Origin IGS: ttttcttctttttatatggttaatacatgattataaattccaccgcagaccaccgtagattttccgcccatacacaggtccccagaccatggttgcatcgaaatcgtctctccacggatcggatgaggcaacgatcgggtgcggttgcgtgaagtcaaacaggttttccacccccacatacaacggacagcttcttaaaccgcttcgtgatctgcgcgccgacgagcgtataagcatcgtaggttttctcccacaatgggttttttgcatcgggaatcggtaacgtgccgggcccgttgaactgcgccgtcgcatcgaactgccaccggtaaacgggcgtccgatatccgagcgtaatcagtcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acatcattgaaataataatttgatatgtttttcaggcatatatgtactttctgtgcgctgatcacgagtagtgggatatatggcccaatttaccgtgaaaggtacggtgctttttgagggtgctgacaaagcggcgggtgcgacgttgcatctgttgcagtcgcgtacggtagcgactgattacgctcggatatcggacgcccgtttaccggtggcagttcgatgcgacggcgcagttcaacgggcccggcacgttaccgattcccgatgcaaaaaacccattgtgggagaaaacctacgatgcttatacgctcgtcggcgcgcagatcacgaagcggtttaagaagctgtccgttgtatgtgggggtggaaaacctgtttgacttcacgcaaccgcacccgatcgttgcctcatccgatccgtggagagacgatttcgatgcaaccatggtctggggacctgtgtatgggcggaaaatctacggtggtctgcggtggaatttataatcatgtattaaccatataaaaagaagaaaa Mask Tandem Repeat Region ================================================ ttttcttctttttatatggttaatacatgattataaattccaccgcagaccaccgtagattttccgcccatacacaggtccccagaccatggttgcatcgaaatcgtctctccacggatcggatgaggcaacgatcgggtgcggttgcgtgaagtcaaacaggttttccacccccacatacaacggacagcttcttaaaccgcttcgtgatctgcgcgccgacgagcgtataagcatcgtaggttttctcccacaatgggttttttgcatcgggaatcggtaacgtgccgggcccgttgaactgcgccgtcgcatcgaactgccaccggtaaacgggcgtccgatatccgagcgtaatcagtcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Find is-nt database================================================ Query_seq: TF1993:TF1994|TF1993:TF1994:hypothetical protein:acyl-CoA synthetase:->->:2144583..2145119 537 ttttcttctttttatatggttaatacatgattataaattccaccgcagaccaccgtagattttccgcccatacacaggtccccagaccatggttgcatcgaaatcgtctctccacggatcggatgaggcaacgatcgggtgcggttgcgtgaagtcaaacaggttttccacccccacatacaacggacagcttcttaaaccgcttcgtgatctgcgcgccgacgagcgtataagcatcgtaggttttctcccacaatgggttttttgcatcgggaatcggtaacgtgccgggcccgttgaactgcgccgtcgcatcgaactgccaccggtaaacgggcgtccgatatccgagcgtaatcagtcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1993:TF1994|TF1993:TF1994:hypothetical protein:acyl-CoA synthetase:->->:2144583..2145119 537 ttttcttctttttatatggttaatacatgattataaattccaccgcagaccaccgtagattttccgcccatacacaggtccccagaccatggttgcatcgaaatcgtctctccacggatcggatgaggcaacgatcgggtgcggttgcgtgaagtcaaacaggttttccacccccacatacaacggacagcttcttaaaccgcttcgtgatctgcgcgccgacgagcgtataagcatcgtaggttttctcccacaatgggttttttgcatcgggaatcggtaacgtgccgggcccgttgaactgcgccgtcgcatcgaactgccaccggtaaacgggcgtccgatatccgagcgtaatcagtcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1993:TF1994|TF1993:TF1994:hypothetical protein:acyl-CoA synthetase:->->:2144583..2145119 537 ttttcttctttttatatggttaatacatgattataaattccaccgcagaccaccgtagattttccgcccatacacaggtccccagaccatggttgcatcgaaatcgtctctccacggatcggatgaggcaacgatcgggtgcggttgcgtgaagtcaaacaggttttccacccccacatacaacggacagcttcttaaaccgcttcgtgatctgcgcgccgacgagcgtataagcatcgtaggttttctcccacaatgggttttttgcatcgggaatcggtaacgtgccgggcccgttgaactgcgccgtcgcatcgaactgccaccggtaaacgggcgtccgatatccgagcgtaatcagtcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 8 Min: 34 Max: 361 Len: 328 Subject: UniRef90_Q8A8S4 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron HSP 1 e-value: 1.0E-21 bit: 66.6 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 314 Subject End: 363 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. V Y I G G E N L T N F K Q K N P I I D A A N P W G G N F D S T M I W G P V H G A K A Y I G I R F N L .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-21 bit: 59.3 Len: 192 Query Start:170 Query End:361 Subject Strand: null Subject Start: 252 Subject End: 318 ......................................................................................................................................................................... L I T L G Y R T P V Y R W Q F D A T A Q F N G P G T L P I P D - - A K N P L - W E K T Y D A Y T L V G A Q I T K R F K K L S V V C G G ................................................................................................................................................................................ ......................................................................................................................................................................... L L T A S Y Q T P L G I W Q F D A T L Q L N G G G R M P S P Y E L A D G Q L S W E R R Y G S F E Q L S A Q I T R Y F R R W S V Y I G G ................................................................................................................................................................................ Subject: UniRef90_Q64UQ2 Cluster: Putative TonB-dependent outer membrane receptor protein; n=2; Bacteroides fragilis|Rep: Putative TonB-dependent outer membrane receptor protein - Bacteroides fragilis HSP 1 e-value: 2.0E-21 bit: 68.9 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 686 Subject End: 735 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. I Y I G G E N L T N F K Q K N P I I D A A D P W G D R F D S T M I W G P V H G A K G Y I G V R F N L .