Origin IGS: agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacggccgagctgaaaggtcttctcaatcctatatgccggatgatcggcaggaagcagtatgctttccctcctcctgacaagatgtaaaaaaaatagatatttcgtaccccttcctttcaccccatttctcaattttatttcatacctttgtgcgataattacgaacatataaataaagatttact Mask Tandem Repeat Region ================================================ agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Find is-nt database================================================ Query_seq: TF1974:TF1975|TF1974:TF1975:glyoxalase I related protein (lactoylglutathione lyase):uridylate kinase:->->:2121489..2121675 187 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1974:TF1975|TF1974:TF1975:glyoxalase I related protein (lactoylglutathione lyase):uridylate kinase:->->:2121489..2121675 187 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1974:TF1975|TF1974:TF1975:glyoxalase I related protein (lactoylglutathione lyase):uridylate kinase:->->:2121489..2121675 187 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 53 End: 154 .................................................... M E K W G E R K G Y E I S I F F T S C Q E E G K H T A S C R S S G I ................................. Protein_Len: 60 Strand: + Start: 6 End: 185 ..... M F I Y M F V I I A Q R Y E I K L R N G V K G R G T K Y L F F L H L V R R R E S I L L P A D H P A Y R I E K T F Q L G R .. Protein_Len: 33 Strand: + Start: 88 End: 186 ....................................................................................... M Y F F Y I L S G G G K A Y C F L P I I R H I G L R R P F S S A V . Protein_Len: 37 Strand: - Start: 17 End: 127 ................ I N T I I A C L Y S I F N L F P T F P L P V F Y R N K K C R T L L L S L M ............................................................ agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1974:TF1975|TF1974:TF1975:glyoxalase I related protein (lactoylglutathione lyase):uridylate kinase:->->:2121489..2121675 187 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Intra-Species Hit: Count: 1 Min: 1 Max: 187 Len: 187 Subject: tfor_TF1974_TF1975|glyoxalase I related protein (lactoylglutathione lyase):uridylate kinase|POSITIVE:POSITIVE|[2121489,2121675]|187 HSP 1 e-value: 1.0E-87 bit: 323.0 Len: 187 Query Start:1 Query End:187 Subject Strand: POSITIVE Subject Start: 1 Subject End: 187 agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctannnnnnnnacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca agtaaatctttatttatatgttcgtaattatcgcacaaaggtatgaaataaaattgagaaatggggtgaaaggaaggggtacgaaatatctattttttttacatcttgtcaggaggagggaaagcatactgcttcctgccgatcatccggcatataggattgagaagacctttcagctcggccgtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAAAUCUUUAUUUAUAUGUUCGUAAUUAUCGCACAAAGGUAUGAAAUAAAAUUGAGAAAUGGGGUGAAAGGAAGGGGUACGAAAUAUCUAUUUUUUUUACAUCUUGUCAGGAGGAGGGAAAGCAUACUGCUUCCUGCCGAUCAUCCGGCAUAUAGGAUUGAGAAGACCUUUCAGCUCGGCCGUCA ........(((((((((((.((.((.........)).)).))))).)))))).....((..((((.(((((((...(((((.....)))))..((((((((.(((((((.((((((.((.........)).))))))((((......))))..)))))))))))))))))))))).))))....)). (-43.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACGGCCGAGCUGAAAGGUCUUCUCAAUCCUAUAUGCCGGAUGAUCGGCAGGAAGCAGUAUGCUUUCCCUCCUCCUGACAAGAUGUAAAAAAAAUAGAUAUUUCGUACCCCUUCCUUUCACCCCAUUUCUCAAUUUUAUUUCAUACCUUUGUGCGAUAAUUACGAACAUAUAAAUAAAGAUUUACU ........(.(.(((((((((((.(((........(((((......)))))((((((.....))))))........))).)))).............((....)).........))))))).).).........((((((((.(((..((((((.......))))))..)))))))))))....... (-30.