Origin IGS: ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cctttacttcaaagtacaaaaataagatatttttttcgataaaatcattttgcgtatcaactcgaaccgatgtcggaagaaccgaacgaatccctttctcttatcctgctcgtcacgtttttacaggaagtaaagattttgttttccgcttctcattcgtctttttataccaacgattgaaaagtgcgaacaaaaaaattttttttcatgaacaatctctaatttctctttgttataaaaacacaaaccgttttttaggcacattgccacgaaacgaaaaaaagacggagagggaatgaaaaaagaaacagaaacagagagggaaataaacag Mask Tandem Repeat Region ================================================ ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Find is-nt database================================================ Query_seq: TF1798:TF1799|TF1798:TF1799:aldose 1-epimerase:quinolinate synthetase:->->:1940083..1940415 333 ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1798:TF1799|TF1798:TF1799:aldose 1-epimerase:quinolinate synthetase:->->:1940083..1940415 333 ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1798:TF1799|TF1798:TF1799:aldose 1-epimerase:quinolinate synthetase:->->:1940083..1940415 333 ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 1 End: 210 M F I S L S V S V S F F I P S P S F F R F V A M C L K N G L C F Y N K E K L E I V H E K K F F C S H F S I V G I K R R M ........................................................................................................................... Protein_Len: 30 Strand: + Start: 243 End: 332 .................................................................................................................................................................................................................................................. M R S V L P T S V R V D T Q N D F I E K N I L F L Y F E V K . Protein_Len: 34 Strand: - Start: 204 End: 305 ........................................................................................................................................................................................................... K R Y F R S S C S L L F P N T R N K R C R N S N I R L I I K D F F M ............................ Protein_Len: 36 Strand: - Start: 157 End: 264 ............................................................................................................................................................ D N T Y F S S H S A S F L I K V E Q L F T V L L I L S L S E N P E E S M ..................................................................... Protein_Len: 60 Strand: - Start: 38 End: 307 ..................................... F F I K K N A S K L R Q Y L F V F S F R F V F D K S G T F V H R A P Y S F P I R E T R G V D T R T S V C F S K I S F F M .......................... ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1798:TF1799|TF1798:TF1799:aldose 1-epimerase:quinolinate synthetase:->->:1940083..1940415 333 ctgtttatttccctctctgtttctgtttcttttttcattccctctccgtctttttttcgtttcgtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatgaaaaaaaatttttttgttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg Intra-Species Hit: Count: 1 Min: 64 Max: 333 Len: 270 Subject: tfor_TF1798_TF1799|aldose 1-epimerase:quinolinate synthetase|POSITIVE:POSITIVE|[1940083,1940415]|333 HSP 1 e-value: 1.0E-91 bit: 337.0 Len: 191 Query Start:143 Query End:333 Subject Strand: POSITIVE Subject Start: 143 Subject End: 333 ..............................................................................................................................................gttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgnnnnnnntatcttatttttgtactttgaagtaaagg ..............................................................................................................................................gttcgcacttttcaatcgttggtataaaaagacgaatgagaagcggaaaacaaaatctttacttcctgtaaaaacgtgacgagcaggataagagaaagggattcgttcggttcttccgacatcggttcgagttgatacgcaaaatgattttatcgaaaaaaatatcttatttttgtactttgaagtaaagg HSP 2 e-value: 3.0E-28 bit: 127.0 Len: 64 Query Start:64 Query End:127 Subject Strand: POSITIVE Subject Start: 64 Subject End: 127 ...............................................................gtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatg.............................................................................................................................................................................................................. ...............................................................gtggcaatgtgcctaaaaaacggtttgtgtttttataacaaagagaaattagagattgttcatg.............................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGGCAAUGUGCCUAAAAAACGGUUUGUGUUUUUAUAACAAAGAGAAAUUAGAGAUUGUUCAUGAAAAAAAAUUUUUUUGUUCGCACUUUUCAAUCGUUGGUAUAAAAAGACGAAUGAGAAGCGGAAAACAAAAUCUUUACUUCCUGUAAAAACGUGACGAGCAGGAUAAGAGAAAGGGAUUCGUUCGGUUCUUCCGACAUCGGUUCGAGUUGAUACGCAAAAUGAUUUUAUCGAAAAAAAUAUCUUAUUUUUGUACUUUGAAGUAAAGG ..