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-21 bit: 56.2 Len: 192 Query Start:170 Query End:361 Subject Strand: null Subject Start: 624 Subject End: 690 ......................................................................................................................................................................... L I T L G Y R T P V Y R W Q F D A T A Q F N G P G T L P I P D A - - - K N P L W E K T Y D A Y T L V G A Q I T K R F K K L S V V C G G ................................................................................................................................................................................ ......................................................................................................................................................................... L V T A S Y Q T P L G L W Q F D A T W Q M N G G G R M P N P Y T L A D G T S S W D A R Y K G F S Q L S A Q V T R Y F R R W S I Y I G G ................................................................................................................................................................................ Subject: UniRef90_A2U429 Cluster: Putative TonB-dependent outer membrane receptor protein; n=1; Polaribacter dokdonensis MED152|Rep: Putative TonB-dependent outer membrane receptor protein - Polaribacter dokdonensis MED152 HSP 1 e-value: 3.0E-11 bit: 58.2 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 697 Subject End: 746 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. V Y L G A E N L T N V Q Q N N P V L A S D D P F G A H F D T T I V Y A P I F G R S V Y T G L R F N I .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-11 bit: 32.3 Len: 138 Query Start:191 Query End:328 Subject Strand: null Subject Start: 646 Subject End: 693 .............................................................................................................................................................................................. R W Q F D A T A Q F N G P G T L P I P D A K N P L W E K T - - Y D A Y T L V G A Q I T K R F K K ................................................................................................................................................................................................................. .............................................................................................................................................................................................. Q W K F D V T F N N I G K Q R L P N T S T K P A Q Y Q L S D M V D A Y Q L L N A Q I T K V F S K ................................................................................................................................................................................................................. Subject: UniRef90_A4AU96 Cluster: Putative TonB-dependent outer membrane receptor protein; n=1; Flavobacteriales bacterium HTCC2170|Rep: Putative TonB-dependent outer membrane receptor protein - Flavobacteriales bacterium HTCC2170 HSP 1 e-value: 4.0E-10 bit: 57.8 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 686 Subject End: 735 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. V Y I G G E N L S N F T Q K N P V L S A N D P F G A N F D T S I V Y A P I M G R M F Y G G F R Y K L .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 4.0E-10 bit: 28.9 Len: 165 Query Start:164 Query End:328 Subject Strand: null Subject Start: 635 Subject End: 691 ................................................................................................................................................................... R W Q F D A T A Q F N G P G T L P - - I P D A K N P L W E K T Y D A Y T L V G A Q I T K R F K K L S V V C G G G K ................................................................................................................................................................................................................. ................................................................................................................................................................... Q W R F D Y T L H A L G E Q R L P N T L A N P E E Y R L A E Y S D P Y S L M N A Q I T K V F N E S F E V Y I G G E ................................................................................................................................................................................................................. Subject: UniRef90_A4BYB6 Cluster: Putative TonB-dependent outer membrane receptor protein; n=1; Polaribacter irgensii 23-P|Rep: Putative TonB-dependent outer membrane receptor protein - Polaribacter irgensii 23-P HSP 1 e-value: 3.0E-8 bit: 53.1 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 708 Subject End: 757 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. I Y F G G E N I T N V Q Q E N P I L A S E A P F G P N F D T T I V Y A P I F G R A I Y A G L R F K I .