Origin IGS: agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagtgattcaagatatcttggaacgatatcgctcagcctttcatcccccaaaattatgaatgcgttaaagatggttatgctctccactcatcagagaaaaatagcttgaaatcatttccgtttttgattggaggaaaaagatacaccacgaattttcagaagcatagatcatgataaattaatgttttctcccggttatcctgtaatgaaacgtttttcaaacatacacaccacattttttttgtagatttgtcagccgaaaaaataaatgaactgaactttctaatcatacaatttatct Mask Tandem Repeat Region ================================================ agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Find is-nt database================================================ Query_seq: TF1735:TF1736|TF1735:TF1736:dehydrogenase, possible NADH-dependent dehydrogenase:hypothetical protein:->->:1861521..1861822 302 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1735:TF1736|TF1735:TF1736:dehydrogenase, possible NADH-dependent dehydrogenase:hypothetical protein:->->:1861521..1861822 302 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1735:TF1736|TF1735:TF1736:dehydrogenase, possible NADH-dependent dehydrogenase:hypothetical protein:->->:1861521..1861822 302 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 3 End: 143 .. M N C M I R K F S S F I F S A D K S T K K M W C V C L K N V S L Q D N R E K T L I Y H D L C F ............................................................................................................................................................... Protein_Len: 36 Strand: + Start: 127 End: 234 .............................................................................................................................. M M I Y A S E N S W C I F F L Q S K T E M I S S Y F S L M S G E H N H L .................................................................... Protein_Len: 53 Strand: - Start: 131 End: 289 .................................................................................................................................. S R H K Q F N T T Y R K G G I L F P F S K L S N K E S S H L A Y G D K V C E Y N Q P I F P Q A I D N W S M ............. Protein_Len: 39 Strand: - Start: 58 End: 174 ......................................................... L F F T T H I N S F R K M V P Y G P S F M L K D H D I S R F I R P T D K E E M ................................................................................................................................ Protein_Len: 41 Strand: - Start: 2 End: 124 . I F Q I I L F N L E N I K E A S L D V F F I H H T H K F F T E N C S L R S F V N M .................................................................................................................................................................................. agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1735:TF1736|TF1735:TF1736:dehydrogenase, possible NADH-dependent dehydrogenase:hypothetical protein:->->:1861521..1861822 302 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Intra-Species Hit: Count: 1 Min: 1 Max: 302 Len: 302 Subject: tfor_TF1735_TF1736|dehydrogenase, possible NADH-dependent dehydrogenase:hypothetical protein|POSITIVE:POSITIVE|[1861521,1861822]|302 HSP 1 e-value: 1.0E-156 bit: 551.0 Len: 302 Query Start:1 Query End:302 Subject Strand: POSITIVE Subject Start: 1 Subject End: 302 agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacnnnnnnnntgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta agataaattgtatgattagaaagttcagttcatttattttttcggctgacaaatctacaaaaaaaatgtggtgtgtatgtttgaaaaacgtttcattacaggataaccgggagaaaacattaatttatcatgatctatgcttctgaaaattcgtggtgtatctttttcctccaatcaaaaacggaaatgatttcaagctatttttctctgatgagtggagagcataaccatctttaacgcattcataattttgggggatgaaaggctgagcgatatcgttccaagatatcttgaatcactta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUAAAUUGUAUGAUUAGAAAGUUCAGUUCAUUUAUUUUUUCGGCUGACAAAUCUACAAAAAAAAUGUGGUGUGUAUGUUUGAAAAACGUUUCAUUACAGGAUAACCGGGAGAAAACAUUAAUUUAUCAUGAUCUAUGCUUCUGAAAAUUCGUGGUGUAUCUUUUUCCUCCAAUCAAAAACGGAAAUGAUUUCAAGCUAUUUUUCUCUGAUGAGUGGAGAGCAUAACCAUCUUUAACGCAUUCAUAAUUUUGGGGGAUGAAAGGCUGAGCGAUAUCGUUCCAAGAUAUCUUGAAUCACUUA ....................((((...(((((.........((((((.....(((..(...((((.((((((((((..((((((((..((((((.......(((....((((((((((((((..((.(((.((........))))).))....))))))...))))))))...))).......)))))).)))))))).....((((((.......))))))..............))))).))))).)))).)..)))....))))))..((((((.......)))))).))))).)))). (-62.84) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUGAUUCAAGAUAUCUUGGAACGAUAUCGCUCAGCCUUUCAUCCCCCAAAAUUAUGAAUGCGUUAAAGAUGGUUAUGCUCUCCACUCAUCAGAGAAAAAUAGCUUGAAAUCAUUUCCGUUUUUGAUUGGAGGAAAAAGAUACACCACGAAUUUUCAGAAGCAUAGAUCAUGAUAAAUUAAUGUUUUCUCCCGGUUAUCCUGUAAUGAAACGUUUUUCAAACAUACACACCACAUUUUUUUUGUAGAUUUGUCAGCCGAAAAAAUAAAUGAACUGAACUUUCUAAUCAUACAAUUUAUCU ...