Origin IGS: tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gacgattgcgttttaaatgagacataatgtttttcagacttcgaaggtacgtctttttgatcagaaatcacagaaaagtcccgagcaattcgttctgagcagcaagaagagcgattatcggaatgcgcgttgctccgtgacta Mask Tandem Repeat Region ================================================ tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Find is-nt database================================================ Query_seq: TF1914:TF1915|TF1914:TF1915:conserved hypothetical protein:NAD-dependent protein deacetylase, SIR2 family:->->:2067717..2067859 143 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1914:TF1915|TF1914:TF1915:conserved hypothetical protein:NAD-dependent protein deacetylase, SIR2 family:->->:2067717..2067859 143 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1914:TF1915|TF1914:TF1915:conserved hypothetical protein:NAD-dependent protein deacetylase, SIR2 family:->->:2067717..2067859 143 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: - Start: 2 End: 127 . T V S C R A N R Y D S K K S S L V F Q E P S K Q S K Q D F L R V K S T Q F V N H R M ................ tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1914:TF1915|TF1914:TF1915:conserved hypothetical protein:NAD-dependent protein deacetylase, SIR2 family:->->:2067717..2067859 143 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: tfor_TF1914_TF1915|conserved hypothetical protein:NAD-dependent protein deacetylase, SIR2 family|POSITIVE:POSITIVE|[2067717,2067859]|143 HSP 1 e-value: 8.0E-76 bit: 283.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc tagtcacggagcaacgcgcattccgataatcgctcttcttgctgctcagaacgaattgctcgggacttttctgtgatttctgatcaaaaagacgtaccttcgaagtctgaaaaacattatgtctcatttaaaacgcaatcgtc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUCACGGAGCAACGCGCAUUCCGAUAAUCGCUCUUCUUGCUGCUCAGAACGAAUUGCUCGGGACUUUUCUGUGAUUUCUGAUCAAAAAGACGUACCUUCGAAGUCUGAAAAACAUUAUGUCUCAUUUAAAACGCAAUCGUC .(((((((((((.....)).((((((((((((.(((...........))).)).)))).))))))....)))))))))...........(((((((..((((.....))))......)))))))................... (-24.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGAUUGCGUUUUAAAUGAGACAUAAUGUUUUUCAGACUUCGAAGGUACGUCUUUUUGAUCAGAAAUCACAGAAAAGUCCCGAGCAAUUCGUUCUGAGCAGCAAGAAGAGCGAUUAUCGGAAUGCGCGUUGCUCCGUGACUA ...((((.(...(((((.(((((.....(((.....)))..(....)...))))))))))...).)))).......(((((.(((((((.((((((((...((.......)).....))))))))...))))))).).)))). (-34.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 398 75 31 221 179 tfor:TF1185|5end_hypothetical_protein_1269238..1269468_POSITIVE 389 64 30 216 180 tfor:TF0543|5end_glycine_cleavage_system_p-protein_572251..572481_POSITIVE 385 64 30 59 23 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 385 64 30 214 178 tfor:TF1845|5end_hypothetical_protein_1996620..1996850_POSITIVE 383 47 3 64 8 tfor:TF1465|5end_modulator_of_DNA_gyrase_1554148..1554378_POSITIVE 356 64 30 124 88 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 354 76 31 63 8 tfor:TF2151|5end_uridine_kinase_2323033..2323263_POSITIVE 354 46 8 215 172 tfor:TF1475|5end_glucose-6-phosphate_1-dehydrogenase_1568034..1568264_POSITIVE 353 45 3 48 2 tfor:TF1684|5end_zinc_protease_1797396..1797626_POSITIVE 351 64 30 66 30 tfor:TF1841|5end_hypothetical_protein_1991936..1992166_POSITIVE 349 49 3 214 153 tfor:TF2381|5end_muramidase/hemagglutinin_2545757..2545987_POSITIVE 349 47 3 117 80 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 342 50 7 55 10 tfor:TF2846|5end_cobyric_acid_synthase_3034896..3035126_POSITIVE 335 47 7 176 135 tfor:TF0811|5end_conserved_hypothetical_protein;_posible_sugar_phosphate_isomerase_869787..870017_POSITIVE 333 44 5 168 123 tfor:TF2229.1|5end_rteR_regulatory_ortholog_(with_internal_stop)_2399454..2399684_POSITIVE 332 64 21 221 179 tfor:TF2822|5end_arginine_repressor_3011793..3012023_POSITIVE 329 95 52 215 178 tfor:TF1896|5end_conserved_hypothetical_protein;_possible_phage-related_protein_2047858..2048088_POSITIVE 328 62 23 186 145 tfor:TF2185|5end_outer_membrane_protein,_TonB_dependent_receptor_2357262..2357492_POSITIVE 327 50 2 216 164 tfor:TF2704|5end_ribonuclease_HII_2876242..2876472_POSITIVE 327 84 41 95 49 tfor:TF0312|5end_outer_membrane_protein_336473..336703_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 356 64 30 124 88 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 355 50 3 57 1 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 354 76 31 60 5 tfor:TF2149|3end_deoxyribose-phosphate_aldolase_2323110..2323260_POSITIVE 349 47 3 84 47 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 342 50 7 64 19 tfor:TF2847|3end_hypothetical_protein_3034985..3035135_POSITIVE 324 64 31 151 116 tfor:TF2737|3end_conserved_hypothetical_protein;_TPR-repeat-containing_protein_2919304..2919454_POSITIVE 324 86 41 90 42 tfor:TF2019|3end_dipeptidyl_peptidase_VI_2176401..2176551_POSITIVE 322 63 21 52 8 tfor:TF1934|3end_hypothetical_protein_2084079..2084229_POSITIVE 318 64 21 53 19 tfor:TF2494|3end_NAD-dependent_4-hydroxybutyrate_dehydrogenase_2669520..2669670_POSITIVE 316 47 6 59 19 tfor:TF1655|3end_long-chain-fatty-acid-CoA_ligase_1771068..1771218_POSITIVE 313 49 2 117 42 tfor:TF1302|3end_conserved_hypothetical_protein;_possible_tetracycline_resistance_element_1383836..1383986_POSITIVE 310 46 5 57 17 tfor:TF2563|3end_30S_ribosomal_protein_S17_2739679..2739829_POSITIVE 309 79 32 75 32 tfor:TF1160|3end_MutT/nudix_family_protein_1241008..1241158_POSITIVE 309 46 2 67 16 tfor:TF0858|3end_conserved_hypothetical_protein_911833..911983_POSITIVE 308 47 2 144 86 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 307 67 23 71 30 tfor:TF1930|3end_two-component_response_regulator_2078203..2078353_POSITIVE 307 70 24 81 30 tfor:TF0292|3end_conserved_hypothetical_protein_320640..320790_POSITIVE 305 44 2 141 105 tfor:TF2204|3end_conserved_hypothetical_protein_2379122..2379272_POSITIVE 298 48 2 55 7 tfor:TF2631|3end_conserved_hypothetical_protein_2802088..2802238_POSITIVE 297 45 6 109 66 tfor:TF0001|3end_hypothetical_protein_158..308_POSITIVE