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-8 bit: 27.3 Len: 171 Query Start:158 Query End:328 Subject Strand: null Subject Start: 657 Subject End: 715 ............................................................................................................................................................. R W Q F D A T A Q F N G P G T L P I P D - A K N P L W E K T Y D - - - A Y T L V G A Q I T K R F K K L S V V C G G G K P V ................................................................................................................................................................................................................. ............................................................................................................................................................. Q W K I D L T - - F N N I G R Q R L P N T A S N P I A Y Q L A T H S K S Y S L L N S Q I T K V F S N T F E I Y F G G E N I ................................................................................................................................................................................................................. Subject: UniRef90_Q1XVN6 Cluster: TonB-dependent receptor precursor; n=1; Flavobacterium johnsoniae UW101|Rep: TonB-dependent receptor precursor - Flavobacterium johnsoniae UW101 HSP 1 e-value: 6.0E-8 bit: 46.2 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 618 Subject End: 667 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. V Y A G G E N I G N Y K Q Q K A I L G A N D P F G P N F D A S V A Y A P I F G Q M Y Y A G L R F K I .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 6.0E-8 bit: 33.1 Len: 171 Query Start:158 Query End:328 Subject Strand: null Subject Start: 567 Subject End: 625 ............................................................................................................................................................. R W Q F D A T A Q F N G P G T L P I P D A K N P L W E K T Y D - - - A Y T L V G A Q I T K R F K K L S V V C G G G K P V ................................................................................................................................................................................................................. ............................................................................................................................................................. Q W K F D Y T F N W S G K Q Q L P Y T - A S N P A E D Q F P D F S P S Y A V M N A Q V T R V F S P V F E V Y A G G E N I ................................................................................................................................................................................................................. Subject: UniRef90_A1ZRF1 Cluster: Putative TonB-dependent outer membrane receptor protein; n=1; Microscilla marina ATCC 23134|Rep: Putative TonB-dependent outer membrane receptor protein - Microscilla marina ATCC 23134 HSP 1 e-value: 1.0E-7 bit: 58.2 Len: 144 Query Start:40 Query End:183 Subject Strand: null Subject Start: 693 Subject End: 740 ....................................... L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W .................................................................................................................................................................................................................................................................................................................................................................. ....................................... L Y V G S E N L T N F R Q D T P I I D P S N P F G S Q F D A G L V W A P V L G R M V Y A G L R F .................................................................................................................................................................................................................................................................................................................................................................. Subject: UniRef90_Q1W0H5 Cluster: Putative TonB-dependent outer membrane receptor protein; n=1; Psychroflexus torquis ATCC 700755|Rep: Putative TonB-dependent outer membrane receptor protein - Psychroflexus torquis ATCC 700755 HSP 1 e-value: 3.0E-7 bit: 52.0 Len: 150 Query Start:34 Query End:183 Subject Strand: null Subject Start: 695 Subject End: 744 ................................. L Y V G V E N L F D F T Q P H P I V A S S D P W R D D F D A T M V W G P V Y G R K I Y G G L R W N L .................................................................................................................................................................................................................................................................................................................................................................. ................................. I Y L G G E N V T N V R Q P N P I L S A D N P F G S N F D S N F V Y G P I F G S L Y Y A G L R Y K I .................