Origin IGS: tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgtatctttaaaaatactttagtatatagcagaaagagactaaagaatactgtctataagattgattttgctctatctttgcaacaaagttctatgtcattgtggttatctcattgatgttgcgaattgaatgtttagctaaattattttaagctatgttacaattagatcaaaatctaagttttgttagggtttctcatgaagatgctgttagtgttgcagacttgaatgtaactgcagatgatgttggctcagaagtcgctcctggttgctgtggaacctttgggacctttggaacagcgggagggtgttgcggaactttcggcacatatggatgtgcgggaggagctgagataacacaactgtgaaccaggtcttaacgtctgtaccga Mask Tandem Repeat Region ================================================ tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Find is-nt database================================================ Query_seq: TF1750:TF1751|TF1750:TF1751:hypothetical protein:two-component system response regulator:->->:1886627..1887021 395 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1750:TF1751|TF1750:TF1751:hypothetical protein:two-component system response regulator:->->:1886627..1887021 395 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1750:TF1751|TF1750:TF1751:hypothetical protein:two-component system response regulator:->->:1886627..1887021 395 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 31 End: 138 .............................. M C Y L S S S R T S I C A E S S A T P S R C S K G P K G S T A T R S D F ................................................................................................................................................................................................................................................................. Protein_Len: 49 Strand: + Start: 33 End: 179 ................................ M L S Q L L P H I H M C R K F R N T L P L F Q R S Q R F H S N Q E R L L S Q H H L Q L H S S L Q H ........................................................................................................................................................................................................................ Protein_Len: 58 Strand: + Start: 20 End: 193 ................... M V H S C V I S A P P A H P Y V P K V P Q H P P A V P K V P K V P Q Q P G A T S E P T S S A V T F K S A T L T A S S .......................................................................................................................................................................................................... Protein_Len: 37 Strand: - Start: 279 End: 389 ...................................................................................................................................................................................................................................................................................... H S L W L S M S S Q Q L S L A F D I K Y V T N K L R K R S Y V L T N K F M ...... Protein_Len: 40 Strand: - Start: 39 End: 158 ...................................... R L E E R V D M H A S L E A V G E R Q E L P G L P E V A V L L S K Q A L M M Q M ............................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 28 End: 237 ........................... G Y T G F T G C C G G A T G F T G F T G C C G P A V E S G V D D A T V N L D A V S V A D E H S V R V F S L N Q D L Q L M .............................................................................................................................................................. tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 44 HP_score: -2.6 Tail_Score: -3.09968 Start: 237 End: 257 Full_Region: tgcgaattgaatgtt tagctaaa ttatt ttaagcta tgttacaattagatc ............................................................................................................................................................................................................................................tagctaaattattttaagcta.......................................................................................................................................... Find igs database================================================ Query_seq: TF1750:TF1751|TF1750:TF1751:hypothetical protein:two-component system response regulator:->->:1886627..1887021 395 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Intra-Species Hit: Count: 1 Min: 1 Max: 395 Len: 395 Subject: tfor_TF1750_TF1751|hypothetical protein:two-component system response regulator|POSITIVE:POSITIVE|[1886627,1887021]|395 HSP 1 e-value: 0.0 bit: 783.0 Len: 395 Query Start:1 Query End:395 Subject Strand: POSITIVE Subject Start: 1 Subject End: 395 tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta tcggtacagacgttaagacctggttcacagttgtgttatctcagctcctcccgcacatccatatgtgccgaaagttccgcaacaccctcccgctgttccaaaggtcccaaaggttccacagcaaccaggagcgacttctgagccaacatcatctgcagttacattcaagtctgcaacactaacagcatcttcatgagaaaccctaacaaaacttagattttgatctaattgtaacatagcttaaaataatttagctaaacattcaattcgcaacatcaatgagataaccacaatgacatagaactttgttgcaaagatagagcaaaatcaatcttatagacagtattctttagtctctttctgctatatactaaagtatttttaaagatacgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCGGUACAGACGUUAAGACCUGGUUCACAGUUGUGUUAUCUCAGCUCCUCCCGCACAUCCAUAUGUGCCGAAAGUUCCGCAACACCCUCCCGCUGUUCCAAAGGUCCCAAAGGUUCCACAGCAACCAGGAGCGACUUCUGAGCCAACAUCAUCUGCAGUUACAUUCAAGUCUGCAACACUAACAGCAUCUUCAUGAGAAACCCUAACAAAACUUAGAUUUUGAUCUAAUUGUAACAUAGCUUAAAAUAAUUUAGCUAAACAUUCAAUUCGCAACAUCAAUGAGAUAACCACAAUGACAUAGAACUUUGUUGCAAAGAUAGAGCAAAAUCAAUCUUAUAGACAGUAUUCUUUAGUCUCUUUCUGCUAUAUACUAAAGUAUUUUUAAAGAUACGUUA ..(((.((((.(((....(((((((....((((((..((((((((.......((((((....)))))).....(((....)))........)))).......(....)..))))..)))))))))))))...))).)))).))).........(((((..((......)))))))........((((..(((((((.........((((...((((((....))))))))))....(((((...........)))))..................)).)))))...........(((..((((..(((((...(((((.((......)).)))))...)))))..))))...)))......))))..........((((((....)))))).... (-80.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGUAUCUUUAAAAAUACUUUAGUAUAUAGCAGAAAGAGACUAAAGAAUACUGUCUAUAAGAUUGAUUUUGCUCUAUCUUUGCAACAAAGUUCUAUGUCAUUGUGGUUAUCUCAUUGAUGUUGCGAAUUGAAUGUUUAGCUAAAUUAUUUUAAGCUAUGUUACAAUUAGAUCAAAAUCUAAGUUUUGUUAGGGUUUCUCAUGAAGAUGCUGUUAGUGUUGCAGACUUGAAUGUAACUGCAGAUGAUGUUGGCUCAGAAGUCGCUCCUGGUUGCUGUGGAACCUUUGGGACCUUUGGAACAGCGGGAGGGUGUUGCGGAACUUUCGGCACAUAUGGAUGUGCGGGAGGAGCUGAGAUAACACAACUGUGAACCAGGUCUUAACGUCUGUACCGA (((((((((((((...((((....)))).((((((((..((((...........))))........(((((((.((((..(((((((((..........(((.((.(.....).)).)))))))))))).....(((.((((((((....))).)))))....)))..)))).)))))))....))))))))..........))))))))).))))..((((((........)))))).(((((((.(((((.(((((...((((.(((..(((((((....((...(....)...)).)))))))..)))....))))..(((((.((((((....)))))).)))))..))))).)))))...(((.....))).......)))))))..... (-106.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 410 90 43 213 157 tfor:TF1322|5end_conserved_hypothetical_protein;_possible_surface_protein_1401864..1402094_POSITIVE 397 98 51 185 144 tfor:TF2661|5end_hypothetical_protein_2833509..2833739_POSITIVE 389 100 51 148 85 tfor:TF2295|5end_hypothetical_protein_2448164..2448394_POSITIVE 374 158 111 125 80 tfor:TF2660|5end_hypothetical_protein_2833293..2833523_POSITIVE 360 129 81 200 147 tfor:TF2789|5end_enoyl-[acyl-carrier-protein]_reductase_2972431..2972661_POSITIVE 357 110 61 212 155 tfor:TF1979|5end_conserved_hypothetical_protein_2127957..2128187_POSITIVE 354 90 41 59 6 tfor:TF1548|5end_thiophene_and_furan_oxidation_protein_1652501..1652731_POSITIVE 351 89 42 78 24 tfor:TF2336|5end_conserved_hypothetical_protein_2496169..2496399_POSITIVE 347 80 31 194 146 tfor:TF2781|5end_hypothetical_protein_2965822..2966052_POSITIVE 347 129 83 125 89 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 344 90 44 211 150 tfor:TF1763|5end_K+-dependent_Na+/Ca+_exchanger_related-protein_1900406..1900636_POSITIVE 342 100 51 206 162 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 341 81 41 85 40 tfor:TF2159|5end_conserved_hypothetical_protein_2334446..2334676_POSITIVE 340 89 41 220 168 tfor:TF2772|5end_tRNA_pseudouridine_synthase_B_2957383..2957613_POSITIVE 339 90 43 62 15 tfor:TF2759|5end_xylose_repressor,_ROK_family_2943568..2943798_POSITIVE 339 138 95 217 159 tfor:TF2183|5end_hypothetical_protein_2355362..2355592_POSITIVE 339 135 91 125 71 tfor:TF1312|5end_hypothetical_protein_1388465..1388695_POSITIVE 339 135 91 125 71 tfor:TF1311|5end_hypothetical_protein_1388242..1388472_POSITIVE 338 90 44 200 145 tfor:TF3096|5end_conserved_hypothetical_protein_3327963..3328193_POSITIVE 338 83 43 214 176 tfor:TF2044|5end_excinuclease_ABC,_subunit_A_2203228..2203458_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 397 98 51 136 95 tfor:TF2660|3end_hypothetical_protein_2833540..2833690_POSITIVE 362 159 114 150 106 tfor:TF0128|3end_hypothetical_protein_140556..140706_POSITIVE 352 105 68 105 52 tfor:TF2366|3end_conserved_hypothetical_protein_2531892..2532042_POSITIVE 347 129 83 125 89 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 341 81 41 143 98 tfor:TF2158|3end_conserved_hypothetical_protein_2334584..2334734_POSITIVE 339 135 91 138 84 tfor:TF1311|3end_hypothetical_protein_1388558..1388708_POSITIVE 339 135 91 138 84 tfor:TF1310|3end_hypothetical_protein_1388335..1388485_POSITIVE 338 81 42 109 65 tfor:TF0242|3end_conserved_hypothetical_protein_274069..274219_POSITIVE 330 70 21 121 76 tfor:TF3124|3end_dTDP-glucose_4,6-dehydratase_3359883..3360033_POSITIVE 327 118 75 48 1 tfor:TF1109|3end_lipoprotein_1179997..1180147_POSITIVE 326 109 64 46 4 tfor:TF0037|3end_sialic_acid-specific_9-O-acetylesterase_39991..40141_POSITIVE 325 135 91 138 87 tfor:TF1312|3end_hypothetical_protein_1388781..1388931_POSITIVE 321 99 51 128 85 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 318 88 41 136 96 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 311 90 41 150 93 tfor:TF2257|3end_transfer_region-related_protein,_TraJ_2429622..2429772_POSITIVE 311 94 51 64 12 tfor:TF1930|3end_two-component_response_regulator_2078203..2078353_POSITIVE 309 70 29 124 79 tfor:TF1622|3end_conserved_hypothetical_protein_1736763..1736913_POSITIVE 307 96 51 150 91 tfor:TF2294|3end_hypothetical_protein_2448250..2448400_POSITIVE 307 42 2 45 1 tfor:TF0932|3end_hypothitical_protein_972477..972627_POSITIVE 306 74 31 49 10 tfor:TF1438|3end_hypothetical_protein_1522524..1522674_POSITIVE