Origin IGS: taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacctcgcaaaggaagacattccgggcgaaacaagaaaaaactttagccccgaaaatcttcctttgttgaaaaaaactttggtatggctatggaggtgtgccgaagattta Mask Tandem Repeat Region ================================================ taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Find is-nt database================================================ Query_seq: TF1813:TF1814|TF1813:TF1814:peptide methionine sulfoxide reductase:conserved hypothetical protein; possible biotin synthase related domain containing protein:->->:1955354..1955464 111 taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1813:TF1814|TF1813:TF1814:peptide methionine sulfoxide reductase:conserved hypothetical protein; possible biotin synthase related domain containing protein:->->:1955354..1955464 111 taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1813:TF1814|TF1813:TF1814:peptide methionine sulfoxide reductase:conserved hypothetical protein; possible biotin synthase related domain containing protein:->->:1955354..1955464 111 taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 1 End: 93 L D E A C R W L W V L T K K L L P L N E P S F N K R T E G P M .................. taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 55 HP_score: -2.6 Tail_Score: -4.15044 Start: 50 End: 100 Full_Region: tacctcgcaaa ggaagacattccgggcgaaa caagaaaaaac tttagccccgaaaatcttcc tttgttgaaaaaaac .................................................ggaagacattccgggcgaaacaagaaaaaactttagccccgaaaatcttcc........... Find igs database================================================ Query_seq: TF1813:TF1814|TF1813:TF1814:peptide methionine sulfoxide reductase:conserved hypothetical protein; possible biotin synthase related domain containing protein:->->:1955354..1955464 111 taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: tfor_TF1813_TF1814|peptide methionine sulfoxide reductase:conserved hypothetical protein; possible biotin synthase related domain containing protein|POSITIVE:POSITIVE|[1955354,1955464]|111 HSP 1 e-value: 2.0E-44 bit: 178.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 taaatcttcggcacacctccatagccataccaaagnnnnnnncaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta taaatcttcggcacacctccatagccataccaaagtttttttcaacaaaggaagattttcggggctaaagttttttcttgtttcgcccggaatgtcttcctttgcgaggta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUCUUCGGCACACCUCCAUAGCCAUACCAAAGUUUUUUUCAACAAAGGAAGAUUUUCGGGGCUAAAGUUUUUUCUUGUUUCGCCCGGAAUGUCUUCCUUUGCGAGGUA ...(((((.(((...........)))...................(((((((((((.(((.((((..(((......))).....)))).))).))))))))))).))))). (-33.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCUCGCAAAGGAAGACAUUCCGGGCGAAACAAGAAAAAACUUUAGCCCCGAAAAUCUUCCUUUGUUGAAAAAAACUUUGGUAUGGCUAUGGAGGUGUGCCGAAGAUUUA ....(((((((((((((..(((.((((.....(((......)))..)))).)))..)))))))))).)))......((((((((((.((....)).))))))))))..... (-39.