Origin IGS: gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtatacgttgtttttaatgtttgatttttgagttacaaaagtaggctatttttcggaatatcaaaaacacatcagtgcaatgtatgaagacaataatgaatattaccgtttatgtatagggctgtcgagcgaactcatcgacctgcattaaagatatttacttcagaataacgtatacggatgatacaacttgtacatgccgatacacgtccgaaaaagagcgtaaactttaatcatttattcataaaaccaaccataataataaaagttaatcttttgtaataaaaaaactgtaacaaaattattttatttacgtttgctatcgtataataatcaaagaatttgaaatc Mask Tandem Repeat Region ================================================ gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Find is-nt database================================================ Query_seq: TF1896:TF1897|TF1896:TF1897:conserved hypothetical protein; possible phage-related protein:conserved hypothetical protein; possible aminopeptidase:->->:2049048..2049398 351 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1896:TF1897|TF1896:TF1897:conserved hypothetical protein; possible phage-related protein:conserved hypothetical protein; possible aminopeptidase:->->:2049048..2049398 351 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1896:TF1897|TF1896:TF1897:conserved hypothetical protein; possible phage-related protein:conserved hypothetical protein; possible aminopeptidase:->->:2049048..2049398 351 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 72 End: 176 ....................................................................... L L N V K I I I T P K I F L H N F N V S K K P R T D A H V L Q I M R M ............................................................................................................................................................................... gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1896:TF1897|TF1896:TF1897:conserved hypothetical protein; possible phage-related protein:conserved hypothetical protein; possible aminopeptidase:->->:2049048..2049398 351 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Intra-Species Hit: Count: 1 Min: 1 Max: 351 Len: 351 Subject: tfor_TF1896_TF1897|conserved hypothetical protein; possible phage-related protein:conserved hypothetical protein; possible aminopeptidase|POSITIVE:POSITIVE|[2049048,2049398]|351 HSP 1 e-value: 0.0 bit: 654.0 Len: 351 Query Start:1 Query End:351 Subject Strand: POSITIVE Subject Start: 1 Subject End: 351 gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagnnnnnnnattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact gatttcaaattctttgattattatacgatagcaaacgtaaataaaataattttgttacagtttttttattacaaaagattaacttttattattatggttggttttatgaataaatgattaaagtttacgctctttttcggacgtgtatcggcatgtacaagttgtatcatccgtatacgttattctgaagtaaatatctttaatgcaggtcgatgagttcgctcgacagccctatacataaacggtaatattcattattgtcttcatacattgcactgatgtgtttttgatattccgaaaaatagcctacttttgtaactcaaaaatcaaacattaaaaacaacgtatact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUUCAAAUUCUUUGAUUAUUAUACGAUAGCAAACGUAAAUAAAAUAAUUUUGUUACAGUUUUUUUAUUACAAAAGAUUAACUUUUAUUAUUAUGGUUGGUUUUAUGAAUAAAUGAUUAAAGUUUACGCUCUUUUUCGGACGUGUAUCGGCAUGUACAAGUUGUAUCAUCCGUAUACGUUAUUCUGAAGUAAAUAUCUUUAAUGCAGGUCGAUGAGUUCGCUCGACAGCCCUAUACAUAAACGGUAAUAUUCAUUAUUGUCUUCAUACAUUGCACUGAUGUGUUUUUGAUAUUCCGAAAAAUAGCCUACUUUUGUAACUCAAAAAUCAAACAUUAAAAACAACGUAUACU ............(((((((....((((........))))..............((((((.....((((((.((.((((((((((...........)))))))))).)))))))).....(((((....(((.(((((((((((((((.(((.((((((.....))).))))))))))))......(((((..............((..(((((((....)).))))).))...........((((((((....)))))))))))))..((..((((....))))...))....))))))))).)))..))))))))))).....))))))).................... (-68.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUACGUUGUUUUUAAUGUUUGAUUUUUGAGUUACAAAAGUAGGCUAUUUUUCGGAAUAUCAAAAACACAUCAGUGCAAUGUAUGAAGACAAUAAUGAAUAUUACCGUUUAUGUAUAGGGCUGUCGAGCGAACUCAUCGACCUGCAUUAAAGAUAUUUACUUCAGAAUAACGUAUACGGAUGAUACAACUUGUACAUGCCGAUACACGUCCGAAAAAGAGCGUAAACUUUAAUCAUUUAUUCAUAAAACCAACCAUAAUAAUAAAAGUUAAUCUUUUGUAAUAAAAAAACUGUAACAAAAUUAUUUUAUUUACGUUUGCUAUCGUAUAAUAAUCAAAGAAUUUGAAAUC ((((.((((.(((((.((((..(((((...(((((.......((.(((.(((((((((.