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-7 bit: 25.0 Len: 159 Query Start:170 Query End:328 Subject Strand: null Subject Start: 644 Subject End: 699 ......................................................................................................................................................................... R W Q F D A T A Q F N G P G T L P I P D A K N P L W E K T Y D A Y T L - - V G A Q I T K R F K - K L S V V C G G ................................................................................................................................................................................................................. ......................................................................................................................................................................... Q W K F D A T Y N W I S A Q R F P S T D L S T P Q F Q L D D F S P T I G T L N L Q V T K V F S P K F E I Y L G G ................................................................................................................................................................................................................. ttttcttctttttatatggttaatacatgattannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 27 End: 149 .......................... M I I N S T A D H R R F S A H T Q V P R P W L H R N R L S T D R M R Q R S G A V A .................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 59 End: 280 .......................................................... M F R P Y T G P Q T M V A S K S S L H G S D E A T I G C G C V K S N R F S T P T Y N G Q L L K P L R D L R A D E R I S I ................................................................................................................................................................................................................................................................. Protein_Len: 48 Strand: + Start: 302 End: 445 ............................................................................................................................................................................................................................................................................................................. M R R R I E L P P V N G R P I S E R N Q S L P Y A T A T D A T S H P P L C Q H P Q K A P Y L S R ............................................................................................ Protein_Len: 39 Strand: + Start: 357 End: 473 .................................................................................................................................................................................................................................................................................................................................................................... M S R Y R T R L Q Q M Q R R T R R F V S T L K K H R T F H G K L G H I S H Y S ................................................................ Protein_Len: 41 Strand: + Start: 409 End: 531 ........................................................................................................................................................................................................................................................................................................................................................................................................................ M S A P S K S T V P F T V N W A I Y P T T R D Q R T E S T Y M P E K H I K L L F Q ...... Protein_Len: 30 Strand: - Start: 424 End: 513 ....................................................................................................................................................................................................................................................................................................................................................................................................................................... F A G Y R E R Y I P G Y I G S S T I L A C F T C I H R F F M ........................ Protein_Len: 60 Strand: - Start: 198 End: 467 ..................................................................................................................................................................................................... R A R Q V A G D C R V A V P L R A D S I R A Y D T A V T R S Q L L H L T A G A A K D A G E F L V T G K V T F Q A M Y G M ...................................................................... Protein_Len: 60 Strand: - Start: 158 End: 361 ............................................................................................................................................................. V S L K K F R K T I Q A G V L T Y A D Y T K E W L P N K A D P I P L T G P G N F Q A T A D F Q W R Y V P T R Y G L T I M ................