Origin IGS: taaaacgcataacaagaaagaaaatgggtaaaaaaagaaaagttccgggaattaatggatcttcttctgccgacatcgctttcatgttgttgctcttcttccttttgacgacctcgatggataccgatatgggattagcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attcctctttttgatatcaatttcttgatcctgttcctgttttcaggaggacggggaagccttcgcgctaatcccatatcggtatccatcgaggtcgtcaaaaggaagaagagcaacaacatgaaagcgatgtcggcagaagaagatccattaattcccggaacttttctttttttacccattttctttcttgttatgcgtttta Mask Tandem Repeat Region ================================================ taaaacgcataacaagaaagaaaatgggtaaaaaaagaaaagttccgggaattaatggatcttcttctgccgacatcgctttcatgttgttgctcttcttccttttgacgacctcgatggataccgatatgggattagcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Find is-nt database================================================ Query_seq: TF1965:TF1966|TF1965:TF1966:conserved hypothetical protein:conserved hypothetical protein:->->:2114924..2115128 205 taaaacgcataacaagaaagaaaatgggtaaaaaaagaaaagttccgggaattaatggatcttcttctgccgacatcgctttcatgttgttgctcttcttccttttgacgacctcgatggataccgatatgggattagcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1965:TF1966|TF1965:TF1966:conserved hypothetical protein:conserved hypothetical protein:->->:2114924..2115128 205 taaaacgcataacaagaaagaaaatgggtaaaaaaagaaaagttccgggaattaatggatcttcttctgccgacatcgctttcatgttgttgctcttcttccttttgacgacctcgatggataccgatatgggattagcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1965:TF1966|TF1965:TF1966:conserved hypothetical protein:conserved hypothetical protein:->->:2114924..2115128 205 taaaacgcataacaagaaagaaaatgggtaaaaaaagaaaagttccgggaattaatggatcttcttctgccgacatcgctttcatgttgttgctcttcttccttttgacgacctcgatggataccgatatgggattagcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 30 Max: 137 Len: 108 Subject: UniRef90_Q7MW65 Cluster: Hypothetical protein; n=1; Porphyromonas gingivalis|Rep: Hypothetical protein - Porphyromonas gingivalis (Bacteroides gingivalis) HSP 1 e-value: 8.0E-6 bit: 51.6 Len: 108 Query Start:30 Query End:137 Subject Strand: null Subject Start: 4 Subject End: 39 ............................. K K R K V P G I N G S S S A D I A F M X X X X X X X X X S M D T D M G L .................................................................... ............................. K K R K T P G I N G S S S A D I A F M L L I F F L I T T S M D T D M G L .................................................................... taaaacgcataacaagaaagaaaatgggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 59 End: 160 .......................................................... M F F C R H R F H V V A L L P F D D L D G Y R Y G I S A K A S P S S ............................................. Protein_Len: 60 Strand: + Start: 9 End: 203 ........ M T R K K M G K K R K V P G I N G S S S A D I A F M L L L F F L L T T S M D T D M G L A R R L P R P P E N R N R I K K L .. Protein_Len: 42 Strand: - Start: 3 End: 128 .. F A Y C S L F H T F F S F N R S N I S R R R G V D S E H Q Q E E E K Q R G R H I G M ............................................................................. Protein_Len: 60 Strand: - Start: 2 End: 205 . P L F L F T G P I L P D E E A S M A K M N N S K K R K V V E I S V S I P N A R L S G R G G S F L F L I L F N I D F L P M taaaacgcataacaagaaagaaaatgggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaacgcataacaagaaagaaaatgggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1965:TF1966|TF1965:TF1966:conserved hypothetical protein:conserved hypothetical protein:->->:2114924..2115128 205 taaaacgcataacaagaaagaaaatgggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat Intra-Species Hit: Count: 1 Min: 1 Max: 205 Len: 205 Subject: tfor_TF1965_TF1966|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2114924,2115128]|205 HSP 1 e-value: 3.0E-30 bit: 133.0 Len: 67 Query Start:139 Query End:205 Subject Strand: POSITIVE Subject Start: 139 Subject End: 205 ..........................................................................................................................................cgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat ..........................................................................................................................................cgcgaaggcttccccgtcctcctgaaaacaggaacaggatcaagaaattgatatcaaaaagaggaat HSP 2 e-value: 1.0E-7 bit: 58.0 Len: 29 Query Start:1 Query End:29 Subject Strand: POSITIVE Subject Start: 1 Subject End: 29 taaaacgcataacaagaaagaaaatgggt................................................................................................................................................................................ taaaacgcataacaagaaagaaaatgggt................