Origin IGS: taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgtatacctctctgtttggatgtatcgtaccgaacaccttgtgcggagtatgaaagtattcggtcggattgtatgccgctgagagactctgctttattgaaatgataaaattttactttcgaggtcaaacgttttgtcttttggatgaaatgagctataaacagggtgtctctgattatttatctctta Mask Tandem Repeat Region ================================================ taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Find is-nt database================================================ Query_seq: TF1787:TF1788|TF1787:TF1788:conserved hypothetical protein:conserved hypothetical protein:->->:1927366..1927554 189 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1787:TF1788|TF1787:TF1788:conserved hypothetical protein:conserved hypothetical protein:->->:1927366..1927554 189 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1787:TF1788|TF1787:TF1788:conserved hypothetical protein:conserved hypothetical protein:->->:1927366..1927554 189 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 11 End: 187 .......... M I R D T L F I A H F I Q K T K R L T S K V K F Y H F N K A E S L S G I Q S D R I L S Y S A Q G V R Y D T S K Q R G I .. Protein_Len: 35 Strand: + Start: 84 End: 188 ................................................................................... M I S I K Q S L S A A Y N P T E Y F H T P H K V F G T I H P N R E V Y . Protein_Len: 33 Strand: - Start: 88 End: 186 ....................................................................................... K L L A S D R L P M C D S R I S E Y E A C P T R Y S V D L C L P M ... Protein_Len: 40 Strand: - Start: 2 End: 121 . L S L Y D S V G Q K Y S M E D L L C F T Q G R F Y F K I M E I F C L R E A A Y M .................................................................... taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1787:TF1788|TF1787:TF1788:conserved hypothetical protein:conserved hypothetical protein:->->:1927366..1927554 189 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Intra-Species Hit: Count: 1 Min: 1 Max: 189 Len: 189 Subject: tfor_TF1787_TF1788|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1927366,1927554]|189 HSP 1 e-value: 1.0E-103 bit: 375.0 Len: 189 Query Start:1 Query End:189 Subject Strand: POSITIVE Subject Start: 1 Subject End: 189 taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg taagagataaataatcagagacaccctgtttatagctcatttcatccaaaagacaaaacgtttgacctcgaaagtaaaattttatcatttcaataaagcagagtctctcagcggcatacaatccgaccgaatactttcatactccgcacaaggtgttcggtacgatacatccaaacagagaggtatacg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAGAUAAAUAAUCAGAGACACCCUGUUUAUAGCUCAUUUCAUCCAAAAGACAAAACGUUUGACCUCGAAAGUAAAAUUUUAUCAUUUCAAUAAAGCAGAGUCUCUCAGCGGCAUACAAUCCGACCGAAUACUUUCAUACUCCGCACAAGGUGUUCGGUACGAUACAUCCAAACAGAGAGGUAUACG ...(((....((((.(((.......))).))))..)))....................(((...(((((............................((.(((...))).))...........((((((((((((((............)))))))))))).))...............)))))..))) (-34.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUAUACCUCUCUGUUUGGAUGUAUCGUACCGAACACCUUGUGCGGAGUAUGAAAGUAUUCGGUCGGAUUGUAUGCCGCUGAGAGACUCUGCUUUAUUGAAAUGAUAAAAUUUUACUUUCGAGGUCAAACGUUUUGUCUUUUGGAUGAAAUGAGCUAUAAACAGGGUGUCUCUGAUUAUUUAUCUCUUA .((((((.(((.((((((((((...))).)))))))......((.((((((....)))))).)).)))..))))))....((((.((((((.(((((((((((......))))))..............(((((..((.....))..)))))....))))))))))).))))................. (-45.