Origin IGS: taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatatgaatgttataaaattatcgccaatcgacaatttctattaccaattgacgataataattacccgtttaaatcttaaagtggatactcatgaaggcgtgctgcataaaatta Mask Tandem Repeat Region ================================================ taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Find is-nt database================================================ Query_seq: TF1862:TF1863|TF1862:TF1863:hypothetical protein:Fe-S oxidoreductase:->->:2018845..2018959 115 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1862:TF1863|TF1862:TF1863:hypothetical protein:Fe-S oxidoreductase:->->:2018845..2018959 115 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1862:TF1863|TF1862:TF1863:hypothetical protein:Fe-S oxidoreductase:->->:2018845..2018959 115 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 2 End: 112 . I K H L V G E H T D V K L N L R T I I I T L Q Y Y F N D I P S L K I V N M ... taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.0 Tail_Score: -4.43189 Start: 58 End: 97 Full_Region: TTAAACGGGTAATTA TTATCGTCAATTGGTAAT AGAA ATTGTCGATTGGCGATAA TTTTATAACATTCAT .........................................................TTATCGTCAATTGGTAATAGAAATTGTCGATTGGCGATAA.................. Find igs database================================================ Query_seq: TF1862:TF1863|TF1862:TF1863:hypothetical protein:Fe-S oxidoreductase:->->:2018845..2018959 115 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Intra-Species Hit: Count: 1 Min: 1 Max: 115 Len: 115 Subject: tfor_TF1862_TF1863|hypothetical protein:Fe-S oxidoreductase|POSITIVE:POSITIVE|[2018845,2018959]|115 HSP 1 e-value: 3.0E-59 bit: 228.0 Len: 115 Query Start:1 Query End:115 Subject Strand: POSITIVE Subject Start: 1 Subject End: 115 taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc taattttatgcagcacgccttcatgagtatccactttaagatttaaacgggtaattattatcgtcaattggtaatagaaattgtcgattggcgataattttataacattcatatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUAUGCAGCACGCCUUCAUGAGUAUCCACUUUAAGAUUUAAACGGGUAAUUAUUAUCGUCAAUUGGUAAUAGAAAUUGUCGAUUGGCGAUAAUUUUAUAACAUUCAUAUC .....(((((.((.....)).))))).(((((..((((.....)))).)))))...((((((((((((((..(((....)))..))))))))))))))................. (-23.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAUGAAUGUUAUAAAAUUAUCGCCAAUCGACAAUUUCUAUUACCAAUUGACGAUAAUAAUUACCCGUUUAAAUCUUAAAGUGGAUACUCAUGAAGGCGUGCUGCAUAAAAUUA (((.((((((.......(((((((.((((.(..(((....)))..).)))).))))))).......)))))).))).....(..(.(((.(......).))))..)......... (-16.84) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 87 48 191 148 tfor:TF1113|5end_possible_transposase_1183767..1183997_POSITIVE 253 95 60 169 134 tfor:TF1181|5end_methylated-DNA--[protein]-cysteine_S-methyltransferase_1267522..1267752_POSITIVE 248 85 48 76 41 tfor:TF1745|5end_hypothetical_protein_1879154..1879384_POSITIVE 247 49 8 231 175 tfor:TF2562|5end_50S_ribosomal_protein_L29_2739133..2739363_POSITIVE 245 86 46 172 134 tfor:TF0303|5end_conserved_hypothetical_protein_328331..328561_POSITIVE 241 52 11 63 23 tfor:TF2831|5end_ABC_transporter,_permease_component_3021317..3021547_POSITIVE 241 88 45 124 79 tfor:TF2707|5end_glutamine_amidotransferase_2878305..2878535_POSITIVE 232 56 13 226 185 tfor:TF2746|5end_hypothetical_protein_2927996..2928226_POSITIVE 232 34 9 98 73 tfor:TF0215|5end_tRNA/rRNA_methyltransferase,_TrmH_family_241463..241693_POSITIVE 231 35 9 205 176 tfor:TF2484|5end_hypothetical_protein_2660005..2660235_POSITIVE 231 99 60 203 152 tfor:TF2373|5end_UDP-3-O-acyl-GlcNAc_deacetylase_2538344..2538574_POSITIVE 231 40 3 134 99 tfor:TF2205|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2379055..2379285_POSITIVE 227 53 15 74 42 tfor:TF2345|5end_dGTP_triphosphohydrolase_2508372..2508602_POSITIVE 225 50 10 195 151 tfor:TF2402|5end_outer_membrane_protein,_possibly_involved_in_nutrient_binding_2574582..2574812_POSITIVE 223 86 46 63 20 tfor:TF1821|5end_cation_efflux_system_protein_1966501..1966731_POSITIVE 223 94 55 120 85 tfor:TF0336|5end_Xaa-Pro_aminopeptidase_360613..360843_POSITIVE 222 94 61 86 39 tfor:TF2674|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2848176..2848406_POSITIVE 221 93 60 68 35 tfor:TF0429|5end_ABC_transporter,_ATP-binding_protein_450177..450407_POSITIVE 217 50 3 229 183 tfor:TF3125|5end_recombination/repair_protein_3359808..3360038_POSITIVE 216 33 1 179 142 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 98 65 34 1 tfor:TF1862|3end_hypothetical_protein_2018784..2018934_POSITIVE 248 85 48 60 25 tfor:TF1744|3end_phenylacetate-CoA_ligase_1879218..1879368_POSITIVE 241 52 11 85 45 tfor:TF2830|3end_ABC_transporter,_ATP-binding_protein_3021419..3021569_POSITIVE 241 88 45 108 63 tfor:TF2706|3end_possible_aminopeptidase_2878369..2878519_POSITIVE 232 56 13 97 56 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 232 34 9 126 101 tfor:TF0214|3end_DNA_recombination_and_repair_protein,_RecN_241571..241721_POSITIVE 231 35 9 142 113 tfor:TF2483|3end_hypothetical_protein_2660022..2660172_POSITIVE 231 40 3 121 86 tfor:TF2204|3end_conserved_hypothetical_protein_2379122..2379272_POSITIVE 227 53 15 56 24 tfor:TF2344|3end_ATP/GTP_binding_protein_2508434..2508584_POSITIVE 223 94 55 138 103 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 222 94 61 102 55 tfor:TF2673|3end_conserved_hypothetical_protein_2848272..2848422_POSITIVE 215 50 6 145 89 tfor:TF2273|3end_conserved_hypothetical_protein_2436708..2436858_POSITIVE 212 65 26 58 22 tfor:TF1373|3end_hypothetical_protein_1450451..1450601_POSITIVE 210 50 10 140 94 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 210 50 8 46 3 tfor:TF0940|3end_transposase_982040..982190_POSITIVE 209 54 14 74 34 tfor:TF2225|3end_CTn_excision_protein_2395602..2395752_POSITIVE 208 93 53 132 86 tfor:TF1017|3end_30S_ribsomal_protein_S18_1074871..1075021_POSITIVE 206 35 8 131 109 tfor:TF1233|3end_dihydrodipicolinate_synthase_1318855..1319005_POSITIVE 206 96 66 83 48 tfor:TF0207|3end_conserved_hypothetical_protein_233505..233655_POSITIVE 202 110 64 145 99 tfor:TF1962|3end_TatD-related_deoxyribonuclease,_Mg-dependent_DNase_2113297..2113447_POSITIVE