((((((...((((((.......)))))).......((((..(((.(((..((((.....(((..((.(((((((((..((((.........))))...))))))....))).))..)))...))))))).))).)))).........(((..(((((...(((.....(((.......((((((((.(..(((.....)))..).)))))))).......))).........((((((((.((.((....)).)).))))))))..)))..)))))..))).))))))........... (-48.14) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 337 170 128 150 113 tfor:TF0309|5end_conserved_hypothetical_protein_334174..334404_POSITIVE 304 282 251 211 180 tfor:TF2306|5end_hypothetical_protein_2466674..2466904_POSITIVE 301 270 222 55 14 tfor:TF1286|5end_hypothetical_protein_1369199..1369429_POSITIVE 294 258 211 58 24 tfor:TF0801|5end_transport_permease_858586..858816_POSITIVE 292 297 251 158 113 tfor:TF1898|5end_RNA_polymerase_ECF-type_sigma_factor_2052115..2052345_POSITIVE 287 290 242 56 1 tfor:TF1857|5end_conserved_hypothetical_protein_2014366..2014596_POSITIVE 286 144 106 102 69 tfor:TF1730|5end_diaminopimelate_decarboxylase_1854670..1854900_POSITIVE 286 144 106 51 18 tfor:TF1732|5end_conserved_hypothetical_protein_1854619..1854849_POSITIVE 284 298 252 55 12 tfor:TF2040|5end_transcription_elongation_factor_2199733..2199963_POSITIVE 284 199 161 181 138 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 283 219 182 153 123 tfor:TF2087|5end_conserved_hypothetical_protein_2256856..2257086_POSITIVE 283 290 244 137 89 tfor:TF0381|5end_cell_cycle_protein_401632..401862_POSITIVE 280 295 252 79 41 tfor:TF2821|5end_conserved_hypothetical_protein_3011146..3011376_POSITIVE 280 280 238 126 74 tfor:TF0463|5end_precorrin-6Y_C5,15-methyltransferase_479015..479245_POSITIVE 278 280 232 52 7 tfor:TF1609|5end_hypothetical_protein_1728340..1728570_POSITIVE 277 240 197 66 21 tfor:TF2211|5end_conserved_hypothetical_protein_2384392..2384622_POSITIVE 277 270 221 228 191 tfor:TF1395|5end_signal_peptidase_I_1468266..1468496_POSITIVE 277 290 251 217 166 tfor:TF1304|5end_conserved_hypothetical_protein_1385008..1385238_POSITIVE 275 283 251 54 23 tfor:TF1112|5end_possible_response_regulator,_LytR/AlgR_family_1182009..1182239_POSITIVE 273 281 250 64 31 tfor:TF1935|5end_ATP-dependent_RNA_helicase,_DEAD/DEAH-related_helicase_2082002..2082232_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 297 251 70 25 tfor:TF1897|3end_conserved_hypothetical_protein;_possible_aminopeptidase_2052107..2052257_POSITIVE 286 144 106 70 37 tfor:TF1729|3end_aspartokinase_1854718..1854868_POSITIVE 284 298 252 77 34 tfor:TF2039|3end_possible_cell-cycle_regulation_histidine_triad_(HIT)_protein_2199835..2199985_POSITIVE 284 199 161 104 61 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 283 300 252 56 5 tfor:TF1078|3end_possible_molybdenum_cofactor_biosynthesis_enzyme_1148473..1148623_POSITIVE 283 290 244 58 10 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE 280 295 252 53 15 tfor:TF2820|3end_argininosuccinate_synthetase_3011200..3011350_POSITIVE 280 280 238 126 74 tfor:TF0462|3end_possible_cobalamin_biosynthesis_precorrin-3_methylase_479095..479245_POSITIVE 278 280 232 95 50 tfor:TF1608|3end_sulfate_transporter_1728463..1728613_POSITIVE 275 292 252 128 82 tfor:TF2918|3end_hypothetical_protein_3114382..3114532_POSITIVE 272 109 66 76 28 tfor:TF2673|3end_conserved_hypothetical_protein_2848272..2848422_POSITIVE 270 110 76 39 1 tfor:TF0721|3end_aspartate_transaminase_772665..772815_POSITIVE 269 281 252 125 98 tfor:TF1351|3end_conserved_hypothetical_protein_1432050..1432200_POSITIVE 267 226 189 52 4 tfor:TF2938|3end_conserved_hypothetical_protein;_possible_translocase_secE_subunit_3140828..3140978_POSITIVE 266 224 181 67 11 tfor:TF3026|3end_conserved_hypothetical_protein_3250254..3250404_POSITIVE 265 110 66 103 54 tfor:TF2342|3end_glutathione_peroxidase_2506639..2506789_POSITIVE 262 284 249 115 75 tfor:TF1075|3end_conserved_hypothetical_protein;_possible_lantibiotic_biosynthesis_protein_1145401..1145551_POSITIVE 261 235 206 72 42 tfor:TF2457|3end_ABC_transporter,_permease_component,_zinc-binding_protein_2635302..2635452_POSITIVE 258 223 183 63 24 tfor:TF2894|3end_conserved_hypothetical_protein;_possible_Fic_family_protein_3083587..3083737_POSITIVE 258 284 248 132 90 tfor:TF1814|3end_conserved_hypothetical_protein;_possible_biotin_synthase_related_domain_containing_protein_1956670..1956820_POSITIVE