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 365 159 111 98 49 tfor:TF0146|5end_glutamine-dependent_NAD(+)_synthetase_154855..155085_POSITIVE 355 129 98 189 160 tfor:TF2831|5end_ABC_transporter,_permease_component_3021317..3021547_POSITIVE 326 172 134 193 151 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 326 172 134 72 30 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 321 172 134 213 169 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 312 160 111 79 22 tfor:TF2829|5end_conserved_hypothetical_protein_3019562..3019792_POSITIVE 309 180 133 229 175 tfor:TF2575|5end_methionine_aminopeptidase_2745126..2745356_POSITIVE 307 159 111 184 135 tfor:TF2318|5end_conserved_hypothetical_protein_2475603..2475833_POSITIVE 305 150 102 66 25 tfor:TF2322|5end_conserved_hypothetical_protein;_possible_lipoprotein_2482743..2482973_POSITIVE 305 140 98 70 24 tfor:TF1758|5end_conserved_hypothetical_protein_1894769..1894999_POSITIVE 304 167 124 176 123 tfor:TF1841|5end_hypothetical_protein_1991936..1992166_POSITIVE 301 160 116 50 2 tfor:TF2631|5end_conserved_hypothetical_protein_2801694..2801924_POSITIVE 301 159 111 153 108 tfor:TF0765|5end_conserved_hypothetical_protein_811302..811532_POSITIVE 300 172 134 186 144 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 295 160 111 99 35 tfor:TF3135|5end_hypothetical_protein_3369821..3370051_POSITIVE 295 172 134 72 30 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 294 165 123 109 49 tfor:TF2187|5end_conserved_hypothetical_protein_2361693..2361923_POSITIVE 293 165 125 93 56 tfor:TF2296|5end_hypothetical_protein_2448892..2449122_POSITIVE 292 149 102 222 158 tfor:TF2906|5end_phosphatidylserine_decarboxylase_3102182..3102412_POSITIVE 292 131 97 207 164 tfor:TF0594|5end_conserved_hypothetical_protein;_possible_PAP2_superfamily_protein_626971..627201_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 312 160 111 79 22 tfor:TF2827|3end_conserved_hypothetical_protein_3019642..3019792_POSITIVE 311 150 107 72 26 tfor:TF2830|3end_ABC_transporter,_ATP-binding_protein_3021419..3021569_POSITIVE 301 159 111 142 97 tfor:TF0764|3end_conserved_hypothetical_protein;_possible_exopolyphosphatase-related_protein_811371..811521_POSITIVE 300 172 134 135 93 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 295 160 111 111 47 tfor:TF3133|3end_conserved_hypothetical_protein_3369913..3370063_POSITIVE 291 146 103 58 12 tfor:TF1757|3end_chromosome_partitioning_protein_1894825..1894975_POSITIVE 289 146 101 98 44 tfor:TF1897|3end_conserved_hypothetical_protein;_possible_aminopeptidase_2052107..2052257_POSITIVE 285 160 112 75 30 tfor:TF0252|3end_hypothetical_protein_283759..283909_POSITIVE 283 167 121 64 22 tfor:TF2339|3end_hypothetical_protein_2505783..2505933_POSITIVE 282 148 110 55 4 tfor:TF0889|3end_conjugative_transposon_protein,_TraO_940078..940228_POSITIVE 280 178 142 120 83 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 280 160 112 56 3 tfor:TF1564|3end_tyrosine-protein_kinase_1672916..1673066_POSITIVE 276 159 111 103 50 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 274 155 111 85 40 tfor:TF2006|3end_uracil_permease_2158642..2158792_POSITIVE 273 147 102 140 95 tfor:TF3124|3end_dTDP-glucose_4,6-dehydratase_3359883..3360033_POSITIVE 271 167 123 75 30 tfor:TF3109|3end_hypothetical_protein_3342803..3342953_POSITIVE 271 162 124 145 121 tfor:TF2703|3end_conserved_hypothetical_protein_2876317..2876467_POSITIVE 271 157 111 145 94 tfor:TF1386|3end_hypothetical_protein_1460488..1460638_POSITIVE 270 160 111 67 8 tfor:TF1930|3end_two-component_response_regulator_2078203..2078353_POSITIVE 269 137 99 47 3 tfor:TF3110|3end_conserved_hypothetical_protein_with_helicase_domain_3346636..3346786_POSITIVE