Origin IGS: gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcgaaattgtttagtgattataatgttctattttgtcgccaaaggtacgatatatttttggcaaagctgctgtctgccctatgcttctttttgcggccgagactacgtgtgaatttagcataaccgtcatgttcctttgattgttatacggttctgtaaaagggcgaacagggcttgttttttcggatggcagataagatcatgattttttttgtatgtttgccattgattcgcattattcgcagaacgagttatacatacaaacggaaacatc Mask Tandem Repeat Region ================================================ gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Find is-nt database================================================ Query_seq: TF1774:TF1775|TF1774:TF1775:arylsulfatase precursor:oxidoreductase, Gfo/Idh/MocA family:->->:1913000..1913274 275 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1774:TF1775|TF1774:TF1775:arylsulfatase precursor:oxidoreductase, Gfo/Idh/MocA family:->->:1913000..1913274 275 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1774:TF1775|TF1774:TF1775:arylsulfatase precursor:oxidoreductase, Gfo/Idh/MocA family:->->:1913000..1913274 275 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 30 End: 128 ............................. M R I M R I N G K H T K K I M I L S A I R K N K P C S P F Y R T V ................................................................................................................................................... Protein_Len: 48 Strand: + Start: 2 End: 145 . M F P F V C I T R S A N N A N Q W Q T Y K K N H D L I C H P K K Q A L F A L L Q N R I T I K G T .................................................................................................................................. Protein_Len: 60 Strand: + Start: 82 End: 261 ................................................................................. M P S E K T S P V R P F T E P Y N N Q R N M T V M L N S H V V S A A K R S I G Q T A A L P K I Y R T F G D K I E H Y N H .............. Protein_Len: 32 Strand: - Start: 166 End: 261 ..................................................................................................................................................................... V Y D R G C F S A Y P L C C S Q W F Y I T G K A V F Y F M I I M .............. Protein_Len: 38 Strand: - Start: 155 End: 268 .......................................................................................................................................................... A L N V R L R P R L F F C L A S L L K A L F I D Y R Q R C F L V N Y D S F M ....... Protein_Len: 36 Strand: - Start: 138 End: 245 ......................................................................................................................................... L F M V T I S F E C T T E A A F L L M P C V A A K G F I Y R V K P S L M .............................. Protein_Len: 33 Strand: - Start: 2 End: 100 . H K R K Y T Y S T R R I I R I L P L C V F F I M I K D A M R F F M ............................................................................................................................................................................... gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1774:TF1775|TF1774:TF1775:arylsulfatase precursor:oxidoreductase, Gfo/Idh/MocA family:->->:1913000..1913274 275 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Intra-Species Hit: Count: 1 Min: 1 Max: 275 Len: 275 Subject: tfor_TF1774_TF1775|arylsulfatase precursor:oxidoreductase, Gfo/Idh/MocA family|POSITIVE:POSITIVE|[1913000,1913274]|275 HSP 1 e-value: 1.0E-140 bit: 498.0 Len: 275 Query Start:1 Query End:275 Subject Strand: POSITIVE Subject Start: 1 Subject End: 275 gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacnnnnnnnntcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat gatgtttccgtttgtatgtataactcgttctgcgaataatgcgaatcaatggcaaacatacaaaaaaaatcatgatcttatctgccatccgaaaaaacaagccctgttcgcccttttacagaaccgtataacaatcaaaggaacatgacggttatgctaaattcacacgtagtctcggccgcaaaaagaagcatagggcagacagcagctttgccaaaaatatatcgtacctttggcgacaaaatagaacattataatcactaaacaatttcgat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGUUUCCGUUUGUAUGUAUAACUCGUUCUGCGAAUAAUGCGAAUCAAUGGCAAACAUACAAAAAAAAUCAUGAUCUUAUCUGCCAUCCGAAAAAACAAGCCCUGUUCGCCCUUUUACAGAACCGUAUAACAAUCAAAGGAACAUGACGGUUAUGCUAAAUUCACACGUAGUCUCGGCCGCAAAAAGAAGCAUAGGGCAGACAGCAGCUUUGCCAAAAAUAUAUCGUACCUUUGGCGACAAAAUAGAACAUUAUAAUCACUAAACAAUUUCGAU .(((((((((((.((.(((((.(.(((.....))).).))))).)).))))).))))))..............................(((((.....(((.((((..(((((...........(((((((..(((........)))..)))))))...........(..((....))..)............)))))..))))..)))((((((((..(((...)))