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 34 End: 345 ................................. E V G V Y L P C S R L G S R S R R A S S R I L M T P K R G C H T K Q M P F R Y R A P G T S S R R R M S S G G T F P R G M ................................................................................................................................................................................................ Protein_Len: 34 Strand: - Start: 23 End: 124 ...................... Y M I I F E V A S W R L N E A W V C T G L G H N C R F R R E V S R M ............................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 55 Strand: - Start: 15 End: 179 .............. I T L V H N Y I G G C V V T S K G G M C L D G S W P Q M S I T E G R I P H P L S R T R N R S T L C T K W G W M ...................................................................................................................................................................................................................................................................................................................................................................... ttttcttctttttatatggttaatacatgattannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttcttctttttatatggttaatacatgattannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 60 HP_score: -2.1 Tail_Score: -4.3739 Start: 219 End: 243 Full_Region: TTCGTGATCTGCGCG CCGACGAGC GTATAA GCATCGTAGG TTTTCTCCCACAATG ..........................................................................................................................................................................................................................CCGACGAGCGTATAAGCATCGTAGG...................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 90 HP_score: -6.8 Tail_Score: -5.08824 Start: 250 End: 260 Full_Region: CATCGTAGGTTTTCT CCCA CAA TGGG TTTTTTGCATCGGGA .........................................................................................................................................................................................................................................................CCCACAATGGG..................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: TF1993:TF1994|TF1993:TF1994:hypothetical protein:acyl-CoA synthetase:->->:2144583..2145119 537 ttttcttctttttatatggttaatacatgattannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt Intra-Species Hit: Count: 1 Min: 1 Max: 537 Len: 537 Subject: tfor_TF1993_TF1994|hypothetical protein:acyl-CoA synthetase|POSITIVE:POSITIVE|[2144583,2145119]|537 HSP 1 e-value: 6.0E-95 bit: 349.0 Len: 176 Query Start:362 Query End:537 Subject Strand: POSITIVE Subject Start: 362 Subject End: 537 .........................................................................................................................................................................................................................................................................................................................................................................tcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt .........................................................................................................................................................................................................................................................................................................................................................................tcgctaccgtacgcgactgcaacagatgcaacgtcgcacccgccgctttgtcagcaccctcaaaaagcaccgtacctttcacggtaaattgggccatatatcccactactcgtgatcagcgcacagaaagtacatatatgcctgaaaaacatatcaaattattatttcaatgatgt HSP 2 e-value: 1.0E-9 bit: 65.9 Len: 33 Query Start:1 Query End:33 Subject Strand: POSITIVE Subject Start: 1 Subject End: 33 ttttcttctttttatatggttaatacatgatta........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ttttcttctttttatatggttaatacatgatta........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCUUCUUUUUAUAUGGUUAAUACAUGAUUAUAAAUUCCACCGCAGACCACCGUAGAUUUUCCGCCCAUACACAGGUCCCCAGACCAUGGUUGCAUCGAAAUCGUCUCUCCACGGAUCGGAUGAGGCAACGAUCGGGUGCGGUUGCGUGAAGUCAAACAGGUUUUCCACCCCCACAUACAACGGACAGCUUCUUAAACCGCUUCGUGAUCUGCGCGCCGACGAGCGUAUAAGCAUCGUAGGUUUUCUCCCACAAUGGGUUUUUUGCAUCGGGAAUCGGUAACGUGCCGGGCCCGUUGAACUGCGCCGUCGCAUCGAACUGCCACCGGUAAACGGGCGUCCGAUAUCCGAGCGUAAUCAGUCGCUACCGUACGCGACUGCAACAGAUGCAACGUCGCACCCGCCGCUUUGUCAGCACCCUCAAAAAGCACCGUACCUUUCACGGUAAAUUGGGCCAUAUAUCCCACUACUCGUGAUCAGCGCACAGAAAGUACAUAUAUGCCUGAAAAACAUAUCAAAUUAUUAUUUCAAUGAUGU .........(((.(((((.......(((((..............(((((....((((((((....((((.......((((....))))...(((((.......((((((.((....))..)))))).)))))....))))((((((....(((((......((....))....((.........))...)))))...)))))).....)))))))).(((.((((((......)).)))).))).....(((.....)))...)))))...((((..((((.....))))(((((.((((..((((.(((.(((((.........(((.....)))(((..((.....))..)))...(((((((........))))))).....))))).))).))))......(((.....)))....)))).....