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 158 111 222 164 tfor:TF2165|5end_hypothetical_protein_2341654..2341884_POSITIVE 377 149 105 224 180 tfor:TF2079|5end_permease_2245585..2245815_POSITIVE 376 165 123 137 94 tfor:TF1742|5end_TPR_repeat-containing_protein_1873129..1873359_POSITIVE 374 150 101 58 12 tfor:TF2249|5end_transfer_region-related_protein,_TraC_2422988..2423218_POSITIVE 367 149 104 73 21 tfor:TF2042|5end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2201647..2201877_POSITIVE 366 157 112 229 169 tfor:TF0960|5end_beta-galactosidase_1008618..1008848_POSITIVE 364 157 111 115 62 tfor:TF1299|5end_conserved_hypothetical_protein_1380937..1381167_POSITIVE 359 180 131 206 157 tfor:TF1135|5end_conserved_hypothetical_protein_1210938..1211168_POSITIVE 355 150 111 65 6 tfor:TF1161|5end_large_conductance_mechanosensitive_channel_protein_1240931..1241161_POSITIVE 352 150 105 220 184 tfor:TF3147|5end_adenylate_kinase_3383049..3383279_POSITIVE 344 150 101 201 135 tfor:TF1651|5end_integrase_1761296..1761526_POSITIVE 339 150 101 59 5 tfor:TF1334|5end_hypothetical_protein_1415996..1416226_POSITIVE 338 154 112 180 136 tfor:TF1352|5end_polysaccharide_deacetylase-like_protein_1432006..1432236_POSITIVE 337 148 104 192 145 tfor:TF2444|5end_regulatory_protein_RecX_2620957..2621187_POSITIVE 336 177 136 37 1 tfor:TF1064|5end_ampG_permease_1134004..1134234_POSITIVE 334 152 115 183 136 tfor:TF2655|5end_DNA_topoisomerase_I_2824543..2824773_POSITIVE 332 160 114 218 183 tfor:TF0175|5end_hypothetical_protein_185585..185815_POSITIVE 330 178 140 215 177 tfor:TF2846|5end_cobyric_acid_synthase_3034896..3035126_POSITIVE 330 178 140 63 25 tfor:TF2847|5end_hypothetical_protein_3034744..3034974_POSITIVE 330 150 102 108 42 tfor:TF2189|5end_conserved_hypothetical_protein_2363455..2363685_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 390 151 112 53 2 tfor:TF2740|3end_conserved_hypothetical_protein_2921972..2922122_POSITIVE 374 150 101 58 12 tfor:TF2248|3end_transfer_region-related_protein,_TraB_2423068..2423218_POSITIVE 369 159 112 74 11 tfor:TF0959|3end_periplasmic_protease_1008540..1008690_POSITIVE 355 150 111 62 3 tfor:TF1160|3end_MutT/nudix_family_protein_1241008..1241158_POSITIVE 338 154 112 144 100 tfor:TF1351|3end_conserved_hypothetical_protein_1432050..1432200_POSITIVE 334 152 115 119 72 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 330 150 102 108 42 tfor:TF2188|3end_conserved_hypothetical_protein_2363535..2363685_POSITIVE 329 180 132 108 60 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 326 150 105 105 63 tfor:TF0679|3end_hypothetical_protein_721489..721639_POSITIVE 325 167 122 81 35 tfor:TF2931|3end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3135584..3135734_POSITIVE 321 150 106 61 8 tfor:TF1350|3end_possible_transcriptional_regulator_1430636..1430786_POSITIVE 320 170 129 53 12 tfor:TF1685|3end_hypothetical_protein_1800492..1800642_POSITIVE 319 149 103 127 62 tfor:TF0320|3end_dihydroorotase_347518..347668_POSITIVE 318 154 114 149 118 tfor:TF0174|3end_hypothetical_protein_185600..185750_POSITIVE 317 160 111 44 4 tfor:TF0424|3end_outer_membrane_receptor,_TonB-linked_443122..443272_POSITIVE 311 177 131 66 15 tfor:TF1915|3end_NAD-dependent_protein_deacetylase,_SIR2_family_2068489..2068639_POSITIVE 309 150 105 50 13 tfor:TF0960|3end_beta-galactosidase_1010506..1010656_POSITIVE 308 90 56 138 99 tfor:TF1893|3end_hypothetical_protein_2046823..2046973_POSITIVE 307 163 130 38 2 tfor:TF0908|3end_integrase_fragment_951024..951174_POSITIVE 303 165 124 47 5 tfor:TF0141|3end_exodeoxyribonuclease_VII,_large_subunit_149830..149980_POSITIVE