Origin IGS: gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgtctatcgtttgactttaaccattttatacaattaattattgccttgcaagtgacgggtttctcggtggtaaactccggtgtttggatgaaagcgatttctc Mask Tandem Repeat Region ================================================ gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Find is-nt database================================================ Query_seq: TF1806:TF1807|TF1806:TF1807:hypothetical protein:thioesterase family protein:->->:1948935..1949038 104 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1806:TF1807|TF1806:TF1807:hypothetical protein:thioesterase family protein:->->:1948935..1949038 104 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1806:TF1807|TF1806:TF1807:hypothetical protein:thioesterase family protein:->->:1948935..1949038 104 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 6 End: 104 ..... M A F I Q T P E F T T E K P V T C K A I I N C I K W L K S N D R H gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1806:TF1807|TF1806:TF1807:hypothetical protein:thioesterase family protein:->->:1948935..1949038 104 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Intra-Species Hit: Count: 1 Min: 1 Max: 104 Len: 104 Subject: tfor_TF1806_TF1807|hypothetical protein:thioesterase family protein|POSITIVE:POSITIVE|[1948935,1949038]|104 HSP 1 e-value: 1.0E-52 bit: 206.0 Len: 104 Query Start:1 Query End:104 Subject Strand: POSITIVE Subject Start: 1 Subject End: 104 gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat gagaaatcgctttcatccaaacaccggagtttaccaccgagaaacccgtcacttgcaaggcaataattaattgtataaaatggttaaagtcaaacgatagacat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAAAUCGCUUUCAUCCAAACACCGGAGUUUACCACCGAGAAACCCGUCACUUGCAAGGCAAUAAUUAAUUGUAUAAAAUGGUUAAAGUCAAACGAUAGACAU ........(((((....(((....(((.((((.........)))))))....))).))))).....(((((..(.....)..))))).(((........))).. (-13.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGUCUAUCGUUUGACUUUAACCAUUUUAUACAAUUAAUUAUUGCCUUGCAAGUGACGGGUUUCUCGGUGGUAAACUCCGGUGUUUGGAUGAAAGCGAUUUCUC ......(((((((....(((.(((....((((.........((((...))))....(((((((((....)).)))).))))))).))).))))))))))..... (-16.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 57 11 60 7 tfor:TF2184|5end_conserved_hypothetical_protein_2355578..2355808_POSITIVE 308 61 22 36 4 tfor:TF0214|5end_DNA_recombination_and_repair_protein,_RecN_239830..240060_POSITIVE 299 61 23 36 3 tfor:TF2422|5end_hypothetical_protein_2601298..2601528_POSITIVE 298 48 10 105 55 tfor:TF3160|5end_conserved_hypothetical_protein_3395531..3395761_POSITIVE 298 61 23 51 21 tfor:TF2276|5end_conserved_hypothetical_protein_2438226..2438456_POSITIVE 296 50 1 212 146 tfor:TF0890|5end_transfer_region-related_protein,_TraQ_940014..940244_POSITIVE 289 65 24 50 3 tfor:TF2832|5end_possible_proton/sodium:glutamate_symporter_protein_3022660..3022890_POSITIVE 283 61 25 48 19 tfor:TF2186|5end_outer_membrane_protein_2360291..2360521_POSITIVE 280 61 22 170 122 tfor:TF1042|5end_possible_surface_antigen_with_leucine-rich_repeat_1102957..1103187_POSITIVE 279 50 1 167 108 tfor:TF1777|5end_conserved_hypothetical_protein_1916653..1916883_POSITIVE 278 49 5 179 140 tfor:TF3018|5end_polyphosphate_kinase_3240122..3240352_POSITIVE 276 49 7 65 23 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 273 60 18 222 178 tfor:TF2949|5end_dihydroxynaphthoate_synthase_(naphthoate_synthase)_3156578..3156808_POSITIVE 271 65 28 214 170 tfor:TF2772|5end_tRNA_pseudouridine_synthase_B_2957383..2957613_POSITIVE 271 65 21 62 15 tfor:TF2606|5end_conserved_hypothetical_protein_2782260..2782490_POSITIVE 271 56 24 193 154 tfor:TF0195|5end_outer_membrane_protein_215631..215861_POSITIVE 270 56 11 187 138 tfor:TF2261|5end_transfer_region-related_protein,_TraM_2430496..2430726_POSITIVE 269 47 3 196 153 tfor:TF2906|5end_phosphatidylserine_decarboxylase_3102182..3102412_POSITIVE 268 61 21 228 187 tfor:TF2773|5end_S-adenosylmethionine_tRNA_ribosyltransferase_2958117..2958347_POSITIVE 267 59 23 231 177 tfor:TF2381|5end_muramidase/hemagglutinin_2545757..2545987_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 318 61 24 46 3 tfor:TF1109|3end_lipoprotein_1179997..1180147_POSITIVE 298 48 10 105 55 tfor:TF3159|3end_conserved_hypothetical_protein;_possible_transcriptional_regulator_3395611..3395761_POSITIVE 298 61 23 45 15 tfor:TF2275|3end_conserved_hypothetical_protein_2438300..2438450_POSITIVE 283 61 25 33 4 tfor:TF2185|3end_outer_membrane_protein,_TonB_dependent_receptor_2360356..2360506_POSITIVE 271 56 24 138 99 tfor:TF0194|3end_hypothetical_protein_215656..215806_POSITIVE 269 47 3 138 95 tfor:TF2904|3end_ATP-dependent__helicase,_possible_UvrD/REP_helicase_domain_protein_3102204..3102354_POSITIVE 264 61 24 55 18 tfor:TF2089|3end_acyl_carrier_protein_2259749..2259899_POSITIVE 263 49 10 130 85 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 260 61 25 128 95 tfor:TF0419|3end_conserved_hypothetical_protein_435794..435944_POSITIVE 259 56 16 36 2 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE 259 64 24 60 19 tfor:TF0939|3end_ISPg1_transposase,_IS1106-related_transposase_981115..981265_POSITIVE 259 61 25 44 11 tfor:TF0388|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_408846..408996_POSITIVE 257 63 24 60 17 tfor:TF1382|3end_hypothetical_protein_1457777..1457927_POSITIVE 257 63 24 56 13 tfor:TF1383|3end_hypothetical_protein_1457773..1457923_POSITIVE 256 61 21 37 6 tfor:TF2809|3end_conserved_hypothetical_protein;_possible_translation_factor_(SUA5)_3000511..3000661_POSITIVE 256 58 22 148 105 tfor:TF1041|3end_dihydroorotate_dehydrogenase,_catalytic_subunit_1103020..1103170_POSITIVE 255 67 24 48 2 tfor:TF2683|3end_translation_initiation_factor_IF-2_2860756..2860906_POSITIVE 254 36 6 79 36 tfor:TF0431|3end_hypothetical_protein_452470..452620_POSITIVE 254 36 6 112 69 tfor:TF0430|3end_hypothetical_protein_452503..452653_POSITIVE 252 56 25 91 55 tfor:TF2152|3end_uronate_isomerase_2328061..2328211_POSITIVE