..)))))))).............................))))).. (-55.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCGAAAUUGUUUAGUGAUUAUAAUGUUCUAUUUUGUCGCCAAAGGUACGAUAUAUUUUUGGCAAAGCUGCUGUCUGCCCUAUGCUUCUUUUUGCGGCCGAGACUACGUGUGAAUUUAGCAUAACCGUCAUGUUCCUUUGAUUGUUAUACGGUUCUGUAAAAGGGCGAACAGGGCUUGUUUUUUCGGAUGGCAGAUAAGAUCAUGAUUUUUUUUGUAUGUUUGCCAUUGAUUCGCAUUAUUCGCAGAACGAGUUAUACAUACAAACGGAAACAUC .......((((((.((((..(((((((...........((((((((((.....)))))))))).(((((.((((.((((((.........((((((((((...(((.((....)).))).(((((.((((........)))).))))).)))..))))))))))))).))))))))).........(((((((((((....((.((....)).))..))))))))))).....))))))))))).))))))...............(....)... (-70.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 209 161 63 3 tfor:TF2637|5end_conserved_hypothetical_protein_2807144..2807374_POSITIVE 362 216 172 121 62 tfor:TF2079|5end_permease_2245585..2245815_POSITIVE 353 216 171 162 108 tfor:TF0023|5end_possible_response_element_in_two_component_regulation_19446..19676_POSITIVE 343 208 161 141 82 tfor:TF2279|5end_hypothetical_protein_2438895..2439125_POSITIVE 341 207 166 78 35 tfor:TF0248|5end_hypothetical_protein_278249..278479_POSITIVE 334 204 168 191 154 tfor:TF1586|5end_hypothetical_protein_1690971..1691201_POSITIVE 331 213 176 44 1 tfor:TF2704|5end_ribonuclease_HII_2876242..2876472_POSITIVE 331 209 168 200 154 tfor:TF2187|5end_conserved_hypothetical_protein_2361693..2361923_POSITIVE 330 209 163 65 13 tfor:TF2780|5end_hypothetical_protein_2966060..2966290_POSITIVE 330 240 191 155 88 tfor:TF1722|5end_conserved_hypothetical_protein_1846010..1846240_POSITIVE 320 126 81 65 25 tfor:TF3143|5end_conserved_hypothetical_protein;_possible_membrane_protein_3377465..3377695_POSITIVE 320 215 171 126 94 tfor:TF0992|5end_hypothetical_protein_1048251..1048481_POSITIVE 319 216 176 85 45 tfor:TF2750|5end_L-serine_dehydratase_2934432..2934662_POSITIVE 317 208 167 64 20 tfor:TF2021|5end_hypothetical_protein_2177702..2177932_POSITIVE 316 218 172 126 57 tfor:TF1624|5end_carboxyl-terminal_protease_1737252..1737482_POSITIVE 316 238 191 77 31 tfor:TF1170|5end_conserved_hypothetical_protein_1251862..1252092_POSITIVE 316 238 191 65 19 tfor:TF1171|5end_ABC_transporter,_ATP-binding_protein_1251850..1252080_POSITIVE 316 208 163 43 7 tfor:TF0826|5end_primosomal_protein_N'_883751..883981_POSITIVE 314 128 82 47 6 tfor:TF2165|5end_hypothetical_protein_2341654..2341884_POSITIVE 311 238 192 167 118 tfor:TF2775|5end_conserved_hypothetical_protein;_possible_mucoidy_inhibitor-related_protein_2959668..2959898_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 220 172 82 39 tfor:TF2613|3end_conserved_hypothetical_protein_2789798..2789948_POSITIVE 341 207 166 97 54 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 332 203 162 64 21 tfor:TF1382|3end_hypothetical_protein_1457777..1457927_POSITIVE 332 203 162 60 17 tfor:TF1383|3end_hypothetical_protein_1457773..1457923_POSITIVE 330 240 191 87 20 tfor:TF1721|3end_2,3-bisphosphoglycerate-independent_phosphoglycerate_mutase_1846022..1846172_POSITIVE 320 215 171 129 97 tfor:TF0991|3end_probable_NAD(FAD)-dependent_dehydrogenase_1048334..1048484_POSITIVE 319 216 176 91 51 tfor:TF2749|3end_possible_hemolysin_2934518..2934668_POSITIVE 316 218 172 126 57 tfor:TF1623|3end_5-formyltetrahydrofolate_cyclo-ligase_1737332..1737482_POSITIVE 316 208 163 62 26 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 311 238 192 138 89 tfor:TF2774|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_2959719..2959869_POSITIVE 310 210 169 148 106 tfor:TF2003|3end_dihydrofolate_reductase_2156427..2156577_POSITIVE 308 209 161 149 97 tfor:TF2278|3end_conserved_hypothetical_protein_2438990..2439140_POSITIVE 304 216 171 151 100 tfor:TF0596|3end_conserved_hypothetical_protein_628652..628802_POSITIVE 303 185 146 64 20 tfor:TF0073|3end_possible_dTDP-glucose_4,6-dehydratase,_NAD-dependent_epimerase/dehydratase_family_82051..82201_POSITIVE 299 199 151 84 28 tfor:TF2696|3end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_2871611..2871761_POSITIVE 299 210 167 142 101 tfor:TF0777|3end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_827053..827203_POSITIVE 298 215 172 92 37 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 298 203 167 64 25 tfor:TF1197|3end_transcriptional_regulator_1281414..1281564_POSITIVE 296 208 167 61 5 tfor:TF2379|3end_phosphoribosylformylglycinamidine_cyclo-ligase_(AIR_synthase)_2545092..2545242_POSITIVE 295 217 171 67 9 tfor:TF1817|3end_hypothetical_protein_1957991..1958141_POSITIVE