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 325 100 51 143 99 tfor:TF1246|5end_protease,_with_chaperone_function_1329390..1329620_POSITIVE 313 106 62 63 17 tfor:TF2722|5end_conserved_hypothetical_protein_2896792..2897022_POSITIVE 313 109 61 221 165 tfor:TF1628|5end_conserved_hypothetical_protein_1740627..1740857_POSITIVE 301 109 61 218 167 tfor:TF0972|5end_conserved_hypothetical_protein_1022798..1023028_POSITIVE 300 82 48 200 166 tfor:TF3060|5end_conserved_hypothetical_protein_3283182..3283412_POSITIVE 299 100 54 182 130 tfor:TF2161|5end_conserved_hypothetical_protein_2336972..2337202_POSITIVE 293 107 62 51 1 tfor:TF1137|5end_asparaginyl-tRNA_synthetase_(asparagine--tRNA_ligase)_1212443..1212673_POSITIVE 292 96 54 154 109 tfor:TF0889|5end_conjugative_transposon_protein,_TraO_939423..939653_POSITIVE 291 110 72 209 157 tfor:TF1754|5end_conserved_hypothetical_protein_1890937..1891167_POSITIVE 290 89 42 183 131 tfor:TF1426|5end_N-acetylymuramoylalanyl-D-glutamate-2,6-diaminopi_melate_ligase_1509873..1510103_POSITIVE 288 104 61 208 165 tfor:TF2887|5end_phosphomannomutase/phosphoglucomutase_3076327..3076557_POSITIVE 288 95 53 72 24 tfor:TF1605|5end_outer_membrane_protein,_TonB_dependent_receptor_1718960..1719190_POSITIVE 287 110 61 72 17 tfor:TF1417|5end_conserved_hypothetical_protein_1499544..1499774_POSITIVE 285 109 65 132 104 tfor:TF2039|5end_possible_cell-cycle_regulation_histidine_triad_(HIT)_protein_2199318..2199548_POSITIVE 285 99 60 62 23 tfor:TF1856|5end_conserved_hypothetical_protein_2009969..2010199_POSITIVE 284 99 52 172 115 tfor:TF2011|5end_transposase_2163893..2164123_POSITIVE 283 96 56 222 173 tfor:TF1673|5end_ATP_synthase,_C_subunit_1787238..1787468_POSITIVE 279 46 4 99 54 tfor:TF2793|5end_possible_L-lysine_2,3-aminomutase_2977661..2977891_POSITIVE 279 109 71 174 128 tfor:TF2683|5end_translation_initiation_factor_IF-2_2857722..2857952_POSITIVE 279 106 70 190 156 tfor:TF0597|5end_hypothetical_protein_628788..629018_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 325 100 51 135 91 tfor:TF1245|3end_LemA_protein_1329462..1329612_POSITIVE 313 106 62 147 101 tfor:TF2721|3end_conserved_hypothetical_protein_2896956..2897106_POSITIVE 303 91 59 45 10 tfor:TF3053|3end_conserved_hypothetical_protein_3278889..3279039_POSITIVE 299 100 57 48 6 tfor:TF2659|3end_conserved_hypothetical_protein_2833055..2833205_POSITIVE 293 109 61 56 4 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 289 109 77 40 5 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 288 104 61 71 28 tfor:TF2886|3end_RNA_methylase_SpoU_family_3076270..3076420_POSITIVE 286 96 58 134 98 tfor:TF1738|3end_outer_membrane_protein_1866946..1867096_POSITIVE 279 109 71 142 96 tfor:TF2682|3end_N_utilization_substance_protein_A_2857770..2857920_POSITIVE 278 110 61 71 18 tfor:TF0095|3end_methyltransferase_106845..106995_POSITIVE 274 98 55 73 28 tfor:TF2406|3end_anti-sigma_factor_2581659..2581809_POSITIVE 272 100 51 100 51 tfor:TF2556|3end_conserved_hypothetical_protein_2737095..2737245_POSITIVE 272 110 62 88 40 tfor:TF1729|3end_aspartokinase_1854718..1854868_POSITIVE 270 87 50 63 24 tfor:TF2347|3end_outer_membrane_protein,_TonB_dependent_receptor_2511092..2511242_POSITIVE 269 109 64 102 46 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 266 109 68 95 55 tfor:TF2893|3end_conserved_hypothetical_protein;_possible_phosphoesterase_3082039..3082189_POSITIVE 266 98 51 119 62 tfor:TF1026|3end_beta-N-acetylglucosaminidase;_beta_lactamase_1085639..1085789_POSITIVE 266 95 55 94 54 tfor:TF0144|3end_hypothetical_protein_151483..151633_POSITIVE 264 109 61 119 66 tfor:TF2924|3end_DNA-binding_response_regulator/sensor_histidine_kinase_3125618..3125768_POSITIVE 264 108 61 136 87 tfor:TF2710|3end_ABC_transporter,_ATP-binding_protein/permease_component_2884615..2884765_POSITIVE