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACGCAUAACAAGAAAGAAAAUGGGUAAAAAAAGAAAAGUUCCGGGAAUUAAUGGAUCUUCUUCUGCCGACAUCGCUUUCAUGUUGUUGCUCUUCUUCCUUUUGACGACCUCGAUGGAUACCGAUAUGGGAUUAGCGCGAAGGCUUCCCCGUCCUCCUGAAAACAGGAACAGGAUCAAGAAAUUGAUAUCAAAAAGAGGAAU ......((((((((.(((((...((((((......(((((((.((((........)))).))).)))))))..)))..))))).))))).)))..((((((.((((((...(((.(((((...((......))......((....))....))))).(((((....)))))..))).((((....))))..)))))).)))))). (-54.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCCUCUUUUUGAUAUCAAUUUCUUGAUCCUGUUCCUGUUUUCAGGAGGACGGGGAAGCCUUCGCGCUAAUCCCAUAUCGGUAUCCAUCGAGGUCGUCAAAAGGAAGAAGAGCAACAACAUGAAAGCGAUGUCGGCAGAAGAAGAUCCAUUAAUUCCCGGAACUUUUCUUUUUUUACCCAUUUUCUUUCUUGUUAUGCGUUUUA .(((.((((((((((.....(((((((.(((...(((((....)))))))))))))))..(((((.......((......)).......)))))..)))))))))).)))..(((..((((.(((((.((((..((.((((((((((((..........))).....))))))))).))))))..))))).)))).)))...... (-52.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 160 112 223 163 tfor:TF2695|5end_prephenate_dehydratase_2869618..2869848_POSITIVE 383 170 124 189 133 tfor:TF2985|5end_glycosyltransferase_protein_3200307..3200537_POSITIVE 381 109 61 188 132 tfor:TF0118|5end_5-keto-D-gluconate_5-reductase_129768..129998_POSITIVE 377 159 115 229 186 tfor:TF3015|5end_hypothetical_protein_3238430..3238660_POSITIVE 372 160 112 219 155 tfor:TF1155|5end_co-chaperonin,_groES_family;__heat_shock_protein_10_1236804..1237034_POSITIVE 360 154 111 189 139 tfor:TF2215|5end_conserved_hypothetical_protein_2387501..2387731_POSITIVE 358 170 124 132 79 tfor:TF1317|5end_hypothetical_protein_1391378..1391608_POSITIVE 357 157 113 206 152 tfor:TF1835|5end_possible_dihydroxy-acid_dehydratase_1984201..1984431_POSITIVE 350 160 113 215 169 tfor:TF1168|5end_hypothetical_protein_1248755..1248985_POSITIVE 348 108 61 86 18 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 345 119 71 218 181 tfor:TF2561|5end_50S_ribosomal_protein_L16_2738693..2738923_POSITIVE 338 159 111 63 3 tfor:TF1064|5end_ampG_permease_1134004..1134234_POSITIVE 337 110 65 159 118 tfor:TF1090|5end_hypothetical_protein_1159032..1159262_POSITIVE 337 156 113 82 41 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 337 157 111 57 2 tfor:TF0093|5end_outer_membrane_protein,_TonB_dependent_receptor_100497..100727_POSITIVE 336 170 125 210 164 tfor:TF2647|5end_transcriptional_regulator,_possible_arylsulfatase_regulator_2816796..2817026_POSITIVE 336 155 113 62 14 tfor:TF2341|5end_hypothetical_protein_2505735..2505965_POSITIVE 336 96 51 85 27 tfor:TF1853|5end_homoserine_O-succinyltransferase_2006470..2006700_POSITIVE 335 160 114 43 2 tfor:TF2724|5end_anti-sigma_factor_2898193..2898423_POSITIVE 335 170 124 141 97 tfor:TF2231|5end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_2400307..2400537_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 109 61 148 92 tfor:TF0117|3end_2-dehydro-3-deoxygluconokinase_129808..129958_POSITIVE 370 158 112 151 93 tfor:TF1154|3end_RNA_pseudouridylate_synthetase_1236822..1236972_POSITIVE 359 108 61 75 22 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 359 108 61 94 41 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 352 158 113 77 25 tfor:TF0038|3end_conserved_hypothetical_protein_41213..41363_POSITIVE 337 156 113 49 8 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 336 96 51 81 23 tfor:TF1852|3end_prtQ_protein_2006546..2006696_POSITIVE 335 170 124 148 104 tfor:TF2230|3end_CTn_excision_protein_2400394..2400544_POSITIVE 331 110 62 147 110 tfor:TF1292|3end_sensor_histidine_protein_kinase_1374554..1374704_POSITIVE 327 183 144 79 41 tfor:TF2685|3end_iron-regulated_ABC-type_transporter_membrane_component_2862725..2862875_POSITIVE 326 157 112 65 15 tfor:TF0466|3end_amidophosphoribosyltransferase_485972..486122_POSITIVE 325 159 112 78 25 tfor:TF2339|3end_hypothetical_protein_2505783..2505933_POSITIVE 323 89 44 59 7 tfor:TF2847|3end_hypothetical_protein_3034985..3035135_POSITIVE 323 157 112 56 13 tfor:TF1680|3end_peptide_chain_release_factor_3_1794969..1795119_POSITIVE 320 161 129 104 66 tfor:TF0001|3end_hypothetical_protein_158..308_POSITIVE 318 155 111 136 71 tfor:TF3057|3end_conserved_hypothetical_protein_3281238..3281388_POSITIVE 318 110 61 141 91 tfor:TF2548|3end_30S_ribosomal_protein_S12_2731704..2731854_POSITIVE 318 157 113 48 6 tfor:TF0939|3end_ISPg1_transposase,_IS1106-related_transposase_981115..981265_POSITIVE 317 147 107 132 86 tfor:TF0517|3end_TPR-repeat-containing_protein_541807..541957_POSITIVE 316 108 69 139 104 tfor:TF1842|3end_hypothetical_protein_1992800..1992950_POSITIVE