(((.....)))..(((((((.....)))))))....(((((((((((.(((.(((.(((((((((........(((((((..(.(((((((.(((((..(....)..))))).)))))))..)..)))))))(((((...((((((.............))))))..)))))........(((((..(((..(((....)))...)))..)).)))........)))))).)))...))).))).))))))...)))))......... (-54.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCUUUACUUCAAAGUACAAAAAUAAGAUAUUUUUUUCGAUAAAAUCAUUUUGCGUAUCAACUCGAACCGAUGUCGGAAGAACCGAACGAAUCCCUUUCUCUUAUCCUGCUCGUCACGUUUUUACAGGAAGUAAAGAUUUUGUUUUCCGCUUCUCAUUCGUCUUUUUAUACCAACGAUUGAAAAGUGCGAACAAAAAAAUUUUUUUUCAUGAACAAUCUCUAAUUUCUCUUUGUUAUAAAAACACAAACCGUUUUUUAGGCACAUUGCCAC .(((((((((...(((..(((((..(((..((((((((((...(((..((((.((....)).))))..)))))))))))))......(((.....))).............)))..))))))))..))))))))).((((((((..(((((.(((.((((............)))).))).))))).))))))))...............(((((..............)))))..((((((.......))))))..(((.....))).. (-48.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 393 220 173 166 109 tfor:TF1446|5end_hypothetical_protein_1530649..1530879_POSITIVE 369 200 154 166 112 tfor:TF0017|5end_hypothetical_protein_10504..10734_POSITIVE 353 200 154 164 116 tfor:TF1185|5end_hypothetical_protein_1269238..1269468_POSITIVE 353 200 154 74 26 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 350 211 178 155 115 tfor:TF2469|5end_oxidoreductase,_possible_Gfo/Idh/MocA_family_2646505..2646735_POSITIVE 350 202 164 114 74 tfor:TF2230|5end_CTn_excision_protein_2399799..2400029_POSITIVE 350 219 171 146 94 tfor:TF0889|5end_conjugative_transposon_protein,_TraO_939423..939653_POSITIVE 346 208 163 142 89 tfor:TF2788|5end_hypothetical_protein_2972201..2972431_POSITIVE 344 200 154 92 41 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 342 210 165 220 176 tfor:TF1522|5end_hypothetical_protein_1629531..1629761_POSITIVE 341 213 178 71 30 tfor:TF0816|5end_hypothetical_protein_874217..874447_POSITIVE 336 198 154 51 7 tfor:TF1881|5end_hypothetical_protein_2033412..2033642_POSITIVE 336 209 161 69 31 tfor:TF0141|5end_exodeoxyribonuclease_VII,_large_subunit_148422..148652_POSITIVE 333 190 142 65 21 tfor:TF2527|5end_RNA_polymerase_ECF-type_sigma_factor_2710097..2710327_POSITIVE 332 219 177 173 127 tfor:TF1898|5end_RNA_polymerase_ECF-type_sigma_factor_2052115..2052345_POSITIVE 332 197 155 54 8 tfor:TF0431|5end_hypothetical_protein_452178..452408_POSITIVE 329 230 192 224 178 tfor:TF2044|5end_excinuclease_ABC,_subunit_A_2203228..2203458_POSITIVE 328 218 181 166 135 tfor:TF2473|5end_poly(A)_polymerase/tRNA_nucleotidyltransferase_2650470..2650700_POSITIVE 327 218 182 179 138 tfor:TF1161|5end_large_conductance_mechanosensitive_channel_protein_1240931..1241161_POSITIVE 327 218 180 211 176 tfor:TF0381|5end_cell_cycle_protein_401632..401862_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 200 154 113 65 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 353 200 154 147 99 tfor:TF1845|3end_hypothetical_protein_1997005..1997155_POSITIVE 353 200 154 131 83 tfor:TF1659|3end_hypothetical_protein_1775209..1775359_POSITIVE 350 219 171 149 97 tfor:TF0888|3end_transfer_region-related_protein,_TraN_939506..939656_POSITIVE 346 208 163 119 66 tfor:TF2787|3end_30S_ribosomal_protein_S1_2972258..2972408_POSITIVE 338 195 154 150 109 tfor:TF0016|3end_hypothetical_protein_10581..10731_POSITIVE 336 209 161 50 12 tfor:TF0140|3end_exodeoxyribonuclease_VII,_small_subunit_148483..148633_POSITIVE 334 210 178 151 112 tfor:TF2468|3end_hypothetical_protein_2646582..2646732_POSITIVE 332 219 177 85 39 tfor:TF1897|3end_conserved_hypothetical_protein;_possible_aminopeptidase_2052107..2052257_POSITIVE 328 218 181 84 53 tfor:TF2472|3end_GAF_domain-containing_protein_2650468..2650618_POSITIVE 327 218 180 132 97 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE 326 213 173 146 98 tfor:TF1445|3end_RNA_polymerase_sigma_factor_1530718..1530868_POSITIVE 323 207 176 42 5 tfor:TF1542|3end_transcriptional_regulator_1649502..1649652_POSITIVE 316 219 178 151 101 tfor:TF2917|3end_conserved_hypothetical_protein_3114161..3114311_POSITIVE 313 200 154 75 31 tfor:TF1583|3end_conserved_hypothetical_protein;_possible_endonuclease_1690223..1690373_POSITIVE 312 218 176 123 80 tfor:TF1530|3end_1-deoxyxylulose-5-phosphate_synthase_1636614..1636764_POSITIVE 309 208 164 134 79 tfor:TF0581|3end_single_stranded_DNA-binding_protein_611932..612082_POSITIVE 308 207 162 72 28 tfor:TF0178|3end_ferritin_188223..188373_POSITIVE 307 206 161 129 82 tfor:TF0986|3end_50S_ribosomal_protein_L31_1041757..1041907_POSITIVE 304 218 172 138 85 tfor:TF2518|3end_ATP-dependent_DNA_helicase_2697711..2697861_POSITIVE