(((((.......))))).....))))).....)))).....))))).((((.(((...............)))))))....))))).))).((((((....)))))). (-146.66) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUCAUUGAAAUAAUAAUUUGAUAUGUUUUUCAGGCAUAUAUGUACUUUCUGUGCGCUGAUCACGAGUAGUGGGAUAUAUGGCCCAAUUUACCGUGAAAGGUACGGUGCUUUUUGAGGGUGCUGACAAAGCGGCGGGUGCGACGUUGCAUCUGUUGCAGUCGCGUACGGUAGCGACUGAUUACGCUCGGAUAUCGGACGCCCGUUUACCGGUGGCAGUUCGAUGCGACGGCGCAGUUCAACGGGCCCGGCACGUUACCGAUUCCCGAUGCAAAAAACCCAUUGUGGGAGAAAACCUACGAUGCUUAUACGCUCGUCGGCGCGCAGAUCACGAAGCGGUUUAAGAAGCUGUCCGUUGUAUGUGGGGGUGGAAAACCUGUUUGACUUCACGCAACCGCACCCGAUCGUUGCCUCAUCCGAUCCGUGGAGAGACGAUUUCGAUGCAACCAUGGUCUGGGGACCUGUGUAUGGGCGGAAAAUCUACGGUGGUCUGCGGUGGAAUUUAUAAUCAUGUAUUAACCAUAUAAAAAGAAGAAAA ............(((((...(((((((..((((..((...(((((((..((((((((((((...(((((..(((((.....(((((....(((((((.....)))))))....(((((.((((.......((((((((((((....)))))))))))).(((((((.((.....(((((....(((.(((....(((....)))....)))))).)))))))))))))).))))..))))).)))))(((.......)))..)))))............(((((((((......))))))))).......)))))))))))))))))..(((..((((((.....)))))).))).)))))))(((((.(((......))).)))))..)).((((((..(.(((((.......((((.(((...((((.....)))).(((((.....((((....))))..))))))))))))......))))).)..))))))))))..))).))))..))))).................... (-177.72) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 533 310 267 68 25 tfor:TF2725|5end_outer_membrane_protein,_TonB_dependent_receptor_2899408..2899638_POSITIVE 512 328 286 53 2 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 493 350 304 56 13 tfor:TF0462|5end_possible_cobalamin_biosynthesis_precorrin-3_methylase_477600..477830_POSITIVE 489 370 322 61 9 tfor:TF0777|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_826098..826328_POSITIVE 483 340 292 202 147 tfor:TF0098|5end_permease_108103..108333_POSITIVE 481 314 271 110 61 tfor:TF1299|5end_conserved_hypothetical_protein_1380937..1381167_POSITIVE 471 242 201 50 13 tfor:TF1258|5end_hypothetical_protein_1338321..1338551_POSITIVE 467 150 103 66 11 tfor:TF2677|5end_aspartate_transaminase_2850809..2851039_POSITIVE 462 330 282 55 6 tfor:TF1460|5end_6-phosphogluconate_dehydrogenase_1549262..1549492_POSITIVE 461 350 302 198 150 tfor:TF0971|5end_conserved_hypothetical_protein_1021355..1021585_POSITIVE 455 350 303 48 4 tfor:TF0361|5end_hypothetical_protein_379458..379688_POSITIVE 450 317 278 65 22 tfor:TF2429|5end_ABC_transporter,_ATP-binding/permease_component,_bacteriocin/lantibiotic_exporter_2610453..2610683_POSITIVE 446 437 392 217 162 tfor:TF2770|5end_conserved_hypothetical_protein_2956308..2956538_POSITIVE 445 370 321 56 1 tfor:TF2354|5end_dihydropteroate_synthase_2518541..2518771_POSITIVE 445 350 303 76 30 tfor:TF2165|5end_hypothetical_protein_2341654..2341884_POSITIVE 445 350 303 229 183 tfor:TF0369|5end_hypothetical_protein_385933..386163_POSITIVE 443 339 294 228 193 tfor:TF1549|5end_conserved_hypothetical_protein_1653993..1654223_POSITIVE 441 330 284 71 12 tfor:TF2573|5end_50S_ribosomal_protein_L15_2743310..2743540_POSITIVE 441 149 102 175 125 tfor:TF1069|5end_glucokinase_regulatory_protein,_sugar_phosphate_isomerase_domain_1138884..1139114_POSITIVE 440 310 261 61 6 tfor:TF2423|5end_papain_family_cysteine_protease_2601608..2601838_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 489 370 322 56 4 tfor:TF0776|3end_ABC_transporter,_permease_component_826173..826323_POSITIVE 480 350 305 58 3 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 455 350 303 48 4 tfor:TF0360|3end_hypothetical_protein_379538..379688_POSITIVE 453 145 106 58 19 tfor:TF1298|3end_possible_membrane_protein_1381197..1381347_POSITIVE 450 317 278 57 14 tfor:TF2428|3end_conserved_hypothetical_protein_2610525..2610675_POSITIVE 445 350 303 50 4 tfor:TF2164|3end_hypothetical_protein_2341708..2341858_POSITIVE 445 350 303 50 4 tfor:TF0368|3end_hypothetical_protein_385834..385984_POSITIVE 443 339 294 142 107 tfor:TF1548|3end_thiophene_and_furan_oxidation_protein_1653987..1654137_POSITIVE 433 328 286 73 19 tfor:TF2006|3end_uracil_permease_2158642..2158792_POSITIVE 433 379 333 149 98 tfor:TF0419|3end_conserved_hypothetical_protein_435794..435944_POSITIVE 430 310 262 146 102 tfor:TF2024|3end_conserved_hypothetical_protein_2181979..2182129_POSITIVE 421 339 294 100 48 tfor:TF2015|3end_N6-adenine-specific_DNA_methylase_2173020..2173170_POSITIVE 420 339 291 108 66 tfor:TF2900|3end_conserved_hypothetical_protein_3090029..3090179_POSITIVE 414 317 280 47 6 tfor:TF2426|3end_conserved_hypothetical_protein;_possible_TonB_receptor_2605931..2606081_POSITIVE 410 220 173 53 3 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE 409 390 342 51 7 tfor:TF0593|3end_membrane-associated_phospholipid_phosphatase_627051..627201_POSITIVE 408 254 213 91 36 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 408 350 304 143 99 tfor:TF0535|3end_surface_antigen_BspA_561701..561851_POSITIVE 406 325 284 145 95 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 406 344 302 143 100 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE