TF0343|3end_hypothetical_protein_366923..367073_POSITIVE 397 196 161 37 4 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE