Origin IGS: taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaacagaataatgagatagtaatttattgcaaagacaggacagctttcctcggccgatcggaagaccgccccgtcatggagaagacggagcagcgcatattttgttacaactttttta Mask Tandem Repeat Region ================================================ taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Find is-nt database================================================ Query_seq: TF1915:TF1916|TF1915:TF1916:NAD-dependent protein deacetylase, SIR2 family:conserved hypothetical protein; possible ATPase:->->:2068550..2068669 120 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1915:TF1916|TF1915:TF1916:NAD-dependent protein deacetylase, SIR2 family:conserved hypothetical protein; possible ATPase:->->:2068550..2068669 120 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1915:TF1916|TF1915:TF1916:NAD-dependent protein deacetylase, SIR2 family:conserved hypothetical protein; possible ATPase:->->:2068550..2068669 120 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 26 End: 118 ......................... M L R L L H D G A V F R S A E E S C P V F A I N Y Y L I I L F .. Protein_Len: 30 Strand: - Start: 3 End: 92 .. F L Q L L I H A A G D E G H R P P R G I P R P F S D Q R Q M ............................ Protein_Len: 34 Strand: - Start: 2 End: 103 . F F N Y C F I R Q E T K E M V P R D E S R G L F A T R D K C Y I V M ................. Protein_Len: 31 Strand: - Start: 1 End: 93 L F T T V F Y A S S R R R W S P A T K R D A S S L Q G T K A M ........................... taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 55 HP_score: -4.4 Tail_Score: -3.6215 Start: 44 End: 85 Full_Region: CTCCGTCTTCTCCAT GACGGGGCGGTCTTCC GATCGGCCGA GGAAAGCTGTCCTGTC TTTGCAATAAATTAC ...........................................GACGGGGCGGTCTTCCGATCGGCCGAGGAAAGCTGTCCTGTC................................... Find igs database================================================ Query_seq: TF1915:TF1916|TF1915:TF1916:NAD-dependent protein deacetylase, SIR2 family:conserved hypothetical protein; possible ATPase:->->:2068550..2068669 120 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Intra-Species Hit: Count: 1 Min: 1 Max: 120 Len: 120 Subject: tfor_TF1915_TF1916|NAD-dependent protein deacetylase, SIR2 family:conserved hypothetical protein; possible ATPase|POSITIVE:POSITIVE|[2068550,2068669]|120 HSP 1 e-value: 3.0E-62 bit: 238.0 Len: 120 Query Start:1 Query End:120 Subject Strand: POSITIVE Subject Start: 1 Subject End: 120 taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta taaaaaagttgtaacaaaatatgcgctgctccgtcttctccatgacggggcggtcttccgatcggccgaggaaagctgtcctgtctttgcaataaattactatctcattattctgtttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAGUUGUAACAAAAUAUGCGCUGCUCCGUCUUCUCCAUGACGGGGCGGUCUUCCGAUCGGCCGAGGAAAGCUGUCCUGUCUUUGCAAUAAAUUACUAUCUCAUUAUUCUGUUUUA .......(((((((..........(((((((((((........))))))))))).........(((..((((......))))))).)))))))........................... (-29.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACAGAAUAAUGAGAUAGUAAUUUAUUGCAAAGACAGGACAGCUUUCCUCGGCCGAUCGGAAGACCGCCCCGUCAUGGAGAAGACGGAGCAGCGCAUAUUUUGUUACAACUUUUUUA ...(((((((((....(((((....)))))((......((((......)))).(((.(.((....))).)))(((((........)))))....))...)))))))))............ (-25.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 541 85 42 68 26 tfor:TF1925|5end_hypothetical_protein_2074458..2074688_POSITIVE 497 68 26 202 143 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 477 64 24 218 175 tfor:TF1471|5end_conserved_hypothetical_protein_1564608..1564838_POSITIVE 472 70 24 144 98 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 467 69 21 201 146 tfor:TF2371|5end_conserved_hypothetical_protein_2536974..2537204_POSITIVE 460 68 25 192 137 tfor:TF1067|5end_conserved_hypothetical_protein_1136867..1137097_POSITIVE 458 70 21 152 94 tfor:TF0431|5end_hypothetical_protein_452178..452408_POSITIVE 458 70 21 137 79 tfor:TF0430|5end_hypothetical_protein_452163..452393_POSITIVE 451 70 25 212 176 tfor:TF1064|5end_ampG_permease_1134004..1134234_POSITIVE 448 68 21 198 148 tfor:TF2252|5end_transfer_region-related_protein,_TraF_2424621..2424851_POSITIVE 448 69 21 78 11 tfor:TF1174|5end_ABC_transporter,_permease_component_1256584..1256814_POSITIVE 443 69 24 78 33 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 439 66 22 67 15 tfor:TF1834|5end_3-isopropylmalate_dehydrogenase_1983161..1983391_POSITIVE 438 70 24 224 150 tfor:TF2655|5end_DNA_topoisomerase_I_2824543..2824773_POSITIVE 435 70 24 103 39 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 433 81 46 162 132 tfor:TF2788|5end_hypothetical_protein_2972201..2972431_POSITIVE 432 84 46 213 168 tfor:TF1812|5end_tryptophan_synthase,_beta_chain_1952696..1952926_POSITIVE 427 68 22 130 76 tfor:TF2231|5end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_2400307..2400537_POSITIVE 427 68 21 69 23 tfor:TF2038|5end_hypothetical_protein_2198978..2199208_POSITIVE 427 78 32 64 18 tfor:TF1600|5end_uroporphyrinogen-III_synthase_1713964..1714194_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 530 85 44 47 6 tfor:TF1915|3end_NAD-dependent_protein_deacetylase,_SIR2_family_2068489..2068639_POSITIVE 472 70 24 144 98 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 460 68 25 103 48 tfor:TF1066|3end_hypothetical_protein_1136858..1137008_POSITIVE 458 70 21 121 63 tfor:TF0429|3end_ABC_transporter,_ATP-binding_protein_452227..452377_POSITIVE 441 69 25 65 3 tfor:TF1173|3end_ABC_transporter,_permease_component_1256651..1256801_POSITIVE 433 81 46 139 109 tfor:TF2787|3end_30S_ribosomal_protein_S1_2972258..2972408_POSITIVE 427 68 22 137 83 tfor:TF2230|3end_CTn_excision_protein_2400394..2400544_POSITIVE 427 78 32 74 28 tfor:TF1599|3end_ribonuclease_P_protein_component_1714054..1714204_POSITIVE 422 70 23 133 72 tfor:TF2188|3end_conserved_hypothetical_protein_2363535..2363685_POSITIVE 410 68 22 145 89 tfor:TF2253|3end_conjugative_transposon_protein,_TraG_2427531..2427681_POSITIVE 401 70 25 132 80 tfor:TF1668|3end_possible_glycosylhydrolase_1783615..1783765_POSITIVE 394 69 22 88 23 tfor:TF1612|3end_conserved_hypothetical_protein_1729866..1730016_POSITIVE 389 79 31 149 95 tfor:TF1944|3end_conserved_hypothetical_protein_2093309..2093459_POSITIVE 388 69 22 91 44 tfor:TF1609|3end_hypothetical_protein_1728620..1728770_POSITIVE 386 90 48 65 22 tfor:TF2406|3end_anti-sigma_factor_2581659..2581809_POSITIVE 383 70 23 44 1 tfor:TF2266|3end_transfer_region-related_protein,_TraQ_2434949..2435099_POSITIVE 381 63 25 65 27 tfor:TF1255|3end_conserved_hypothetical_protein_1338151..1338301_POSITIVE 380 68 22 72 19 tfor:TF2883|3end_peptidyl-prolyl_cis-trans_isomerase_3075129..3075279_POSITIVE 380 78 38 146 100 tfor:TF2878|3end_conserved_hypothetical_protein;_possible_CAAX_amino_terminal_protease_family_3068283..3068433_POSITIVE 380 68 23 88 43 tfor:TF0144|3end_hypothetical_protein_151483..151633_POSITIVE