((((.....(((....)))....(((((...((((...((..(((((((((.(((((.....((.(((((..(....).)))))..))......((........))......))))).)))).)))))))..)))).))))).))))....))))))))).))).))))))).)))))..))))...)))))...........(((((((.....)))))))................................)))).))))............((((.....)))).... (-64.91) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 245 203 145 103 tfor:TF1909|5end_cobalamin_(5`-phosphate)_synthase_2063207..2063437_POSITIVE 341 163 127 185 153 tfor:TF0676|5end_methyltransferase_719027..719257_POSITIVE 335 173 138 178 143 tfor:TF0243|5end_conserved_hypothetical_protein_273728..273958_POSITIVE 332 179 133 212 164 tfor:TF2790|5end_prismane_protein,_hybrid-cluster_protein_2973397..2973627_POSITIVE 326 239 195 59 7 tfor:TF1813|5end_peptide_methionine_sulfoxide_reductase_1954170..1954400_POSITIVE 317 186 147 101 65 tfor:TF1543|5end_conserved_hypothetical_protein_1649429..1649659_POSITIVE 314 168 122 75 39 tfor:TF2853|5end_2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_3040172..3040402_POSITIVE 311 186 143 231 188 tfor:TF2373|5end_UDP-3-O-acyl-GlcNAc_deacetylase_2538344..2538574_POSITIVE 303 179 137 40 9 tfor:TF1959|5end_conserved_hypothetical_protein_2109377..2109607_POSITIVE 303 173 135 163 120 tfor:TF1260|5end_chaperone_protein_DnaJ_1345325..1345555_POSITIVE 302 232 205 117 79 tfor:TF2296|5end_hypothetical_protein_2448892..2449122_POSITIVE 301 169 126 68 23 tfor:TF2677|5end_aspartate_transaminase_2850809..2851039_POSITIVE 301 170 122 221 166 tfor:TF1850|5end_Na+/H+-exchanging_protein_1998542..1998772_POSITIVE 296 180 133 227 186 tfor:TF3035|5end_aldose_1-epimerase_3260538..3260768_POSITIVE 296 180 131 187 139 tfor:TF2430|5end_hypothetical_protein_2612660..2612890_POSITIVE 293 244 204 157 120 tfor:TF1327|5end_L-fucose_permease_1405790..1406020_POSITIVE 292 247 206 149 106 tfor:TF0023|5end_possible_response_element_in_two_component_regulation_19446..19676_POSITIVE 291 166 122 58 11 tfor:TF2551|5end_30S_ribosomal_protein_S10_2734433..2734663_POSITIVE 291 237 197 227 188 tfor:TF0618|5end_shikimate_5-dehydrogenase_653203..653433_POSITIVE 290 175 135 229 176 tfor:TF0085|5end_ABC_transporter,_ATP-binding_protein_91517..91747_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 163 127 97 65 tfor:TF0675|3end_conserved_hypothetical_protein_719019..719169_POSITIVE 332 179 133 100 52 tfor:TF2789|3end_enoyl-[acyl-carrier-protein]_reductase_2973365..2973515_POSITIVE 317 186 147 94 58 tfor:TF1542|3end_transcriptional_regulator_1649502..1649652_POSITIVE 314 168 122 44 8 tfor:TF2852|3end_conserved_hypothetical_protein_3040221..3040371_POSITIVE 298 217 171 106 61 tfor:TF1730|3end_diaminopimelate_decarboxylase_1855907..1856057_POSITIVE 296 180 133 43 2 tfor:TF3034|3end_conserved_hypothetical_protein_3260434..3260584_POSITIVE 292 247 206 145 102 tfor:TF0022|3end_two-component_system_sensor_histidine_kinase_19522..19672_POSITIVE 289 168 124 138 92 tfor:TF2581|3end_50S_ribosomal_protein_L17_2749370..2749520_POSITIVE 289 155 123 119 79 tfor:TF1994|3end_acyl-CoA_synthetase_2147228..2147378_POSITIVE 286 231 204 134 92 tfor:TF1659|3end_hypothetical_protein_1775209..1775359_POSITIVE 286 190 143 65 16 tfor:TF1236|3end_L-rhamnose_isomerase_1322106..1322256_POSITIVE 286 231 204 134 92 tfor:TF1187|3end_hypothetical_protein_1269872..1270022_POSITIVE 286 231 204 77 35 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 286 231 204 150 108 tfor:TF0538|3end_hypothetical_protein_567135..567285_POSITIVE 283 245 207 120 73 tfor:TF2392|3end_alpha-galactosidase_2565506..2565656_POSITIVE 283 245 205 136 96 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 281 245 203 149 115 tfor:TF1908|3end_possible_phosphoglycerate_mutase_2063299..2063449_POSITIVE 279 231 204 116 74 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 278 164 137 31 3 tfor:TF0512|3end_2-oxoglutarate_oxidoreductase,_alpha_subunit_538264..538414_POSITIVE 276 246 205 57 5 tfor:TF2813|3end_possible_flavoprotein_A_3004540..3004690_POSITIVE